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Methods in Molecular Biology, ISSN 1064-3745, 2015, Volume 1313, pp. 41 - 59
Production of the germline-competent chimeras using genetically modified ES cell lines is an essential step in the establishment of novel mouse models. In... 
Aggregation | Embryo | Mouse | Tetraploid | Embryonic stem (ES) cells | Cell fusion | Chimera | Embryonic stem (es) cells | Tetraploidy | Cell Fusion | Animals | Embryo Transfer | Embryo, Mammalian | Cell Aggregation | Mice | Cell Culture Techniques | Embryonic Stem Cells | Genetic Complementation Test | Index Medicus
Journal Article
Journal Article
Cold Spring Harbor protocols, ISSN 1940-3402, 05/2019, Volume 2019, Issue 5, p. pdb.prot092775
Fluorescent proteins (FPs) are popular reporters available for gene expression detection and determination of cellular identities in the mouse. This protocol... 
Proteins | Fluorescence | Gene expression | Green fluorescent protein
Journal Article
Cold Spring Harbor protocols, ISSN 1940-3402, 05/2019, Volume 2019, Issue 5, p. pdb.prot092767
Frequently, there is a need for fluorescent protein detection in mouse cell cultures, including embryonic stem cells or their differentiated derivatives,... 
Proteins | Microscopy | Transformed cells | Embryo cells | Green fluorescent protein | Stem cells | Fluorescence | Stem cell transplantation | Embryos
Journal Article
Cold Spring Harbor protocols, ISSN 1940-3402, 04/2019, Volume 2019, Issue 4, p. pdb.prot092692
DNA samples are prepared from mouse tail tips by Proteinase K digestion and subsequently extracted. The resulting preparation is suitable for use in Southern... 
Polymerase chain reaction | Endopeptidase K | Southern blotting | Proteinase | Deoxyribonucleic acid--DNA
Journal Article
Cold Spring Harbor protocols, ISSN 1940-3402, 04/2019, Volume 2019, Issue 4, p. pdb.prot092700
A simple cell or tissue lysate can provide a sufficient quality and amount of template DNA for polymerase chain reaction (PCR). In this protocol, a small piece... 
Polymerase chain reaction | Sodium | Tissues | Deoxyribonucleic acid--DNA | Sodium hydroxide
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 01/2019, Volume 2019, Issue 1, pp. 85 - 86
Often, genotyping of mouse embryos is required, and a small part, such as the yolk sac, can be used for this purpose. Here, DNA samples are prepared from... 
Proteinase | Gestation | Tissues | Embryos | Weight | Polymerase chain reaction | Yolk sac | Endopeptidase K | Genotyping | Fish | Genetic testing | Deoxyribonucleic acid--DNA | Yolk
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 12/2018, Volume 2018, Issue 12, pp. 967 - 970
To obtain ubiquitous or simply widespread transgene expression from a single stable integrant transgene is quite challenging because the random genomic... 
Integration | Rodents | Translation initiation | Doxycycline | Transgenic | Transgenes | Gene expression | Loci | Gene fusion
Journal Article
Methods in molecular biology (Clifton, N.J.), 2020, Volume 2066, pp. 89 - 100
Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell... 
Index Medicus
Journal Article
PLoS Genetics, ISSN 1553-7390, 08/2018, Volume 14, Issue 8, pp. e1007587 - e1007587
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 01/2018, Volume 2018, Issue 1, pp. 24 - 27
For experiments that require large numbers of preimplantation mouse embryos, such as microinjection of zygotes, gonadotropins are administered to females... 
Gonadotropins - administration & dosage | Female | Mice | Animals | Superovulation | Zygotes | Follicle-stimulating hormone | Chorionic gonadotropin | Microinjection | Luteinizing hormone | Pituitary (anterior) | Gonadotropins | Females | Ovulation | Embryos | Oocytes
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 03/2018, Volume 2018, Issue 3, pp. 171 - 172
Fluorescent protein (FP)-expressing transgenic mice are powerful genetic resources for marking both live and fixed cells and tissues. Expression in live... 
Proteins | Visualization | Rodents | Genetic resources | Transgenic mice | Fluorescence | Embryos
Journal Article
Methods in molecular biology (Clifton, N.J.), 2020, Volume 2066, pp. 59 - 82
Genetic modification of mouse embryonic stem (ES) cells is a powerful technology that enabled the generation of a plethora of mutant mouse lines to study gene... 
Index Medicus
Journal Article
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