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hiv infections - virology (8) 8
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1. HIV-1 DNA Decay Dynamics in Blood During More Than a Decade of Suppressive Antiretroviral Therapy
by Guillaume J Besson and Christina M Lalama and Ronald J Bosch and Rajesh T Gandhi and Margaret A Bedison and Evgenia Aga and Sharon A Riddler and Deborah K McMahon and Feiyu Hong and John W Mellors
Clinical Infectious Diseases, ISSN 1058-4838, 11/2014, Volume 59, Issue 9, p. 1312
  Human immunodeficiency virus type 1 (HIV-1) DNA dynamics during long-term antiretroviral therapy (ART) are not defined. Blood mononuclear cells obtained... 
Polymerase chain reaction | Antiretroviral drugs | Median | Deoxyribonucleic acid--DNA | Blood
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2. Full Text HIV-1 DNA decay dynamics in blood during more than a decade of suppressive antiretroviral therapy
by Besson, Guillaume J and Lalama, Christina M and Bosch, Ronald J and Gandhi, Rajesh T and Bedison, Margaret A and Aga, Evgenia and Riddler, Sharon A and McMahon, Deborah K and Hong, Feiyu and Mellors, John W
Clinical Infectious Diseases, ISSN 1058-4838, 11/2014, Volume 59, Issue 9, pp. 1312 - 1321
Background. Human immunodeficiency virus type 1 (HIV-1) DNA dynamics during long-term antiretroviral therapy (ART) are not defined. Methods. Bloodmononuclear... 
HIV-1 persistence | HIV-1 DNA decay | antiretroviral therapy | immune activation | INFECTIOUS DISEASES | CD4(+) T-CELLS | MICROBIOLOGY | HIV-1-INFECTED PATIENTS | IMMUNOLOGY | VIRAL REPLICATION | RALTEGRAVIR INTENSIFICATION | VIREMIA | IN-VIVO | IMMUNODEFICIENCY-VIRUS TYPE-1 | PCR ASSAY | HAART | Anti-Retroviral Agents - therapeutic use | HIV-1 - drug effects | HIV Infections - virology | Humans | Middle Aged | Anti-Retroviral Agents - pharmacology | Linear Models | Male | CD4 Lymphocyte Count | HIV-1 - genetics | Viral Load | DNA, Viral - blood | Young Adult | HIV Infections - immunology | Viremia - virology | Adult | Female | HIV Infections - drug therapy | Longitudinal Studies | Antiviral agents | Immunospecificity | DNA damage | Analysis | Dosage and administration | HIV infection | Risk factors | AIDS | HIV | antiretroviral therapy; HIV-1 DNA decay
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3. Full Text Improved single-copy assays for quantification of persistent HIV-1 viremia in patients on suppressive antiretroviral therapy
by Cillo, Anthony R and Vagratian, David and Bedison, Margaret A and Anderson, Elizabeth M and Kearney, Mary F and Fyne, Elizabeth and Koontz, Dianna and Coffin, John M and Piatak, Michael and Mellors, John W
Journal of Clinical Microbiology, ISSN 0095-1137, 11/2014, Volume 52, Issue 11, pp. 3944 - 3951
A quantitative real-time PCR (qRT-PCR) assay with single-copy sensitivity targeting HIV-1 gag RNA (the gag single-copy assay [gSCA]) has been used widely to... 
LOW-LEVEL VIREMIA | PLASMA VIREMIA | REVERSE-TRANSCRIPTASE | IMMUNODEFICIENCY-VIRUS TYPE-1 | DYNAMICS | MARKERS | MICROBIOLOGY | INFECTION | TIME | INTENSIFICATION | LYMPHOCYTES | Anti-Retroviral Agents - therapeutic use | HIV Infections - virology | Humans | Real-Time Polymerase Chain Reaction - methods | RNA, Viral - analysis | RNA, Viral - blood | DNA Primers - genetics | RNA, Viral - genetics | Viremia - virology | HIV-1 - isolation & purification | Oligonucleotide Probes - genetics | Sensitivity and Specificity | HIV Infections - drug therapy | Viral Load - methods | Virology
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4. Full Text Early antiretroviral therapy in South African children reduces HIV-1-infected cells and cell-associated HIV-1 RNA in blood mononuclear cells
by Van Zyl, Gert U and Bedison, Margaret A and Van Rensburg, Anita Janse and Laughton, Barbara and Cotton, Mark F and Mellors, John W
Journal of Infectious Diseases, ISSN 0022-1899, 07/2015, Volume 212, Issue 1, pp. 39 - 43
We measured cell-associated human immunodeficiency virus (HIV)-1 DNA (CAD) and RNA (CAR) and plasma HIV-1 RNA in blood samples from 20 children in the Children... 
HIV-1 cell associated RNA | transcriptionally active proviruses | HIV-1 single copy assay | measures of HIV-1 persistence | Early infant antiretroviral therapy | HIV-1 cell-associated DNA | INFECTIOUS DISEASES | RHESUS-MONKEYS | QUANTIFICATION | PERSISTENCE | CD4(+) T-CELLS | MICROBIOLOGY | IMMUNOLOGY | VIREMIA | early infant antiretroviral therapy | Anti-Retroviral Agents - therapeutic use | HIV Infections - virology | Humans | RNA, Viral - analysis | DNA, Viral - analysis | Secondary Prevention | HIV-1 - genetics | Leukocytes, Mononuclear - virology | Viral Load | HIV-1 - isolation & purification | Blood - virology | HIV Infections - drug therapy | Child | Major and Brief Reports
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5. Residual plasma viraemia and infectious HIV-1 recovery from resting memory CD4 cells in patients on antiretroviral therapy: Results from ACTG A5173
by Gandhi, Rajesh T and Bosch, Ronald J and Aga, Evgenia and Bedison, Margaret A and Bastow, Barbara and Schmitz, John L and Siliciano, Janet D and Siliciano, Robert F and Eron, Joseph J and Mellors, John W and ACTG A5173 Team
Antiviral Therapy, ISSN 1359-6535, 2013, Volume 18, Issue 4, pp. 607 - 613
Background: In HIV-1-infected patients receiving antiretroviral therapy (ART), the relationship between residual viraemia and ex vivo recovery of infectious... 
INFECTIOUS DISEASES | PHARMACOLOGY & PHARMACY | SUBPOPULATIONS | VIROLOGY
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6. Full Text New Tools for Quantifying HIV-1 Reservoirs: Plasma RNA Single Copy Assays and Beyond
by Hilldorfer, Benedict B and Cillo, Anthony R and Besson, Guillaume J and Bedison, Margaret Anne and Mellors, John W
Current HIV/AIDS Reports, ISSN 1548-3568, 3/2012, Volume 9, Issue 1, pp. 91 - 100
Quantification of plasma HIV-1 RNA below the limit of FDA-approved assays by a single copy quantitative PCR assays (SCA) has provided significant insights into... 
Ultrasensitive PCR | HIV reservoirs | Medicine & Public Health | HIV treatment intensification | Single copy assay | HIV quantification | Residual viremia | Medicine/Public Health, general | INFECTIOUS DISEASES | Reproducibility of Results | Viremia - virology | HIV-1 - isolation & purification | Disease Reservoirs - virology | HIV Infections - virology | Humans | Sensitivity and Specificity | RNA, Viral - blood | HIV-1 - genetics | Polymerase Chain Reaction - methods | Virus diseases | HIV testing | RNA | HIV (Viruses)
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7. Residual plasma viraemia and infectious HIV-1 recovery from resting memory CD4 cells in patients on antiretroviral therapy: results from ACTG A5173
by Gandhi, Rajesh T and Bosch, Ronald J and Aga, Evgenia and Bedison, Margaret A and Bastow, Barbara and Schmitz, John L and Siliciano, Janet D and Siliciano, Robert F and Eron, Joseph J and Mellors, John W and ACTG A5173 team
Antiviral therapy, 2013, Volume 18, Issue 4, p. 607
In HIV-1-infected patients receiving antiretroviral therapy (ART), the relationship between residual viraemia and ex vivo recovery of infectious virus from... 
HIV Infections - blood | Gene Expression | ADP-ribosyl Cyclase 1 - genetics | HIV-1 - drug effects | HIV Infections - virology | Humans | RNA, Viral - blood | Viremia - drug therapy | Immunologic Memory - drug effects | CD4-Positive T-Lymphocytes - immunology | HIV Infections - immunology | HIV-1 - immunology | Viremia - virology | HIV Envelope Protein gp41 - therapeutic use | ADP-ribosyl Cyclase 1 - immunology | Anti-HIV Agents - therapeutic use | HIV Infections - drug therapy | Viremia - immunology | Peptide Fragments - therapeutic use | CD4-Positive T-Lymphocytes - virology | Viral Load - drug effects | Viremia - blood | CD4-Positive T-Lymphocytes - drug effects
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8. Full Text Continued slow decay of the residual plasma viremia level in HIV-1-infected adults receiving long-term antiretroviral therapy
by Riddler, Sharon A and Aga, Evgenia and Bosch, Ronald J and Bastow, Barbara and Bedison, Margaret and Vagratian, David and Vaida, Florin and Eron, Joseph J and Gandhi, Rajesh T and Mellors, John W and ACTG A5276S Protocol Team and ACTG A5276s Protocol Team
Journal of Infectious Diseases, ISSN 0022-1899, 02/2016, Volume 213, Issue 4, pp. 556 - 560
We measured plasma human immunodeficiency virus type 1 (HIV-1) RNA levels by means of single-copy assay in 334 participants receiving virologically suppressive... 
HIV/AIDS | singlecopy assay | viral decay | residual viremia | CD4/CD8 ratio | HIV-1 | INFECTIOUS DISEASES | single-copy assay | PERSISTENCE | CD4(+) T-CELLS | MICROBIOLOGY | INFECTION | IMMUNOLOGY | HIV Infections - virology | Humans | Male | RNA, Viral - blood | Plasma - virology | Viral Load | CD4-CD8 Ratio | HIV-1 - isolation & purification | Anti-Retroviral Agents - administration & dosage | Adult | Female | HIV Infections - drug therapy | Antiretroviral Therapy, Highly Active - methods | Longitudinal Studies | Antiviral agents | Care and treatment | Analysis | Dosage and administration | HIV infection | Risk factors | Blood plasma | Major and Brief Reports | CD4 | HIV | CD8 ratio | AIDS
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9. Full Text Early combination antiretroviral therapy limits exposure to HIV-1 replication and cell-associated HIV-1 DNA levels in infants
by McManus, Margaret and Mick, Eric and Hudson, Richard and Mofenson, Lynne M and Sullivan, John L and Somasundaran, Mohan and Luzuriaga, Katherine and PACTG 356 Investigators
PLoS ONE, ISSN 1932-6203, 04/2016, Volume 11, Issue 4, p. e0154391
The primary aim of this study was to measure HIV-1 persistence following combination antiretroviral therapy (cART) in infants and children. Peripheral blood... 
DNA, Viral - biosynthesis | Virus Replication - genetics | Humans | Child, Preschool | Infant | Male | Leukocytes, Mononuclear - virology | DNA Copy Number Variations | RNA, Viral - antagonists & inhibitors | HIV-1 - physiology | RNA, Viral - genetics | HIV Infections - diagnosis | DNA, Viral - antagonists & inhibitors | Female | Virus Replication - drug effects | Anti-Retroviral Agents - therapeutic use | Leukocytes, Mononuclear - drug effects | Time-to-Treatment | HIV-1 - drug effects | HIV Infections - virology | RNA, Viral - biosynthesis | Treatment Outcome | Antiretroviral Therapy, Highly Active | HIV Infections - drug therapy | DNA, Viral - genetics | Viral Load - drug effects | Longitudinal Studies | Highly active antiretroviral therapy | Usage | Control | HIV (Viruses) | Plasma | Therapy | Pediatrics | Perinatal exposure | Replication initiation | Infections | Infants | Medical schools | Acquired immune deficiency syndrome--AIDS | Antiretroviral agents | Lymphocytes | Human immunodeficiency virus--HIV | Peripheral blood mononuclear cells | Children | Drug therapy | Age | Deoxyribonucleic acid--DNA | Antiretroviral drugs | Statistical analysis | Exposure | Ribonucleic acid--RNA | Generalized linear models | Antiretroviral therapy | DNA biosynthesis | Medicine | Replication | RNA | Acquired immune deficiency syndrome | Deoxyribonucleic acid | Ribonucleic acid | AIDS | HIV | DNA | Human immunodeficiency virus
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10. Full Text Paucity of intact non-induced provirus with early, long-Term antiretroviral therapy of perinatal HIV infection
by Rainwater-Lovett, Kaitlin and Ziemniak, Carrie and Watson, Douglas and Luzuriaga, Katherine and Siberry, George and Petru, Ann and Chen, Ya Hui and Uprety, Priyanka and Mcmanus, Margaret and Ho, Ya-Chi and Lamers, Susanna L and Persaud, Deborah
PLoS ONE, ISSN 1932-6203, 02/2017, Volume 12, Issue 2, p. e0170548
The latent reservoir is a major barrier to HIV eradication. Reservoir size is emerging as an important biomarker to assess the likelihood of HIV remission in... 
REPLICATION | VIRUS TYPE-1 RECOMBINATION | VIROLOGICAL CONTROL | IMMUNE-RESPONSES | MULTIDISCIPLINARY SCIENCES | CD4(+) T-CELLS | HIV-1-INFECTED CHILDREN | PERIPHERAL-BLOOD | CURE | LATENT RESERVOIR | SUPPRESSION | Virus Latency | HIV Infections - virology | Humans | Child, Preschool | Infant | CD4 Lymphocyte Count | Pregnancy Complications, Infectious | Viral Load | Antiretroviral Therapy, Highly Active | CD4-Positive T-Lymphocytes - immunology | Pregnancy | HIV Infections - immunology | HIV-1 - physiology | HIV Antibodies - immunology | Proviruses - genetics | Adolescent | Female | HIV Infections - drug therapy | Mutation | CD4-Positive T-Lymphocytes - virology | Child | DNA, Viral | Antiviral agents | Dosage and administration | HIV infection in children | Genetic aspects | Perinatal infection | Drug therapy | Adolescence | Therapy | Pediatrics | Infections | Genomes | Lymphocytes T | Acquired immune deficiency syndrome--AIDS | Lymphocytes | Antiretroviral agents | Human immunodeficiency virus--HIV | Remission | Children | Adolescents | Age | Deoxyribonucleic acid--DNA | Antiretroviral drugs | Antiretroviral therapy | CD4 antigen | Medicine | Infectious diseases | Biomarkers | Replication | Adults | Acquired immune deficiency syndrome | Deoxyribonucleic acid | AIDS | HIV | DNA | Human immunodeficiency virus
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