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1. Full Text Real-time, portable genome sequencing for Ebola surveillance
by Quick, Joshua and Loman, Nicholas J and Duraffour, Sophie and Simpson, Jared T and Severi, Ettore and Cowley, Lauren and Bore, Joseph Akoi and Koundouno, Raymond and Dudas, Gytis and Mikhail, Amy and Ouédraogo, Nobila and Afrough, Babak and Bah, Amadou and Baum, Jonathan H. J and Becker-Ziaja, Beate and Boettcher, Jan Peter and Cabeza-Cabrerizo, Mar and Camino-Sánchez, Álvaro and Carter, Lisa L and Doerrbecker, Juliane and Enkirch, Theresa and Garciá-Dorival, Isabel and Hetzelt, Nicole and Hinzmann, Julia and Holm, Tobias and Kafetzopoulou, Liana Eleni and Koropogui, Michel and Kosgey, Abigael and Kuisma, Eeva and Logue, Christopher H and Mazzarelli, Antonio and Meisel, Sarah and Mertens, Marc and Michel, Janine and Ngabo, Didier and Nitzsche, Katja and Pallasch, Elisa and Patrono, Livia Victoria and Portmann, Jasmine and Repits, Johanna Gabriella and Rickett, Natasha Y and Sachse, Andreas and Singethan, Katrin and Vitoriano, Inês and Yemanaberhan, Rahel L and Zekeng, Elsa G and Racine, Trina and Bello, Alexander and Sall, Amadou Alpha and Faye, Ousmane and Faye, Oumar and Magassouba, N'Faly and Williams, Cecelia V and Amburgey, Victoria and Winona, Linda and Davis, Emily and Gerlach, Jon and Washington, Frank and Monteil, Vanessa and Jourdain, Marine and Bererd, Marion and Camara, Alimou and Somlare, Hermann and Camara, Abdoulaye and Gerard, Marianne and Bado, Guillaume and Baillet, Bernard and Delaune, Déborah and Nebie, Koumpingnin Yacouba and Diarra, Abdoulaye and Savane, Yacouba and Pallawo, Raymond Bernard and Gutierrez, Giovanna Jaramillo and Milhano, Natacha and Roger, Isabelle and Williams, Christopher J and Yattara, Facinet and Lewandowski, Kuiama and Taylor, James and Rachwal, Phillip and Turner, Daniel J and Pollakis, Georgios and Hiscox, Julian A and Matthews, David A and O'Shea, Matthew K and Johnston, Andrew McD and Wilson, Duncan and Hutley, Emma and Smit, Erasmus and Di Caro, Antonino and Wolfel, Roman and Stoecker, Kilian and Fleischmann, Erna and Gabriel, Martin and Weller, Simon A and Koivogui, Lamine and Diallo, Boubacar and Keita, Sakoba and Rambaut, Andrew and Formenty, Pierre and ...
Nature, ISSN 0028-0836, 02/2016, Volume 530, Issue 7589, pp. 228 - 232
The Ebola virus disease epidemic in West Africa is the largest on record, responsible for over 28,599 cases and more than 11,299 deaths(1). Genome sequencing... 
TRANSMISSION | VIRUS | EVOLUTION | DNA | MULTIDISCIPLINARY SCIENCES | SIERRA-LEONE | DYNAMICS | OUTBREAK | NANOPORE SEQUENCER | Ebolavirus - classification | Genome, Viral - genetics | Humans | Mutagenesis - genetics | Ebolavirus - pathogenicity | Mutation Rate | Disease Outbreaks - statistics & numerical data | Ebolavirus - genetics | Guinea - epidemiology | Hemorrhagic Fever, Ebola - epidemiology | Sequence Analysis, DNA - instrumentation | Epidemiological Monitoring | Time Factors | Hemorrhagic Fever, Ebola - virology | Aircraft | Sequence Analysis, DNA - methods | Ebola virus | Distribution | Genetic aspects | Nucleotide sequencing | Methods | DNA sequencing | Prevalence studies (Epidemiology) | Electrical currents | Pathogens | Surveillance | Laboratories | Phylogenetics | Universal Serial Bus | Genomes | Bioinformatics | Deoxyribonucleic acid--DNA
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2. Full Text Rickettsia massiliae and rickettsia conorii israeli spotted fever strain differentially regulate endothelial cell responses
by Bechelli, Jeremy and Smalley, Claire and Milhano, Natacha and Walker, David H and Fang, Rong
PLoS ONE, ISSN 1932-6203, 09/2015, Volume 10, Issue 9, p. e0138830
Rickettsiae primarily target microvascular endothelial cells. However, it remains elusive how endothelial cell responses to rickettsiae play a role in the... 
APOPTOSIS | ACTIVATION | MOLECULAR-DETECTION | INFLAMMATION | MULTIDISCIPLINARY SCIENCES | INFECTION | TICK-BITE FEVER | NF-KAPPA-B | VASCULAR ENDOTHELIUM | TIGHT JUNCTION | EXPRESSION | Capillary Permeability | Species Specificity | Humans | Cell Membrane Permeability | Cercopithecus aethiops | Caspase 1 - metabolism | Rickettsia Infections - genetics | Boutonneuse Fever - metabolism | Endothelial Cells - microbiology | Rickettsia Infections - metabolism | Cytokines - genetics | Real-Time Polymerase Chain Reaction | Vero Cells | Rickettsia conorii - genetics | Cell Line | Rickettsia - genetics | Rickettsia - physiology | Cytokines - metabolism | Enzyme-Linked Immunosorbent Assay | Cell Survival | Endothelial Cells - metabolism | Rickettsia Infections - microbiology | Gene Expression Regulation | Boutonneuse Fever - genetics | Reverse Transcriptase Polymerase Chain Reaction | Host-Pathogen Interactions | Boutonneuse Fever - microbiology | Animals | DNA, Bacterial - genetics | Rickettsia conorii - physiology | Infection | Medical research | Cell death | Mortality | Medicine, Experimental | Protein biosynthesis | Permeability | Health aspects | Endothelium | Cell culture | Dehydrogenases | Transcription | Pathogenesis | Virulence | Staining | Homology | Infections | Dehydrogenase | Caspase-1 | Interleukin 6 | Next-generation sequencing | Significance | Interleukin 8 | Statistical analysis | Cytokines | Rickettsiosis | Caspase | Lactate dehydrogenase | Inflammation | Pharmacology | Gene expression | Fever | L-Lactate dehydrogenase | Endothelial cells | Pathology | Protein synthesis | Monolayers | Fatalities | Microvasculature | Annexin V | Lactic acid | Chemokines | Monocyte chemoattractant protein 1 | Apoptosis
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3. Full Text Circulating enterovirus genotypes in Norway, 2014–2015: A reason for concern?
by Milhano, Natacha and Vainio, Kirsti and Christensen, Andreas and Dudman, Susanne
Journal of Clinical Virology, ISSN 1386-6532, 2016, Volume 82, pp. S122 - S123
Infectious Disease | Allergy and Immunology
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4. Full Text Circulation and diagnostics of Puumala virus in Norway: nephropatia epidemica incidence and rodent population dynamics
by Milhano, Natacha and Korslund, Lars and Evander, Magnus and Ahlm, Clas and Vainio, Kirsti and Dudman, Susanne G and Andreassen, Åshild and Medicinska fakulteten and Infektionssjukdomar and Institutionen för klinisk mikrobiologi and Virologi and Umeå universitet
APMIS, ISSN 0903-4641, 08/2017, Volume 125, Issue 8, pp. 732 - 742
Hantaviruses pose a public health concern worldwide causing haemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Puumala... 
Hantavirus | Puumala virus | bank vole | nephropathia epidemica | PCR | VOLES | HEMORRHAGIC-FEVER | HUMAN HANTAVIRUS INFECTIONS | MICROBIOLOGY | IMMUNOLOGY | PATHOLOGY | Serum - virology | Humans | Middle Aged | Child, Preschool | Infant | Male | Hemorrhagic Fever with Renal Syndrome - diagnosis | Arvicolinae - growth & development | Phylogeny | Incidence | Sequence Homology | Young Adult | Hemorrhagic Fever with Renal Syndrome - epidemiology | Puumala virus - isolation & purification | Polymerase Chain Reaction | Puumala virus - genetics | Aged, 80 and over | Adult | Female | Seasons | Child | Real-Time Polymerase Chain Reaction | Infant, Newborn | Sequence Analysis, DNA | Hantavirus Pulmonary Syndrome - epidemiology | Topography, Medical | Hantavirus Pulmonary Syndrome - diagnosis | Animals | Norway - epidemiology | Adolescent | Hantavirus Pulmonary Syndrome - virology | Aged | Puumala virus - classification | Hemorrhagic Fever with Renal Syndrome - virology | Cluster Analysis | Population Dynamics | Analysis | Population biology | Epidemics | Kidneys | Viruses | Hantavirus pulmonary syndrome | Clustering | Fever | Inhalation | Polymerase chain reaction | Dynamic tests | Diagnostic software | Hemorrhagic fever with renal syndrome | Population dynamics | Diagnostic systems | Respiration | Public health | Kidney transplantation | Clinical Medicine | Medical and Health Sciences | Medicin och hälsovetenskap | Infektionsmedicin | Infectious Medicine | Klinisk medicin
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5. Full Text Management strategies of enterovirus D68 outbreaks: Current perspectives
by Milhano, Natacha and Borge, Kaja Sverdrup and Bragstad, Karoline and Dudman, Susanne G
Virus Adaptation and Treatment, ISSN 1179-1624, 03/2018, Volume 10, pp. 1 - 7
Following its discovery in California in 1962, enterovirus D68 (EV-D68) was reported only sporadically around the world. In August 2014, a marked increase of... 
Enterovirus D68 | Outbreak | Treatment | AFP | Diagnostics | Surveillance | Infection | Strategic planning (Business) | Paralysis | Epidemiology | Lung diseases | Illnesses | Disease | Respiratory diseases | Viruses | Nervous system | Infections | Genomes | Public health | treatment | outbreak | diagnostics | surveillance
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6. Full Text In vitroandin vivocomparison of transport media for detecting nasopharyngeal carriage ofStreptococcus pneumoniae
by Steens, Anneke and Milhano, Natacha and Aaberge, Ingeborg S and Vestrheim, Didrik F
PeerJ, ISSN 2167-8359, 09/2016, Volume 4, p. e2449
BackgroundAs a standard method for pneumococcal carriage studies, the World Health Organization recommends nasopharyngeal swabs be transported and stored at... 
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7. Full Text In vitro and in vivo comparison of transport media for detecting nasopharyngeal carriage of Streptococcus pneumoniae
by Steens, Anneke and Milhano, Natacha and Aaberge, Ingeborg S and Vestrheim, Didrik F
PeerJ, ISSN 2167-8359, 2016, Volume 2016, Issue 9, p. e2449
Background. As a standard method for pneumococcal carriage studies, the World Health Organization recommends nasopharyngeal swabs be transported and stored at... 
In vivo and in vitro comparison | Nasopharyngeal carriage | Carriage study | Pneumococcus | Transport media | Streptococcus pneumoniae | PNEUMOCOCCAL CONJUGATE VACCINE | RESPIRATORY VIRUSES | SEROTYPES | MULTIDISCIPLINARY SCIENCES | COLONIZATION | CHILDREN | Glycerin | Glycerol | Pneumonia | Bacterial pneumonia | Analysis | Temperature | Media (enrichment) | Microbiology | Working groups | Colonies | Antimicrobial agents | Latex agglutination | Vaccines | Glucose | Latex | Media (transport) | Streptococcus infections | Studies | Agglutination | Children | Public health | Deoxyribonucleic acid--DNA | Milk
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8. Full Text In vitro and in vivo comparison of transport media for detecting nasopharyngeal carriage of Streptococcus pneumoniae
by Steens, Anneke and Milhano, Natacha Alexandra da Fonseca and Aaberge, Ingeborg Sundsvalen and Vestrheim, Didrik F
ISSN 2167-8359, 2016
Background. As a standard method for pneumococcal carriage studies, the World Health Organization recommends nasopharyngeal swabs be transported and stored at... 
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9. Full Text PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal
by Santos-Silva, Maria Margarida and Melo, Pedro and Santos, Patrícia Tavares and Santos, Nuno and Santos, Ana Sofia and Antunes, Sandra and Duarte, Luís Raposo and Ferrolho, Joana and Milhano, Natacha and Domingos, Ana
Molecular and Cellular Probes, ISSN 0890-8508, 02/2017, Volume 31, pp. 42 - 45
The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of (Iberian lynx), one of the most endangered... 
Iberian lynx habitat | Ticks | Candidatus midichloria mitochondrii | Hyalomma lusitanicum | Anaplasma platys | Coxiella burnetii | Tick-borne agents | ACARI | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | GENE-SEQUENCES | COXIELLA-BURNETII | ANAPLASMA-PLATYS | CELL BIOLOGY | EHRLICHIA | BABESIA-CANIS-VOGELI | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | MOLECULAR-DETECTION | INFECTION | RHIPICEPHALUS | PATHOGENS | Geography | Ticks - genetics | Animals | Lynx - parasitology | Portugal | Polymerase Chain Reaction - methods | Analysis | Lynx | Extinction (Biology) | Tick-borne diseases
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10. Full Text Rickettsia lusitaniae sp. nov. isolated from the soft tick Ornithodoros erraticus (Acarina: Argasidae)
by Milhano, Natacha and Palma, Mariana and Marcili, Arlei and Núncio, Maria Sofia and de Carvalho, Isabel Lopes and de Sousa, Rita
Comparative Immunology, Microbiology and Infectious Diseases, ISSN 0147-9571, 2014, Volume 37, Issue 3, pp. 189 - 193
Abstract In this study a novel Rickettsia from the spotted fever group, isolated from Ornithodoros erraticus soft ticks collected from pigpens in the south of... 
Infectious Disease | Allergy and Immunology | Rickettsia lusitaniae sp. nov | Pig pens | Soft tick | Ornithodoros erraticus | Portugal | SWINE-FEVER VIRUS | HOOGSTRAALII | HUMANS | MICROBIOLOGY | CARIOS-CAPENSIS ACARI | IMMUNOLOGY | HELVETICA | AFRICA | BORRELIA-HISPANICA | Rickettsia lusitaniae sp nov | VETERINARY SCIENCES | RELAPSING FEVER | IXODOIDEA | Genes, Bacterial | Multigene Family | Rickettsia - genetics | Cercopithecus aethiops | Phylogeny | Rickettsia - isolation & purification | Rickettsia - classification | Animals | Swine | Genetic Speciation | Ornithodoros - microbiology | Female | Vero Cells
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11. Rickettsia massiliae and Rickettsia conorii Israeli Spotted Fever Strain Differentially Regulate Endothelial Cell Responses: e0138830
by Bechelli, Jeremy and Smalley, Claire and Milhano, Natacha and Walker, David H and Fang, Rong
PLoS ONE, 09/2015, Volume 10, Issue 9
Rickettsiae primarily target microvascular endothelial cells. However, it remains elusive how endothelial cell responses to rickettsiae play a role in the... 
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12. Full Text Quantitative study of Rickettsia massiliae in Rhipicephalus sanguineus organs
by Milhano, Natacha and Popov, Vsevolod and Vilhena, Manuela and Bouyer, Donald H and de Sousa, Rita and Walker, David H
Ticks and Tick-borne Diseases, ISSN 1877-959X, 10/2014, Volume 5, Issue 6, pp. 709 - 714
, belonging to the spotted fever group of , is a human pathogen causing a similar course of disease to that caused by , the originally recognized etiologic... 
Rickettsia massiliae | Quantitative real-time PCR | Electron microscopy | Rhipicephalus sanguineus | Ticks | PORTUGAL | INFECTIOUS DISEASES | FEVER GROUP RICKETTSIAE | ACARI | HUMANS | MICROBIOLOGY | IXODIDAE | SPOTTED-FEVER | INFECTION | PATIENT | PARASITOLOGY | SPAIN | Rhipicephalus sanguineus - physiology | Animals | Guinea Pigs | Rickettsia - genetics | Rhipicephalus sanguineus - ultrastructure | Female | Male | Salivary Glands - microbiology | Rhipicephalus sanguineus - microbiology | Rickettsia - isolation & purification
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13. Full Text Coinfections of Rickettsia slovaca and Rickettsia helvetica with Borrelia lusitaniae in ticks collected in a Safari Park, Portugal
by Milhano, Natacha and Carvalho, Isabel Lopes de and Alves, Ana Sofia and Arroube, Sofia and Soares, Jorge and Rodriguez, Pablo and Carolino, Manuela and Núncio, Maria Sofia and Piesman, Joseph and de Sousa, Rita
Ticks and Tick-borne Diseases, ISSN 1877-959X, 2010, Volume 1, Issue 4, pp. 172 - 177
and bacteria are the most important tick-borne agents causing disease in Portugal. Identification and characterization of these circulating agents, mainly in... 
Safari park | Borrelia | Rickettsia | Portugal | Ticks | INFECTIOUS DISEASES | BURGDORFERI SENSU-LATO | MICROBIOLOGY | IDENTIFICATION | MONACENSIS | SPOTTED-FEVER GROUP | HUMAN GRANULOCYTIC EHRLICHIOSIS | IXODES-RICINUS TICKS | SP-NOV | LYME-DISEASE | MAINLAND PORTUGAL | PARASITOLOGY | ANAPLASMA-PHAGOCYTOPHILUM | Animals | Coinfection | Borrelia Infections - epidemiology | Borrelia - isolation & purification | Female | Male | Arachnid Vectors - microbiology | Rickettsia - isolation & purification | Rickettsia Infections - epidemiology | Ticks - microbiology
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14. Full Text African Tick Bite Fever in a Portuguese Traveler Returning From South Africa
by Gonzalez, Filipe André and Martins, Joana and Coelho, Margarida and Amaro, Mário and Milhano, Natacha and Santos, Ana Sofia and de Sousa, Rita
Infectious Diseases in Clinical Practice, ISSN 1056-9103, 03/2012, Volume 20, Issue 2, pp. 150 - 151
ABSTRACTWe report the first imported case of African tick bite fever in a Portuguese traveler who returned from South Africa. The clinical diagnosis was... 
Rickettsia africae | traveler | Portugal | African tick bite fever
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15. Full Text Detection of Borrelia lusitaniae, Rickettsia sp. IRS3, Rickettsia monacensis, and Anaplasma phagocytophilum in Ixodes ricinus collected in Madeira Island, Portugal
by Lopes de Carvalho, Isabel and Milhano, Natacha and Santos, Ana Sofia and Almeida, Victor and Barros, Silvia C and De Sousa, Rita and Nuncio, Maria Sofia
Vector-Borne and Zoonotic Diseases, ISSN 1530-3667, 12/2008, Volume 8, Issue 6, p. 575
Polymerase chain reaction | Usage | Borrelia | Ticks | Distribution | Genetic aspects | Research | Health aspects
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16. Full Text Detection of Borrelia lusitaniae, Rickettsia sp. IRS3, Rickettsia monacensis, and Anaplasma phagocytophilum in Ixodes ricinus collected in Madeira Island, Portugal
by Carvalho, Isabel Lopes De and Milhano, Natacha and Santos, Ana Sofia and Almeida, Victor and Barros, Silvia C and Sousa, Rita De and Núncio, Maria Sofia
Vector-Borne and Zoonotic Diseases, ISSN 1530-3667, 08/2008, Volume 8, Issue 4, pp. 575 - 579
A total of 300 Ixodes ricinus ticks were tested by polymerase chain reaction (PCR) for the presence of Borrelia spp., Rickettsia spp., and Anaplasma... 
Anaplasma phagocytophilum | B. lusitaniae | Ixodes ricinus | Rickettsia | Madeira | INFECTIOUS DISEASES | BURGDORFERI SENSU-LATO | IDENTIFICATION | Anaplasma phagocytophilmn | PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH | AMPLIFICATION | EHRLICHIA | SEQUENCE ALIGNMENT | DNA | TICKS | DISEASE | GENES | Animals | Ixodes - microbiology | Anaplasma phagocytophilum - isolation & purification | Borrelia - isolation & purification | Rickettsia - isolation & purification | Portugal | Borrelia - classification | Rickettsia - classification
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