Human Gene Therapy, ISSN 1043-0342, 10/2011, Volume 22, Issue 10, pp. A1 - A138
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Human Gene Therapy, ISSN 1043-0342, 10/2012, Volume 23, Issue 10, pp. A1 - A173
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3.
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PEPito: A significantly improved non-viral episomal expression vector for mammalian cells
BMC Biotechnology, ISSN 1472-6750, 03/2010, Volume 10, Issue 1, pp. 20 - 20
Background: The episomal replication of the prototype vector pEPI-1 depends on a transcription unit starting from the constitutively expressed Cytomegalovirus...
SCAFFOLD/MATRIX-ATTACHED REGION | LONG-TERM | TRANSGENE EXPRESSION | NUCLEAR-MATRIX | GREEN-FLUORESCENT PROTEIN | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | IN-VIVO | GENE-EXPRESSION | PLASMID DNA VECTORS | MOUSE OOCYTE MEIOSIS | HISTONE ACETYLATION | Cytomegalovirus - genetics | NIH 3T3 Cells | Promoter Regions, Genetic | Gene Expression | Humans | Liver - metabolism | Male | Animals | Matrix Attachment Regions | Transfection | Plasmids - genetics | CpG Islands | Genetic Vectors - biosynthesis | Mice | Transgenes | Replicon | Episomes | Interferon beta | Physiological aspects | Genetic transcription | Gene therapy | Properties | Methods | Bioluminescence | Cloning | Genes | Colleges & universities | Experiments | Manuscripts | Mars | Plasmids | Cell cycle | DNA methylation | Standard deviation | Chromosomes | Binding sites
SCAFFOLD/MATRIX-ATTACHED REGION | LONG-TERM | TRANSGENE EXPRESSION | NUCLEAR-MATRIX | GREEN-FLUORESCENT PROTEIN | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | IN-VIVO | GENE-EXPRESSION | PLASMID DNA VECTORS | MOUSE OOCYTE MEIOSIS | HISTONE ACETYLATION | Cytomegalovirus - genetics | NIH 3T3 Cells | Promoter Regions, Genetic | Gene Expression | Humans | Liver - metabolism | Male | Animals | Matrix Attachment Regions | Transfection | Plasmids - genetics | CpG Islands | Genetic Vectors - biosynthesis | Mice | Transgenes | Replicon | Episomes | Interferon beta | Physiological aspects | Genetic transcription | Gene therapy | Properties | Methods | Bioluminescence | Cloning | Genes | Colleges & universities | Experiments | Manuscripts | Mars | Plasmids | Cell cycle | DNA methylation | Standard deviation | Chromosomes | Binding sites
Journal Article
Nature Medicine, ISSN 1078-8956, 08/2018, Volume 24, Issue 8, pp. 1192 - 1203
The oncometabolite (R)-2-hydroxyglutarate (R-2-HG) produced by isocitrate dehydrogenase (IDH) mutations promotes gliomagenesis via DNA and histone methylation....
GLIOMA-CELLS | MEDICINE, RESEARCH & EXPERIMENTAL | ACTIVATED PROTEIN-KINASE | ACID | DNA METHYLATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | ISOCITRATE DEHYDROGENASE MUTATIONS | CANCER | CELL BIOLOGY | IN-VIVO | DIFFERENTIATION | EXPRESSION | 2-HYDROXYGLUTARATE | Molecular genetics | Gliomas | Dosage and administration | Genetic aspects | Research | Genetic transcription | T cells | Drug therapy | Immunosuppressive agents | Transcription factors | Calcium | Transcription | Biosynthesis | Lymphocytes T | NF-AT protein | Immunity | Calcium signalling | Cancer vaccines | Lymphocytes | DNA methylation | Inhibition | Mutation | Methylation | Isocitrate dehydrogenase | Deoxyribonucleic acid--DNA | Tumors
GLIOMA-CELLS | MEDICINE, RESEARCH & EXPERIMENTAL | ACTIVATED PROTEIN-KINASE | ACID | DNA METHYLATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | ISOCITRATE DEHYDROGENASE MUTATIONS | CANCER | CELL BIOLOGY | IN-VIVO | DIFFERENTIATION | EXPRESSION | 2-HYDROXYGLUTARATE | Molecular genetics | Gliomas | Dosage and administration | Genetic aspects | Research | Genetic transcription | T cells | Drug therapy | Immunosuppressive agents | Transcription factors | Calcium | Transcription | Biosynthesis | Lymphocytes T | NF-AT protein | Immunity | Calcium signalling | Cancer vaccines | Lymphocytes | DNA methylation | Inhibition | Mutation | Methylation | Isocitrate dehydrogenase | Deoxyribonucleic acid--DNA | Tumors
Journal Article
Hepatology, ISSN 0270-9139, 09/2014, Volume 60, Issue 3, pp. 1035 - 1043
Host immune response to viral vectors, persistence of nonintegrating vectors, and sustained transgene expression are among the major challenges in gene...
SYSTEM | METHYLATION | THERAPY | HYPERPHENYLALANINEMIA | EPISOMAL TRANSGENE EXPRESSION | PLASMID DNA | MOUSE MODEL | IN-VIVO | GASTROENTEROLOGY & HEPATOLOGY | VECTORS | DELIVERY | Promoter Regions, Genetic | Liver - enzymology | Mice, Inbred C57BL | Male | Phenylketonurias - therapy | Animals | DNA, Superhelical | Female | Phenylalanine - blood | Genetic Vectors | Phenylalanine Hydroxylase - metabolism | Disease Models, Animal | Genetic Therapy - methods | Rodents | Liver | Hepatology | Deoxyribonucleic acid--DNA
SYSTEM | METHYLATION | THERAPY | HYPERPHENYLALANINEMIA | EPISOMAL TRANSGENE EXPRESSION | PLASMID DNA | MOUSE MODEL | IN-VIVO | GASTROENTEROLOGY & HEPATOLOGY | VECTORS | DELIVERY | Promoter Regions, Genetic | Liver - enzymology | Mice, Inbred C57BL | Male | Phenylketonurias - therapy | Animals | DNA, Superhelical | Female | Phenylalanine - blood | Genetic Vectors | Phenylalanine Hydroxylase - metabolism | Disease Models, Animal | Genetic Therapy - methods | Rodents | Liver | Hepatology | Deoxyribonucleic acid--DNA
Journal Article
PLoS ONE, ISSN 1932-6203, 10/2012, Volume 7, Issue 10, p. e47920
The development of genetically marked animal tumour xenografts is an area of ongoing research to enable easier and more reliable testing of cancer therapies....
GREEN FLUORESCENT PROTEIN | HEPATOCELLULAR-CARCINOMA | METHYLATION | ORIGIN | MAMMALIAN REPLICON | THERAPY | REPLICATION | MULTIDISCIPLINARY SCIENCES | PLASMID DNA | EXPRESSION | DELIVERY | Immunohistochemistry | Blotting, Southern | Humans | Ubiquitin C - genetics | Tumor Cells, Cultured - metabolism | DNA Primers - genetics | Mice, SCID | Matrix Attachment Regions - genetics | Plasmids - metabolism | Promoter Regions, Genetic - genetics | Genetic Vectors - genetics | Transplantation, Heterologous - methods | Animals | Luciferases | Plasmids - genetics | Polymerase Chain Reaction | Cell Line, Tumor | Mice | Longitudinal Studies | Real-Time Polymerase Chain Reaction | Liver cancer | Genetic vectors | Pancreatic cancer | Luciferase | Genetically modified organisms | Models | Genetic aspects | Gene expression | Health aspects | Ubiquitin | Heart | Biotechnology | Animal models | Liver | Genotoxicity | Hepatocellular carcinoma | Genomes | Hepatoma | Metastasis | Cancer therapies | Pancreatic carcinoma | Xenografts | DNA methylation | Plasmid DNA | Genetic modification | Deoxyribonucleic acid--DNA | Expression vectors | Cytomegalovirus | Tumor cell lines | Vectors (Biology) | Medicine | Artifacts | Plasmids | Cell lines | Epigenetics | In vivo methods and tests | Gene therapy | Tumors | Cancer | Deoxyribonucleic acid | DNA
GREEN FLUORESCENT PROTEIN | HEPATOCELLULAR-CARCINOMA | METHYLATION | ORIGIN | MAMMALIAN REPLICON | THERAPY | REPLICATION | MULTIDISCIPLINARY SCIENCES | PLASMID DNA | EXPRESSION | DELIVERY | Immunohistochemistry | Blotting, Southern | Humans | Ubiquitin C - genetics | Tumor Cells, Cultured - metabolism | DNA Primers - genetics | Mice, SCID | Matrix Attachment Regions - genetics | Plasmids - metabolism | Promoter Regions, Genetic - genetics | Genetic Vectors - genetics | Transplantation, Heterologous - methods | Animals | Luciferases | Plasmids - genetics | Polymerase Chain Reaction | Cell Line, Tumor | Mice | Longitudinal Studies | Real-Time Polymerase Chain Reaction | Liver cancer | Genetic vectors | Pancreatic cancer | Luciferase | Genetically modified organisms | Models | Genetic aspects | Gene expression | Health aspects | Ubiquitin | Heart | Biotechnology | Animal models | Liver | Genotoxicity | Hepatocellular carcinoma | Genomes | Hepatoma | Metastasis | Cancer therapies | Pancreatic carcinoma | Xenografts | DNA methylation | Plasmid DNA | Genetic modification | Deoxyribonucleic acid--DNA | Expression vectors | Cytomegalovirus | Tumor cell lines | Vectors (Biology) | Medicine | Artifacts | Plasmids | Cell lines | Epigenetics | In vivo methods and tests | Gene therapy | Tumors | Cancer | Deoxyribonucleic acid | DNA
Journal Article
Journal of Molecular Medicine, ISSN 0946-2716, 5/2011, Volume 89, Issue 5, pp. 515 - 529
We have previously described the development of a scaffold/matrix attachment region (S/MAR) episomal vector system for in vivo application and demonstrated its...
Human Genetics | Plasmid | Biomedicine | Minicircle | Scaffold/matrix attachment region (S/MAR) | Liver | Internal Medicine | Molecular Medicine | Gene therapy | Non-viral | Hydrodynamic delivery | TERM GENE-EXPRESSION | MEDICINE, RESEARCH & EXPERIMENTAL | CELLS | METHYLATION | MAR ELEMENTS | NUCLEAR | SCAFFOLD/MATRIX ATTACHMENT REGION | THERAPY | IN-VIVO | PLASMID DNA | GENETICS & HEREDITY | NONVIRAL VECTORS | Blotting, Southern | Animals | Enzyme-Linked Immunosorbent Assay | Humans | Liver - metabolism | Transgenes - genetics | Polymerase Chain Reaction | Cell Line, Tumor | Mice | Genetic Vectors - genetics | Anopheles | Genetic aspects | Research | Gene expression | Health aspects | Index Medicus
Human Genetics | Plasmid | Biomedicine | Minicircle | Scaffold/matrix attachment region (S/MAR) | Liver | Internal Medicine | Molecular Medicine | Gene therapy | Non-viral | Hydrodynamic delivery | TERM GENE-EXPRESSION | MEDICINE, RESEARCH & EXPERIMENTAL | CELLS | METHYLATION | MAR ELEMENTS | NUCLEAR | SCAFFOLD/MATRIX ATTACHMENT REGION | THERAPY | IN-VIVO | PLASMID DNA | GENETICS & HEREDITY | NONVIRAL VECTORS | Blotting, Southern | Animals | Enzyme-Linked Immunosorbent Assay | Humans | Liver - metabolism | Transgenes - genetics | Polymerase Chain Reaction | Cell Line, Tumor | Mice | Genetic Vectors - genetics | Anopheles | Genetic aspects | Research | Gene expression | Health aspects | Index Medicus
Journal Article
The Journal of Gene Medicine, ISSN 1099-498X, 06/2007, Volume 9, Issue 6, pp. 488 - 497
Background The delivery of a complete genomic DNA locus in vivo may prove advantageous for complementation gene therapy, especially when physiological...
genomic DNA | hydrodynamic | non‐viral gene therapy | bacterial artificial chromosome (BAC) | Bacterial artificial chromosome (BAC) | Non-viral gene therapy | Genomic DNA | Hydrodynamic | MEDICINE, RESEARCH & EXPERIMENTAL | FAMILIAL HYPERCHOLESTEROLEMIA | NONPHYSIOLOGICAL OVEREXPRESSION | non-viral gene therapy | HUMAN-CELLS | PHENOTYPIC CORRECTION | NAKED PLASMID DNA | INFECTIOUS DELIVERY | ADENOVIRUS-MEDIATED TRANSFER | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GENETICS & HEREDITY | DENSITY-LIPOPROTEIN-RECEPTOR | GENE-THERAPY | HERITABLE HYPERLIPIDEMIC RABBIT | Gene Transfer Techniques | Receptors, LDL - genetics | Humans | Liver - metabolism | DNA - metabolism | Plasmids - metabolism | Genome, Human - genetics | Chromosomes, Artificial, Bacterial - genetics | Injections | Animals | Time Factors | Base Pairing - genetics | beta-Galactosidase - metabolism | Female | Liver - cytology | Mice | Genetic Vectors | Transgenes
genomic DNA | hydrodynamic | non‐viral gene therapy | bacterial artificial chromosome (BAC) | Bacterial artificial chromosome (BAC) | Non-viral gene therapy | Genomic DNA | Hydrodynamic | MEDICINE, RESEARCH & EXPERIMENTAL | FAMILIAL HYPERCHOLESTEROLEMIA | NONPHYSIOLOGICAL OVEREXPRESSION | non-viral gene therapy | HUMAN-CELLS | PHENOTYPIC CORRECTION | NAKED PLASMID DNA | INFECTIOUS DELIVERY | ADENOVIRUS-MEDIATED TRANSFER | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GENETICS & HEREDITY | DENSITY-LIPOPROTEIN-RECEPTOR | GENE-THERAPY | HERITABLE HYPERLIPIDEMIC RABBIT | Gene Transfer Techniques | Receptors, LDL - genetics | Humans | Liver - metabolism | DNA - metabolism | Plasmids - metabolism | Genome, Human - genetics | Chromosomes, Artificial, Bacterial - genetics | Injections | Animals | Time Factors | Base Pairing - genetics | beta-Galactosidase - metabolism | Female | Liver - cytology | Mice | Genetic Vectors | Transgenes
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Systemic gene transfer of polyethylenimine (PEI)–plasmid DNA complexes to neonatal mice
Journal of Controlled Release, ISSN 0168-3659, 2011, Volume 150, Issue 3, pp. 298 - 306
Non-viral vectors have not been extensively investigated in neonatal mice due to the poor efficiency of the delivery methods available. Understanding the...
Scaffold matrix attachment region | Systemic injection | Mouse neonates | Gene transfer | PEI | Non-viral | CELLS | HEPATOCYTES | CHEMISTRY, MULTIDISCIPLINARY | DELIVERY | VECTOR | HEART | TRANSGENE EXPRESSION | IN-VIVO | PHARMACOLOGY & PHARMACY | FETAL | Gene Expression | Liver - enzymology | Liver - metabolism | DNA - administration & dosage | Brain - metabolism | DNA - chemistry | Kidney - metabolism | Animals | Transfection | Spleen - metabolism | Myocardium - metabolism | Polyethyleneimine - chemistry | Plasmids - chemistry | Female | Lung - metabolism | Mice | Transgenes | Plasmids - administration & dosage | Infants (Newborn) | DNA | Genetic research | Gene therapy | Gene expression | Health aspects | Genes
Scaffold matrix attachment region | Systemic injection | Mouse neonates | Gene transfer | PEI | Non-viral | CELLS | HEPATOCYTES | CHEMISTRY, MULTIDISCIPLINARY | DELIVERY | VECTOR | HEART | TRANSGENE EXPRESSION | IN-VIVO | PHARMACOLOGY & PHARMACY | FETAL | Gene Expression | Liver - enzymology | Liver - metabolism | DNA - administration & dosage | Brain - metabolism | DNA - chemistry | Kidney - metabolism | Animals | Transfection | Spleen - metabolism | Myocardium - metabolism | Polyethyleneimine - chemistry | Plasmids - chemistry | Female | Lung - metabolism | Mice | Transgenes | Plasmids - administration & dosage | Infants (Newborn) | DNA | Genetic research | Gene therapy | Gene expression | Health aspects | Genes
Journal Article
Molecular Therapy - Nucleic Acids, ISSN 2162-2531, 2013, Volume 2, Issue 8, pp. e115 - e115
The development of episomally maintained DNA vectors to genetically modify dividing cells efficiently and stably, without the risk of integration-mediated...
FOLLICULIN | MEDICINE, RESEARCH & EXPERIMENTAL | TGF-BETA | PROTEIN | PATHWAY | IN-VIVO | TRANSCRIPTION | HOGG-DUBE-SYNDROME | EXPRESSION | CANCER | TUMORIGENESIS | Original
FOLLICULIN | MEDICINE, RESEARCH & EXPERIMENTAL | TGF-BETA | PROTEIN | PATHWAY | IN-VIVO | TRANSCRIPTION | HOGG-DUBE-SYNDROME | EXPRESSION | CANCER | TUMORIGENESIS | Original
Journal Article
Human Gene Therapy, ISSN 1043-0342, 08/2011, Volume 22, Issue 8, pp. 915 - 923
Because of their high efficiency, virus-based vectors are currently used in most gene therapy trials. Because such vectors bear some potential safety risks,...
Reviews | MEDICINE, RESEARCH & EXPERIMENTAL | DNA-REPLICATION | MAMMALIAN REPLICON | TRANSGENE EXPRESSION | NUCLEAR-MATRIX | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | ORIGIN RECOGNITION COMPLEX | IN-VIVO | GENETICS & HEREDITY | S/MAR ELEMENT | GENE-TRANSFER | CIRCULAR YAC VECTORS | MITOTIC STABILITY | Gene Transfer Techniques | Genetic Therapy | Animals | Matrix Attachment Regions | Genetic Vectors - chemistry | Humans | Plasmids - genetics | Genetic Vectors - metabolism | DNA Replication | Mutagenesis, Insertional | Transgenes | Care and treatment | Genetic vectors | Genetic aspects | Research | Gene therapy | Health aspects | Cancer
Reviews | MEDICINE, RESEARCH & EXPERIMENTAL | DNA-REPLICATION | MAMMALIAN REPLICON | TRANSGENE EXPRESSION | NUCLEAR-MATRIX | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | ORIGIN RECOGNITION COMPLEX | IN-VIVO | GENETICS & HEREDITY | S/MAR ELEMENT | GENE-TRANSFER | CIRCULAR YAC VECTORS | MITOTIC STABILITY | Gene Transfer Techniques | Genetic Therapy | Animals | Matrix Attachment Regions | Genetic Vectors - chemistry | Humans | Plasmids - genetics | Genetic Vectors - metabolism | DNA Replication | Mutagenesis, Insertional | Transgenes | Care and treatment | Genetic vectors | Genetic aspects | Research | Gene therapy | Health aspects | Cancer
Journal Article
The Journal of Gene Medicine, ISSN 1099-498X, 06/2006, Volume 8, Issue 6, pp. 754 - 763
Background To develop more efficient non‐viral vectors, we have previously described a novel approach to attach a nuclear localisation signal (NLS) to plasmid...
DNA‐binding protein | Tat peptide | nuclear localisation signal | minicircle | gene transfer | DNA-binding protein | Minicircle | Gene transfer | Nuclear localisation signal | MEDICINE, RESEARCH & EXPERIMENTAL | TAT-FUSION PROTEINS | TRANSDUCTION DOMAIN | LOCALIZATION SIGNAL | TIME | MAMMALIAN-CELLS | CELLULAR UPTAKE | DELIVERY | PEPTIDES | TRANSGENE EXPRESSION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PLASMID DNA | GENETICS & HEREDITY | NIH 3T3 Cells | Gene Expression | Animals | Cell Nucleus - metabolism | Transfection - methods | beta-Galactosidase - metabolism | Tetracycline - metabolism | DNA, Circular - genetics | Female | Mice | Genetic Vectors | Transgenes
DNA‐binding protein | Tat peptide | nuclear localisation signal | minicircle | gene transfer | DNA-binding protein | Minicircle | Gene transfer | Nuclear localisation signal | MEDICINE, RESEARCH & EXPERIMENTAL | TAT-FUSION PROTEINS | TRANSDUCTION DOMAIN | LOCALIZATION SIGNAL | TIME | MAMMALIAN-CELLS | CELLULAR UPTAKE | DELIVERY | PEPTIDES | TRANSGENE EXPRESSION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PLASMID DNA | GENETICS & HEREDITY | NIH 3T3 Cells | Gene Expression | Animals | Cell Nucleus - metabolism | Transfection - methods | beta-Galactosidase - metabolism | Tetracycline - metabolism | DNA, Circular - genetics | Female | Mice | Genetic Vectors | Transgenes
Journal Article
ChemBioChem, ISSN 1439-4227, 04/2003, Volume 4, Issue 4, pp. 286 - 298
The impact of a peptide that contains a nuclear localisation sequence (NLS) on intracellular DNA trafficking was studied. We used the adenoviral core peptide...
gene therapy | peptides | lipids | gene technology | nuclear localisation sequence | Lipids | Nuclear localisation sequence | Peptides | Gene technology | Gene therapy | CHEMISTRY, MEDICINAL | MEDIATE EFFICIENT TRANSFECTION | CATIONIC LIPOSOMES | BIOCHEMISTRY & MOLECULAR BIOLOGY | ADENOVIRUS CORE | PEPTIDE-MU MU | PSEUDO-PROLINES | POLY(ETHYLENE GLYCOL) | PLASMID DNA | IN-VIVO | TRACHEAL EPITHELIAL-CELLS | BACTERIAL-DNA | Glycoside Hydrolases - genetics | Humans | Peptides - genetics | Polyethylene Glycols - chemistry | Recombinant Fusion Proteins - metabolism | Carbocyanines | Cholesterol - chemistry | Peptides - metabolism | Transfection | Adenoviridae - genetics | Electrophoretic Mobility Shift Assay | Oligopeptides - chemistry | Cell Line | Cholesterol - analogs & derivatives | Gene Transfer Techniques | Peptides - chemistry | Oligopeptides - genetics | DNA - metabolism | Oligopeptides - metabolism | Recombinant Fusion Proteins - chemistry | Microscopy, Confocal | DNA - chemistry | Nuclear Localization Signals - chemistry | Plasmids | Intracellular Space - metabolism | Liposomes | Genetic Vectors | Phosphatidylethanolamines - chemistry | Fluorescent Dyes
gene therapy | peptides | lipids | gene technology | nuclear localisation sequence | Lipids | Nuclear localisation sequence | Peptides | Gene technology | Gene therapy | CHEMISTRY, MEDICINAL | MEDIATE EFFICIENT TRANSFECTION | CATIONIC LIPOSOMES | BIOCHEMISTRY & MOLECULAR BIOLOGY | ADENOVIRUS CORE | PEPTIDE-MU MU | PSEUDO-PROLINES | POLY(ETHYLENE GLYCOL) | PLASMID DNA | IN-VIVO | TRACHEAL EPITHELIAL-CELLS | BACTERIAL-DNA | Glycoside Hydrolases - genetics | Humans | Peptides - genetics | Polyethylene Glycols - chemistry | Recombinant Fusion Proteins - metabolism | Carbocyanines | Cholesterol - chemistry | Peptides - metabolism | Transfection | Adenoviridae - genetics | Electrophoretic Mobility Shift Assay | Oligopeptides - chemistry | Cell Line | Cholesterol - analogs & derivatives | Gene Transfer Techniques | Peptides - chemistry | Oligopeptides - genetics | DNA - metabolism | Oligopeptides - metabolism | Recombinant Fusion Proteins - chemistry | Microscopy, Confocal | DNA - chemistry | Nuclear Localization Signals - chemistry | Plasmids | Intracellular Space - metabolism | Liposomes | Genetic Vectors | Phosphatidylethanolamines - chemistry | Fluorescent Dyes
Journal Article