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EMBO Journal, ISSN 0261-4189, 08/2015, Volume 34, Issue 15, p. 1987
  Strict temporal control of cell cycle gene expression is essential for all eukaryotes including animals and plants. DREAM complexes have been identified in... 
Botany | Cell cycle | Gene expression
Journal Article
Cancer treatment and research, ISSN 0927-3042, 2019, Volume 177, pp. 211 - 229
Merkel cell carcinoma (MCC) is a highly aggressive, primary neuroendocrine cancer of the skin. The majority of MCC cases are associated with the recently... 
Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 01/2019, Volume 116, Issue 3, pp. 1027 - 1032
Merkel cell polyomavirus (MCV) contributes to approximately 80% of all Merkel cell carcinomas (MCCs), a highly aggressive neuroendocrine carcinoma of the skin.... 
MDM2–MDM4 | Lenalidomide | Merkel cell carcinoma | Casein kinase 1 alpha | P53 | UBIQUITINATION | casein kinase 1 alpha | POLYOMAVIRUS | MULTIDISCIPLINARY SCIENCES | GROWTH | DEGRADATION | MDM2-MDM4 | lenalidomide | p53 | T-ANTIGEN | Tumor Virus Infections - genetics | Proto-Oncogene Proteins c-mdm2 - genetics | Humans | Polyomavirus Infections - metabolism | Tumor Suppressor Protein p53 - genetics | DNA-Binding Proteins - metabolism | Retinoblastoma Binding Proteins - genetics | Nuclear Proteins - genetics | Retinoblastoma Binding Proteins - metabolism | DNA Helicases - genetics | Proto-Oncogene Proteins c-mdm2 - antagonists & inhibitors | Proto-Oncogene Proteins c-mdm2 - metabolism | Tumor Virus Infections - pathology | Proto-Oncogene Proteins - metabolism | Proto-Oncogene Proteins - antagonists & inhibitors | Tumor Virus Infections - metabolism | Carcinoma, Merkel Cell - genetics | Tumor Suppressor Protein p53 - metabolism | Ubiquitin-Protein Ligases - metabolism | Merkel cell polyomavirus - metabolism | Nuclear Proteins - metabolism | Proto-Oncogene Proteins - genetics | DNA-Binding Proteins - genetics | Polyomavirus Infections - pathology | DNA Helicases - metabolism | Nuclear Proteins - antagonists & inhibitors | Polyomavirus Infections - genetics | Carcinoma, Merkel Cell - metabolism | Merkel cell polyomavirus - genetics | Carcinoma, Merkel Cell - virology | Ubiquitin-Protein Ligases - genetics | Carcinoma, Merkel Cell - pathology | Physiological aspects | Care and treatment | Tumor proteins | Oncogenes | Chromatin | Carcinoma | p53 Protein | Antigen T (large) | Genes | Xenotransplantation | Viruses | Homology | Activation | Myc protein | Gene sequencing | Proteins | Rodents | Xenografts | MDM2 protein | Ribonucleic acid--RNA | Gene expression | Depletion | Inhibitors | Cell lines | Skin | Mutation | Cancer | Tumors | Apoptosis | Index Medicus | Biological Sciences
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 07/2019, Issue 7
Dounce homogenization in combination with hypotonic buffers facilitates the lysis of adherent and suspension cells. Addition of hypotonic buffers results in... 
Membranes | Chromatin | Transcription factors | Salts | Immunoprecipitation | Incubation | Homogenization | Cell membranes | Cell membranes (biology) | Nuclei | Proteins | Lysis | Histones | Dialysis | Nuclei (cytology) | Buffers | Cytoplasm
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 03/2019, Volume 2019, Issue 3, pp. 224 - 226
Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs). An efficient way of generating antibodies is to introduce antigens of interest into... 
Antigens | Antigen presentation | Immune response | Dendritic cells | Transgenic | Antibodies | Lymphocytes T | Immunity | Immune systems | Proteins | Antigen-presenting cells | Immune system | Apoptosis
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 02/2019, Volume 2019, Issue 2, pp. 147 - 153
The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely... 
Elution | Immunoprecipitation | Purification | Yeast | Peptides | Protein purification | Antibodies | Mass spectroscopy | Proteins | Hemagglutinins | Affinity | Mass spectrometry | Binding sites | Tags
Journal Article
Cell Reports, ISSN 2211-1247, 09/2016, Volume 16, Issue 12, pp. 3359 - 3372
The anaphase promoting complex/cyclosome (APC/C) is an ubiquitin ligase and core component of the cell-cycle oscillator. During G1 phase, APC/C binds to its... 
MITOSIS | ANAPHASE-PROMOTING COMPLEX | GENES | SPINDLE ASSEMBLY CHECKPOINT | CELL-CYCLE PROGRESSION | IDENTIFICATION | UBIQUITIN LIGASES | DEPENDENT KINASES | CDH1 DEGRADATION | DNA-REPLICATION | CELL BIOLOGY
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 12/2018, Volume 2018, Issue 12, pp. 1012 - 1014
Baculovirus-produced recombinant proteins circumvent many of the issues that limit bacterial protein production. Baculoviruses contain double-stranded,... 
Cell culture | Phosphorylation | Recombination | Polyhedra | Viruses | Infections | Baculovirus | Plant cells | Proteins | Suspension culture | Inclusion bodies | Life cycle engineering | Deoxyribonucleic acid--DNA | Recombinant | Antigens | Cloning vectors | Cloning | Glycoprotein | Glycosylation | Mammals | Gene expression | Uncoating | Insects | Insect cells | Lysis | Life cycles
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 09/2018, Volume 2018, Issue 9, pp. 744 - 748
In this protocol, cells are cultured in the presence of [ P]orthophosphate used by the cell to phosphorylate proteins. Cells must be maintained at 37°C at all... 
Proteins | Antigens | Lysates | Autoradiography | Cellulose esters | Fluoride | Immunoprecipitation | Orthophosphate | Cellulose nitrate | Polyvinylidene fluorides | Metabolism | Western blotting
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 09/2018, Volume 2018, Issue 9, pp. 738 - 743
Pulse-chase labeling of antigens with [ S]methionine is used to determine the relative half-life of a protein. In this protocol, intracellular unlabeled... 
Adherent cells | Cell culture | Antigens | Starvation | Cellulose esters | Immunoprecipitation | Protein purification | Methionine | Labelling | Radioactive half-life | Freezing | Proteins | Lysates | Autoradiography | Fluoride | Polypropylene | Lysis | Cellulose nitrate | Polyvinylidene fluorides | Chemical synthesis | Target detection
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 10/2017, Volume 2017, Issue 10, pp. 842 - 847
Metabolic labeling of antigens can be achieved by supplementation of the cell culture medium with radioactive amino acid precursors such as [ S]methionine... 
Isotope Labeling - methods | Antigens - metabolism | Sulfur Radioisotopes - metabolism | Culture Media - chemistry | Methionine - metabolism | Cell Culture Techniques - methods
Journal Article
Nature Reviews Cancer, ISSN 1474-175X, 10/2013, Volume 13, Issue 10, p. 752
Journal Article
NATURE REVIEWS CANCER, ISSN 1474-175X, 10/2013, Volume 13, Issue 10, pp. 752 - 752
Journal Article
Cold Spring Harbor protocols, ISSN 1940-3402, 10/2018, Volume 2018, Issue 10, pp. 810 - 811
Haptens, which are small antigens such as peptides and drug compounds, are very weakly or nonimmunogenic by themselves and require the assistance of carrier... 
Antigens | Rabbits | Conjugates | Immune response | Peptides | Antibodies | Crosslinking | Mollusks | Haptens | Lymphocytes T | Epitopes | Mammals | Cell surface | Proteins | Immune systems | T-cell receptor | Organic chemistry | Major histocompatibility complex | Lymphocytes B | Binding sites
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 05/2018, Volume 2018, Issue 5, pp. 400 - 401
Many molecules can be made more immunogenic by denaturation. This treatment will change the structure of many compounds, particularly proteins, and expose new... 
Antigens | Heat treatment | Protein denaturation | Immunoprecipitation | Sodium lauryl sulfate | Denaturation | Antibodies | Epitopes | Antibody response | Proteins | Sodium dodecyl sulfate | Sodium | Immunogenicity | Translations
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 05/2018, Volume 2018, Issue 5, pp. 398 - 399
Many compounds on their own do not have all of the properties needed to induce a strong antibody response. However, small changes in the structure of an... 
Binding | Coupling (molecular) | Antigens | Arsenates | Immunogenicity | Lymphocytes B | Antisera | Lymphocytes T | Epitopes | Antibody response
Journal Article
Clinical Cancer Research, ISSN 1078-0432, 08/2015, Volume 21, Issue 16 Supplement, pp. POSTER-BIOL-1324A - POSTER-BIOL-1324A
Journal Article
Genes & Development, ISSN 0890-9369, 03/2012, Volume 26, Issue 5, p. 474
Journal Article
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