X
Search Filters
Format Format
Subjects Subjects
Subjects Subjects
X
Sort by Item Count (A-Z)
Filter by Count
index medicus (24) 24
humans (20) 20
virology (15) 15
animals (14) 14
viruses (13) 13
cell line (12) 12
microbiology (11) 11
article (10) 10
transfection (10) 10
hiv (9) 9
mice (7) 7
virus replication (7) 7
biochemistry & molecular biology (6) 6
research article (6) 6
biology (5) 5
cell biology (5) 5
endocytosis (5) 5
human immunodeficiency virus (5) 5
infections (5) 5
macrophages (5) 5
medical research (5) 5
parasitology (5) 5
pathogenesis (5) 5
proteins (5) 5
replication (5) 5
viral infections (5) 5
virus diseases (5) 5
analysis (4) 4
calcium (4) 4
deoxyribonucleic acid--dna (4) 4
genes (4) 4
hiv infections - immunology (4) 4
hiv-1 - physiology (4) 4
human-immunodeficiency-virus (4) 4
immune response (4) 4
immune system (4) 4
infection (4) 4
kinases (4) 4
kunjin virus (4) 4
lymphocytes (4) 4
medicine (4) 4
multidisciplinary sciences (4) 4
signal transduction (4) 4
apoptosis (3) 3
calcium phosphates (3) 3
carrier proteins - metabolism (3) 3
cell culture (3) 3
dendritic cells (3) 3
dextran (3) 3
disease transmission (3) 3
flavivirus (3) 3
hiv - physiology (3) 3
hiv infections - virology (3) 3
hiv-1 (3) 3
hiv-1 - immunology (3) 3
human immunodeficiency virus--hiv (3) 3
immunology (3) 3
infectious diseases (3) 3
interferon (3) 3
leukemia (3) 3
medical colleges (3) 3
membrane (3) 3
nucleic acids (3) 3
protein (3) 3
protein binding (3) 3
reagents (3) 3
resistance (3) 3
rna interference (3) 3
science (3) 3
t-cells (3) 3
toxicity (3) 3
transcription factor ap-1 - metabolism (3) 3
type-1 (3) 3
ubiquitin-protein ligases - metabolism (3) 3
viability (3) 3
viral replication (3) 3
virus internalization (3) 3
actinomycin d (2) 2
adaptor proteins, signal transducing - genetics (2) 2
allostery (2) 2
antigen (2) 2
antiviral defense (2) 2
base sequence (2) 2
beta-galactosidase - analysis (2) 2
binding-protein (2) 2
biochemistry (2) 2
biosynthesis (2) 2
biotechnology (2) 2
burst size (2) 2
carrier proteins - genetics (2) 2
carrier proteins/metabolism (2) 2
cell cycle (2) 2
cell lines (2) 2
cell membrane - metabolism (2) 2
cell migration (2) 2
cell survival (2) 2
clinical biochemistry (2) 2
cytomegalovirus (2) 2
cytotoxicity (2) 2
dengue virus (2) 2
more...
Language Language
Publication Date Publication Date
Click on a bar to filter by decade
Slide to change publication date range


Cold Spring Harbor protocols, ISSN 1940-3402, 07/2019, Volume 2019, Issue 7, p. pdb.top096271
Electroporation is a process in which brief electrical pulses create transient pores in the plasma membrane that allow nucleic acids to enter the cellular... 
Biotechnology | Transfection | Acids | Electroporation | Nucleic acids | Optimization | Cytoplasm
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 03/2019, Volume 2019, Issue 3, pp. 184 - 187
In the past, cloned DNA was introduced into cultured eukaryotic cells chiefly by biochemical methods using either calcium phosphate or diethylaminoethyl... 
Dextran | Transfection | Calcium | Reagents | Lipids | Nucleic acids | Deoxyribonucleic acid--DNA | Calcium phosphates
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 06/2018, Volume 2018, Issue 6, pp. 462 - 464
This protocol describes viability measurements for cell cultures in a 96-well tissue culture plate using alamarBlue (resazurin). The assay can be modified to... 
Journal Article
Journal of virology, ISSN 0022-538X, 08/2019
Lymph-borne Friend murine leukemia virus (FrMLV) exploits the sentinel macrophages in the draining popliteal lymph node (pLN) to infect highly permissive... 
Journal Article
Cold Spring Harbor protocols, ISSN 1940-3402, 07/2019, Volume 2019, Issue 7, p. pdb.prot095471
Electroporation, which uses pulsed electrical fields, can be used to introduce DNA into a variety of animal cells, plant cells, and bacteria. Electroporation... 
Refractory materials | Biotechnology | Transfection | Calcium | Cell lines | Electroporation | Optimization | Deoxyribonucleic acid--DNA | Calcium phosphates | Plant cells
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 07/2018, Issue 7
Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many... 
Dextran | Transfection | Calcium | Nucleic acids | Calcium phosphates
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 12/2018, Volume 2018, Issue 12, pp. 921 - 922
Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods,... 
Viruses | Transfection | Mammalian cells
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 06/2018, Volume 2018, Issue 6, pp. 465 - 468
A common method for determining cytotoxicity is based on measuring the activity of cytoplasmic enzymes released by damaged cells. Lactate dehydrogenase (LDH)... 
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 03/2019, Volume 2019, Issue 3, pp. 215 - 223
Liposomal transfection reagents vary in their ability to transfect cell lines efficiently. Some are generalists, whereas others are best used with specific... 
Transfection | Lipofectin | Toxicity | Reagents | Cell lines | Lipids | Cations | Deoxyribonucleic acid--DNA
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 03/2019, Volume 2019, Issue 3, pp. 227 - 228
There are several methods for assaying the success of transient transfections. If a plasmid expressing β-galactosidase was used, then this histochemical... 
Galactosidase | Staining | Cell culture | Escherichia coli
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 09/2018, Volume 2018, Issue 9, pp. 693 - 698
A stable cell line is generated when transfected DNA undergoes integration into a chromosome by nonhomologous recombination. Cells that stably express... 
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 09/2018, Issue 9
A stable cell line is generated when transfected DNA undergoes integration into a chromosome by nonhomologous recombination. Cells that stably express... 
Gene amplification | Recombination | Transfection | Antibiotics | Nucleotide sequence | Antibiotic resistance | Deoxyribonucleic acid--DNA | Gene sequencing
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 07/2018, Issue 7
Here, we describe two variations on the classical DEAE-dextran transfection procedure. The first involves a brief exposure of cells to a high concentration of... 
Dextran | Transfection | Cell survival | Toxicity | Exposure
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 06/2018, Issue 6
This protocol describes viability measurements for cell cultures in a 96-well tissue culture plate using alamarBlue (resazurin). The assay can be modified to... 
Cell culture | Transfection | Reagents | Tissue culture | Viability
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 06/2018, Issue 6
Among viability assays that depend on the conversion of substrate to chromogenic product by live cells, the MTT assay is still among one of the most versatile... 
Mitochondria | Cell death | Linearity | Assaying | Conversion | Viability | Substrates | Reductase | Optical density
Journal Article
Cold Spring Harbor protocols, 07/2019
Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods,... 
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 06/2018, Issue 6
A common method for determining cytotoxicity is based on measuring the activity of cytoplasmic enzymes released by damaged cells. Lactate dehydrogenase (LDH)... 
Cell culture | Dehydrogenases | Toxicity | Cytotoxicity | Pyruvic acid | Lactate dehydrogenase | Dehydrogenase | Tetrazolium salt | L-Lactate dehydrogenase | Necrosis | NADH | Nicotinamide adenine dinucleotide | Lactic acid | Damage | Viability | Apoptosis
Journal Article
Trends in Microbiology, ISSN 0966-842X, 05/2015, Volume 23, Issue 5, pp. 289 - 295
HIV spreads more efficiently in vitro when infected cells directly contact uninfected cells to form virological synapses. A hallmark of virological synapses is... 
Journal Article
Journal of Virology, ISSN 0022-538X, 2016, Volume 90, Issue 1, pp. 308 - 316
Host restriction factor TRIM5 inhibits retroviral transduction in a species-specific manner by binding to and destabilizing the retroviral capsid lattice... 
Cell Line | Cats | Animals | Carrier Proteins - metabolism | Transduction, Genetic | Retroviridae - genetics | Signal Transduction | Humans | Retroviridae - immunology | Mice | Immunity, Innate | Transcription Factor AP-1 - metabolism
Journal Article
Annual Review of Virology, ISSN 2327-056X, 9/2019, Volume 6, Issue 1, pp. 501 - 524
Viruses are causative agents for many diseases and infect all living organisms on the planet. Development of effective therapies has relied on our ability to... 
noninvasive imaging | immune response | virus dissemination | virus infection
Journal Article
No results were found for your search.

Cannot display more than 1000 results, please narrow the terms of your search.