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pichia - genetics (19) 19
pichia pastoris (15) 15
molecular sequence data (14) 14
saccharomyces-cerevisiae (13) 13
yeast (13) 13
biochemistry & molecular biology (12) 12
pichia - metabolism (12) 12
amino acid sequence (11) 11
index medicus (11) 11
biotechnology & applied microbiology (10) 10
cloning, molecular (10) 10
microbiology (9) 9
fungal proteins - genetics (8) 8
proteins (8) 8
expression (7) 7
fungal proteins - metabolism (7) 7
gene (7) 7
gene expression (7) 7
genetic vectors (7) 7
glycosylation (7) 7
mutation (7) 7
plasmids (7) 7
base sequence (6) 6
gene expression regulation, fungal (6) 6
genetics & heredity (6) 6
methylotrophic yeast (6) 6
saccharomyces cerevisiae - genetics (6) 6
sequence homology, amino acid (6) 6
alcohol oxidoreductases - genetics (5) 5
biochemical research methods (5) 5
genes (5) 5
hansenula-polymorpha (5) 5
humans (5) 5
membrane proteins - metabolism (5) 5
recombinant fusion proteins - genetics (5) 5
recombinant proteins (5) 5
recombinant proteins - biosynthesis (5) 5
recombinant proteins - genetics (5) 5
research (5) 5
secretion (5) 5
sequence analysis, dna (5) 5
escherichia coli (4) 4
fermentation (4) 4
gene deletion (4) 4
genes, fungal (4) 4
membrane proteins - genetics (4) 4
methanol - metabolism (4) 4
mutagenesis (4) 4
mutagenesis, site-directed (4) 4
mutants (4) 4
pichia - growth & development (4) 4
proteases (4) 4
purification (4) 4
recombinant expression (4) 4
vesicular transport proteins (4) 4
yeasts (4) 4
analysis (3) 3
animals (3) 3
cell biology (3) 3
cloning (3) 3
conserved sequence (3) 3
dna (3) 3
escherichia coli - genetics (3) 3
foreign gene expression (3) 3
fungal proteins - chemistry (3) 3
genes, reporter (3) 3
genetic aspects (3) 3
genetic complementation test (3) 3
golgi apparatus - chemistry (3) 3
heterologous protein production (3) 3
localization (3) 3
methanol utilization pathway (3) 3
peptides (3) 3
peroxisome biogenesis (3) 3
phenotype (3) 3
promoter regions, genetic (3) 3
protein binding (3) 3
protein import (3) 3
protein structure, tertiary (3) 3
proteolysis (3) 3
recombinant fusion proteins - metabolism (3) 3
saccharomyces cerevisiae - enzymology (3) 3
saccharomyces cerevisiae proteins (3) 3
targeting signal (3) 3
transformation (3) 3
vacuoles - chemistry (3) 3
3-ketoacyl-coa thiolase (2) 2
acid alpha-mannosidase (2) 2
alcohol oxidase (2) 2
alcohol oxidase 1 gene promoter (2) 2
alcohol oxidase genes (2) 2
alleles (2) 2
amyloid precursor protein (2) 2
anti-hiv agents (2) 2
antiviral agents (2) 2
assembly mutants (2) 2
bacterial proteins - biosynthesis (2) 2
biogenesis (2) 2
biological transport - physiology (2) 2
bioreactors (2) 2
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Journal Article
Journal Article
Molecular and Cellular Biology, ISSN 0270-7306, 02/2006, Volume 26, Issue 3, pp. 883 - 897
Journal Article
The Journal of Cell Biology, ISSN 0021-9525, 4/1997, Volume 137, Issue 1, pp. 79 - 92
Mutations in the S. cerevisiae VPS29 and VPS30 genes lead to a selective protein sorting defect in which the vacuolar protein carboxypeptidase Y (CPY) is... 
Yeasts | Receptors | Phenotypes | Plasmids | Alleles | Cell membranes | Vacuoles | Cells | Endosomes | P branes | CYTOPLASMIC DOMAIN | TRANSFORMATION | FACTOR-II RECEPTOR | LOCALIZATION | CARBOXYPEPTIDASE-Y | MUTANTS | TRANSPORT | K DR CELL BIOLOGY | COMPARTMENT | YEAST SECRETORY PATHWAY | SACCHAROMYCES-CEREVISIAE | CELL BIOLOGY | Cell organelles | Research | Mutation (Biology) | Golgi apparatus
Journal Article
Protein Expression and Purification, ISSN 1046-5928, 2009, Volume 67, Issue 2, pp. 175 - 181
Journal Article
Protein Expression and Purification, ISSN 1046-5928, 07/2010, Volume 72, Issue 1, pp. 113 - 124
The Escherichia coli maltose binding protein (MBP) has been utilized as a translational fusion partner to improve the expression of foreign proteins made in E.... 
Recombinant expression | Pichia pastoris | Fusion protein | Proteolysis | Secretion | Maltose binding protein | DOMAIN | BIOCHEMISTRY & MOLECULAR BIOLOGY | STRUCTURAL-ANALYSIS | AFFINITY TAGS | BIOCHEMICAL RESEARCH METHODS | FUSION PARTNERS | SACCHAROMYCES-CEREVISIAE | EXPRESSION SYSTEM | SOLUBILITY | YEAST | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | RECOMBINANT PROTEINS | PURIFICATION | Periplasmic Binding Proteins - chemistry | Amino Acid Sequence | Recombinant Fusion Proteins - isolation & purification | Gene Expression | Mutagenesis, Site-Directed | Molecular Sequence Data | Escherichia coli Proteins - metabolism | Recombinant Fusion Proteins - chemistry | Escherichia coli Proteins - isolation & purification | Recombinant Fusion Proteins - metabolism | Mutagenesis | Escherichia coli - genetics | Pichia - genetics | Escherichia coli Proteins - genetics | Recombinant Fusion Proteins - genetics | Escherichia coli Proteins - chemistry | Maltose-Binding Proteins | Periplasmic Binding Proteins - genetics | Periplasmic Binding Proteins - isolation & purification | Periplasmic Binding Proteins - metabolism | Proteins | Proteases | Escherichia coli | Physiological aspects | Chemical properties | Mass spectrometry | Sugars | Protein binding | Index Medicus | recombinant expression | fusion protein | secretion | maltose binding protein | proteolysis
Journal Article