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1. Full Text AJICAP: Affinity Peptide Mediated Regiodivergent Functionalization of Native Antibodies
by Yamada, Kei and Shikida, Natsuki and Shimbo, Kazutaka and Ito, Yuji and Khedri, Zahra and Matsuda, Yutaka and Mendelsohn, Brian A
Angewandte Chemie International Edition, ISSN 1433-7851, 04/2019, Volume 58, Issue 17, pp. 5592 - 5597
The need for atom‐precise biomolecule modification, and particularly the irreversible formation of covalent bonds to specific amino acids in proteins, has... 
affinity peptides | bioconjugation | AJICAP | antibody–drug conjugates | regiodivergent synthesis | antibody-drug conjugates | CHEMISTRY, MULTIDISCIPLINARY | PROBES | Proteins | Biological products | Thiols | Lysine | Analysis | Immunoglobulin G | Protein binding | Peptides | Xenotransplantation | Antibodies | Cytotoxicity | Amino acids | ErbB-2 protein | Covalent bonds | Organic chemistry | Reagents | Xenografts | Affinity | Biomolecules | Surface plasmon resonance | Functional groups
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2. Highly sensitive quantification for human plasma-targeted metabolomics using an amine derivatization reagent
by Naoko Arashida and Rumi Nishimoto and Masashi Harada and Kazutaka Shimbo and Naoyuki Yamada
Analytica Chimica Acta, ISSN 0003-2670, 02/2017, Volume 954, p. 77
Amino acids and their related metabolites play important roles in various physiological processes and have consequently become biomarkers for diseases.... 
Metabolomics | Amines | Low concentrations | Amino acids | Mass spectroscopy | Liquid chromatography | Chromatography | Blood plasma | Spermine | Metabolites | Linearity | Biomarkers | Physiology | Mass spectrometry | Plasmas (physics)
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3. Full Text Multifunctional and Highly Sensitive Precolumn Reagents for Amino Acids in Liquid Chromatography/Tandem Mass Spectrometry
by Shimbo, Kazutaka and Yahashi, Akihisa and Hirayama, Kazuo and Nakazawa, Masakazu and Miyano, Hiroshi
Analytical Chemistry, ISSN 0003-2700, 07/2009, Volume 81, Issue 13, pp. 5172 - 5179
We have developed novel precolumn derivatization reagent, p-N,N,N-trimethylammonioanilyl N′-hydroxysuccinimidyl carbamate iodide (TAHS), for sensitive analyses... 
SYSTEM | CHEMISTRY, ANALYTICAL | QUANTIFICATION | Amino Acids - blood | Chromatography, High Pressure Liquid - methods | Amino Acids - analysis | Rats | Succinimides - chemical synthesis | Chromatography, High Pressure Liquid - instrumentation | Rats, Sprague-Dawley | Carbamates - chemistry | Succinimides - chemistry | Carbamates - chemical synthesis | Animals | Spectrometry, Mass, Electrospray Ionization - methods | Staining and Labeling | Indicators and Reagents | Tandem Mass Spectrometry - methods | Measurement | Amino acids | Equipment and supplies | Mass spectrometry | Identification and classification | High performance liquid chromatography
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4. Full Text Quantitative profiling of caspase-cleaved substrates reveals different drug-induced and cell-type patterns in apoptosis
by Kazutaka Shimbo and Gerald W. Hsu and Huy Nguyen and Sami Mahrus and Jonathan C. Trinidad and Alma L. Burlingame and James A. Wells
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 7/2012, Volume 109, Issue 31, pp. 12432 - 12437
Proapoptotic drugs are a mainstay of cancer drug treatment. These drugs stress cells and ultimately trigger the activation of caspases, cysteine-class... 
Proteins | T lymphocytes | Cell death | Cell lines | Proteomics | Antineoplastics | Plasma cells | Proteomes | Viability | Apoptosis | Degradomics | Selected reaction monitoring | Mass spectrometry | mass spectrometry | degradomics | proteomics | selected reaction monitoring | EXPRESSION | MULTIDISCIPLINARY SCIENCES | Bortezomib | Drug Screening Assays, Antitumor - methods | Apoptosis - drug effects | Jurkat Cells | Humans | Antineoplastic Agents - chemistry | Neoplasm Proteins - metabolism | Multiple Myeloma - drug therapy | Caspases - metabolism | Cytotoxins - pharmacology | Activating Transcription Factor 4 - metabolism | Cytotoxins - chemistry | Biomarkers, Tumor - metabolism | Antineoplastic Agents - pharmacology | Kinetics | Multiple Myeloma - enzymology | Pyrazines - pharmacology | Proteome - metabolism | Boronic Acids - pharmacology | Care and treatment | Physiological aspects | Research | Health aspects | Methods | Cancer | Biological Sciences
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5. Full Text Unraveling the mechanism of cell death induced by chemical fibrils
by Julien, Olivier and Kampmann, Martin and Bassik, Michael C and Zorn, Julie A and Venditto, Vincent J and Shimbo, Kazutaka and Agard, Nicholas J and Shimada, Kenichi and Rheingold, Arnold L and Stockwell, Brent R and Weissman, Jonathan S and Wells, James A
Nature Chemical Biology, ISSN 1552-4450, 11/2014, Volume 10, Issue 11, pp. 969 - 976
We previously discovered a small-molecule inducer of cell death, named 1541, that noncovalently self-assembles into chemical fibrils ('chemi-fibrils') and... 
PLATFORM | APOPTOSIS | PROTEIN | DISEASES | PROCASPASE-3 | BIOCHEMISTRY & MOLECULAR BIOLOGY | INHIBITORS | MATURATION | GENETIC INTERACTION MAPS | RAB GTPASES | PROPAGATION | Lysosomes - drug effects | Amino Acid Sequence | Lysosomes - chemistry | Humans | Caspase 3 - metabolism | Coumarins - chemistry | Structure-Activity Relationship | Coumarins - pharmacology | Dose-Response Relationship, Drug | Lysosomes - metabolism | Models, Biological | K562 Cells | Benzamides - pharmacology | Cell Proliferation - drug effects | Molecular Structure | Cell Death - drug effects | Tumor Cells, Cultured | Benzamides - chemistry | Proteins | Cytotoxicity | Molecular biology | Apoptosis | Index Medicus
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6. Full Text MP16-04 THE ROLE OF G8 SCREENING TOOL IN THE ASSESSMENT OF SURGICAL OUTCOME OF ELDERLY PATIENTS (= 75 Y.O.) WITH HIGH-RISK PROSTATE CANCER: A PILOT STUDY
by Matsushita, Kazuhito and Sandhu, Jaspreet and Horie, Shigeo and Endo, Fumiyasu and Shimbo, Masaki and Narimoto, Kazutaka and Hattori, Kazunori
The Journal of Urology, ISSN 0022-5347, 04/2018, Volume 199, Issue 4, pp. e198 - e198
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7. Full Text Biaryl axially chiral derivatizing agent for simultaneous separation and sensitive detection of proteinogenic amino acid enantiomers using liquid chromatography–tandem mass spectrometry
by Harada, Masashi and Karakawa, Sachise and Yamada, Naoyuki and Miyano, Hiroshi and Shimbo, Kazutaka
Journal of Chromatography A, ISSN 0021-9673, 05/2019, Volume 1593, pp. 91 - 101
Novel sophisticated derivatizing agents for the efficient enantioselective separation and mass spectrometric detection of - and -amino acids have been... 
Liquid chromatography | Axially chiral | Mass spectrometry | Chiral derivatization | BiAC | Chiral separation | CHEMISTRY, ANALYTICAL | HPLC ANALYSIS | HIGH-THROUGHPUT | MARFEYS REAGENT | QUANTIFICATION | PRECOLUMN DERIVATIZATION | BIOCHEMICAL RESEARCH METHODS | COMPREHENSIVE ANALYSIS | D-SERINE | RAT-BRAIN | D-ASPARTIC ACID | REVERSED-PHASE | Chemical tests and reagents | Diastereomers | Analysis | Carbamates | Amino acids | Enantiomers
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8. Full Text Amino Acid Profiles in Human Tear Fluids Analyzed by High-Performance Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry
by Nakatsukasa, Mina and Sotozono, Chie and Shimbo, Kazutaka and Ono, Nobukazu and Miyano, Hiroshi and Okano, Akira and Hamuro, Junji and Kinoshita, Shigeru
American Journal of Ophthalmology, ISSN 0002-9394, 2011, Volume 151, Issue 5, pp. 799 - 808.e1
Purpose To identify the 23 amino acid profiles in human tear fluids, and to evaluate whether the ocular disease conditions reflect the amino acid profiles.... 
Ophthalmology | CELLS | TRANSPORT | REDOX STATUS | PLASMA | GLUTAMINE | METABOLISM | MACROPHAGES | GENE-EXPRESSION | OPHTHALMOLOGY | TAURINE | PATHOLOGICAL PROGRESSION | Humans | Tears - metabolism | Corneal Diseases - metabolism | Male | Chromatography, High Pressure Liquid | Conjunctival Diseases - metabolism | Aqueous Humor - metabolism | Young Adult | Amino Acids - metabolism | Tandem Mass Spectrometry | Spectrometry, Mass, Electrospray Ionization | Aging - physiology | Stevens-Johnson Syndrome - metabolism | Female | Scleral Diseases - metabolism | Aged | Eye diseases | Amino acids | Liquid chromatography | Mass spectrometry | Proteins | Cluster analysis | Disease | Metabolites | Protein synthesis | Colleges & universities | Science | Homeostasis | Gene expression
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9. Full Text Precolumn derivatization reagents for high‐speed analysis of amines and amino acids in biological fluid using liquid chromatography/electrospray ionization tandem mass spectrometry
by Shimbo, Kazutaka and Oonuki, Takashi and Yahashi, Akihisa and Hirayama, Kazuo and Miyano, Hiroshi
Rapid Communications in Mass Spectrometry, ISSN 0951-4198, 05/2009, Volume 23, Issue 10, pp. 1483 - 1492
A rapid analytical method for amines and amino acids was developed, involving derivatization with the novel reagent 3‐aminopyridyl‐N‐hydroxysuccinimidyl... 
DIAGNOSIS | CHEMISTRY, ANALYTICAL | METABONOMICS | INHERITED DISORDERS | PLASMA | METABOLISM | SPECTROSCOPY | RATS | SEPSIS | MS/MS | TOOL | AGE | Amines - chemistry | Succinates - chemistry | Amino Acids - blood | Chromatography, High Pressure Liquid - methods | Amines - blood | Amino Acids - chemistry | Rats | Chromatography, High Pressure Liquid - instrumentation | Rats, Sprague-Dawley | Carbamates - chemistry | Spectrometry, Mass, Electrospray Ionization - instrumentation | Animals | Spectrometry, Mass, Electrospray Ionization - methods
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10. Full Text Identification of novel long chain N-acylhomoserine lactones of chain length c20 from the marine phototrophic bacterium rhodovulum sulfidophilum
by Arashida, Naoko and Shimbo, Kazutaka and Terada, Takeshi and Okimi, Takuya and Kikuchi, Yo and Hashiro, Shuhei and Umekage, So and Yasueda, Hisashi
Bioscience, Biotechnology and Biochemistry, ISSN 0916-8451, 2018, Volume 82, Issue 10, pp. 1683 - 1693
Rhodovulum sulfidophilum | C20-HSL | LC-MS/MS | N-acylhomoserine lactone (AHL) | Autoinducer
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11. Full Text Identification of novel long chain N-acylhomoserine lactones of chain length C
by Arashida, Naoko and Shimbo, Kazutaka and Terada, Takeshi and Okimi, Takuya and Kikuchi, Yo and Hashiro, Shuhei and Umekage, So and Yasueda, Hisashi
Bioscience, Biotechnology, and Biochemistry, ISSN 0916-8451, 10/2018, Volume 82, Issue 10, pp. 1683 - 1693
Gram-negative bacterial quorum sensing is mainly regulated by an extracellularly produced N-acylhomoserine lactone (AHL). AHL consists of a lactone ring and an... 
Rhodovulum sulfidophilum | HSL | N-acylhomoserine lactone (AHL) | LC-MS/MS | autoinducer | C | 20
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12. Full Text Highly sensitive quantification for human plasma-targeted metabolomics using an amine derivatization reagent
by Arashida, Naoko and Nishimoto, Rumi and Harada, Masashi and Shimbo, Kazutaka and Yamada, Naoyuki
Analytica Chimica Acta, ISSN 0003-2670, 02/2017, Volume 954, pp. 77 - 87
Amino acids and their related metabolites play important roles in various physiological processes and have consequently become biomarkers for diseases.... 
Tandem mass spectrometry | Plasma | Targeted metabolomics | Derivatization | High performance liquid chromatography | CHEMISTRY, ANALYTICAL | HMDB | ALZHEIMERS-DISEASE | DIAGNOSTIC-APPROACH | TANDEM MASS-SPECTROMETRY | AUTOMATED PRECOLUMN DERIVATIZATION | CANCER | PERFORMANCE LIQUID-CHROMATOGRAPHY | JAPANESE POPULATION | SERUM METABOLOMICS | ACID PROFILES | Amines | Metabolites | Chemical tests and reagents | Analysis | Carbamates | Amino acids | Liquid chromatography | Mass spectrometry | Arginine | Tryptophan | GABA | Ethylenediamines
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13. Full Text Combination of BCAAs and glutamine enhances dermal collagen protein synthesis in protein-malnourished rats
by Murakami, Hitoshi and Murakami, Hitoshi and Shimbo, Kazutaka and Shimbo, Kazutaka and Takino, Yoshinobu and Takino, Yoshinobu and Kobayashi, Hisamine and Kobayashi, Hisamine
Amino Acids, ISSN 0939-4451, 3/2013, Volume 44, Issue 3, pp. 969 - 976
Skin collagen decreases in protein-malnourished states. Amino acids regulate protein metabolism, glutamine stimulates collagen synthesis through the conversion... 
Life Sciences | Biochemistry, general | Analytical Chemistry | Life Sciences, general | Skin collagen synthesis | Biochemical Engineering | Proteomics | Neurobiology | Glutamine and proline metabolism | Amino acids | Protein-malnourished rat | STIMULATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | HEALTHY | TRANSLATION | MTOR | FIBROBLASTS | SKELETAL-MUSCLE | ESSENTIAL AMINO-ACIDS | SKIN | LEUCINE | Dietary Proteins - analysis | Up-Regulation | Animals | Proline - metabolism | Skin - metabolism | Amino Acids, Branched-Chain - metabolism | Humans | Glutamine - metabolism | Malnutrition - metabolism | Male | Collagen - biosynthesis | Rats, Sprague-Dawley | Synthesis | Phenylalanine | Collagens | Rats | Proline | Analysis of variance | Glutamine
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14. Full Text Importance of amino acid composition to improve skin collagen protein synthesis rates in UV-irradiated mice
by Murakami, Hitoshi and Murakami, Hitoshi and Shimbo, Kazutaka and Shimbo, Kazutaka and Inoue, Yoshiko and Inoue, Yoshiko and Takino, Yoshinobu and Takino, Yoshinobu and Kobayashi, Hisamine and Kobayashi, Hisamine
Amino Acids, ISSN 0939-4451, 6/2012, Volume 42, Issue 6, pp. 2481 - 2489
Skin collagen metabolism abnormalities induced by ultraviolet (UV) radiation are the major causes of skin photoaging. It has been shown that the one-time... 
Life Sciences | Biochemistry, general | Analytical Chemistry | Life Sciences, general | Skin collagen | Biochemical Engineering | Proteomics | Neurobiology | Amino acids | Protein synthesis rate | UV-irradiated rat | PATHWAYS | HEPATOCYTES | GLUTAMINE | BIOCHEMISTRY & MOLECULAR BIOLOGY | TRANSCRIPTION | MUSCLE | TRANSLATION | ULTRAVIOLET-IRRADIATION | FIBROBLASTS | BIOSYNTHESIS | LEUCINE | Phenylalanine - metabolism | Proline - metabolism | Amino Acids, Branched-Chain - metabolism | Skin Aging - drug effects | Glutamine - metabolism | Mice, Hairless | Skin Aging - radiation effects | Male | Tritium | Procollagen - metabolism | Animals | Ultraviolet Rays | Skin - radiation effects | Arginine - pharmacology | Amino Acids, Branched-Chain - pharmacology | Proline - pharmacology | Protein Biosynthesis - drug effects | Collagen - biosynthesis | Mice | Glutamine - pharmacology | Protein Biosynthesis - radiation effects | Arginine - metabolism | Skin - drug effects | RNA | Phenylalanine | Branched chain amino acids | Fine chemicals | Radiation | Proline | Protein biosynthesis | Cobalt | Messenger RNA | Nuclear radiation | Collagen | Skin | Glutamine | Index Medicus | Synthesis | Protein synthesis | Collagens | Irradiation | Original
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15. Biaryl axially chiral derivatizing agent for simultaneous separation and sensitive detection of proteinogenic amino acid enantiomers using liquid chromatography-tandem mass spectrometry
by Harada, Masashi and Karakawa, Sachise and Yamada, Naoyuki and Miyano, Hiroshi and Shimbo, Kazutaka
Journal of chromatography. A, 05/2019, Volume 1593, p. 91
Novel sophisticated derivatizing agents for the efficient enantioselective separation and mass spectrometric detection of d- and l-amino acids have been... 
Limit of Detection | Stereoisomerism | Amino Acids - analysis | Amino Acids - chemistry | Amino Acids - isolation & purification | Chromatography, Liquid - methods | Tandem Mass Spectrometry - methods | Carbamates - chemistry
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16. Full Text Measurement of 15 N enrichment of glutamine and urea cycle amino acids derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using liquid chromatography-tandem quadrupole mass spectrometry
by Nakamura, Hidehiro and Karakawa, Sachise and Watanabe, Akiko and Kawamata, Yasuko and Kuwahara, Tomomi and Shimbo, Kazutaka and Sakai, Ryosei
Analytical Biochemistry, ISSN 0003-2697, 05/2015, Volume 476, pp. 67 - 77
Abstract 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) is an amino acid-specific derivatizing reagent that has been used for sensitive amino acid... 
LC-MS/MS | Urea cycle amino acids | Isotopic enrichment
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17. Full Text ALTERATION OF PLASMA-FREE AMINO ACIDS (PFAAS) AND AMINES COULD BE NOVEL BLOOD-BASED BIOMARKERS FOR DETECTION OF ALZHEIMER’S DISEASE AND FUTURE PROGRESSION
by Yano, Yuki and Kawai, Nobuhiro and Muramatsu, Takahiko and Arashida, Naoko and Nishimoto, Rumi and Shimbo, Kazutaka and Kasuga, Kensaku and Miyashita, Akinori and Ikeuchi, Takeshi
Alzheimer's & Dementia, ISSN 1552-5260, 07/2018, Volume 14, Issue 7, p. P1174
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18. Full Text ALTERATION OF PLASMA-FREE AMINO ACIDS (PFAAS) AND AMINES COULD BE NOVEL BLOOD-BASED BIOMARKERS FOR DETECTION OF ALZHEIMER’S DISEASE AND FUTURE PROGRESSION
by Yano, Yuki and Kawai, Nobuhiro and Muramatsu, Takahiko and Arashida, Naoko and Nishimoto, Rumi and Shimbo, Kazutaka and Kasuga, Kensaku and Miyashita, Akinori and Ikeuchi, Takeshi
Alzheimer's & Dementia: The Journal of the Alzheimer's Association, ISSN 1552-5260, 07/2018, Volume 14, Issue 7, pp. P1174 - P1174
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19. Full Text Measurement of 15N enrichment of glutamine and urea cycle amino acids derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using liquid chromatography–tandem quadrupole mass spectrometry
by Nakamura, Hidehiro and Karakawa, Sachise and Watanabe, Akiko and Kawamata, Yasuko and Kuwahara, Tomomi and Shimbo, Kazutaka and Sakai, Ryosei
Analytical Biochemistry, ISSN 0003-2697, 05/2015, Volume 476, pp. 67 - 77
6-Aminoquinolyl- -hydroxysuccinimidyl carbamate (AQC) is an amino acid-specific derivatizing reagent that has been used for sensitive amino acid quantification... 
LC–MS/MS | Urea cycle amino acids | Isotopic enrichment
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20. Full Text Measurement of.sup.15N enrichment of glutamine and urea cycle amino acids derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate using liquid chromatography-tandem quadrupole mass spectrometry
by Nakamura, Hidehiro and Karakawa, Sachise and Watanabe, Akiko and Kawamata, Yasuko and Kuwahara, Tomomi and Shimbo, Kazutaka and Sakai, Ryosei
Analytical Biochemistry, ISSN 0003-2697, 05/2015, Volume 476, p. 67
Measurement | Urea | Carbamates |