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Residual matrix from different separation techniques impacts exosome biological activity
Scientific Reports, ISSN 2045-2322, 03/2016, Volume 6, Issue 1, p. 23550
Exosomes are gaining a prominent role in research due to their intriguing biology and several therapeutic opportunities. However, their accurate purification...
MICROVESICLES | PLASMA | EXTRACELLULAR VESICLES | HUMAN-SALIVA | CANCER | MULTIDISCIPLINARY SCIENCES | Exosomes - metabolism | Cell Nucleus - metabolism | Triiodobenzoic Acids - pharmacology | Humans | Cell Fractionation - methods | Sucrose - pharmacology | NF-kappa B - metabolism | Centrifugation, Density Gradient - methods | Microscopy, Atomic Force - methods | Multiple Myeloma - metabolism | Multiple Myeloma - blood | Protein Transport | Atomic force microscopy | NF-κB protein | Scanning | Purification | Sucrose | Centrifugation | Multiple myeloma | Embedding | Helium | Exosomes | Biological activity | Nuclear transport | Endothelial cells | Body fluids | Microscopy | Contaminants | Information processing | Separation techniques
MICROVESICLES | PLASMA | EXTRACELLULAR VESICLES | HUMAN-SALIVA | CANCER | MULTIDISCIPLINARY SCIENCES | Exosomes - metabolism | Cell Nucleus - metabolism | Triiodobenzoic Acids - pharmacology | Humans | Cell Fractionation - methods | Sucrose - pharmacology | NF-kappa B - metabolism | Centrifugation, Density Gradient - methods | Microscopy, Atomic Force - methods | Multiple Myeloma - metabolism | Multiple Myeloma - blood | Protein Transport | Atomic force microscopy | NF-κB protein | Scanning | Purification | Sucrose | Centrifugation | Multiple myeloma | Embedding | Helium | Exosomes | Biological activity | Nuclear transport | Endothelial cells | Body fluids | Microscopy | Contaminants | Information processing | Separation techniques
Journal Article
Analytical Chemistry, ISSN 0003-2700, 07/2018, Volume 90, Issue 13, p. 7855
Understanding extracellular vesicle (EV) internalization mechanisms and pathways in cells is of capital importance for both EV basic biology and clinical...
Cellular biology | Diagnostics | Diagnostic software | Purity | Diagnostic systems | Biology | Colorimetry | Tumors | Cancer
Cellular biology | Diagnostics | Diagnostic software | Purity | Diagnostic systems | Biology | Colorimetry | Tumors | Cancer
Journal Article
Journal of Extracellular Vesicles, ISSN 2001-3078, 12/2018, Volume 7, Issue 1, pp. 1535750 - 43
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular...
extracellular vesicles | rigor | exosomes | reproducibility | guidelines | standardization | microparticles | microvesicles | minimal information requirements | ectosomes | HUMAN URINARY EXOSOMES | FETAL BOVINE | PREANALYTICAL PARAMETERS | CELL-DERIVED MICROPARTICLES | CELL BIOLOGY | FIELD-FLOW FRACTIONATION | MEMBRANE-VESICLES | PROSTATE-CANCER | SURFACE-PLASMON RESONANCE | SIZE-EXCLUSION CHROMATOGRAPHY | CIRCULATING MICROPARTICLES | Vesicles | Physiology | Biosynthesis | Scientists | Metastasis | Exosomes | Microparticles | Polls & surveys | Cancer | Apoptosis | Membranes cel·lulars | Cell interaction | Cell membranes | Interacció cel·lular | Cellbiologi | Cell Biology
extracellular vesicles | rigor | exosomes | reproducibility | guidelines | standardization | microparticles | microvesicles | minimal information requirements | ectosomes | HUMAN URINARY EXOSOMES | FETAL BOVINE | PREANALYTICAL PARAMETERS | CELL-DERIVED MICROPARTICLES | CELL BIOLOGY | FIELD-FLOW FRACTIONATION | MEMBRANE-VESICLES | PROSTATE-CANCER | SURFACE-PLASMON RESONANCE | SIZE-EXCLUSION CHROMATOGRAPHY | CIRCULATING MICROPARTICLES | Vesicles | Physiology | Biosynthesis | Scientists | Metastasis | Exosomes | Microparticles | Polls & surveys | Cancer | Apoptosis | Membranes cel·lulars | Cell interaction | Cell membranes | Interacció cel·lular | Cellbiologi | Cell Biology
Journal Article
Angewandte Chemie International Edition, ISSN 1433-7851, 08/2014, Volume 53, Issue 35, pp. 9183 - 9188
The direct, clean, and unbiased transduction of molecular recognition into a readable and reproducible response is the biggest challenge associated to the use...
illicit drugs | molecular recognition | cavitands | cantilevers | designer drugs | HAIR | NANOMECHANICS | SENSORS | CHEMISTRY, MULTIDISCIPLINARY | THERMODYNAMICS | 7-AMINOFLUNITRAZEPAM | SURFACE | FLUNITRAZEPAM | Substance Abuse Detection - methods | Designer Drugs - chemistry | Methamphetamine - analysis | Designer Drugs - analysis | Water - chemistry | Molecular Structure | Methamphetamine - chemistry | Silicon - chemistry | Illegal drugs | Silicon | Investigations | Drugs | Receptors | Methamphetamine | Nanostructure | Illicit | Devices | Recognition
illicit drugs | molecular recognition | cavitands | cantilevers | designer drugs | HAIR | NANOMECHANICS | SENSORS | CHEMISTRY, MULTIDISCIPLINARY | THERMODYNAMICS | 7-AMINOFLUNITRAZEPAM | SURFACE | FLUNITRAZEPAM | Substance Abuse Detection - methods | Designer Drugs - chemistry | Methamphetamine - analysis | Designer Drugs - analysis | Water - chemistry | Molecular Structure | Methamphetamine - chemistry | Silicon - chemistry | Illegal drugs | Silicon | Investigations | Drugs | Receptors | Methamphetamine | Nanostructure | Illicit | Devices | Recognition
Journal Article
Acta Materialia, ISSN 1359-6454, 04/2006, Volume 54, Issue 7, pp. 1843 - 1849
A microwave (MW) field can induce in a dielectric material an oscillatory polarization. By this mechanism part of the energy carried by the waves is converted...
Thermodynamics | Microwaves | Dielectrics | dielectrics | microwaves | CAPACITY | DEPENDENT SPECIFIC-HEAT | MYTHS AUTOPSY | ELECTRIC-FIELD | MATERIALS SCIENCE, MULTIDISCIPLINARY | METALLURGY & METALLURGICAL ENGINEERING | thermodynamics | GLASS-TRANSITION | SPECTROSCOPY | MODULATED TEMPERATURE DSC | CHEMISTRY | DIFFUSION | LIQUIDS | Heating, ventilation, and air conditioning industry | Microwave devices
Thermodynamics | Microwaves | Dielectrics | dielectrics | microwaves | CAPACITY | DEPENDENT SPECIFIC-HEAT | MYTHS AUTOPSY | ELECTRIC-FIELD | MATERIALS SCIENCE, MULTIDISCIPLINARY | METALLURGY & METALLURGICAL ENGINEERING | thermodynamics | GLASS-TRANSITION | SPECTROSCOPY | MODULATED TEMPERATURE DSC | CHEMISTRY | DIFFUSION | LIQUIDS | Heating, ventilation, and air conditioning industry | Microwave devices
Journal Article
Chemical Communications, ISSN 1359-7345, 2018, Volume 54, Issue 52, pp. 7219 - 7222
The recognition of the biological, diagnostic andmedical importance of exosomes has given rise to an urgent need for efficient labelling of these extracellular...
EXTRACELLULAR VESICLES | CHEMISTRY, MULTIDISCIPLINARY | Flow cytometry | Labelling | Diagnostic systems | Fluorescent indicators
EXTRACELLULAR VESICLES | CHEMISTRY, MULTIDISCIPLINARY | Flow cytometry | Labelling | Diagnostic systems | Fluorescent indicators
Journal Article
Analytical Chemistry, ISSN 0003-2700, 07/2018, Volume 90, Issue 13, pp. 7855 - 7861
Understanding extracellular vesicle (EV) internalization mechanisms and pathways in cells is of capital importance for both EV basic biology and clinical...
NANOPARTICLES | CHEMISTRY, ANALYTICAL | EXTRACELLULAR VESICLES | STABILITY | MEMBRANES | REGION | LIPIDS | Exosomes - metabolism | Flow Cytometry - methods | Animals | Biological Transport | Nanotechnology - methods | Colloids | Humans | Cell Line, Tumor | Mice
NANOPARTICLES | CHEMISTRY, ANALYTICAL | EXTRACELLULAR VESICLES | STABILITY | MEMBRANES | REGION | LIPIDS | Exosomes - metabolism | Flow Cytometry - methods | Animals | Biological Transport | Nanotechnology - methods | Colloids | Humans | Cell Line, Tumor | Mice
Journal Article
08/2013, Methods in Molecular Biology Ser., ISBN 9781627034616, Volume 1025
Annotation The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications....
Technology & Engineering | Biotechnology | Science | Nanotechnology
Technology & Engineering | Biotechnology | Science | Nanotechnology
Web Resource
Nanoscale, ISSN 2040-3364, 05/2014, Volume 6, Issue 12, pp. 6452 - 6457
Freestanding lipid bilayers were challenged with 15 nm Au nanospheres either coated by a citrate layer or passivated by a protein corona. The effect of Au...
Nanoparticles | Gold | Membranes | Analogies | Nanospheres | Citrates | Lipids | Nanostructure
Nanoparticles | Gold | Membranes | Analogies | Nanospheres | Citrates | Lipids | Nanostructure
Journal Article
Nanoscale, ISSN 2040-3364, 06/2014, Volume 6, Issue 12, pp. 6452 - 6457
Freestanding lipid bilayers were challenged with 15 nm Au nanospheres either coated by a citrate layer or passivated by a protein corona. The effect of Au...
GOLD NANOPARTICLES | PHYSICS, APPLIED | PARTICLES | MECHANISM | MATERIALS SCIENCE, MULTIDISCIPLINARY | SIZE | PENETRATION | NANOSCIENCE & NANOTECHNOLOGY | MODEL | CHEMISTRY, MULTIDISCIPLINARY | CELLULAR UPTAKE | ADHESION | PROTEIN CORONA | Gold - chemistry | Models, Chemical | Metal Nanoparticles - chemistry | Models, Molecular | Materials Testing | Lipid Bilayers - chemistry | Diffusion | Metal Nanoparticles - ultrastructure
GOLD NANOPARTICLES | PHYSICS, APPLIED | PARTICLES | MECHANISM | MATERIALS SCIENCE, MULTIDISCIPLINARY | SIZE | PENETRATION | NANOSCIENCE & NANOTECHNOLOGY | MODEL | CHEMISTRY, MULTIDISCIPLINARY | CELLULAR UPTAKE | ADHESION | PROTEIN CORONA | Gold - chemistry | Models, Chemical | Metal Nanoparticles - chemistry | Models, Molecular | Materials Testing | Lipid Bilayers - chemistry | Diffusion | Metal Nanoparticles - ultrastructure
Journal Article
ACS Applied Materials & Interfaces, ISSN 1944-8244, 06/2016, Volume 8, Issue 24, pp. 14944 - 14951
SiO2/TiO2 microbeads (T-rex) are promising materials for plasmon-free surface-enhanced Raman scattering (SERS), offering several key advantages in...
plasmon-free SERS | T-rex | cavitands | core/shell colloids | all-dielectric beads | methylated lysine | RECOGNITION | MATERIALS SCIENCE, MULTIDISCIPLINARY | ENVIRONMENT | N-epsilon-methylated lysine | SENSITIVITY | NANOSCIENCE & NANOTECHNOLOGY | SCATTERING ENHANCERS | NANOANTENNAS | NANOPARTICLES | SPECTROSCOPY | SERS | FUNCTIONALIZED SILICON SURFACE | TIO2 | Spectrum Analysis, Raman | Lysine - chemistry | Methylation | Silicon Dioxide | Resorcinols | Ethers, Cyclic
plasmon-free SERS | T-rex | cavitands | core/shell colloids | all-dielectric beads | methylated lysine | RECOGNITION | MATERIALS SCIENCE, MULTIDISCIPLINARY | ENVIRONMENT | N-epsilon-methylated lysine | SENSITIVITY | NANOSCIENCE & NANOTECHNOLOGY | SCATTERING ENHANCERS | NANOANTENNAS | NANOPARTICLES | SPECTROSCOPY | SERS | FUNCTIONALIZED SILICON SURFACE | TIO2 | Spectrum Analysis, Raman | Lysine - chemistry | Methylation | Silicon Dioxide | Resorcinols | Ethers, Cyclic
Journal Article
Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, 09/2016, Volume 145, pp. 520 - 525
We report on the first quantitative picture on how iron loading inside ferritin molecules occurs when they are self-assembled onto solid surfaces. Recombinant...
Iron loading | Microcantilevers | Nanomechanics | Ferritin | Surface | STORAGE | MATERIALS SCIENCE, BIOMATERIALS | PROTEIN | CONTACT-ANGLE | AFM | CHEMISTRY, PHYSICAL | BIOPHYSICS | BIOSENSORS | ELECTRODE | FORCES | Humans | Microscopy, Atomic Force | Signal Processing, Computer-Assisted | Ferric Compounds - chemistry | Iron - metabolism | Apoferritins - metabolism | Protein Subunits - metabolism | Thin films | Dielectric films | Surface energy | Surface chemistry | Transformations | Iron | Nanostructure | Nanotechnology | Recombinant
Iron loading | Microcantilevers | Nanomechanics | Ferritin | Surface | STORAGE | MATERIALS SCIENCE, BIOMATERIALS | PROTEIN | CONTACT-ANGLE | AFM | CHEMISTRY, PHYSICAL | BIOPHYSICS | BIOSENSORS | ELECTRODE | FORCES | Humans | Microscopy, Atomic Force | Signal Processing, Computer-Assisted | Ferric Compounds - chemistry | Iron - metabolism | Apoferritins - metabolism | Protein Subunits - metabolism | Thin films | Dielectric films | Surface energy | Surface chemistry | Transformations | Iron | Nanostructure | Nanotechnology | Recombinant
Journal Article
Acta Materialia, ISSN 1359-6454, 01/2006, Volume 54, Issue 7, pp. 1843 - 1849
A microwave (MW) field can induce in a dielectric material an oscillatory polarization. By this mechanism part of the energy carried by the waves is converted...
Heat conduction | Conduction | Polarization | Heating | Mathematical analysis | Nonequilibrium thermodynamics | Heat transfer | Microwaves
Heat conduction | Conduction | Polarization | Heating | Mathematical analysis | Nonequilibrium thermodynamics | Heat transfer | Microwaves
Journal Article
2013, Methods in molecular biology, ISBN 9781627034616, Volume 1025
Web Resource
2013, Methods in molecular biology, ISBN 9781627034616, Volume 1025
Web Resource
Biochemistry, ISSN 0006-2960, 12/2017, Volume 56, Issue 48, pp. 6401 - 6408
Sialidases are glycohydrolases that remove terminal sialic acid residues from oligosaccharides, glycolipids, and glycoproteins. The plasma membrane-associated...
FREE LIGHT-CHAINS | FORCE SPECTROSCOPY | EXTRACELLULAR VESICLES | BIOGENESIS | CRYSTAL-STRUCTURE | BIOCHEMISTRY & MOLECULAR BIOLOGY | MOLECULAR-CLONING | GANGLIOSIDE SIALIDASE | INFLAMMATORY CYTOKINES | HUMAN-SALIVA | EXPRESSION | Cell Membrane - enzymology | Exosomes - physiology | Humans | Neuraminidase - genetics | Neuraminidase - metabolism | Gene Expression Regulation, Enzymologic - physiology | HeLa Cells | Extracellular fluid | Cell membranes | Biochemistry | Research
FREE LIGHT-CHAINS | FORCE SPECTROSCOPY | EXTRACELLULAR VESICLES | BIOGENESIS | CRYSTAL-STRUCTURE | BIOCHEMISTRY & MOLECULAR BIOLOGY | MOLECULAR-CLONING | GANGLIOSIDE SIALIDASE | INFLAMMATORY CYTOKINES | HUMAN-SALIVA | EXPRESSION | Cell Membrane - enzymology | Exosomes - physiology | Humans | Neuraminidase - genetics | Neuraminidase - metabolism | Gene Expression Regulation, Enzymologic - physiology | HeLa Cells | Extracellular fluid | Cell membranes | Biochemistry | Research
Journal Article
Journal of Molecular Recognition, ISSN 0952-3499, 03/2011, Volume 24, Issue 2, pp. 182 - 187
The origin of the difference between the equilibrium (affinity) constants of ligand–receptor binding in bulk solution and at a solid‐solution interface is...
surface bioassays | biosensors | DNA hybridization | surface molecular recognition | affinity | PROTEIN | NANOMECHANICAL DETECTION | BIOCHEMISTRY & MOLECULAR BIOLOGY | TENSION | MODEL | ADSORPTION | THERMODYNAMICS | ISOTHERMS | BIOPHYSICS | FORCES | BINDING | Receptors, Cell Surface - metabolism | Ligands | DNA - chemistry | Nucleic Acid Hybridization | Kinetics | Solutions - chemistry
surface bioassays | biosensors | DNA hybridization | surface molecular recognition | affinity | PROTEIN | NANOMECHANICAL DETECTION | BIOCHEMISTRY & MOLECULAR BIOLOGY | TENSION | MODEL | ADSORPTION | THERMODYNAMICS | ISOTHERMS | BIOPHYSICS | FORCES | BINDING | Receptors, Cell Surface - metabolism | Ligands | DNA - chemistry | Nucleic Acid Hybridization | Kinetics | Solutions - chemistry
Journal Article
Journal of Colloid And Interface Science, ISSN 0021-9797, 04/2018, Volume 516, pp. 284 - 294
Understanding the interaction between nanomaterials and biological interfaces is a key unmet goal that still hampers clinical translation of nanomedicine. Here...
Giant unilamellar vesicles | Gold nanorods | Nanomedicine | Gold nanoparticles | Biomimetic membranes | Supported lipid bilayers | INTERNALIZATION | VESICLES | CHEMISTRY, PHYSICAL | Nanoparticles | Thermodynamics | Biomimetics | Macrophages | Analysis
Giant unilamellar vesicles | Gold nanorods | Nanomedicine | Gold nanoparticles | Biomimetic membranes | Supported lipid bilayers | INTERNALIZATION | VESICLES | CHEMISTRY, PHYSICAL | Nanoparticles | Thermodynamics | Biomimetics | Macrophages | Analysis
Journal Article
2013, 2013, Methods in Molecular Biology, ISBN 1627034617, Volume 1025
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