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Nature Neuroscience, ISSN 1097-6256, 07/2011, Volume 14, Issue 7, pp. 874 - 880
UNC119 is widely expressed among vertebrates and other phyla. We found that UNC119 recognized the acylated N terminus of the rod photoreceptor transducin alpha... 
PHOTORECEPTOR PROTEIN | RETINAL DEGENERATION | TRANSDUCIN | COMPLEX | LIGHT-DRIVEN TRANSLOCATION | CAENORHABDITIS-ELEGANS | PDE-DELTA | UNC-119 | C-ELEGANS | BINDING PROTEIN | NEUROSCIENCES | Adaptor Proteins, Signal Transducing - chemistry | Protein Binding - genetics | Models, Chemical | Humans | Dark Adaptation - genetics | Green Fluorescent Proteins - genetics | GTP-Binding Protein alpha Subunits, G12-G13 - metabolism | Transducin - deficiency | Time Factors | Cattle | Transducin - metabolism | Sensory Receptor Cells - metabolism | Glycine - genetics | Transducin - genetics | GTP-Binding Protein alpha Subunits - metabolism | Animals, Genetically Modified | Gene Expression Regulation - genetics | GTP-Binding Protein alpha Subunits, G12-G13 - genetics | Gene Expression Regulation - physiology | Models, Molecular | Signal Transduction - genetics | Mutation - genetics | Protein Transport - genetics | Mice, Knockout | Caenorhabditis elegans | Animals | GTP Phosphohydrolases - metabolism | Adaptor Proteins, Signal Transducing - genetics | GTP-Binding Protein alpha Subunits, Gi-Go | Protein Structure, Quaternary - genetics | GTP-Binding Protein alpha Subunits - deficiency | Mice | Adaptor Proteins, Signal Transducing - metabolism | Caenorhabditis elegans Proteins - genetics | Physiological aspects | Genetic aspects | G proteins | Research | Neurons | Genes | Index Medicus
Journal Article
Nucleic Acids Research, ISSN 0305-1048, 07/2012, Volume 40, Issue 1, pp. W542 - W546
We describe the RPF web server, a quality assessment tool for protein NMR structures. The RPF server measures the 'goodness-of-fit' of the 3D structure with... 
STRUCTURE VALIDATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | Algorithms | Protein Multimerization | Nuclear Magnetic Resonance, Biomolecular | Protein Conformation | Software | Internet | Proteins - chemistry | Quality Control | Index Medicus
Journal Article
Molecular & Cellular Proteomics, ISSN 1535-9476, 10/2008, Volume 7, Issue 10, pp. 2048 - 2060
Journal Article
Journal Article
Journal of Structural Biology, ISSN 1047-8477, 10/2010, Volume 172, Issue 1, pp. 21 - 33
We describe the core Protein Production Platform of the Northeast Structural Genomics Consortium (NESG) and outline the strategies used for producing... 
Parallel protein purification | High-throughput protein production | Laboratory Information Management System | Protein Structure Initiative | T7 Escherichia coli expression system | 6X-His tag | Structural genomics | Ligation-independent cloning | Wheat germ cell-free | NMR microprobe screening | X-ray crystallography | NMR | Construct optimization | HDX-MS | Disorder prediction | Multiple Displacement Amplification | HIGH-LEVEL | MACROMOLECULES | ESCHERICHIA-COLI | CELL BIOLOGY | CRYSTALLIZATION | DYNAMIC LIGHT-SCATTERING | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOPHYSICS | 17 Escherichia coli expression system | EXPRESSION | Recombinant Proteins - metabolism | Reproducibility of Results | Magnetic Resonance Spectroscopy | Electrophoresis, Polyacrylamide Gel | Recombinant Proteins - chemistry | Proteins - genetics | Recombinant Proteins - isolation & purification | Proteins - metabolism | Escherichia coli - genetics | Databases, Protein | Cloning, Molecular | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | Proteins - chemistry | Proteomics - methods | Genomics - methods | Consortia | Proteins | Genetic research | Genomics | Escherichia coli | Index Medicus | Core protein | Wheat Germ Cell Free | Structural Genomics | High throughput protein production | Ligation independent cloning | T7 Escherichia Coli expression system
Journal Article
2011, Methods in Enzymology, ISBN 9780123812742, Volume 493, 40
In this chapter, we concentrate on the production of high-quality protein samples for nuclear magnetic resonance (NMR) studies. In particular, we provide an... 
Parallel protein purification | High-throughput protein production | Condensed single protein production | T7 Escherichia coli expression system | Laboratory information management system | Structural genomics | Ligation-independent cloning | Total gene synthesis | Protein structure initiative | NMR microprobe screening | Wheat germ cell free | NMR | Multiple displacement amplification | Construct optimization | HDX-MS | Disorder prediction | 6×-His tag | HIGH-LEVEL | DYNAMIC LIGHT-SCATTERING | RECOMBINANT PROTEIN | GENOMICS CONSORTIUM | BIOCHEMISTRY & MOLECULAR BIOLOGY | ESCHERICHIA-COLI | BIOCHEMICAL RESEARCH METHODS | LIGATION-INDEPENDENT CLONING | GENE-EXPRESSION | MULTIPLE DISPLACEMENT AMPLIFICATION | CODON USAGE | PRODUCTION PLATFORM | Plant Proteins - isolation & purification | Computational Biology | Fermentation | Proteins - isolation & purification | Nuclear Magnetic Resonance, Biomolecular - methods | Escherichia coli Proteins - biosynthesis | Cloning, Molecular | Escherichia coli - metabolism | Isotope Labeling | Proteins - chemistry | Proteomics - methods | Triticum - chemistry | Genomics - methods | Small Molecule Libraries - isolation & purification | Index Medicus | Laboratory Information Management System | Protein Structure Initiative | 6X-His tag | Wheat Germ Cell Free | Structural Genomics | High throughput protein production | Ligation independent cloning | condensed single protein production | Multiple Displacement Amplification
Book Chapter
Methods in Enzymology, ISSN 0076-6879, 01/2019, Volume 615, pp. 453 - 475
Cell surface molecules are important for development and function of multicellular organisms. Although several methods are available to identify... 
X-ray crystallography | Glycosylation | Protein–protein interaction screen | NMR spectroscopy | Gene synthesis | HEK cells | Mammalian cell surface proteins | Small-angle X-ray scattering
Conference Proceeding
Methods in Molecular Biology, ISSN 1064-3745, 2014, Volume 1091, pp. 3 - 16
Conference Proceeding
Methods in Enzymology, ISSN 0076-6879, 01/2019, Volume 614, pp. 363 - 392
Accurate protein structure determination by solution-state NMR is challenging for proteins greater than about 20 kDa, for which extensive perdeuteration is... 
AutoStructure/ASDP | Hybrid methods | Protein NMR spectroscopy | Multiple sequence alignment | Automated NMR data analysis | Evolutionary couplings | Maximum entropy | Protein families
Conference Proceeding
Structure, ISSN 0969-2126, 02/2009, Volume 17, Issue 2, pp. 151 - 159
Journal Article