2009, Springer protocols, ISBN 1597455423, Volume 536, xiii, 588
Over the past 30 years, the development of the Western blot has revolutionized the fields of biomedical research & medical diagnostics. In this book, experts...
Western immunoblotting | Life Sciences | Protein Science | Proteomics
Western immunoblotting | Life Sciences | Protein Science | Proteomics
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Development of Autoantibodies before the Clinical Onset of Systemic Lupus Erythematosus
The New England Journal of Medicine, ISSN 0028-4793, 10/2003, Volume 349, Issue 16, pp. 1526 - 1533
In 130 military personnel, systemic lupus erythematosus (SLE) developed after blood samples had been stored in the U.S. Department of Defense Serum Repository....
MOTHERS | MEDICINE, GENERAL & INTERNAL | WILCOXON TEST | ANTI-DNA ANTIBODIES | ANTINUCLEAR | AUTOIMMUNITY | SJOGRENS-SYNDROME | CONGENITAL HEART-BLOCK | DSDNA | CHILDREN | Prospective Studies | DNA - immunology | Military Personnel | snRNP Core Proteins | Autoantibodies - blood | Humans | Autoantigens | Lupus Erythematosus, Systemic - diagnosis | Autoantibodies - physiology | Time Factors | Antibodies, Antinuclear - blood | Ribonucleoproteins, Small Nuclear - immunology | Lupus Erythematosus, Systemic - immunology | Ribonucleoproteins - immunology | Development and progression | Autoantibodies | Systemic lupus erythematosus | Disease management | Disease | Drug therapy | Immune system
MOTHERS | MEDICINE, GENERAL & INTERNAL | WILCOXON TEST | ANTI-DNA ANTIBODIES | ANTINUCLEAR | AUTOIMMUNITY | SJOGRENS-SYNDROME | CONGENITAL HEART-BLOCK | DSDNA | CHILDREN | Prospective Studies | DNA - immunology | Military Personnel | snRNP Core Proteins | Autoantibodies - blood | Humans | Autoantigens | Lupus Erythematosus, Systemic - diagnosis | Autoantibodies - physiology | Time Factors | Antibodies, Antinuclear - blood | Ribonucleoproteins, Small Nuclear - immunology | Lupus Erythematosus, Systemic - immunology | Ribonucleoproteins - immunology | Development and progression | Autoantibodies | Systemic lupus erythematosus | Disease management | Disease | Drug therapy | Immune system
Journal Article
Nature Genetics, ISSN 1061-4036, 11/2013, Volume 45, Issue 11, pp. 1284 - 1294
Sjogren's syndrome is a common autoimmune disease (affecting similar to 0.7% of European Americans) that typically presents as keratoconjunctivitis sicca and...
RHEUMATOID-ARTHRITIS | B-CELLS | SYSTEMIC-LUPUS-ERYTHEMATOSUS | FUNCTIONAL POLYMORPHISM | STRONG RISK-FACTOR | GENETIC RISK | GENETICS & HEREDITY | SUSCEPTIBILITY LOCI | IRF5 | CLASS-II | GENOME-WIDE ASSOCIATION | Genetic Variation | Histocompatibility Antigens Class II - immunology | Genetic Association Studies | Humans | Genetic Loci - genetics | Immunity, Innate - genetics | Sjogren's Syndrome - immunology | Sjogren's Syndrome - genetics | Adaptive Immunity - genetics | Quantitative trait loci | Sjogren's syndrome | Immune response | Genetic variation | Genetic aspects | Properties | Identification and classification | Studies | Medical research | Genomes | Logistics | Adaptive Immunity | Life Sciences | Human health and pathology | Histocompatibility Antigens Class II | Rhumatology and musculoskeletal system | Genetic Loci | Immunity, Innate | Sjogren's Syndrome | Teknik och teknologier | TEKNIKVETENSKAP | Engineering and Technology | TECHNOLOGY
RHEUMATOID-ARTHRITIS | B-CELLS | SYSTEMIC-LUPUS-ERYTHEMATOSUS | FUNCTIONAL POLYMORPHISM | STRONG RISK-FACTOR | GENETIC RISK | GENETICS & HEREDITY | SUSCEPTIBILITY LOCI | IRF5 | CLASS-II | GENOME-WIDE ASSOCIATION | Genetic Variation | Histocompatibility Antigens Class II - immunology | Genetic Association Studies | Humans | Genetic Loci - genetics | Immunity, Innate - genetics | Sjogren's Syndrome - immunology | Sjogren's Syndrome - genetics | Adaptive Immunity - genetics | Quantitative trait loci | Sjogren's syndrome | Immune response | Genetic variation | Genetic aspects | Properties | Identification and classification | Studies | Medical research | Genomes | Logistics | Adaptive Immunity | Life Sciences | Human health and pathology | Histocompatibility Antigens Class II | Rhumatology and musculoskeletal system | Genetic Loci | Immunity, Innate | Sjogren's Syndrome | Teknik och teknologier | TEKNIKVETENSKAP | Engineering and Technology | TECHNOLOGY
Journal Article
The Lancet, ISSN 0140-6736, 05/2004, Volume 363, Issue 9420, pp. 1544 - 1546
Many human diseases are the result of autoimmune attack, presumably related to a loss of tolerance to self. Autoimmune disease can be divided into either...
RHEUMATOID-ARTHRITIS | STEROID 21-HYDROXYLASE AUTOANTIBODIES | ADRENOCORTICAL AUTOANTIBODIES | ADULT PATIENTS | MEDICINE, GENERAL & INTERNAL | AUTOIMMUNE-DISEASES | ADRENAL AUTOANTIBODIES | LUPUS-ERYTHEMATOSUS | FOLLOW-UP | CLINICAL ADDISONS-DISEASE | HEART-BLOCK | Pregnancy | Autoantibodies - blood | Humans | Female | Autoimmune Diseases - diagnosis | Biomarkers - blood | Pregnancy Complications - diagnosis | Arthritis, Rheumatoid - immunology | Arthritis, Rheumatoid - diagnosis | Pregnancy Complications - immunology | Care and treatment | Autoantibodies | Research | Autoimmune diseases | Health aspects | Rheumatoid arthritis | Health | Immune system | Pathogenesis | Clinical trials | Lymphocytes T | Diabetes mellitus (insulin dependent) | Arthritis | Proteins | Immunology | Illnesses | Systemic lupus erythematosus | Arginine | Lymphocytes | Post-translation | Population | Thyroid | Lupus | Medical research | Immunoglobulins | Diabetes mellitus | Thyroid diseases | Citrulline | Patients | Immunological tolerance | Blood donors | Diseases | Studies | Babies | Rheumatoid factor | Chronic conditions | Womens health | Predictions | Diabetes | Health risk assessment
RHEUMATOID-ARTHRITIS | STEROID 21-HYDROXYLASE AUTOANTIBODIES | ADRENOCORTICAL AUTOANTIBODIES | ADULT PATIENTS | MEDICINE, GENERAL & INTERNAL | AUTOIMMUNE-DISEASES | ADRENAL AUTOANTIBODIES | LUPUS-ERYTHEMATOSUS | FOLLOW-UP | CLINICAL ADDISONS-DISEASE | HEART-BLOCK | Pregnancy | Autoantibodies - blood | Humans | Female | Autoimmune Diseases - diagnosis | Biomarkers - blood | Pregnancy Complications - diagnosis | Arthritis, Rheumatoid - immunology | Arthritis, Rheumatoid - diagnosis | Pregnancy Complications - immunology | Care and treatment | Autoantibodies | Research | Autoimmune diseases | Health aspects | Rheumatoid arthritis | Health | Immune system | Pathogenesis | Clinical trials | Lymphocytes T | Diabetes mellitus (insulin dependent) | Arthritis | Proteins | Immunology | Illnesses | Systemic lupus erythematosus | Arginine | Lymphocytes | Post-translation | Population | Thyroid | Lupus | Medical research | Immunoglobulins | Diabetes mellitus | Thyroid diseases | Citrulline | Patients | Immunological tolerance | Blood donors | Diseases | Studies | Babies | Rheumatoid factor | Chronic conditions | Womens health | Predictions | Diabetes | Health risk assessment
Journal Article
Journal of Rheumatology, ISSN 0315-162X, 11/2018, Volume 45, Issue 11, pp. 1495 - 1496
PATHOGENESIS | BIOMARKERS | PREDICTORS | LYMPHOMA | RISK | PREVALENCE | RHEUMATOLOGY | MANIFESTATIONS
Journal Article
Nutrients, ISSN 2072-6643, 09/2017, Volume 9, Issue 9, p. 949
Osteoarthritis (OA), the most common form of arthritis, is a significant public health burden in U.S. adults. Among its many risk factors, obesity is a key...
Strawberries | Inflammation | Knee osteoarthritis | Pain | ICOAP | COLLAGEN-INDUCED ARTHRITIS | CONTROLLED-TRIAL | pain | METABOLIC SYNDROME | MANAGEMENT | SYNOVIAL-FLUID | MATRIX METALLOPROTEINASES | NUTRITION & DIETETICS | PLASMA | inflammation | knee osteoarthritis | DOUBLE-BLIND | strawberries | INDEX | Arthralgia - etiology | Obesity - immunology | Humans | Middle Aged | Male | Osteoarthritis, Knee - diet therapy | Obesity - blood | Knee Joint - immunology | Female | Osteoarthritis, Knee - immunology | Fruit and Vegetable Juices | Arthralgia - prevention & control | Body Mass Index | Severity of Illness Index | Fragaria | Double-Blind Method | Fruit | Freeze Drying | Inflammation Mediators - blood | Knee Joint - diagnostic imaging | Obesity - physiopathology | Biomarkers - blood | Functional Food | Osteoarthritis, Knee - diagnostic imaging | Cross-Over Studies | Pilot Projects | Quality of Life | Osteoarthritis, Knee - physiopathology | Pain Measurement | Knee | C-reactive protein | Interleukin | Clinical trials | Arthritis | Matrix metalloproteinase | Risk factors | Fruits | Visual perception | Cartilage | Body mass index | Randomization | Bioactive compounds | Polyphenols | Biocompatibility | Curcumin | Bioindicators | Metalloproteinase | Supplementation | Indicators | Public health | Obesity | Berries | Dietary supplements | Knee (anatomy) | Risk analysis | IL-1β | Quality of life | Tea | Diet | Body mass | Biomarkers | Adults | Osteoarthritis | Cartilage diseases
Strawberries | Inflammation | Knee osteoarthritis | Pain | ICOAP | COLLAGEN-INDUCED ARTHRITIS | CONTROLLED-TRIAL | pain | METABOLIC SYNDROME | MANAGEMENT | SYNOVIAL-FLUID | MATRIX METALLOPROTEINASES | NUTRITION & DIETETICS | PLASMA | inflammation | knee osteoarthritis | DOUBLE-BLIND | strawberries | INDEX | Arthralgia - etiology | Obesity - immunology | Humans | Middle Aged | Male | Osteoarthritis, Knee - diet therapy | Obesity - blood | Knee Joint - immunology | Female | Osteoarthritis, Knee - immunology | Fruit and Vegetable Juices | Arthralgia - prevention & control | Body Mass Index | Severity of Illness Index | Fragaria | Double-Blind Method | Fruit | Freeze Drying | Inflammation Mediators - blood | Knee Joint - diagnostic imaging | Obesity - physiopathology | Biomarkers - blood | Functional Food | Osteoarthritis, Knee - diagnostic imaging | Cross-Over Studies | Pilot Projects | Quality of Life | Osteoarthritis, Knee - physiopathology | Pain Measurement | Knee | C-reactive protein | Interleukin | Clinical trials | Arthritis | Matrix metalloproteinase | Risk factors | Fruits | Visual perception | Cartilage | Body mass index | Randomization | Bioactive compounds | Polyphenols | Biocompatibility | Curcumin | Bioindicators | Metalloproteinase | Supplementation | Indicators | Public health | Obesity | Berries | Dietary supplements | Knee (anatomy) | Risk analysis | IL-1β | Quality of life | Tea | Diet | Body mass | Biomarkers | Adults | Osteoarthritis | Cartilage diseases
Journal Article
Annals of the Rheumatic Diseases, ISSN 0003-4967, 03/2013, Volume 72, Issue 3, pp. 437 - 444
Objectives The Xq28 region containing IRAK1 and MECP2 has been identified as a risk locus for systemic lupus erythematosus (SLE) in previous genetic...
GENETIC SUSCEPTIBILITY | KAPPA-B ACTIVATION | REPLICATION | VARIANTS | BINDING-PROTEIN | HAPLOTYPE | RHEUMATOLOGY | IDENTIFICATION | ACETYLTRANSFERASE ARD1 | GENOME-WIDE ASSOCIATION | INSIGHTS | Haplotypes | Genetic Predisposition to Disease - genetics | Chromosomes, Human, X - genetics | Interleukin-1 Receptor-Associated Kinases - genetics | Humans | Risk Factors | Molecular Sequence Data | Genotype | Chromosome Mapping | Continental Population Groups - genetics | Methyl-CpG-Binding Protein 2 - genetics | Base Sequence | Lupus Erythematosus, Systemic - genetics | Polymorphism, Single Nucleotide | Real-Time Polymerase Chain Reaction | Chromosome mapping | Usage | Systemic lupus erythematosus | Genetic variation | Genetic aspects | Research | Risk factors | Lupus | Genealogy | Cytokines | Pathogenesis | Mortality | Principal components analysis | Genomes | Gender | Kinases | Gene expression | Studies | Genotype & phenotype | Collaboration | DNA methylation | Epigenetics | Genetic testing | Deoxyribonucleic acid--DNA | Systemic Lupus Erythematosus | MECP2 | Gene Polymorphism | IRAK1 | Xq28
GENETIC SUSCEPTIBILITY | KAPPA-B ACTIVATION | REPLICATION | VARIANTS | BINDING-PROTEIN | HAPLOTYPE | RHEUMATOLOGY | IDENTIFICATION | ACETYLTRANSFERASE ARD1 | GENOME-WIDE ASSOCIATION | INSIGHTS | Haplotypes | Genetic Predisposition to Disease - genetics | Chromosomes, Human, X - genetics | Interleukin-1 Receptor-Associated Kinases - genetics | Humans | Risk Factors | Molecular Sequence Data | Genotype | Chromosome Mapping | Continental Population Groups - genetics | Methyl-CpG-Binding Protein 2 - genetics | Base Sequence | Lupus Erythematosus, Systemic - genetics | Polymorphism, Single Nucleotide | Real-Time Polymerase Chain Reaction | Chromosome mapping | Usage | Systemic lupus erythematosus | Genetic variation | Genetic aspects | Research | Risk factors | Lupus | Genealogy | Cytokines | Pathogenesis | Mortality | Principal components analysis | Genomes | Gender | Kinases | Gene expression | Studies | Genotype & phenotype | Collaboration | DNA methylation | Epigenetics | Genetic testing | Deoxyribonucleic acid--DNA | Systemic Lupus Erythematosus | MECP2 | Gene Polymorphism | IRAK1 | Xq28
Journal Article
The American Journal of Human Genetics, ISSN 0002-9297, 04/2014, Volume 94, Issue 4, pp. 586 - 598
Efforts to identify lupus-associated causal variants in the locus on 8p21 are hampered by highly associated noncausal variants. In this report, we used a...
GENOTYPE DATA | PROTEIN | GENE | GENETICS & HEREDITY | SUSCEPTIBILITY LOCI | SJOGRENS-SYNDROME | CHINESE HAN | B LYMPHOID KINASE | EXPRESSION | ERYTHEMATOSUS | GENOME-WIDE ASSOCIATION | Haplotypes | Promoter Regions, Genetic | Genetic Predisposition to Disease | Humans | Male | Chromosomes, Human, Pair 8 | Lupus Erythematosus, Systemic - genetics | Alleles | Female | Transcription, Genetic | Electrophoretic Mobility Shift Assay | Polymorphism, Single Nucleotide | src-Family Kinases - genetics | Lupus | Allelomorphism | Medical genetics | Genetic research | Genetic aspects | Genetic transcription | Research | Genetic regulation | Identification and classification | Health aspects
GENOTYPE DATA | PROTEIN | GENE | GENETICS & HEREDITY | SUSCEPTIBILITY LOCI | SJOGRENS-SYNDROME | CHINESE HAN | B LYMPHOID KINASE | EXPRESSION | ERYTHEMATOSUS | GENOME-WIDE ASSOCIATION | Haplotypes | Promoter Regions, Genetic | Genetic Predisposition to Disease | Humans | Male | Chromosomes, Human, Pair 8 | Lupus Erythematosus, Systemic - genetics | Alleles | Female | Transcription, Genetic | Electrophoretic Mobility Shift Assay | Polymorphism, Single Nucleotide | src-Family Kinases - genetics | Lupus | Allelomorphism | Medical genetics | Genetic research | Genetic aspects | Genetic transcription | Research | Genetic regulation | Identification and classification | Health aspects
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2019, Volume 1855, pp. 511 - 518
In spite of taking precautions, some common mistakes creep into well-planned gel electrophoresis experiments. This occurs commonly in relation to calculating...
Artifacts | Keratin | Aggregates | Proteases | Asp-Pro bond | Common mistakes in electrophoresis | Cross-linking factor | Ammonium cyanate | Protein Aggregates | Temperature | Cross-Linking Reagents - chemistry | Humans | Electrophoresis, Gel, Two-Dimensional - methods | Polymerization | Proteins - analysis | Urea - analysis | Acrylic Resins - chemistry | Peptide Hydrolases - chemistry | Electrophoresis, Polyacrylamide Gel - methods | Keratins - analysis | Algorithms | Animals | Proteolysis | Gels - chemistry | Buffers | Cyanates - analysis
Artifacts | Keratin | Aggregates | Proteases | Asp-Pro bond | Common mistakes in electrophoresis | Cross-linking factor | Ammonium cyanate | Protein Aggregates | Temperature | Cross-Linking Reagents - chemistry | Humans | Electrophoresis, Gel, Two-Dimensional - methods | Polymerization | Proteins - analysis | Urea - analysis | Acrylic Resins - chemistry | Peptide Hydrolases - chemistry | Electrophoresis, Polyacrylamide Gel - methods | Keratins - analysis | Algorithms | Animals | Proteolysis | Gels - chemistry | Buffers | Cyanates - analysis
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2019, Volume 1855, pp. 93 - 100
Isoelectric focusing (IEF) serves as a very useful procedure for cell protein separation and characterization. We have used this method to study antibody...
Affinity immunoblotting | Ro 60 autoantigen | Systemic lupus erythematosus | Flatbed IEF | Clonotype distribution | Ribonucleoproteins - isolation & purification | Humans | Isoelectric Focusing | Ribonucleoproteins - blood | Lupus Erythematosus, Systemic - metabolism | Immunoblotting | Lupus Erythematosus, Systemic - blood | Collodion - chemistry
Affinity immunoblotting | Ro 60 autoantigen | Systemic lupus erythematosus | Flatbed IEF | Clonotype distribution | Ribonucleoproteins - isolation & purification | Humans | Isoelectric Focusing | Ribonucleoproteins - blood | Lupus Erythematosus, Systemic - metabolism | Immunoblotting | Lupus Erythematosus, Systemic - blood | Collodion - chemistry
Journal Article
The Journal of Clinical Endocrinology & Metabolism, ISSN 0021-972X, 03/2016, Volume 101, Issue 3, pp. L38 - L39
Journal Article
The American Journal of Human Genetics, ISSN 0002-9297, 04/2012, Volume 90, Issue 4, pp. 648 - 660
Systemic lupus erythematosus (SLE) is a chronic heterogeneous autoimmune disorder characterized by the loss of tolerance to self-antigens and dysregulated...
RHEUMATOID-ARTHRITIS | GENETIC SUSCEPTIBILITY | IMMUNE-RESPONSE | RISK LOCI | CROHNS-DISEASE | VARIANTS | PRIMARY BILIARY-CIRRHOSIS | GENETICS & HEREDITY | FOLLOW-UP | FAMILIAL AGGREGATION | GENOME-WIDE ASSOCIATION | European Continental Ancestry Group - genetics | Genetic Predisposition to Disease | Hispanic Americans - genetics | Membrane Proteins - genetics | Humans | Asian Continental Ancestry Group - genetics | Interferon Regulatory Factors - genetics | Male | Chromosome Mapping | Sequence Analysis, DNA | Ikaros Transcription Factor - genetics | Haplotypes - genetics | Lupus Erythematosus, Systemic - ethnology | Lupus Erythematosus, Systemic - genetics | Indians, North American - genetics | Polymorphism, Single Nucleotide - genetics | Female | African Continental Ancestry Group - genetics | Egg Proteins - genetics | Europeans | Lupus erythematosus | Genetic aspects | Research | Genetic transcription | Gene expression | Genetic regulation | Risk factors | Diseases | Lupus | Disease susceptibility | Systemic lupus erythematosus | Racially mixed people | Analysis | Haplotypes | Ikaros protein | Autoantigens | Environmental factors | Immunological tolerance | Zona pellucida | Membrane proteins | Etiology | Interleukin 1 | Replication | Zinc finger proteins | Autoimmune diseases | Gene mapping | Interferon regulatory factor | Medical and Health Sciences | Medicin och hälsovetenskap
RHEUMATOID-ARTHRITIS | GENETIC SUSCEPTIBILITY | IMMUNE-RESPONSE | RISK LOCI | CROHNS-DISEASE | VARIANTS | PRIMARY BILIARY-CIRRHOSIS | GENETICS & HEREDITY | FOLLOW-UP | FAMILIAL AGGREGATION | GENOME-WIDE ASSOCIATION | European Continental Ancestry Group - genetics | Genetic Predisposition to Disease | Hispanic Americans - genetics | Membrane Proteins - genetics | Humans | Asian Continental Ancestry Group - genetics | Interferon Regulatory Factors - genetics | Male | Chromosome Mapping | Sequence Analysis, DNA | Ikaros Transcription Factor - genetics | Haplotypes - genetics | Lupus Erythematosus, Systemic - ethnology | Lupus Erythematosus, Systemic - genetics | Indians, North American - genetics | Polymorphism, Single Nucleotide - genetics | Female | African Continental Ancestry Group - genetics | Egg Proteins - genetics | Europeans | Lupus erythematosus | Genetic aspects | Research | Genetic transcription | Gene expression | Genetic regulation | Risk factors | Diseases | Lupus | Disease susceptibility | Systemic lupus erythematosus | Racially mixed people | Analysis | Haplotypes | Ikaros protein | Autoantigens | Environmental factors | Immunological tolerance | Zona pellucida | Membrane proteins | Etiology | Interleukin 1 | Replication | Zinc finger proteins | Autoimmune diseases | Gene mapping | Interferon regulatory factor | Medical and Health Sciences | Medicin och hälsovetenskap
Journal Article