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1. Full Text Nuclease‐mediated genome editing: At the front‐line of functional genomics technology
by Sakuma, Tetsushi and Woltjen, Knut
Development, Growth & Differentiation, ISSN 0012-1592, 01/2014, Volume 56, Issue 1, pp. 2 - 13
Genome editing with engineered endonucleases is rapidly becoming a staple method in developmental biology studies. Engineered nucleases permit random or... 
clustered regularly interspaced short palindromic repeats | genome editing | transcription activator‐like effector nucleases | zinc‐finger nucleases | Transcription activator-like effector nucleases | Genome editing | Clustered regularly interspaced short palindromic repeats | Zinc-finger nucleases | zinc-finger nucleases | EMBRYO MICROINJECTION | DEVELOPMENTAL BIOLOGY | transcription activator-like effector nucleases | ONE-STEP GENERATION | CELL BIOLOGY | SINGLE-CHAIN | ZINC-FINGER NUCLEASE | CAENORHABDITIS-ELEGANS | EFFICIENT TARGETED MUTAGENESIS | GENE-EXPRESSION | CHROMOSOMAL DELETIONS | HOMOLOGOUS RECOMBINATION | KNOCKOUT RATS | Genetic Engineering - methods | Animals | Humans | CRISPR-Cas Systems | Developmental Biology - methods | Endodeoxyribonucleases - metabolism | Genome - genetics | Genomics - methods | Genetic research | Genomics | Genomes | DNA repair | DNA damage | Deoxyribonucleic acid--DNA
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2. Full Text Current overview of TALEN construction systems
by Sakuma, Tetsushi and Yamamoto, Takashi
Methods in Molecular Biology, ISSN 1064-3745, 2017, Volume 1630, pp. 25 - 36
Transcription activator-like effector (TALE) nuclease (TALEN) is the second-generation genome editing tool consisting of TALE protein containing customizable... 
Pre-assembled library | Ligation-independent cloning | TALEN | Golden Gate assembly | Serial ligation | Bacteria - growth & development | Animals | Humans | Reagent Kits, Diagnostic | Transcription Activator-Like Effector Nucleases - chemistry | Transcription Activator-Like Effector Nucleases - metabolism | Protein Engineering | Transcription Activator-Like Effector Nucleases - genetics | Bacteria - genetics | Gene Editing
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3. Full Text Engineering customized TALENs using the platinum gate TALEN kit
by Sakuma, Tetsushi and Yamamoto, Takashi
Methods in Molecular Biology, ISSN 1064-3745, 2016, Volume 1338, pp. 61 - 70
Among various strategies for constructing customized transcription activator-like effector nucleases (TALENs), the Golden Gate assembly is the most widely used... 
Platinum Gate TALEN Kit | Platinum TALEN | Modular assembly | Golden Gate assembly | 4-Module assembly | Gene Targeting - methods | Endonucleases - genetics | Protein Engineering - methods | Cloning, Molecular - methods | Endonucleases - biosynthesis | Plasmids - genetics | Trans-Activators - genetics | DNA-Binding Proteins - genetics | Genetic Vectors | Trans-Activators - chemistry | DNA-Binding Proteins - chemistry
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4. Current advances and future prospects of genome editing technology in the field of biomedicine.
by Sakuma, Tetsushi
Clinical calcium, ISSN 0917-5857, 2017, Volume 27, Issue 12, p. 1788
Genome editing technology can alter the genomic sequence at will, contributing the creation of cellular and animal models of human diseases including... 
Animals | Genetic Testing | Neoplasms - genetics | Epigenesis, Genetic | Humans | Clustered Regularly Interspaced Short Palindromic Repeats | Genome, Human | Gene Editing
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5. Full Text Front-line of genome editing technology for animal cell engineering
by Sakuma, Tetsushi
BMC Proceedings, ISSN 1753-6561, 12/2015, Volume 9, Issue S9
  Genome editing technology heralds a new era for animal cell engineering. Programmable site-specific nucleases, such as transcription activator-like effector... 
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6. Full Text Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system
by Sakuma, Tetsushi and Nishikawa, Ayami and Kume, Satoshi and Chayama, Kazuaki and Yamamoto, Takashi
Scientific Reports, ISSN 2045-2322, 06/2014, Volume 4, Issue 1, pp. 5400 - 5400
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted... 
MUTAGENESIS | SPECIFICITY | NICKING | TALEN | MULTIDISCIPLINARY SCIENCES | PLASMID | GENES | GENERATION | MICE | CAS9 | NUCLEASES | Genetic Engineering - methods | Clustered Regularly Interspaced Short Palindromic Repeats - genetics | Transfection - methods | Humans | Genetic Vectors - genetics | Genome, Human - genetics | Targeted mutagenesis | Gene silencing | CRISPR | Gene targeting | Mutagenesis | Transcription activation | Genomes | Nuclease | Ribonucleic acid--RNA | Expression vectors | Index Medicus
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7. Full Text MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems
by Sakuma, Tetsushi and Sakuma, Tetsushi and Nakade, Shota and Nakade, Shota and Sakane, Yuto and Sakane, Yuto and Suzuki, Ken-Ichi T and Suzuki, Ken-Ichi T and Yamamoto, Takashi and Yamamoto, Takashi
Nature protocols, ISSN 1754-2189, 01/2016, Volume 11, Issue 1, pp. 118 - 133
Programmable nucleases enable engineering of the genome by utilizing endogenous DNA double-strand break (DSB) repair pathways. Although homologous... 
CELLS | INTEGRATION | DNA | BIOCHEMICAL RESEARCH METHODS | GENERATION | MUTATIONS | CAS9 | TARGETED MUTAGENESIS | CLEAVAGE | GENOME | DROSOPHILA | Cell Line | Animals | DNA Restriction Enzymes - metabolism | Chromosomes - genetics | DNA End-Joining Repair - genetics | Humans | CRISPR-Cas Systems - genetics | Gene Knock-In Techniques - methods | Sequence Homology, Nucleic Acid | Genetic engineering | Genetic vectors | Properties | Methods | Genetic screening | Innovations
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8. Full Text Simple knockout by electroporation of engineered endonucleases into intact rat embryos
by Kaneko, Takehito and Sakuma, Tetsushi and Yamamoto, Takashi and Mashimo, Tomoji
Scientific Reports, ISSN 2045-2322, 2014, Volume 4, Issue 1, p. 6382
Engineered endonucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly... 
STEM-CELLS | GENE | MODELS | MULTIDISCIPLINARY SCIENCES | MOUSE | ZONA PELLUCIDA | GENERATION | BLASTOCYSTS | ZINC-FINGER NUCLEASES | MICROINJECTION | EGGS | Genetic Engineering - methods | Gene Targeting - methods | Zinc Fingers | Rats, Inbred F344 | Cells, Cultured | Rats | Male | Electroporation - methods | Embryo, Mammalian - metabolism | Endodeoxyribonucleases - genetics | Animals | Embryo Transfer | Embryo, Mammalian - cytology | CRISPR-Cas Systems | Endodeoxyribonucleases - metabolism | Female | Clustered Regularly Interspaced Short Palindromic Repeats - genetics | CRISPR | Microinjection | Transcription | Electroporation | Genomes | Endonuclease | Embryos
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9. Full Text Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice
by Aida, Tomomi and Chiyo, Keiho and Usami, Takako and Ishikubo, Harumi and Imahashi, Risa and Wada, Yusaku and Tanaka, Kenji F and Sakuma, Tetsushi and Yamamoto, Takashi and Tanaka, Kohichi
Genome Biology, ISSN 1474-7596, 04/2015, Volume 16, Issue 1, p. 87
Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we... 
DNA CLEAVAGE | CELL CLONES | RNA | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | OFF-TARGET CLEAVAGE | MOUSE | GENETICS & HEREDITY | MEDIATED DELIVERY | CRISPR-CAS9 | CAS NUCLEASES | GENOME | ONE-STEP GENERATION | Recombinant Proteins - metabolism | Cell Line | Gene Targeting | Humans | RNA, Messenger - genetics | Male | Recombinant Proteins - genetics | Genetic Loci | RNA, Messenger - metabolism | CRISPR-Cas Systems - genetics | Sequence Analysis, DNA | Gene Knock-In Techniques | Mice, Knockout | Animals | Cloning, Molecular | HEK293 Cells | Female | Mice | Genes, Reporter | Proteins | CRISPR | Cytomegalovirus | Cloning | Genes | Rodents | Stem cells | mRNA | Genetic engineering | Genomes | RNA polymerase | Deoxyribonucleic acid--DNA | Method
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10. Full Text Acceleration of cancer science with genome editing and related technologies
by Sakuma, Tetsushi and Yamamoto, Takashi
Cancer Science, ISSN 1347-9032, 12/2018, Volume 109, Issue 12, pp. 3679 - 3685
Genome editing includes various edits of the genome, such as short insertions and deletions, substitutions, and chromosomal rearrangements including... 
cancer science | transcriptome editing | CRISPR | genome editing | Cas9 | base editing | CRISPR-Cas9 | LONG NONCODING RNAS | HUMAN-CELLS | NUCLEIC-ACID DETECTION | IDENTIFICATION | BASE | ONCOLOGY | DNA | GENETIC SCREENS | REGIONS | GROWTH | WIDE CRISPR SCREEN | Epigenomics | Sequence Analysis, RNA | Neoplasms - genetics | Humans | Biomedical Research | Gene Editing - methods | Gene Expression Profiling | Epigenetic inheritance | Genetic aspects | Genomics | Cancer | RNA editing | Nucleotide sequence | Laboratories | DNA damage | Genes | Science | Genomes | Double-strand break repair | Chromosome rearrangements | Gene expression | Proteins | Chromosome translocations | Technological change | Proteomics | Epigenetics | Genetic engineering | Labeling | Deoxyribonucleic acid--DNA | Review | CRISPR‐Cas9
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11. Full Text Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9
by Nakade, Shota and Tsubota, Takuya and Sakane, Yuto and Kume, Satoshi and Sakamoto, Naoaki and Obara, Masanobu and Daimon, Takaaki and Sezutsu, Hideki and Yamamoto, Takashi and Sakuma, Tetsushi and Suzuki, Ken-Ichi T
Nature Communications, ISSN 2041-1723, 2014, Volume 5, Issue 1, p. 5560
Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting... 
SYSTEM | REPAIR | MULTIDISCIPLINARY SCIENCES | GENES | TARGETED INTEGRATION | CLEAVAGE | ZINC-FINGER NUCLEASES | Xenopus | Humans | Molecular Sequence Data | Homologous Recombination | DNA - metabolism | Deoxyribonucleases | Bombyx | Animals | Genetic Engineering | Plasmids | Base Sequence | CRISPR-Cas Systems | Genetic Vectors | Gene Knock-In Techniques - methods | Saccharomyces cerevisiae
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12. Cancer induction and suppression with transcriptional control and epigenome editing technologies
by Nakade, Shota and Yamamoto, Takashi and Sakuma, Tetsushi
Journal of Human Genetics, ISSN 1434-5161, 02/2018, Volume 63, Issue 2, pp. 187 - 194
Cancer epigenetics is one of the most important research subjects in dissecting cancer mechanisms and therapeutic targets because the emergence and malignant... 
METHYLATION | ACTIVATION | HUMAN GENOME | ZINC-FINGER PROTEINS | IN-VIVO | ENDOGENOUS GENES | GENETICS & HEREDITY | FUSION PROTEIN | CROSS-LINKING | TARGETED DNA DEMETHYLATION | EXPRESSION | Neoplasms - metabolism | Gene Expression Regulation - genetics | Humans | Cell Transformation, Neoplastic - metabolism | Neoplasms - therapy | Animals | Epigenomics - methods | Neoplasms - genetics | Cell Transformation, Neoplastic - genetics | Epigenesis, Genetic - genetics | Gene Editing - methods | Transcription, Genetic - genetics | Cell Transformation, Neoplastic - pathology | Neoplasms - pathology
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13. Full Text Magic wands of CRISPR—lots of choices for gene knock-in
by Sakuma, Tetsushi and Yamamoto, Takashi
Cell Biology and Toxicology, ISSN 0742-2091, 12/2017, Volume 33, Issue 6, pp. 501 - 505
To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s10565-017-9409-6 
Life Sciences | Biochemistry, general | Pharmacology/Toxicology | Cell Biology | INSERTION | REPAIR | ZEBRAFISH | TALEN | TARGETED INTEGRATION | GENERATION | TOXICOLOGY | MAMMALIAN-CELLS | EFFICIENCY | GENOME | OLIGONUCLEOTIDES | CELL BIOLOGY | Genetic research | CRISPR | Genomics | Index Medicus
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14. Full Text Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish
by Hisano, Yu and Sakuma, Tetsushi and Nakade, Shota and Ohga, Rie and Ota, Satoshi and Okamoto, Hitoshi and Yamamoto, Takashi and Kawahara, Atsuo
Scientific Reports, ISSN 2045-2322, 2015, Volume 5, Issue 1, p. 8841
The CRISPR/Cas9 system provides a powerful tool for genome editing in various model organisms, including zebrafish. The establishment of targeted... 
KNOCK-IN | RECOMBINATION | REPAIR | TALENS | MULTIDISCIPLINARY SCIENCES | GENES | TARGETED INTEGRATION | GENERATION | ZINC-FINGER NUCLEASES | GENOME MODIFICATIONS | CAS9 NUCLEASE | Amino Acid Sequence | Gene Targeting - methods | Molecular Sequence Data | Homologous Recombination | Genetic Loci | Genetic Vectors - genetics | Zebrafish - genetics | Gene Knock-In Techniques | Animals | Base Sequence | CRISPR-Cas Systems | Genome | Genes, Reporter | CRISPR | Integration | Nucleotide sequence | Zebrafish | Homology | Genomes | DNA repair | Deoxyribonucleic acid--DNA
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15. Full Text Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells
by Ochiai, Hiroshi and Sugawara, Takeshi and Sakuma, Tetsushi and Yamamoto, Takashi
Scientific Reports, ISSN 2045-2322, 2014, Volume 4, Issue 1, pp. 7125 - 7125
Mouse embryonic stem cells (mESCs) are self-renewing and capable of differentiating into any of the three germ layers. An interesting feature of mESCs is the... 
HETEROGENEITY | GENE-EXPRESSION | PLURIPOTENCY | DIFFERENTIATION | GROUND-STATE | MULTIDISCIPLINARY SCIENCES | Cell Line | Embryonic Stem Cells - metabolism | Promoter Regions, Genetic | Embryonic Stem Cells - cytology | Nanog Homeobox Protein | Signal Transduction | Homeodomain Proteins - metabolism | Mice, Inbred C57BL | Gene Expression Regulation | Molecular Sequence Data | In Situ Hybridization, Fluorescence | Mice, Transgenic | Homeodomain Proteins - genetics | Genetic Variation | Animals | Stochastic Processes | Base Sequence | Transcription, Genetic | Cell Differentiation | Mice | Primary Cell Culture | Cell fate | Noise | Transcription activation | Stem cells | Embryo cells | Fluorescence in situ hybridization | Stem cell transplantation | Stochasticity | Gene expression | Embryos | Pluripotency | Index Medicus
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