Science, ISSN 0036-8075, 02/2017, Volume 355, Issue 6324, pp. 511 - 515
Sleep is an essential process that supports learning and memory by acting on synapses through poorly understood molecular mechanisms. Using biochemistry,...
ACTIVATION | PROTEIN | MEMORY | DEPRIVATION | PERSISTENCE | MULTIDISCIPLINARY SCIENCES | METABOTROPIC GLUTAMATE RECEPTORS | MICE | WAKE | SYNAPTIC PLASTICITY | MODULATION | Homer Scaffolding Proteins - genetics | Norepinephrine - pharmacology | Wakefulness - drug effects | Synapses - physiology | Cell Count | Cells, Cultured | Homeostasis | Rats | Prosencephalon - physiology | Receptors, Metabotropic Glutamate - metabolism | Adenosine - pharmacology | Receptor, Metabotropic Glutamate 5 - metabolism | Rats, Sprague-Dawley | Sleep - drug effects | Learning - physiology | Homer Scaffolding Proteins - physiology | Animals | Neurons - ultrastructure | Prosencephalon - cytology | Neurons - physiology | Cognition - physiology | Wakefulness - physiology | Mice | Sleep - physiology | Brain | Head | Spine | Memory | Data processing | Electron microscopy | Neuromodulation | Learning | Receptors | Sleep | Consolidation | Rodents | Arousal | Proteomics | Scaling | Noradrenaline | Synapses | Adenosines | Genes | Glutamates
ACTIVATION | PROTEIN | MEMORY | DEPRIVATION | PERSISTENCE | MULTIDISCIPLINARY SCIENCES | METABOTROPIC GLUTAMATE RECEPTORS | MICE | WAKE | SYNAPTIC PLASTICITY | MODULATION | Homer Scaffolding Proteins - genetics | Norepinephrine - pharmacology | Wakefulness - drug effects | Synapses - physiology | Cell Count | Cells, Cultured | Homeostasis | Rats | Prosencephalon - physiology | Receptors, Metabotropic Glutamate - metabolism | Adenosine - pharmacology | Receptor, Metabotropic Glutamate 5 - metabolism | Rats, Sprague-Dawley | Sleep - drug effects | Learning - physiology | Homer Scaffolding Proteins - physiology | Animals | Neurons - ultrastructure | Prosencephalon - cytology | Neurons - physiology | Cognition - physiology | Wakefulness - physiology | Mice | Sleep - physiology | Brain | Head | Spine | Memory | Data processing | Electron microscopy | Neuromodulation | Learning | Receptors | Sleep | Consolidation | Rodents | Arousal | Proteomics | Scaling | Noradrenaline | Synapses | Adenosines | Genes | Glutamates
Journal Article
eLife, ISSN 2050-084X, 11/2017, Volume 6
We previously reported that Parkinson's disease (PD) kinase LRRK2 phosphorylates a subset of Rab GTPases on a conserved residue in their switch-II domains...
KINASE 2 LRRK2 | PATHWAYS | RECRUITMENT | STRUCTURAL BASIS | QUANTIFICATION | DISEASE | BIOLOGY | POSTTRANSLATIONAL MODIFICATIONS | MUTATIONS | PROTEINS | RILP | rab GTP-Binding Proteins - metabolism | Phosphorylation | Fibroblasts - physiology | Humans | Cells, Cultured | Low Density Lipoprotein Receptor-Related Protein-1 - metabolism | Cilia - metabolism | Gene Knock-In Techniques | Protein Interaction Mapping | Organelle Biogenesis | Animals | Carrier Proteins - metabolism | Mass Spectrometry | Proteomics | Protein Binding | Mice | Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 - metabolism | Hypothesis testing | Enzymes | Starvation | Parkinson's disease | Peptides | Neurodegenerative diseases | Pathogenesis | Mass spectroscopy | Biochemistry | Kinases | Proteins | Signal transduction | Rab3A protein | Rab protein | Fibroblasts | Scientific imaging | Mutation | LRRK2 protein | Protein transport | Mass spectrometry | Movement disorders | Guanosinetriphosphatase | Cilia
KINASE 2 LRRK2 | PATHWAYS | RECRUITMENT | STRUCTURAL BASIS | QUANTIFICATION | DISEASE | BIOLOGY | POSTTRANSLATIONAL MODIFICATIONS | MUTATIONS | PROTEINS | RILP | rab GTP-Binding Proteins - metabolism | Phosphorylation | Fibroblasts - physiology | Humans | Cells, Cultured | Low Density Lipoprotein Receptor-Related Protein-1 - metabolism | Cilia - metabolism | Gene Knock-In Techniques | Protein Interaction Mapping | Organelle Biogenesis | Animals | Carrier Proteins - metabolism | Mass Spectrometry | Proteomics | Protein Binding | Mice | Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 - metabolism | Hypothesis testing | Enzymes | Starvation | Parkinson's disease | Peptides | Neurodegenerative diseases | Pathogenesis | Mass spectroscopy | Biochemistry | Kinases | Proteins | Signal transduction | Rab3A protein | Rab protein | Fibroblasts | Scientific imaging | Mutation | LRRK2 protein | Protein transport | Mass spectrometry | Movement disorders | Guanosinetriphosphatase | Cilia
Journal Article
Biochemical Journal, ISSN 0264-6021, 10/2019, Volume 476, Issue 20, pp. 3081 - 3107
Abstract The serum- and glucocorticoid-regulated kinase (SGK) isoforms contribute resistance to cancer therapies targeting the PI3K pathway. SGKs are...
phosphoproteomics | Signaling | syntaxins | s | Post-Translational Modifications | SGK3 | Akt | phosphoinositide 3-kinase | Cancer
phosphoproteomics | Signaling | syntaxins | s | Post-Translational Modifications | SGK3 | Akt | phosphoinositide 3-kinase | Cancer
Journal Article
Molecular Biology of the Cell, ISSN 1059-1524, 06/2017, Volume 28, Issue 11, pp. 1489 - 1506
Members of the adenine nucleotide translocase (ANT) family exchange ADP for ATP across the mitochondrial inner membrane, an activity that is essential for...
PERMEABILITY TRANSITION PORE | CYTOCHROME-C-OXIDASE | OXIDATIVE STRESS | ATP SYNTHASE | MITOCHONDRIAL ADP/ATP CARRIER | CULTURED HUMAN-CELLS | TISSUE DISTRIBUTION | KINETIC-PROPERTIES | SACCHAROMYCES-CEREVISIAE | BACTERIAL EXPRESSION | CELL BIOLOGY | Humans | Oxidative Phosphorylation | Mitochondria - metabolism | Structure-Activity Relationship | Mitochondrial ADP, ATP Translocases - metabolism | Mitochondrial Membranes - metabolism | Mitochondrial ADP, ATP Translocases - genetics | Protein Isoforms - metabolism | Adenosine Triphosphate - metabolism | HEK293 Cells | Adenosine Diphosphate - metabolism | HeLa Cells | Apoptosis
PERMEABILITY TRANSITION PORE | CYTOCHROME-C-OXIDASE | OXIDATIVE STRESS | ATP SYNTHASE | MITOCHONDRIAL ADP/ATP CARRIER | CULTURED HUMAN-CELLS | TISSUE DISTRIBUTION | KINETIC-PROPERTIES | SACCHAROMYCES-CEREVISIAE | BACTERIAL EXPRESSION | CELL BIOLOGY | Humans | Oxidative Phosphorylation | Mitochondria - metabolism | Structure-Activity Relationship | Mitochondrial ADP, ATP Translocases - metabolism | Mitochondrial Membranes - metabolism | Mitochondrial ADP, ATP Translocases - genetics | Protein Isoforms - metabolism | Adenosine Triphosphate - metabolism | HEK293 Cells | Adenosine Diphosphate - metabolism | HeLa Cells | Apoptosis
Journal Article
Nature Communications, ISSN 2041-1723, 2014, Volume 5, Issue 1, pp. 4961 - 4961
The PIK3CA gene is frequently mutated in human cancers. Here we carry out a SILAC-based quantitative phosphoproteomic analysis using isogenic knockin cell...
BREAST-CANCER | GROWTH-FACTOR RECEPTOR | PHOSPHORYLATED PEPTIDES | NOVO PYRIMIDINE SYNTHESIS | PROTEIN-KINASE-B | PHOSPHATIDYLINOSITOL 3-KINASE | CELL-MIGRATION | MULTIDISCIPLINARY SCIENCES | ATP-CITRATE LYASE | HIGHLY SELECTIVE ENRICHMENT | TITANIUM-DIOXIDE | RNA, Small Interfering - genetics | Immunoprecipitation | Mutagenesis, Site-Directed | Humans | Immunoblotting | DNA Primers - genetics | Signal Transduction - genetics | Cortactin - metabolism | Phosphoproteins - genetics | Mutation - genetics | Reverse Transcriptase Polymerase Chain Reaction | Gene Knock-In Techniques | Phosphatidylinositol 3-Kinases - genetics | Tandem Mass Spectrometry | Class I Phosphatidylinositol 3-Kinases | Fluorescent Antibody Technique | Proteomics | Chromatography, Liquid | Signal Transduction - physiology | Proto-Oncogene Proteins c-akt - metabolism | Index Medicus
BREAST-CANCER | GROWTH-FACTOR RECEPTOR | PHOSPHORYLATED PEPTIDES | NOVO PYRIMIDINE SYNTHESIS | PROTEIN-KINASE-B | PHOSPHATIDYLINOSITOL 3-KINASE | CELL-MIGRATION | MULTIDISCIPLINARY SCIENCES | ATP-CITRATE LYASE | HIGHLY SELECTIVE ENRICHMENT | TITANIUM-DIOXIDE | RNA, Small Interfering - genetics | Immunoprecipitation | Mutagenesis, Site-Directed | Humans | Immunoblotting | DNA Primers - genetics | Signal Transduction - genetics | Cortactin - metabolism | Phosphoproteins - genetics | Mutation - genetics | Reverse Transcriptase Polymerase Chain Reaction | Gene Knock-In Techniques | Phosphatidylinositol 3-Kinases - genetics | Tandem Mass Spectrometry | Class I Phosphatidylinositol 3-Kinases | Fluorescent Antibody Technique | Proteomics | Chromatography, Liquid | Signal Transduction - physiology | Proto-Oncogene Proteins c-akt - metabolism | Index Medicus
Journal Article
ELIFE, ISSN 2050-084X, 10/2019, Volume 8
Mutations that activate LRRK2 protein kinase cause Parkinson's disease. LRRK2 phosphorylates a subset of Rab GTPases within their Switch-II motif controlling...
PENETRANCE | COMPUTATIONAL PLATFORM | ACID | MECHANISM | PHOSPHORYLATION | KINASE | BIOLOGY | PHENOTYPE | MUTATIONS | IDENTIFICATION | PARKINSONS-DISEASE
PENETRANCE | COMPUTATIONAL PLATFORM | ACID | MECHANISM | PHOSPHORYLATION | KINASE | BIOLOGY | PHENOTYPE | MUTATIONS | IDENTIFICATION | PARKINSONS-DISEASE
Journal Article
Journal of Proteome Research, ISSN 1535-3893, 09/2015, Volume 14, Issue 9, pp. 3882 - 3891
The human oncogene PIK3CA is frequently mutated in human cancers. Two hotspot mutations in PIK3CA, E545K and H1047R, have been shown to regulate widespread...
GROWTH-FACTOR RECEPTOR | KINASE-ACTIVITY | CELLS | GENE | PHOSPHORYLATION | COLORECTAL-CANCER | BIOCHEMICAL RESEARCH METHODS | HIGH-FREQUENCY | AUTOPHOSPHORYLATION | PROTEIN-KINASES | C-SRC | Phosphorylation | Proteome - genetics | Humans | Phosphatidylinositol 3-Kinases - chemistry | Phosphatidylinositol 3-Kinases - metabolism | Signal Transduction - genetics | Phosphoproteins - genetics | Proteome - analysis | Phosphoproteins - metabolism | Proteome - chemistry | Phosphoproteins - chemistry | Proto-Oncogene Proteins c-akt - genetics | Phosphatidylinositol 3-Kinases - genetics | Tyrosine - metabolism | Class I Phosphatidylinositol 3-Kinases | Phosphoproteins - analysis | Proteomics | Cell Line, Tumor | Proto-Oncogene Proteins c-akt - metabolism | Proteome - metabolism
GROWTH-FACTOR RECEPTOR | KINASE-ACTIVITY | CELLS | GENE | PHOSPHORYLATION | COLORECTAL-CANCER | BIOCHEMICAL RESEARCH METHODS | HIGH-FREQUENCY | AUTOPHOSPHORYLATION | PROTEIN-KINASES | C-SRC | Phosphorylation | Proteome - genetics | Humans | Phosphatidylinositol 3-Kinases - chemistry | Phosphatidylinositol 3-Kinases - metabolism | Signal Transduction - genetics | Phosphoproteins - genetics | Proteome - analysis | Phosphoproteins - metabolism | Proteome - chemistry | Phosphoproteins - chemistry | Proto-Oncogene Proteins c-akt - genetics | Phosphatidylinositol 3-Kinases - genetics | Tyrosine - metabolism | Class I Phosphatidylinositol 3-Kinases | Phosphoproteins - analysis | Proteomics | Cell Line, Tumor | Proto-Oncogene Proteins c-akt - metabolism | Proteome - metabolism
Journal Article
8.
Identification of long-lived synaptic proteins by proteomic analysis of synaptosome protein turnover
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 04/2018, Volume 115, Issue 16, p. E3827
Memory formation is believed to result from changes in synapse strength and structure. While memories may persist for the lifetime of an organism, the proteins...
Proteins | Enrichment | Neurons | Memory | Proteomics | Lipids | Mice | Pharmacology | Mass spectrometry | Synapses | Protein turnover | Structure-function relationships
Proteins | Enrichment | Neurons | Memory | Proteomics | Lipids | Mice | Pharmacology | Mass spectrometry | Synapses | Protein turnover | Structure-function relationships
Journal Article
PLoS Pathogens, ISSN 1553-7366, 12/2015, Volume 11, Issue 12
 Epstein-Barr virus (EBV) is etiologically linked to infectious mononucleosis and several human cancers. EBV encodes a conserved protein kinase BGLF4 that...
Proteins | Medical research | Scholarships & fellowships | Phosphorylation | DNA damage | Cell cycle | Proteomics | Ataxia | Kinases | Deoxyribonucleic acid--DNA
Proteins | Medical research | Scholarships & fellowships | Phosphorylation | DNA damage | Cell cycle | Proteomics | Ataxia | Kinases | Deoxyribonucleic acid--DNA
Journal Article
Genome Research, ISSN 1088-9051, 01/2011, Volume 21, Issue 11, pp. 1872 - 1881
Anopheles gambiae is a major mosquito vector responsible for malaria transmission, whose genome sequence was reported in 2002. Genome annotation is a...
Journal Article
Clinical Proteomics, ISSN 1542-6416, 2014, Volume 11, Issue 1, pp. 1 - 1
Background: Rheumatoid arthritis and osteoarthritis are two common musculoskeletal disorders that affect the joints. Despite high prevalence rates, etiological...
Extracellular matrix | Arthritis | Joint inflammation | Cartilage degradation | AUTOANTIBODIES | QUANTITATIVE PROTEOMICS | ALPHA-ENOLASE | BIOCHEMICAL RESEARCH METHODS | GELATINASE-ASSOCIATED LIPOCALIN | IDENTIFICATION | FIBROBLAST ACTIVATION PROTEIN | BIOMARKERS | BIOLOGICAL MARKERS | MOLECULAR-CLONING | GLUCOSE-6-PHOSPHATE ISOMERASE | Rheumatoid factor | Fibrin | Cysteine | Scientific equipment and supplies industry | Analysis | Fibrinogen | Osteoarthritis | Protein kinases | Medical research | Biotechnology | Disease | Architects | Pathogenesis | Rheumatology | Proteins | Medicine | Scholarships & fellowships | Proteomics | Biomarkers | Rheumatism | Labeling | Bioinformatics
Extracellular matrix | Arthritis | Joint inflammation | Cartilage degradation | AUTOANTIBODIES | QUANTITATIVE PROTEOMICS | ALPHA-ENOLASE | BIOCHEMICAL RESEARCH METHODS | GELATINASE-ASSOCIATED LIPOCALIN | IDENTIFICATION | FIBROBLAST ACTIVATION PROTEIN | BIOMARKERS | BIOLOGICAL MARKERS | MOLECULAR-CLONING | GLUCOSE-6-PHOSPHATE ISOMERASE | Rheumatoid factor | Fibrin | Cysteine | Scientific equipment and supplies industry | Analysis | Fibrinogen | Osteoarthritis | Protein kinases | Medical research | Biotechnology | Disease | Architects | Pathogenesis | Rheumatology | Proteins | Medicine | Scholarships & fellowships | Proteomics | Biomarkers | Rheumatism | Labeling | Bioinformatics
Journal Article
PROTEOMICS, ISSN 1615-9853, 03/2017, Volume 17, Issue 6, p. 1770040
Journal Article
PLoS Genetics, ISSN 1553-7390, 11/2016, Volume 12, Issue 11, p. e1006443
The most common cause of the neurodegenerative diseases amyotrophic lateral sclerosis and frontotemporal dementia is a hexanucleotide repeat expansion in...
DIPEPTIDE-REPEAT PROTEINS | FRONTOTEMPORAL DEMENTIA | EXPANSION | GENETICS & HEREDITY | ALS | AMYOTROPHIC-LATERAL-SCLEROSIS | HEXANUCLEOTIDE REPEAT | RNA FOCI | LYSOSOMAL BIOGENESIS | TRANSGENIC MICE | MOTOR DEFICITS | Frontotemporal Dementia - genetics | Ribosomal Protein S6 Kinases, 90-kDa - biosynthesis | Guanine Nucleotide Exchange Factors - genetics | DNA Repeat Expansion - genetics | Amyotrophic Lateral Sclerosis - genetics | Humans | Gene Expression Regulation | Signal Transduction - genetics | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - biosynthesis | Frontotemporal Dementia - metabolism | Carrier Proteins - genetics | Amyotrophic Lateral Sclerosis - pathology | Phenotype | Animals | TOR Serine-Threonine Kinases - genetics | Alleles | Amyotrophic Lateral Sclerosis - metabolism | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - genetics | C9orf72 Protein | Mice | Autophagy - genetics | TOR Serine-Threonine Kinases - biosynthesis | Frontotemporal Dementia - pathology | Physiological aspects | Autophagy (Cytology) | Amyotrophic lateral sclerosis | Rapamycin | Health aspects | Neurodegeneration | Genes | Rodents
DIPEPTIDE-REPEAT PROTEINS | FRONTOTEMPORAL DEMENTIA | EXPANSION | GENETICS & HEREDITY | ALS | AMYOTROPHIC-LATERAL-SCLEROSIS | HEXANUCLEOTIDE REPEAT | RNA FOCI | LYSOSOMAL BIOGENESIS | TRANSGENIC MICE | MOTOR DEFICITS | Frontotemporal Dementia - genetics | Ribosomal Protein S6 Kinases, 90-kDa - biosynthesis | Guanine Nucleotide Exchange Factors - genetics | DNA Repeat Expansion - genetics | Amyotrophic Lateral Sclerosis - genetics | Humans | Gene Expression Regulation | Signal Transduction - genetics | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - biosynthesis | Frontotemporal Dementia - metabolism | Carrier Proteins - genetics | Amyotrophic Lateral Sclerosis - pathology | Phenotype | Animals | TOR Serine-Threonine Kinases - genetics | Alleles | Amyotrophic Lateral Sclerosis - metabolism | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - genetics | C9orf72 Protein | Mice | Autophagy - genetics | TOR Serine-Threonine Kinases - biosynthesis | Frontotemporal Dementia - pathology | Physiological aspects | Autophagy (Cytology) | Amyotrophic lateral sclerosis | Rapamycin | Health aspects | Neurodegeneration | Genes | Rodents
Journal Article
Nature, ISSN 0028-0836, 2014, Volume 509, Issue 7502, pp. 575 - 581
The availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with...
PROJECT | DATABASE | TANDEM | MULTIDISCIPLINARY SCIENCES | KNOWLEDGE | IDENTIFICATION | PROTEINS | Protein Biosynthesis | Proteome - genetics | Humans | Fetus - metabolism | Gene Expression Profiling | Proteome - chemistry | RNA, Untranslated - genetics | Pseudogenes - genetics | Fourier Analysis | Protein Isoforms - metabolism | Databases, Protein | Mass Spectrometry | Adult | Reproducibility of Results | Molecular Sequence Annotation | Cells, Cultured | Protein Isoforms - analysis | Hematopoietic Stem Cells - metabolism | Proteome - analysis | Open Reading Frames - genetics | Organ Specificity | Genome, Human - genetics | Protein Sorting Signals | Protein Transport | Hematopoietic Stem Cells - cytology | Proteomics | Internet | Proteome - metabolism | Untranslated Regions - genetics | Protein Isoforms - genetics | Physiological aspects | Usage | Genomes | Liquid chromatography | B cells | Research | Proteins | Peptides | Genomics | Protein expression | Gene expression | Mass spectrometry | Chromatography | Methods
PROJECT | DATABASE | TANDEM | MULTIDISCIPLINARY SCIENCES | KNOWLEDGE | IDENTIFICATION | PROTEINS | Protein Biosynthesis | Proteome - genetics | Humans | Fetus - metabolism | Gene Expression Profiling | Proteome - chemistry | RNA, Untranslated - genetics | Pseudogenes - genetics | Fourier Analysis | Protein Isoforms - metabolism | Databases, Protein | Mass Spectrometry | Adult | Reproducibility of Results | Molecular Sequence Annotation | Cells, Cultured | Protein Isoforms - analysis | Hematopoietic Stem Cells - metabolism | Proteome - analysis | Open Reading Frames - genetics | Organ Specificity | Genome, Human - genetics | Protein Sorting Signals | Protein Transport | Hematopoietic Stem Cells - cytology | Proteomics | Internet | Proteome - metabolism | Untranslated Regions - genetics | Protein Isoforms - genetics | Physiological aspects | Usage | Genomes | Liquid chromatography | B cells | Research | Proteins | Peptides | Genomics | Protein expression | Gene expression | Mass spectrometry | Chromatography | Methods
Journal Article