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1. Full Text The Altered Supramolecular Structure of Dopamine D2 Receptors in Disc1-deficient Mice
by Onishi, Taichi and Sakamoto, Hirokazu and Namiki, Shigeyuki and Hirose, Kenzo
Scientific Reports, ISSN 2045-2322, 12/2018, Volume 8, Issue 1, pp. 1692 - 8
Disc1 is a susceptibility gene for psychiatric disorders including schizophrenia. It has been suggested that excess transmission through dopamine type 2... 
Animals | Nerve Tissue Proteins - deficiency | Protein Multimerization | Receptors, Dopamine D2 - chemistry | Protein Conformation | Mice | Receptors, Dopamine D2 - metabolism | Microscopy, Fluorescence | Nucleus Accumbens - pathology | Dopamine | Mental disorders | Exons | Spatial discrimination | Schizophrenia | Clozapine | Spiny neurons | Nucleus accumbens | Dendritic spines | Spatial distribution | Disease transmission | Rodents | Neostriatum | Dendrites | Dopamine D2 receptors | Disc1 protein | Dopamine receptors
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2. Full Text Synaptic weight set by Munc13-1 supramolecular assemblies
by Sakamoto, Hirokazu and Ariyoshi, Tetsuroh and Kimpara, Naoya and Sugao, Kohtaroh and Taiko, Isamu and Takikawa, Kenji and Asanuma, Daisuke and Namiki, Shigeyuki and Hirose, Kenzo
Nature Neuroscience, ISSN 1097-6256, 01/2018, Volume 21, Issue 1, pp. 41 - 55
The weight of synaptic connections, which is controlled not only postsynaptically but also presynaptically, is a key determinant in neuronal network dynamics.... 
HETEROGENEITY | ORGANIZATION | TRANSMISSION | PROBABILITY FLUCTUATION ANALYSIS | NEUROTRANSMITTER RELEASE | SYNAPSES | MULTIVESICULAR RELEASE | POOL | FACILITATION | NEUROSCIENCES | ACZONIN/PICCOLO | RNA, Small Interfering - genetics | Synaptic Vesicles - metabolism | Synaptic Transmission - physiology | Calcium - metabolism | Embryo, Mammalian | Syntaxin 1 - metabolism | Exocytosis - physiology | GTP-Binding Proteins - genetics | Neurons - ultrastructure | Synapses - metabolism | Cytoskeletal Proteins - metabolism | Neurons - metabolism | Nerve Tissue Proteins - immunology | Vesicular Glutamate Transport Protein 1 - metabolism | Cells, Cultured | Rats | Neuropeptides - metabolism | Synapses - ultrastructure | Hippocampus - cytology | Nerve Tissue Proteins - genetics | Rats, Sprague-Dawley | Nerve Tissue Proteins - metabolism | Animals | Presynaptic Terminals - metabolism | Glutamic Acid - metabolism | Adaptor Proteins, Signal Transducing - metabolism | GTP-Binding Proteins - metabolism | RNA, Small Interfering - metabolism | Molecular neurobiology | Neural circuitry | Brain research | Synaptic vesicles | Physiological aspects | Neural transmission | Research | Neurophysiology | Image resolution | Synaptic strength | SNAP receptors | Exocytosis | Biological activity | Proteins | Syntaxin | Robustness (mathematics) | Syntaxin 1 | Glutamatergic transmission | Assemblies | Ethylmaleimide | Synapses | N-Ethylmaleimide
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3. Full Text Acidic‐pH‐Activatable Fluorescence Probes for Visualizing Exocytosis Dynamics
by Asanuma, Daisuke and Takaoka, Yousuke and Namiki, Shigeyuki and Takikawa, Kenji and Kamiya, Mako and Nagano, Tetsuo and Urano, Yasuteru and Hirose, Kenzo
Angewandte Chemie International Edition, ISSN 1433-7851, 06/2014, Volume 53, Issue 24, pp. 6085 - 6089
Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding of secretion phenomena, but current approaches have serious... 
exocytosis | acidity | fluorescent probes | spatiotemporal dynamics | vesicular imaging | CELLS | FUSION | SECRETION | CHEMISTRY, MULTIDISCIPLINARY | Fluorescent Dyes - chemistry | Exocytosis - physiology | Hydrogen-Ion Concentration | Fluorescence | Hydrogen-ion concentration | Investigations | Visualization | Cellular | Dynamic tests | Dynamics | Imaging | Tools | Stimulation | Secretions
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4. Full Text Imaging extrasynaptic glutamate dynamics in the brain
by Yohei Okubo and Hiroshi Sekiya and Shigeyuki Namiki and Hirokazu Sakamoto and Sho Iinuma and Miwako Yamasaki and Masahiko Watanabe and Kenzo Hirose and Masamitsu Iino and Roberto Malinow
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 4/2010, Volume 107, Issue 14, pp. 6526 - 6531
Glutamate is the major neurotransmitter in the brain, mediating point-to-point transmission across the synaptic cleft in excitatory synapses. Using a glutamate... 
Glutamate receptors | Brain | Neuroscience | Amino acid transport system XAG | Synaptic transmission | Neurons | Purkinje cells | Imaging | Fluorescence | Synapses | Two-photon microscopy | In vivo | Synapse | Fluorescence imaging | Spillover | spillover | CEREBELLAR PURKINJE-CELLS | RAT | fluorescence imaging | MULTIDISCIPLINARY SCIENCES | SYNAPTIC-TRANSMISSION | RECEPTOR | synapse | ASTROCYTES | PARALLEL FIBER SYNAPSES | DENDRITIC SPINES | OLFACTORY-BULB | CALCIUM-RELEASE | two-photon microscopy | in vivo | Mice, Inbred C57BL | Rats | Rats, Sprague-Dawley | Microscopy, Immunoelectron | Brain - metabolism | Extracellular Space - metabolism | Animals | Synapses - metabolism | Brain Chemistry | Glutamic Acid - metabolism | Mice | Synapses - chemistry | Glutamic Acid - analysis | Physiological aspects | Research | Glutamate | Fluorescence microscopy | Methods | Biological Sciences
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5. Full Text Adiponectin and AdipoR1 regulate PGC-1α and mitochondria by Ca 2+ and AMPK/SIRT1
by Iwabu, Masato and Yamauchi, Toshimasa and Okada-Iwabu, Miki and Sato, Koji and Nakagawa, Tatsuro and Funata, Masaaki and Yamaguchi, Mamiko and Namiki, Shigeyuki and Nakayama, Ryo and Tabata, Mitsuhisa and Ogata, Hitomi and Kubota, Naoto and Takamoto, Iseki and Hayashi, Yukiko K and Yamauchi, Naoko and Waki, Hironori and Fukayama, Masashi and Nishino, Ichizo and Tokuyama, Kumpei and Ueki, Kohjiro and Oike, Yuichi and Ishii, Satoshi and Hirose, Kenzo and Shimizu, Takao and Touhara, Kazushige and Kadowaki, Takashi
Nature, ISSN 0028-0836, 04/2010, Volume 464, Issue 7293, pp. 1313 - 1319
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6. Full Text Enzymatic production of RNAi libraries from cDNAs
by Namiki, Shigeyuki and Tanabe, Mao and Sugao, Kohtaroh and Shirane, Daisuke and Hirose, Kenzo and Iino, Masamitsu
Nature Genetics, ISSN 1061-4036, 02/2004, Volume 36, Issue 2, pp. 190 - 196
RNA interference (RNAi) induced by small interfering (siRNA) or short hairpin RNA (shRNA) is an important research approach in mammalian genetics. Here we... 
STEM-CELLS | INOSITOL 1,4,5-TRISPHOSPHATE | CAENORHABDITIS-ELEGANS | SEQUENCE | GENETICS & HEREDITY | GENE-EXPRESSION | SHORT-INTERFERING RNAS | RECEPTOR | MAMMALIAN-CELLS | MESSENGER | GENOME | Deoxyribonuclease I - metabolism | DNA, Complementary - physiology | Gene Library | Jurkat Cells | Plasmids | Humans | RNA, Small Interfering - physiology | DNA-Binding Proteins - genetics | Genetic Vectors | RNA Interference - physiology | Genes, Reporter | Usage | Genomic libraries | Antisense DNA | Design and construction | Analysis | siRNA
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7. Full Text High‐Throughput Development of a Hybrid‐Type Fluorescent Glutamate Sensor for Analysis of Synaptic Transmission
by Takikawa, Kenji and Asanuma, Daisuke and Namiki, Shigeyuki and Sakamoto, Hirokazu and Ariyoshi, Tetsuro and Kimpara, Naoya and Hirose, Kenzo
Angewandte Chemie International Edition, ISSN 1433-7851, 12/2014, Volume 53, Issue 49, pp. 13439 - 13443
Fluorescent sensors are powerful tools for visualizing cellular molecular dynamics. We present a high‐throughput screening system, designated hybrid‐type... 
neurotransmitters | fluorescent probes | high‐throughput screening | High-throughput screening | Neurotransmitters | Fluorescent probes | TRYPTOPHAN | PROTEIN | ORGANIC-DYES | high-throughput screening | RECEPTOR | CHEMISTRY, MULTIDISCIPLINARY | KNOWLEDGE-BASED DESIGN | PROBES | DYNAMICS | LIGAND-BINDING DOMAIN | BIOSENSOR | BRAIN | Optical Imaging | Animals | High-Throughput Screening Assays | Synapses - metabolism | Humans | Fluorescent Dyes - analysis | Fluorescent Dyes - metabolism | Models, Molecular | Glutamic Acid - metabolism | Receptors, AMPA - metabolism | Synaptic Transmission | Glutamic Acid - analysis | Fluorescence | Cysteine | Glutamate | Protein binding | Rodents | Sensors | Heterogeneity | Space probes | Dynamics | Glutamates | Dynamical systems | Chemical compounds | Synapses
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8. Full Text Adiponectin and AdipoR1 regulate PGC-1 alpha and mitochondria by Ca2+ and AMPK/SIRT1
by Iwabu, M and Yamauchi, T and Okada-Iwabu, M and Sato, K and Nakagawa, T and Funata, M and Yamaguchi, M and Namiki, S and Nakayama, R and Tabata, M and Ogata, H and Kubota, N and Takamoto, I and Hayashi, YK and Yamauchi, N and Waki, H and Fukayama, M and Nishino, I and Tokuyama, K and Ueki, K and Oike, Y and Ishii, S and Hirose, K and Shimizu, T and Touhara, K and Kadowaki, T
NATURE, ISSN 0028-0836, 04/2010, Volume 464, Issue 7293, pp. 1313 - 1319
Adiponectin is an anti-diabetic adipokine. Its receptors possess a seven-transmembrane topology with the amino terminus located intracellularly, which is the... 
SKELETAL-MUSCLE | OBESITY | ACTIVATED PROTEIN-KINASE | FATTY-ACID OXIDATION | PHOSPHORYLATION | CLONING | INSULIN-RESISTANCE | MULTIDISCIPLINARY SCIENCES | DISEASE | RECEPTOR ACTIVATION | MUSCLE FIBER-TYPE
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9. Full Text Cell Surface-Anchored Fluorescent Aptamer Sensor Enables Imaging of Chemical Transmitter Dynamics
by Tokunaga, Takeshi and Namiki, Shigeyuki and Yamada, Katsuhiro and Imaishi, Takahiro and Nonaka, Hiroshi and Hirose, Kenzo and Sando, Shinsuke
Journal of the American Chemical Society, ISSN 0002-7863, 06/2012, Volume 134, Issue 23, pp. 9561 - 9564
A fluorescent aptamer sensor was applied to the analysis of extracellular chemical transmitter dynamics. We utilized a tocopherol-labeled aptamer, which... 
RECOGNITION | SYNAPTIC-TRANSMISSION | DNA APTAMER | ATP | ASTROCYTES | CHEMISTRY, MULTIDISCIPLINARY | BRAIN | Astrocytes - cytology | Fluorescent Dyes - chemistry | Brain - cytology | Humans | Cells, Cultured | Aptamers, Nucleotide - chemistry | Models, Molecular | Rats | Adenine - analysis | Animals | Microscopy, Fluorescence - methods | Biosensing Techniques - methods | Adenine - metabolism | HeLa Cells | Tocopherols - chemistry | Calcium Signaling | Astrocytes - metabolism | Usage | Neurotransmitters | Astrocytes | Brain mapping | Molecular diagnostic techniques | Adenine | Physiological aspects | Brain cells | Chemical properties | Methods
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10. Spatiotemporal Control of Receptor Tyrosine Kinase Activity by Caged Ligands
by Masayuki Isa and Shigeyuki Namiki and Daisuke Asanuma and Kenzo Hirose
Chemistry Letters, ISSN 0366-7022, 02/2015, Volume 44, Issue 2, p. 150
  We developed a technique for spatiotemporally precise control of downstream signaling of receptor tyrosine kinase by ultraviolet light irradiation of caged... 
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11. Spatiotemporal Control of Receptor Tyrosine Kinase Activity by Caged Ligands
by Isa, Masayuki and Namiki, Shigeyuki and Asanuma, Daisuke and Hirose, Kenzo
Chemistry Letters, ISSN 0366-7022, 01/2015, Volume 44, Issue 2, pp. 150 - 150
We developed a technique for spatiotemporally precise control of downstream signaling of receptor tyrosine kinase by ultraviolet light irradiation of caged... 
Tyrosine | Receptors | Dynamic tests | Light irradiation | Calcium | Dynamics | Ligands | Kinases
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12. Full Text Serum luteinizing hormone concentration is significantly associated with recovery of urinary function after radical prostatectomy
by Namiki, Shunichi and Mitsuzuka, Koji and Kaiho, Yasuhiro and Yamada, Shigeyuki and Adachi, Hisanobu and Yamashita, Shinichi and Saito, Hideo and Ito, Akihiro and Nakagawa, Haruo and Takegami, Misa and Arai, Yoichi
BJU International, ISSN 1464-4096, 03/2016, Volume 117, Issue 3, pp. 450 - 455
Objective To perform a longitudinal investigation of the correlation between functional recovery and sex hormone concentrations after radical prostatectomy... 
prostate cancer | radical prostatectomy | hypogonadism | testosterone | luteinizing hormone | SYMPTOMS | CANCER | INDEX COMPOSITE | TRACT | MEN | UROLOGY & NEPHROLOGY | CONTINENCE | QUALITY-OF-LIFE | RECEPTORS | EXPRESSION | Prostatectomy - methods | Prospective Studies | Prostatic Neoplasms - surgery | Humans | Middle Aged | Male | Prostatic Neoplasms - physiopathology | Luteinizing Hormone - metabolism | Urinary Retention - blood | Preoperative Care | Recovery of Function | Urinary Retention - physiopathology | Urinary Retention - surgery | Testosterone - metabolism | Postoperative Care | Prostatic Neoplasms - blood | Quality of Life | Aged | Testosterone | Pituitary hormones | Analysis | Surgery | Luteinizing hormone | Glycoproteins | Prostate cancer
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13. Full Text Long‐term quality of life after radical prostatectomy: 8‐Year longitudinal study in Japan
by Namiki, Shunichi and Kaiho, Yasuhiro and Mitsuzuka, Koji and Saito, Hideo and Yamada, Shigeyuki and Nakagawa, Haruo and Ito, Akihiro and Arai, Yoichi
International Journal of Urology, ISSN 0919-8172, 12/2014, Volume 21, Issue 12, pp. 1220 - 1226
Objectives To assess long‐term health‐related quality of life in patients undergoing radical prostatectomy. Methods A total of 120 patients with at least 5... 
questionnaires | prostate cancer | radical prostatectomy | quality of life | Radical prostatectomy | Prostate cancer | Questionnaires | Quality of life | SYMPTOMS | SEXUAL FUNCTION | CANCER | IMPACT | THERAPY | UROLOGY & NEPHROLOGY | CONTINENCE | RETROPUBIC PROSTATECTOMY | OUTCOMES | BRACHYTHERAPY | VALIDITY | Follow-Up Studies | Prostatic Neoplasms - surgery | Humans | Japan | Middle Aged | Postoperative Period | Male | Treatment Outcome | Prostatic Neoplasms - psychology | Time Factors | Quality of Life | Prostatectomy | Surveys and Questionnaires | Aged | Health Status | Surgery
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14. Full Text NO signalling decodes frequency of neuronal activity and generates synapse-specific plasticity in mouse cerebellum
by Shigeyuki Namiki and Sho Kakizawa and Kenzo Hirose and Masamitsu Iino
The Journal of Physiology, ISSN 0022-3751, 08/2005, Volume 566, Issue 3, pp. 849 - 863
Nitric oxide (NO) is an intercellular messenger regulating neuronal functions. To visualize NO signalling in the brain, we generated a novel fluorescent NO... 
PURKINJE-CELLS | SINDBIS VIRUS | ENDOGENOUS CANNABINOIDS | PHYSIOLOGY | NITRIC-OXIDE SYNTHASE | LONG-TERM DEPRESSION | RETROGRADE INHIBITION | METABOTROPIC GLUTAMATE RECEPTORS | CENTRAL-NERVOUS-SYSTEM | SOLUBLE GUANYLATE-CYCLASE | GRANULE CELL | Action Potentials - physiology | Animals | Neuronal Plasticity - physiology | Synaptic Transmission - physiology | Synapses - physiology | Mice, Inbred C57BL | Mice | Cerebellum - physiology | In Vitro Techniques | Nitric Oxide - metabolism | Nerve Net - physiology | Tissue, System and Organ Physiology
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15. Full Text Spatiotemporal analysis of glutamatergic neurotransmission
by Sakamoto, Hirokazu and Namiki, Shigeyuki and Hirose, Kenzo
Neuroscience Research, ISSN 0168-0102, 09/2011, Volume 71, pp. e94 - e94
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16. Full Text MP15-10 LONG-TERM QUALITY OF LIFE AFTER RADICAL PROSTATECTOMY: 8-YEAR LONGITUDINAL STUDY
by Namiki, Shunichi and Mitsuzuka, Koji and Kaiho, Yasuhiro and Ito, Akihiro and Yamada, Shigeyuki and Saito, Hideo and Nakagawa, Haruo and Arai, Yoichi
Journal of Urology, The, ISSN 0022-5347, 2014, Volume 191, Issue 4, pp. e148 - e149
Urology
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17. Full Text Spatiotemporal Control of Receptor Tyrosine Kinase Activity by Caged Ligands
by Isa, Masayuki and Namiki, Shigeyuki and Asanuma, Daisuke and Hirose, Kenzo
Chemistry Letters, ISSN 0366-7022, 2/2015, Volume 44, Issue 2, pp. 150 - 151
We developed a technique for spatiotemporally precise control of downstream signaling of receptor tyrosine kinase by ultraviolet light irradiation of caged... 
AXON | NEURONS | NEUROTROPHINS | CHEMISTRY, MULTIDISCIPLINARY
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18. Full Text P1-1-4 Functional imaging of molecular architecture at a presynaptic active zone
by Hirokazu Sakamoto and Shigeyuki Namiki and Kenzo Hirose and Tokyo Univ. Sch. Med and Dept. Neurobiol
Journal of Pharmacological Sciences, ISSN 1347-8613, 2012, Volume 118, Issue suppl, pp. 134 - 134
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19. Full Text Low‐dose maintenance gemcitabine‐carboplatin chemotherapy could be an alternative to continuous standard chemotherapy for patients with metastatic urothelial carcinoma
by Mitsuzuka, Koji and Yamashita, Shinichi and Namiki, Shunichi and Yamada, Shigeyuki and Sato, Ko and Saito, Hideo and Kaiho, Yasuhiro and Ito, Akihiro and Nakagawa, Haruo and Arai, Yoichi
International Journal of Urology, ISSN 0919-8172, 11/2014, Volume 21, Issue 11, pp. 1114 - 1119
Objectives To examine whether low‐dose maintenance gemcitabine‐carboplatin chemotherapy is beneficial for patients with metastatic urothelial carcinoma.... 
adverse event | low‐dose maintenance chemotherapy | urothelial carcinoma | carboplatin | cisplatin‐unfit | cisplatin-unfit | low-dose maintenance chemotherapy | BLADDER-CANCER | DOXORUBICIN | METHOTREXATE | VINBLASTINE | PACLITAXEL | CISPLATIN-BASED CHEMOTHERAPY | PHASE-II TRIAL | PLATINUM-CONTAINING REGIMEN | TRANSITIONAL-CELL CARCINOMA | URINARY-TRACT | UROLOGY & NEPHROLOGY | Deoxycytidine - administration & dosage | Humans | Middle Aged | Antineoplastic Combined Chemotherapy Protocols | Carboplatin - administration & dosage | Male | Antineoplastic Agents - administration & dosage | Carboplatin - adverse effects | Urologic Neoplasms - drug therapy | Antineoplastic Agents - adverse effects | Carcinoma, Transitional Cell - drug therapy | Deoxycytidine - adverse effects | Female | Aged | Retrospective Studies | Deoxycytidine - analogs & derivatives | Antimitotic agents | Care and treatment | Chemotherapy | Carcinoma | Dosage and administration | Metastasis | Antineoplastic agents | Standards | Cancer
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20. Full Text Artificial Control of Subtype-Specific Platelet-Derived Growth Factor– Receptor Signaling
by Isa, Masayuki and Ohta, Yusaku and Namiki, Shigeyuki and Hirose, Kenzo
Journal of Pharmacological Sciences, ISSN 1347-8613, 2009, Volume 111, Issue 3, pp. 312 - 316
Platelet-derived growth factor (PDGF) signaling controls various physiological functions via two receptor subtypes: PDGF receptor (PDGFR) and PDGFR .... 
platelet-derived growth factor receptor | chimeric receptor | Platelet-derived growth factor receptor, chimeric receptor | CDC42 | TRANSDUCTION | PHARMACOLOGY & PHARMACY | CELL-MIGRATION | Amino Acid Sequence | Humans | Mutant Chimeric Proteins - genetics | Molecular Sequence Data | Receptors, Platelet-Derived Growth Factor - genetics | cdc42 GTP-Binding Protein - metabolism | Fluoresceins | Cell Movement - drug effects | Signal Transduction - drug effects | Calcium Signaling - drug effects | cdc42 GTP-Binding Protein - genetics | Ligands | Receptors, Platelet-Derived Growth Factor - drug effects | Mutant Chimeric Proteins - drug effects
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