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investigating or analysing materials by determining theirchemical or physical properties (26) 26
measuring (26) 26
physics (26) 26
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processes of preparing such compositions (3) 3
proteins (3) 3
rats (3) 3
streptavidin (3) 3
xanthenes (3) 3
analysis (2) 2
antibodies - chemistry (2) 2
assay (2) 2
avidin (2) 2
avidin - chemistry (2) 2
biotin - chemistry (2) 2
cell biology (2) 2
chromatography, affinity - methods (2) 2
combinatorial chemistry (2) 2
compositions thereof (2) 2
culture media (2) 2
fermentation or enzyme-using processes to synthesise a desiredchemical compound or composition or to separate optical isomersfrom a racemic mixture (2) 2
fluorescein-5-isothiocyanate (2) 2
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organic dyes or closely-related compounds for producing dyes (2) 2
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proteins - chemistry (2) 2
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1. Full Text Coupling of antibodies with biotin
by Haugland, Rosaria P and You, Wendy W
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2008, Volume 418, p. 13
The avidin-biotin bond is the strongest known biological interaction between a ligand and a protein (Kd = 1.3 x 10-15 M at pH 5.0) (1). The affinity is so high... 
Biotin - chemistry | Antibodies - chemistry | Binding Sites, Antibody
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2. Full Text Preparation of avidin conjugates
by Haugland, Rosaria P and Bhalgat, Mahesh K
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2008, Volume 418, p. 1
The high-affinity avidin-biotin system has found applications in different fields of biotechnology, including immunoassays, histochemistry, affinity... 
Enzymes - chemistry | Antibodies - chemistry | Avidin - chemistry | Chromatography, Affinity - methods | Succinimides - chemistry
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3. Full Text Conjugation of antibodies to biotin
by Hirsch, James D and Haugland, Rosaria P
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2005, Volume 295, pp. 135 - 154
Antibodies can be conjugated to biotin by a number of chemical means. They can then be used in immunochemical procedures in conjunction with secondary reagents... 
Biotin - chemistry | Avidin - chemistry | Animals | Immunochemistry | Immunoglobulin Fab Fragments - chemistry
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4. Full Text Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates
by Berlier, Judith E and Rothe, Anca and Buller, Gayle and Bradford, Jolene and Gray, Diane R and Filanoski, Brian J and Telford, William G and Yue, Stephen and Liu, Jixiang and Cheung, Ching-Ying and Chang, Wesley and Hirsch, James D and Beechem, Joseph M and Haugland, Rosaria P and Haugland, Richard P
Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, 12/2003, Volume 51, Issue 12, pp. 1699 - 1712
Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm,... 
Flow cytometry | Cy dyes | Microscopy | Fluorescent Bioconjugates | Photostability | Alexa Fluor dyes | FRET | Immunofluorescence | Long-wavelength dyes | fluorescent bioconjugates | FLUOROCHROMES | LABELING REAGENTS | photostability | PROBES | CELL BIOLOGY | ANTIGEN-DETECTION | flow cytometry | microscopy | long-wavelength dyes | FLOW-CYTOMETRY | SUCCINIMIDYL ESTERS | PROTEINS | AVIDIN | BINDING | immunofluorescence | BRIGHT | Immunohistochemistry | Water | Succinates - chemistry | Carbocyanines - chemistry | Humans | Benzopyrans - chemistry | Flow Cytometry | Cattle | Brain - ultrastructure | Epithelial Cells - cytology | Photobleaching | Fluorescent Dyes - chemistry | Cells, Cultured | Solubility | Epithelial Cells - ultrastructure | Rats | Mitochondrial Proton-Translocating ATPases - metabolism | Pulmonary Artery - enzymology | Pulmonary Artery - cytology | Pulmonary Artery - ultrastructure | Animals | T-Lymphocytes - cytology | Proteins - chemistry | Spectrophotometry | Indoles - chemistry | Microscopy, Fluorescence
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5. Full Text Alexa Dyes, a Series of New Fluorescent Dyes that Yield Exceptionally Bright, Photostable Conjugates
by Panchuk–Voloshina, Nataliya and Haugland, Rosaria P and Bishop–Stewart, Janell and Bhalgat, Mahesh K and Millard, Paul J and Mao, Fei and Leung, Wai-Yee and Haugland, Richard P
Journal of Histochemistry and Cytochemistry, ISSN 0022-1554, 09/1999, Volume 47, Issue 9, pp. 1179 - 1188
Alexa 350, Alexa 430, Alexa 488, Alexa 532, Alexa 546, Alexa 568, and Alexa 594 dyes are a new series of fluorescent dyes with emission/excitation spectra... 
Flow cytometry | Dyes | Fluorescent conjugates | Microscopy | Immunofluorescence | Photostability | ANTIBODIES | IMMUNOFLUORESCENCE MICROSCOPY | dyes | photostability | FLUOROPHORE | CELL BIOLOGY | LISSAMINE RHODAMINE | flow cytometry | microscopy | TEXAS RED | fluorescent conjugates | DERIVATIVES | immunofluorescence | Xanthenes | Propidium | Animals | Fluorescein-5-isothiocyanate | Microscopy, Fluorescence - methods | Photochemistry - methods | Humans | Cells, Cultured | Streptavidin | Fluorescent Dyes
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6. Full Text Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation
by Hirsch, James D and Eslamizar, Leila and Filanoski, Brian J and Malekzadeh, Nabi and Haugland, Richard P and Haugland, Rosaria P and Beechem, Joseph M
Analytical Biochemistry, ISSN 0003-2697, 2002, Volume 308, Issue 2, pp. 343 - 357
The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the... 
Succinimidyl ester | Bioconjugates | Amine-reactive | Affinity chromatography | Desthiobiotin | Streptavidin | Reversible binding | Biotin | Avidin | BIOTINYLATION | affinity chromatography | CHEMISTRY, ANALYTICAL | streptavidin | avidin | RECOGNITION | reversible binding | ANALOGS | desthiobiotin | BIOCHEMISTRY & MOLECULAR BIOLOGY | biotin | BIOCHEMICAL RESEARCH METHODS | SELECTIVE RETRIEVAL | SUBUNITS | MULTILAYERS | amine-reactive | bioconjugates | LIGANDS | succinimidyl ester | AFFINITY COLUMNS | TECHNOLOGY | RESIDUES | Recombinant Proteins - metabolism | Rabbits | Humans | Avidin - metabolism | Tubulin - metabolism | Animals | Carrier Proteins - metabolism | Streptavidin - metabolism | Immunoglobulin G - immunology | Sepharose - chemistry | Protein Binding | Biotin - metabolism | Indicators and Reagents | Ligands | Chromatography, Affinity - methods | Biotin - analogs & derivatives | Immunoglobulin G - metabolism
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7. Full Text Vitronectin interaction with glycosaminoglycans. Kinetics, structural determinants, and role in binding to endothelial cells
by François, Patrice P and Preissner, Klaus T and Herrmann, Mathias and Haugland, Rosaria P and Vaudaux, P and Lew, Daniel P and Krause, Karl-Heinz
Journal of Biological Chemistry, ISSN 0021-9258, 12/1999, Volume 274, Issue 53, pp. 37611 - 37619
Vitronectin (VN) is a high affinity heparin-binding protein. The physiological role of this binding has hitherto received little attention, and its molecular... 
SELF-ASSOCIATION | ADHESION | COAGULASE-NEGATIVE STAPHYLOCOCCI | BIOCHEMISTRY & MOLECULAR BIOLOGY | PLASMINOGEN-ACTIVATOR INHIBITOR-1 | ANTITHROMBIN | AUREUS | HEPARAN-SULFATE PROTEOGLYCANS | MULTIMERIC VITRONECTIN | IDENTIFICATION | S-PROTEIN VITRONECTIN | Endothelium, Vascular - cytology | Heparin - metabolism | Endothelium, Vascular - metabolism | Humans | Cells, Cultured | Vitronectin - chemistry | Protein Binding | Spectrometry, Fluorescence | Protein Conformation | Kinetics | Vitronectin - metabolism
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8. Full Text Green/red dual fluorescence detection of total protein and alkaline phosphate‐conjugated probes on blotting membranes
by Top, Karen Pretty On and Hatleberg, Gayle and Berggren, Kiera N and Ryan, Diane and Kemper, Courtenay and Haugland, Rosaria P and Patton, Wayne F
ELECTROPHORESIS, ISSN 0173-0835, 04/2001, Volume 22, Issue 5, pp. 896 - 905
A two‐color fluorescence detection method is described based upon covalently coupling the succinimidyl ester of BODIPY FL‐X to proteins immobilized on... 
Fluorescence | Electroblot | Protein stain | Dichromatic | Immunodetection | BLOTS | CHEMISTRY, ANALYTICAL | protein stain | BIOCHEMICAL RESEARCH METHODS | electroblot | 2-DIMENSIONAL GEL-ELECTROPHORESIS | WHEAT-GERM-AGGLUTININ | CONCANAVALIN-A | COLLOIDAL GOLD | immunodetection | EGG-WHITE | fluorescence | dichromatic | PROTEOMICS | OVALBUMIN | Acridines - chemistry | Boron Compounds - chemistry | Phosphates | Blotting, Western - methods | Egg Proteins - analysis | Rats | Spectrometry, Fluorescence | Proteins - analysis | Alkaline Phosphatase | Tubulin - analysis | Animals | Carboxylic Acids | Cattle | Polyvinyls | Indicators and Reagents | Brain Chemistry | Electrophoresis - methods | Glycoproteins - analysis | Fluorescent Dyes | Fibroblasts - chemistry
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9. Full Text Preparation of avidin conjugates
by Haugland, Rosaria P and Bhalgat, Mahesh K
Methods in Molecular Biology, ISSN 1064-3745, 12/2007, Volume 418, pp. 1 - 12
Conference Proceeding
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10. Full Text Coupling of antibodies with biotin
by Haugland, Rosaria P and You, Wendy W
Methods in Molecular Biology, ISSN 1064-3745, 12/2007, Volume 418, pp. 13 - 23
Conference Proceeding
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11. Full Text 5-(Pentafluorobenzoylamino)fluorescein: A Selective Substrate for the Determination of Glutathione Concentration and Glutathione S-Transferase Activity
by Arttamangkul S and Bhalgat M.K and Haugland R.P and Diwu Z and Liu J and Klaubert D.H
Analytical Biochemistry, ISSN 0003-2697, 05/1999, Volume 269, Issue 2, pp. 410 - 417
5-(Pentafluorobenzoylamino)fluorescein (PFB-F), a new thiol-reactive molecule was synthesized to improve the detection limits and specificity of the assays for... 
CELLS | HOMOCYSTEINE | CHEMISTRY, ANALYTICAL | ASSAY | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | PERFORMANCE LIQUID-CHROMATOGRAPHY | THIOLS | ELECTROCHEMICAL DETECTION | MONOCHLOROBIMANE | RESISTANCE | NITROGEN MUSTARDS | EXPRESSION | Fluorescent Dyes - chemical synthesis | Chromatography, High Pressure Liquid - methods | Chromatography, Thin Layer - methods | Humans | Recombinant Fusion Proteins - analysis | Substrate Specificity | Fluoresceins - chemical synthesis | Endothelium, Vascular - enzymology | Endothelium, Vascular - chemistry | Glutathione Transferase - analysis | Animals | Cattle | Glutathione - analysis
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12. Full Text Quenched BODIPY Dye-Labeled Casein Substrates for the Assay of Protease Activity by Direct Fluorescence Measurement
by Jones, Laurie J and Upson, Rosalyn H and Haugland, Richard P and Haugland, Rosaria P and Panchuk-Voloshina, Nataliya and Zhou, Mingjie
Analytical Biochemistry, ISSN 0003-2697, 09/1997, Volume 251, Issue 2, pp. 144 - 152
We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an... 
K CO BIOCHEMICAL RESEARCH METHODS | K EA CHEMISTRY, ANALYTICAL | CHEMISTRY, ANALYTICAL | K CQ BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | ENERGY-TRANSFER | PROTEINS | Hydrolysis | Xanthenes | Animals | Protease Inhibitors - analysis | Caseins - chemistry | Fluorescein-5-isothiocyanate | Fluorescent Antibody Technique, Direct | Humans | Spectrometry, Fluorescence | Endopeptidases - analysis | Boron Compounds | Fluorescent Dyes | Measurement | Biological products | Casein | Protease inhibitors | Proteases | Proteolysis | Analysis | Fluorescein | Fluorescence
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13. Full Text p75 and TrkA receptors are both required for uptake of NGF in adult sympathetic neurons: use of a novel fluorescent NGF conjugate
by Gatzinsky, Kliment P and Haugland, Rosaria P and Thrasivoulou, Christopher and Orike, Nina and Budi-Santoso, Agus W and Cowen, Timothy
Brain Research, ISSN 0006-8993, 2001, Volume 920, Issue 1, pp. 226 - 238
We have developed and tested the biological activity and specificity of a novel fluorescent dextran-Texas Red-nerve growth factor (DTR-NGF) conjugate. DTR-NGF... 
Confocal microscopy | Rat | NGF transport | Antibody receptor blockade | Superior cervical ganglion | RETROGRADE AXONAL-TRANSPORT | rat | TYROSINE KINASE | AFFINITY NEUROTROPHIN RECEPTOR | superior cervical ganglion | CROSS-LINKING | COMPARTMENTED CULTURES | NEUROSCIENCES | antibody receptor blockade | NERVE GROWTH-FACTOR | SIGNAL-TRANSDUCTION | FACTOR BINDING | confocal microscopy | BIOLOGICAL-ACTIVITY | PC12 CELLS | Iris - metabolism | Sympathetic Nervous System - cytology | Antibodies, Blocking | Rats | Axonal Transport - physiology | Male | Nerve Growth Factors - metabolism | Rats, Sprague-Dawley | Sympathetic Nervous System - metabolism | Dose-Response Relationship, Drug | Xanthenes | Microscopy, Confocal | Animals | Dextrans | Image Processing, Computer-Assisted | Receptor, trkA - metabolism | Iris - innervation | Neurons - metabolism | Receptor, Nerve Growth Factor - metabolism | Fluorescent Dyes
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14. Full Text 3-(4-Carboxybenzoyl)quinoline-2-carboxaldehyde, a Reagent with Broad Dynamic Range for the Assay of Proteins and Lipoproteins in Solution
by You, Wendy W and Haugland, Richard P and Haugland, Rosaria P and Ryan, Diane K
Analytical Biochemistry, ISSN 0003-2697, 01/1997, Volume 244, Issue 2, pp. 277 - 282
This paper describes the application of 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA), a sensitive fluorogenic reagent for detection of amines, to the... 
K CO BIOCHEMICAL RESEARCH METHODS | K EA CHEMISTRY, ANALYTICAL | CHEMISTRY, ANALYTICAL | MICROGRAM QUANTITIES | K CQ BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | PRIMARY AMINES | FLUORESCAMINE | Quinolines | Benzoates | Molecular Weight | Solutions | Spectrometry, Fluorescence | Sulfhydryl Compounds - chemistry | Lipoproteins - chemistry | Proteins - chemistry | Fluorescent Dyes | Dithiothreitol - pharmacology | Proteins | Quinoline | Alkaloids | Biological products | Blood lipids | Chemical tests and reagents
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15. Full Text A Spectrophotometric Method to Measure Enzymatic Activity in Reactions That Generate Inorganic Pyrophosphate
by Upson, Rosalyn H and Haugland, Richard P and Haugland, Rosaria P and Malekzadeh, Mohammad N
Analytical Biochemistry, ISSN 0003-2697, 12/1996, Volume 243, Issue 1, pp. 41 - 45
This paper describes a spectrophotometric assay that can measure the inorganic pyrophosphate produced from various enzymatic reactions. This is a coupled assay... 
BIOCHEMISTRY & MOLECULAR BIOLOGY | ASSAY | Guanosine - analogs & derivatives | Spectrophotometry, Atomic | Luciferases - metabolism | Acetate-CoA Ligase - metabolism | Thionucleosides | Luminescent Measurements | Diphosphates - analysis | Measurement | Phosphates | Biological products | Ligases | Methods
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16. Full Text Texas Red-X and Rhodamine Red-X, New Derivatives of Sulforhodamine 101 and Lissamine Rhodamine B with Improved Labeling and Fluorescence Properties
by Lefevre, Charles and Kang, Hee Chol and Haugland, Rosaria P and Malekzadeh, Nabi and Arttamangkul, Seksiri and Haugland, Richard P
Bioconjugate Chemistry, ISSN 1043-1802, 07/1996, Volume 7, Issue 4, pp. 482 - 489
Texas Red sulfonyl chloride (TR-SC) and Lissamine rhodamine B sulfonyl chloride (LRB-SC) are popular dyes often used to prepare red fluorescent conjugates that... 
CHEMISTRY | FLUOROPHORES | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS
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17. Full Text Green- and red-fluorescent nanospheres for the detection of cell surface receptors by flow cytometry
by Bhalgat, Mahesh K and Haugland, Rosaria P and Pollack, Jeffrey S and Swan, Sharon and Haugland, Richard P
Journal of Immunological Methods, ISSN 0022-1759, 10/1998, Volume 219, Issue 1-2, pp. 57 - 68
Fluorescent probes serve as sensitive tools for obtaining structural and functional information in cellular systems. In spite of the high sensitivity provided... 
Microspheres | CD4 | Phycobiliproteins | CD3 | Beads | Epidermal growth factor receptor | Latex | microspheres | epidermal growth factor receptor | BIOCHEMICAL RESEARCH METHODS | beads | IMMUNOLOGY | EPIDERMAL GROWTH-FACTOR | latex | phycobiliproteins | Flow Cytometry - methods | Jurkat Cells | Humans | Receptors, Cell Surface - analysis | Fluorescent Dyes - metabolism | Spectrometry, Fluorescence | CD3 Complex - analysis | Animals | CD4 Antigens - analysis | Polystyrenes | Fluorescein | Phycoerythrin | Streptavidin | Mice | Tumor Cells, Cultured | 3T3 Cells | ErbB Receptors - analysis
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18. Full Text Fluorescent rhodol derivatives: Versatile, photostable labels and tracers
by Whitaker, J.E and Whitaker, James E and Haugland, Rosaria P and Haugland, R.P and Ryan, D and Ryan, Diane and Hewitt, Peter C and Hewitt, P.C and Haugland, R.P and Haugland, Richard P and Prendergast, Franklyn G and Prendergast, F.G
Analytical Biochemistry, ISSN 0003-2697, 1992, Volume 207, Issue 2, pp. 267 - 279
A series of chemically reactive, fluorescent rhodol derivatives was prepared and evaluated. Reactive functional groups included activated esters, amines,... 
LASER-DYES | RED | CHARGE SEPARATION | BLUE | BIOCHEMISTRY & MOLECULAR BIOLOGY | Fluorescent Dyes - chemical synthesis | Xanthenes - chemical synthesis | Fluorescent Dyes - chemistry | Affinity Labels - chemistry | Xanthenes - chemistry | Drug Stability | Spectrometry, Fluorescence | Structure-Activity Relationship | Flow Cytometry | Indicators and Reagents | Molecular Structure | Spectrophotometry | Microscopy, Fluorescence | Affinity Labels - chemical synthesis | Hydrogen-Ion Concentration
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19. Full Text Vitronectin Interaction with Glycosaminoglycans
by Patrice P. François and Klaus T. Preissner and Mathias Herrmann and Rosaria P. Haugland and P. Vaudaux and Daniel P. Lew and Karl-Heinz Krause
Journal of Biological Chemistry, ISSN 0021-9258, 12/1999, Volume 274, Issue 53, p. 37611
Vitronectin (VN) is a high affinity heparin-binding protein. The physiological role of this binding has hitherto received little attention, and its molecular... 
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20. Full Text 5-(Pentafluorobenzoylamino)fluorescein: A Selective Substrate for the Determination of Glutathione Concentration and GlutathioneS-Transferase Activity
by Arttamangkul, Seksiri and Bhalgat, Mahesh K and Haugland, Richard P and Haugland, Rosaria P and Diwu, Zhenjun and Liu, Jixiang and Klaubert, Dieter H
Analytical Biochemistry, ISSN 0003-2697, 05/1999, Volume 269, Issue 2, pp. 410 - 417
Cysteine | Biological products | Thiols | Isoenzymes | Analysis | Fluorescein | Goods and services tax
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