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Cold Spring Harbor Protocols, ISSN 1940-3402, 04/2018, Volume 2018, Issue 4, pp. 251 - 252
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 04/2018, Issue 4
This protocol describes the production of teratomas and teratocarcinomas used to determine the in vivo developmental potential of adult or embryonic tissues,... 
Surgery | Brain tumors | Embryo cells | Stem cells | Stem cell transplantation | Histology | Somites | In vivo methods and tests | Tissues | Localization | Embryos | Pluripotency
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 05/2019, Issue 5
Frequently, there is a need for fluorescent protein detection in mouse cell cultures, including embryonic stem cells or their differentiated derivatives,... 
Proteins | Microscopy | Transformed cells | Embryo cells | Green fluorescent protein | Stem cells | Fluorescence | Stem cell transplantation | Embryos
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 05/2019, Issue 5
Fluorescent proteins (FPs) are popular reporters available for gene expression detection and determination of cellular identities in the mouse. This protocol... 
Proteins | Fluorescence | Gene expression | Green fluorescent protein
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 04/2019, Issue 4
A simple cell or tissue lysate can provide a sufficient quality and amount of template DNA for polymerase chain reaction (PCR). In this protocol, a small piece... 
Polymerase chain reaction | Sodium | Tissues | Deoxyribonucleic acid--DNA | Sodium hydroxide
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 04/2019, Issue 4
DNA samples are prepared from mouse tail tips by Proteinase K digestion and subsequently extracted. The resulting preparation is suitable for use in Southern... 
Polymerase chain reaction | Endopeptidase K | Southern blotting | Proteinase | Deoxyribonucleic acid--DNA
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 10/2017, Volume 2017, Issue 10, pp. 834 - 836
Mouse embryonic fibroblasts can be reprogrammed to ES cell-like pluripotent stem cells by the forced expression of four transcription factors-OCT4, SOX2, KLF4,... 
Animals | Transfection - methods | Fibroblasts - physiology | Recombination, Genetic | Cells, Cultured | Mice | Transgenes | DNA Transposable Elements
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 10/2017, Volume 2017, Issue 10, pp. 831 - 833
Mouse embryonic fibroblasts can be reprogrammed to embryonic stem (ES) cell-like pluripotent stem cells by the forced expression of four transcription... 
Gene Transfer Techniques | Animals | Fibroblasts - physiology | Recombination, Genetic | Cells, Cultured | Mice | Electroporation - methods | Transgenes | DNA Transposable Elements
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 01/2019, Volume 2019, Issue 1, pp. 85 - 86
Often, genotyping of mouse embryos is required, and a small part, such as the yolk sac, can be used for this purpose. Here, DNA samples are prepared from... 
Proteinase | Gestation | Tissues | Embryos | Weight | Polymerase chain reaction | Yolk sac | Endopeptidase K | Genotyping | Fish | Genetic testing | Deoxyribonucleic acid--DNA | Yolk
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 12/2018, Volume 2018, Issue 12, pp. 967 - 970
To obtain ubiquitous or simply widespread transgene expression from a single stable integrant transgene is quite challenging because the random genomic... 
Integration | Rodents | Translation initiation | Doxycycline | Transgenic | Transgenes | Gene expression | Loci | Gene fusion
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 01/2018, Volume 2018, Issue 1, pp. 24 - 27
For experiments that require large numbers of preimplantation mouse embryos, such as microinjection of zygotes, gonadotropins are administered to females... 
Gonadotropins - administration & dosage | Female | Mice | Animals | Superovulation
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 03/2018, Issue 3
Fluorescent protein (FP)-expressing transgenic mice are powerful genetic resources for marking both live and fixed cells and tissues. Expression in live... 
Proteins | Visualization | Rodents | Genetic resources | Transgenic mice | Fluorescence | Embryos
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 03/2018, Volume 2018, Issue 3, pp. 171 - 172
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 01/2018, Issue 1
For experiments that require large numbers of preimplantation mouse embryos, such as microinjection of zygotes, gonadotropins are administered to females... 
Zygotes | Follicle-stimulating hormone | Chorionic gonadotropin | Microinjection | Luteinizing hormone | Pituitary (anterior) | Gonadotropins | Females | Ovulation | Embryos | Oocytes
Journal Article
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