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biotechnology & applied microbiology (5) 5
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Journal Article
Journal of Biotechnology, ISSN 0168-1656, 06/2018, Volume 276-277, pp. 34 - 41
[Display omitted] •Intensified process developed for cSAV production.•Volumetric yield improved by 36-fold through high-cell-density cultivation.•Refolding... 
Temperature-based refolding | Process intensification | Core streptavidin | High-cell-density cultivation | Escherichia coli | INCLUSION-BODY PROTEINS | SOLUBILIZATION | MONOMERIC STREPTAVIDIN | ESCHERICHIA-COLI | STRATEGIES | FED-BATCH FERMENTATIONS | SPECTROSCOPY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PURIFICATION | BIOTIN | EXPRESSION | Specific gravity | Analysis | Methods
Journal Article
Biochemical Engineering Journal, ISSN 1369-703X, 08/2012, Volume 67, pp. 88 - 96
► VLP yield and quality by dialysis and dilution assembly compared quantitatively. ► Process intensification leading to unit operation elimination. ► New... 
Self-assembly | Bioprocess design | Virus-like particles | Downstream processing | Process integration | Scale-up | POLYOMA-VIRUS | STRUCTURAL PROTEIN | CAPSID PROTEIN | DISULFIDE BONDS | CALCIUM-IONS | ESCHERICHIA-COLI | VACCINE | ENGINEERING, CHEMICAL | FIELD-FLOW FRACTIONATION | HIGH-LEVEL EXPRESSION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | MURINE POLYOMAVIRUS
Journal Article
Biochemical Engineering Journal, ISSN 1369-703X, 10/2012, Volume 68, pp. 120 - 128
► Rate of assembly buffer exchange dramatically affects VLP yield and quality. ► VLP assembly diafiltration reactor designed with optimized buffer exchange... 
Self-assembly | Bioprocess design | Virus-like particles | Downstream processing | Process integration | Scale-up | STRUCTURAL PROTEIN | ESCHERICHIA-COLI | VACCINE | SACCHAROMYCES-CEREVISIAE | ENGINEERING, CHEMICAL | FIELD-FLOW FRACTIONATION | POLYOMAVIRUS | ULTRAFILTRATION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PURIFICATION | AGGREGATION
Journal Article
Process Biochemistry, ISSN 1359-5113, 03/2020, Volume 90, pp. 204 - 214
[Display omitted] •Extraction and two-stage purification strategy was developed to obtain rHN (∼95%).•Quantitative ELISA developed using purified rHN reference... 
Hemagglutinin-neuraminidase | Purification | Newcastle disease virus | Vaccine quantitation | Subunit vaccine | ELISA
Journal Article
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