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1. Immobilization of enzymes and cells
by Guisan, Jose M
2006, 2nd ed., Methods in biotechnology, ISBN 9781588292902, Volume 22., xiv, 449
Biotechnology | Immobilized enzymes | Immobilized cells | Chemistry
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2. Full Text Stabilization of immobilized lipases by intense intramolecular cross-linking of their surfaces by using aldehyde-dextran polymers
by Orrego, Alejandro H and Ghobadi, Rohollah and Moreno-Perez, Sonia and Mendoza, Adriana Jaime and Fernandez-Lorente, Gloria and Guisan, Jose M and Rocha-Martin, Javier
International Journal of Molecular Sciences, ISSN 1661-6596, 02/2018, Volume 19, Issue 2, p. 553
Immobilized enzymes have a very large region that is not in contact with the support surface and this region could be the target of new stabilization... 
Dextran | Chemical amination | Enzyme cross-linking | Enzyme stabilization | Stabilizing polymers | Aldehyde | Lipase immobilization | enzyme stabilization | stabilizing polymers | STABILITY | BIOCHEMISTRY & MOLECULAR BIOLOGY | CHEMICAL-MODIFICATION | lipase immobilization | CHEMISTRY, MULTIDISCIPLINARY | enzyme cross-linking | GALACTOSIDASE | THERMOMYCES-LANUGINOSUS | INTERFACIAL ADSORPTION | ENZYME | HYDROPHOBIC SUPPORTS | chemical amination | HYPERACTIVATION | CANDIDA-ANTARCTICA | GLYOXYL AGAROSE BEADS | aldehyde-dextran | Enzymes | Conjugates | Sodium lauryl sulfate | Gel electrophoresis | Stabilization | Crosslinking | Amino acids | Hydrophobicity | Sodium dodecyl sulfate | Immobilized enzymes | Catalysis | Polymers | Amino groups | aldehyde–dextran
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3. Full Text Improving the thermostability and optimal temperature of a lipase from the hyperthermophilic archaeon pyrococcus furiosus by covalent immobilization
by Branco, Roberta V and Gutarra, Melissa L. E and Guisan, Jose M and Freire, Denise M. G and Almeida, Rodrigo V and Palomo, Jose M
BioMed Research International, ISSN 2314-6133, 2015, Volume 2015, p. 250532
A recombinant thermostable lipase (Pf2001 Delta 60) from the hyperthermophilic Archaeon Pyrococcus furiosus (PFUL) was immobilized by hydrophobic interaction... 
MEDICINE, RESEARCH & EXPERIMENTAL | INTERFACIAL ADSORPTION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | STABILIZATION | PURIFICATION | RESOLUTION | SUPPORTS | CANDIDA-RUGOSA LIPASE | HYPERACTIVATION | COMPARATIVE PERFORMANCE | DERIVATIVES | ESTERASE | Enzymes, Immobilized - chemistry | Archaea - metabolism | Temperature | Pyrococcus furiosus - metabolism | Glyoxylates - chemistry | Sepharose - chemistry | Stereoisomerism | Enzyme Stability | Hydrophobic and Hydrophilic Interactions | Lipase - chemistry | Bacterial Proteins | Hydrogen-Ion Concentration | Thermal properties | Physiological aspects | Pyroccus furiosus | Lipase | Chemical bonds | Enzymes | Acids | Biocatalysts
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4. Full Text Oxidation of phenolic compounds catalyzed by immobilized multi-enzyme systems with integrated hydrogen peroxide production
by Rocha-Martin, Javier and Velasco-Lozano, Susana and Guisán, José M and López-Gallego, Fernando
Green Chemistry, ISSN 1463-9262, 01/2014, Volume 16, Issue 1, pp. 303 - 311
Suicide inactivation of peroxidases by hydrogen peroxide is the major deterrent to using such biocatalysts in oxidative processes. This has been successfully... 
REMOVAL | ENZYME | WASTE-WATER | ACTIVATED SUPPORTS | GLUCOSE-OXIDASE | STABILIZATION | GLYOXYL-AGAROSE | POLYMERIZATION | HORSERADISH-PEROXIDASE | VALUABLE TOOL | CHEMISTRY, MULTIDISCIPLINARY | Enzymes | Formic acid | Cascades | Phenols | Peroxidase | Oxidation | Derivatives | Beads
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5. Full Text Modulation of the regioselectivity of Thermomyces lanuginosus lipase via biocatalyst engineering for the Ethanolysis of oil in fully anhydrous medium
by Abreu Silveira, Erick and Moreno-Perez, Sonia and Basso, Alessandra and Serban, Simona and Pestana Mamede, Rita and Tardioli, Paulo W and Sanchez Farinas, Cristiane and Rocha-Martin, Javier and Fernandez-Lorente, Gloria and Guisan, Jose M
BMC Biotechnology, ISSN 1472-6750, 12/2017, Volume 17, Issue 1, pp. 88 - 13
Background: Enzymatic ethanolysis of oils (for example, high oleic sunflower oil containing 90% of oleic acid) may yield two different reaction products... 
2-monoolein | Modulation of TLL regioselectivity | Ethyl oleate | Lipase immobilization | INDUSTRIAL APPLICATIONS | CATALYZED TRANSESTERIFICATION | ENZYME IMMOBILIZATION | ETHYL OLEATE SYNTHESIS | HETEROLOGOUS RHIZOPUS-ORYZAE | INTERFACIAL ADSORPTION | HYDROPHOBIC SUPPORTS | BIODIESEL PRODUCTION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | ACYL MIGRATION | SELECTIVE ETHANOLYSIS | Fungal Proteins - chemistry | Metabolic Engineering | Enzymes, Immobilized - chemistry | Enzymes, Immobilized - metabolism | Oleic Acid - metabolism | Ethanol - metabolism | Stereoisomerism | Lipase - metabolism | Lipase - chemistry | Oleic Acids - metabolism | Adsorption | Bioreactors | Eurotiales - enzymology | Fungal Proteins - metabolism | Lipase | Analysis
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6. Covalent immobilization-stabilization of ß-1,4-endoxylanases from Trichoderma reesei: Production of xylooligosaccharides
by Sandro Martins de Oliveira and Sonia Moreno-Perez and César Rafael Fanchini Terrasan and Maria Romero-Fernández and Marcelo Fernandes Vieira and Jose M Guisan and Javier Rocha-Martin
Process Biochemistry, ISSN 1359-5113, 01/2018, Volume 64, p. 170
The production of xylooligosaccharides (XOS) was evaluated using immobilized and stabilized biocatalysts of a commercial enzymatic cocktail, Bioxilanase L PLUS... 
Polyethyleneimine | Enzymes | Trichoderma | Immobilization | Xylan | Radioactive half-life | Biochemistry | Biocatalysts | pH effects | Beads | Conversion | Trichoderma reesei
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7. Immobilization of Enzymes and Cells
by Guisan, Jose M. Editor
08/2013, 3rd ed., Methods in Molecular Biology Ser., ISBN 9781627035491, Volume 1051
Annotation For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used... 
Enzymes | Biotechnology | Science | Cells
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8. Full Text One-step Synthesis of -Keto Acids from Racemic Amino Acids by A Versatile Immobilized Multienzyme Cell-free System
by Orrego, AH and Lopez-Gallego, F and Espaillat, A and Cava, F and Guisan, JM and Rocha-Martin, J
CHEMCATCHEM, ISSN 1867-3880, 07/2018, Volume 10, Issue 14, pp. 3002 - 3011
The elevated value of -keto acids has pushed scientists to explore more efficient and less expensive alternatives for their synthesis. In this work, an... 
CANCER-CELLS | SELECTIVE OXIDATION | immobilization | ACTIVATED SUPPORTS | PLP-dependent enzymes | ALPHA-KETOGLUTARIC ACID | CHEMISTRY, PHYSICAL | CHITOSAN SUPPORT | PROTEIN IMMOBILIZATION | biocatalysis | alpha-keto acids | L-LEUCINE | ENZYMES | PYRIDOXAL-PHOSPHATE | dynamic kinetic resolution | CORYNEBACTERIUM-GLUTAMICUM | Oxidases | Amino acids | Immobilized enzymes | Catalysis | Enantiomers
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9. Immobilization of enzymes and cells
by Guisan, Jose M
2013, Third Edition., Methods in molecular biology, ISBN 9781627035491, Volume 1051.
Biotechnology | methods | Cells, Immobilized | Enzymes, Immobilized
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10. Immobilization of enzymes and cells
by Guisan, Jose M
2013, Third Edition., Methods in molecular biology, ISBN 9781627035491, Volume 1051.
Biotechnology | Immobilized enzymes | methods | Cells, Immobilized | Enzymes, Immobilized
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11. Full Text Covalent immobilization-stabilization of [beta]-1,4-endoxylanases from Trichoderma reesei: Production of xylooligosaccharides
by de Oliveira, Sandro Martins and Moreno-Perez, Sonia and Terrasan, Cesar Rafael Fanchini and Romero-Fernandez, Maria and Vieira, Marcelo Fernandes and Guisan, Jose M and Rocha-Martin, Javier
Process Biochemistry, ISSN 1359-5113, 01/2018, Volume 64, p. 170
To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1016/j.procbio.2017.09.018 The... 
Hydrolysis | Enzymes | Surface active agents | Catalysis | Aldehydes | Production processes
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12. Full Text Preparation of a robust immobilized biocatalyst of [beta]-1,4-endoxylanase by surface coating with polymers for production of xylooligosaccharides from different xylan sources
by Romero-Fernandez, Maria and Moreno-Perez, Sonia and Martins de Oliveira, Sandro and Santamaria, Ramon I and Guisan, Jose M and Rocha-Martin, Javier
New Biotechnology, ISSN 1871-6784, 09/2018, Volume 44, p. 50
Xylooligosaccharides display interesting prebiotic effects on human health. The endoxylanase Xys1[DELTA], from Streptomyces halstedii JM8, was immobilized and... 
Dextran | Enzymes | Nitriles | Polymer industry | Catalysis | Coatings | Wheat
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13. Preparation of a robust immobilized biocatalyst of β-1,4-endoxylanase by surface coating with polymers for production of xylooligosaccharides from different xylan sources
by Maria Romero-Fernández and Sonia Moreno-Perez and Sandro Martins de Oliveira and Ramón I Santamaría and Jose M Guisan and Javier Rocha-Martin
New Biotechnology, ISSN 1871-6784, 09/2018, Volume 44, p. 50
Xylooligosaccharides display interesting prebiotic effects on human health. The endoxylanase Xys1Δ, from Streptomyces halstedii JM8, was immobilized and... 
Polyethyleneimine | Cellulose | Xylan | Catalytic activity | Biocatalysts | Coatings | Straw | Dextran | Multilayers | Catalysis | Coating | Polymers | Beads | Protective coatings | Wheat | Sugar
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14. Full Text Improvement of enzyme activity, stability and selectivity via immobilization techniques
by Mateo, Cesar and Palomo, Jose M and Fernandez-Lorente, Gloria and Guisan, Jose M and Fernandez-Lafuente, Roberto
Enzyme and Microbial Technology, ISSN 0141-0229, 2007, Volume 40, Issue 6, pp. 1451 - 1463
In spite of their excellent catalytic properties, enzyme properties have to be usually improved before their implementation at industrial scale (where many... 
Immobilization | Reduced inhibition | Improved specificity | Stabilization | Improved selectivity | ANTARCTICA-B LIPASE | PENICILLIN-G ACYLASE | immobilization | MULTIPOINT COVALENT ATTACHMENT | stabilization | improved specificity | MULTIMERIC BETA-GALACTOSIDASE | DIRECTED EVOLUTION | FORMATE DEHYDROGENASE | GLUTARALDEHYDE CROSS-LINKING | reduced inhibition | GLYOXYL-AGAROSE SUPPORTS | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | CANDIDA-RUGOSA LIPASE | AMINO-ACID OXIDASE | improved selectivity | Biological products | Immobilized enzymes | Lipase | Methods
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15. Full Text Enzymatic transesterification in a solvent-free system: synthesis of sn-2 docosahexaenoyl monoacylglycerol
by Moreno-Perez, Sonia and Luna, Pilar and Señorans, Javier and Rocha-Martin, Javier and Guisan, Jose M and Fernandez-Lorente, Gloria
Biocatalysis and Biotransformation, ISSN 1024-2422, 05/2018, Volume 36, Issue 3, pp. 265 - 270
The enzymatic transesterification of docosahexaenoic acid (DHA) ethyl ester with glycerol was carried out by using several immobilized lipases in a... 
interfacial adsorption of lipases | transesterification of DHA-EE with glycerol | novel DHA derivatives | Immobilized lipases | solvent-free systems | ETHYL-ESTERS | OMEGA-3-FATTY-ACIDS | TRIACYLGLYCEROLS | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | POLYUNSATURATED FATTY-ACIDS | FISH-OIL | LIPASE
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16. Full Text Cover Picture: Immobilizing Systems Biocatalysis for the Selective Oxidation of Glycerol Coupled to In Situ Cofactor Recycling and Hydrogen Peroxide Elimination (ChemCatChem 13/2015)
by Rocha-Martin, Javier and Acosta, Andreína and Guisan, Jose M and López-Gallego, Fernando
ChemCatChem, ISSN 1867-3880, 07/2015, Volume 7, Issue 13, pp. 1882 - 1882
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17. Full Text Two-Photon Fluorescence Anisotropy Imaging to Elucidate the Dynamics and the Stability of Immobilized Proteins
by Orrego, Alejandro H and García, Carolina and Mancheño, José M and Guisán, Jose M and Lillo, M. Pilar and López-Gallego, Fernando
The Journal of Physical Chemistry B, ISSN 1520-6106, 01/2016, Volume 120, Issue 3, pp. 485 - 491
Time/spatial-resolved fluorescence determines anisotropy values of supported-fluorescent proteins through different immobilization chemistries, evidencing some... 
CHEMISTRY, PHYSICAL | ENVIRONMENT | Photons | Immobilized Proteins - chemistry | Proteins - chemistry | Fluorescence Polarization - methods | Immobilized enzymes | Research | Anisotropy | Analysis
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18. Full Text Immobilizing Systems Biocatalysis for the Selective Oxidation of Glycerol Coupled to In Situ Cofactor Recycling and Hydrogen Peroxide Elimination
by Rocha‐Martin, Javier and Acosta, Andreína and Guisan, Jose M and López‐Gallego, Fernando
ChemCatChem, ISSN 1867-3880, 07/2015, Volume 7, Issue 13, pp. 1884 - 1884
The front cover artwork for Issue 13/2015 is provided by the groups of Prof. Jose M. Guisan, Instituto de Catálisis y Petroleoquímica (Spain) and Dr. Fernando... 
Glycerin | Hydrogen peroxide | Recycling (Waste, etc.) | Glycerol | Catalysis | Sugars | Monosaccharides
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19. Site-directing an intense multipoint covalent attachment (MCA) of mutants of the Geobacillus thermocatenulatus lipase 2 (BTL2): Genetic and chemical amination plus immobilization on a tailor-made support
by Godoy, Cesar A and de las Rivas, Blanca and Guisan, Jose M
Process Biochemistry, ISSN 1359-5113, 08/2014, Volume 49, Issue 8, pp. 1324 - 1331
Immobilized enzymes are preferred over their soluble counterparts due to their robustness in harsh industrial processes: the most stable enzyme derivatives are... 
Residues | Enzymes | Covalence | Immobilization | Genetics | Attachment | Derivatives | Lipase
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20. Immobilization of enzymes on heterofunctional epoxy supports
by Mateo, Cesar and Grazu, Valeria and Palomo, Jose M and Lopez-Gallego, Fernando and Fernandez-Lafuente, Roberto and Guisan, Jose M
Nature Protocols, ISSN 1754-2189, 05/2007, Volume 2, Issue 5, pp. 1022 - 1033
Immobilization of enzymes and proteins on activated supports permits the simplification of the reactor design and may be used to improve some enzyme... 
PENICILLIN-G ACYLASE | MULTIPOINT COVALENT ATTACHMENT | SALT-INDUCED IMMOBILIZATION | ACTIVATED SUPPORTS | BIOCHEMICAL RESEARCH METHODS | BETA-GALACTOSIDASE | THERMUS-SP STRAIN-T2 | LIPASE-LIPASE INTERACTIONS | ADDITIONAL STABILIZATION | AFFINITY SUPPORT | DIRECTED IMMOBILIZATION | Biotechnology - methods | Enzymes, Immobilized - chemistry | Proteins - chemistry | Bioreactors | Epoxy Compounds - chemistry
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