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Journal Article
Molecular Therapy, ISSN 1525-0016, 08/2018, Volume 26, Issue 8, pp. 2070 - 2076
Cpf1 has been harnessed as a tool for genome manipulation in various species because of its simplicity and high efficiency. Our recent study demonstrated that... 
FnCpf1 | multiplex genome editing | crRNA | MEDICINE, RESEARCH & EXPERIMENTAL | SPECIFICITIES | RNA | HUMAN-CELLS | CPF1 | CRISPR-CAS SYSTEM | TOOLKIT | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GENETICS & HEREDITY | PLANTS | GENERATION | KNOCKOUT | MICE | CRISPR | Flow cytometry | Nucleotide sequence | Plasmids | Efficiency | Genes | Software | Genomes | Mutation | Gene therapy | Ribonucleic acid--RNA | Original
Journal Article
Molecular Therapy, ISSN 1525-0016, 11/2018, Volume 26, Issue 11, pp. 2650 - 2657
FnCpf1-mediated genome-editing technologies have enabled a broad range of research and medical applications. Recently, we reported that FnCpf1 possesses... 
FnCpf1 | genome editing | direct repeat | MEDICINE, RESEARCH & EXPERIMENTAL | SPECIFICITIES | ENDONUCLEASE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | CPF1 | GENETICS & HEREDITY | CRISPR-CAS9 | MICE | NUCLEASES | Proteins | CRISPR | Fidelity | Engineering | Nucleotide sequence | Efficiency | Genomes | Mutation | Deoxyribonucleic acid--DNA | Index Medicus
Journal Article
by 盖爽
文教资料, ISSN 1004-8359, 2010, Issue 13, pp. 59 - 60
文章作者对一位年轻教师讲解的世界名著——莫泊桑的《项链》进行了实录,认真分析了其适时有效的课堂提问和开放性的思路引导.并指出课堂中出现的一些细节性问题.最后点评,认为课堂教学总体上比较成功,反映了教师扎实的教学基本功和良好的文学底蕴.值得同仁好好学习。 
点评 | 《项链》教学实录
Journal Article
Journal Article
男生女生:银版, ISSN 1009-5594, 2016, Issue 6, pp. 93 - 93
陈超是一个小偷。最近的几起盗窃案都是他做的,每次警察来的时候他都会提前逃跑,所以根本抓不到他,这也是他最得意的地方。这次他又有目标了,在街上看到一个穿着华丽的妇人,脖子上挂着一串价值连城的珍珠项链。陈超一路尾随,摸清了那妇人的住处后,决定今晚就动手。 
珍珠项链 | 盗窃案 | 穿着 | 陈超
Journal Article
Scientific reports, ISSN 2045-2322, 2014, Volume 4, Issue 1, pp. 6936 - 6936
Journal Article
Molecular Therapy - Nucleic Acids, ISSN 2162-2531, 2016, Volume 5, Issue 11, pp. e393 - e393
Clustered interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome engineering technologies are sparking a new... 
NRL | CRISPR/Cas9 | homologous recombination | AAV | next generation sequencing | human embryonic stem cells | MEDICINE, RESEARCH & EXPERIMENTAL | CAS9 NUCLEASES | SPECIFICITY | HUMAN IPSCS | IDENTIFICATION | TRANSPLANTATION | ADENOASSOCIATED VIRUS VECTORS | DIFFERENTIATION | PHOTORECEPTOR DEGENERATION | EFFICIENT | Proteins | Genomes | Mutation | Deoxyribonucleic acid--DNA | CRISPR | Cas9 | Original
Journal Article
Molecular Therapy - Nucleic Acids, ISSN 2162-2531, 06/2017, Volume 7, Issue C, pp. 378 - 386
Cre- , as one of the site-specific genetic manipulation tools, offers a method to study the spatial and temporal regulation of gene expression/inactivation in... 
loxP | NHEJ | CRISPR/Cas9 | gene editing | AAVS1 | MEDICINE, RESEARCH & EXPERIMENTAL | CAS9 NUCLEASES | CRISPR-CAS9 NUCLEASES | EMBRYONIC STEM-CELLS | IN-VIVO | CRE | MOUSE | GENOME-WIDE | GENERATION | KNOCKOUT | MAMMALIAN-CELLS
Journal Article
Biotechnology Journal, ISSN 1860-6768, 03/2018, Volume 13, Issue 3, pp. e1800080 - n/a
Journal Article
Frontiers in Molecular Neuroscience, ISSN 1662-5099, 01/2018, Volume 10, pp. 453 - 453
X-linked juvenile retinoschisis (XLRS) is a retinal disease caused by mutations in the gene encoding retinoschisin (RS1), which leads to a significant... 
Retina | Mice | X-linked juvenile retinoschisis | Genome editing technologies | Knock-in | Disease model | CELLS | SAFETY | knock-in | genome editing technologies | MECHANISMS | PATHOLOGY | mice | NEUROSCIENCES | VECTOR | retina | DISEASE | GENE-THERAPY | RS1H | disease model
Journal Article
开封教育学院学报, ISSN 1008-9640, 2013, Issue 8, pp. 18 - 19
Journal Article
by 葛勇
中国统一战线, ISSN 1005-5819, 2012, Issue 10, pp. 76 - 76
... 
民族 | 活着 | 土地
Journal Article
Biotechnology Journal, ISSN 1860-6768, 04/2018, Volume 13, Issue 4, pp. 1700561 - n/a
Currently, very few studies have attempted to study the PAM sequence of CRISPR/Cas9 directly in human cells. To address this issue, the authors develop a dual... 
FnCpf1 | dual fluorescence reporter | activity | CRISPR/Cas9 | PAM | CRISPR | BIOCHEMICAL RESEARCH METHODS | CRISPR-CAS9 | STAPHYLOCOCCUS-AUREUS CAS9 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | RNA-GUIDED ENDONUCLEASE | SYSTEMS | Cas9 | PROTEINS | TARGET DNA RECOGNITION | Fluorescence | Nucleases | Chromosomes | Analysis | Genomics | Cells | Index Medicus
Journal Article
Journal of Genetics and Genomics, ISSN 1673-8527, 06/2018, Volume 45, Issue 6, pp. 329 - 332
Journal Article
20.