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Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 12/2007, Volume 104, Issue 50, pp. 19948 - 19953
To characterize proteins associated with active transcription complexes, we purified RNA polymerase II (pol II) from Saccharomyces cerevisiae after fixing live... 
Proteins | Yeasts | Imidazoles | RNA | Gels | DNA | Genes | Histones | Peptide elongation factors | Resins | Transcription | RNA processing | Affinity purification | Mass spectrometry | Nascent RNP | transcription | MEDIATOR | MULTIDISCIPLINARY SCIENCES | affinity purification | IDENTIFICATION | MASS-SPECTROMETRY | YEAST | TANDEM AFFINITY PURIFICATION | MESSENGER-RNA | TREX COMPLEX | mass spectrometry | nascent RNP | BINDING | ELONGATION-FACTORS | Fungal Proteins - chemistry | Cross-Linking Reagents | Transcriptional Activation - genetics | Oligonucleotide Array Sequence Analysis - methods | RNA-Binding Proteins - chemistry | Ribonucleoproteins | Fungal Proteins - genetics | Saccharomyces cerevisiae Proteins - genetics | RNA-Binding Proteins - isolation & purification | Tandem Mass Spectrometry | RNA Polymerase II - isolation & purification | RNA Processing, Post-Transcriptional - genetics | Protein Subunits - isolation & purification | RNA Polymerase II - genetics | Saccharomyces cerevisiae Proteins - isolation & purification | Protein Subunits - chemistry | Fungal Proteins - isolation & purification | RNA Polymerase II - chemistry | Protein Subunits - genetics | Saccharomyces cerevisiae Proteins - chemistry | Polymerase chain reaction | Crosslinking | Genetic aspects | Research | Saccharomyces | Biological Sciences
Journal Article