X
Search Filters
Format Format
Subjects Subjects
Subjects Subjects
X
Sort by Item Count (A-Z)
Filter by Count
index medicus (41) 41
animals (34) 34
mice (31) 31
humans (23) 23
antennas, i.e. radio aerials (16) 16
basic electric elements (16) 16
cell biology (16) 16
electricity (16) 16
crispr (15) 15
embryonic stem-cells (15) 15
genomes (15) 15
biochemistry & molecular biology (14) 14
research (14) 14
genetics & heredity (13) 13
beer (12) 12
biochemistry (12) 12
biotechnology & applied microbiology (12) 12
chemistry (12) 12
compositions thereof (12) 12
culture media (12) 12
enzymology (12) 12
metallurgy (12) 12
microbiology (12) 12
microorganisms or enzymes (12) 12
mutation (12) 12
mutation or genetic engineering (12) 12
propagating, preserving or maintaining microorganisms (12) 12
spirits (12) 12
stem cells (12) 12
vinegar (12) 12
wine (12) 12
cancer (11) 11
cells, cultured (11) 11
genes (11) 11
genomics (11) 11
multidisciplinary sciences (11) 11
mutagenesis (11) 11
agriculture (10) 10
animal husbandry (10) 10
article (10) 10
care of birds, fishes, insects (10) 10
dna transposable elements (10) 10
fishing (10) 10
forestry (10) 10
genetic aspects (10) 10
human necessities (10) 10
hunting (10) 10
mouse (10) 10
new breeds of animals (10) 10
rearing or breeding animals, not otherwise provided for (10) 10
system (10) 10
trapping (10) 10
dna transposable elements - genetics (9) 9
embryonic stem cells - metabolism (9) 9
cells (8) 8
expression (8) 8
genetics (8) 8
physiological aspects (8) 8
deoxyribonucleic acid--dna (7) 7
gene expression (7) 7
genome (7) 7
homologous recombination (7) 7
mutagenesis, insertional (7) 7
phenotype (7) 7
base sequence (6) 6
biochemical research methods (6) 6
cell line (6) 6
cell lines (6) 6
differentiation (6) 6
female (6) 6
male (6) 6
transposons (6) 6
cell line, tumor (5) 5
chromosomal transposition (5) 5
compositions or test papers therefor (5) 5
condition-responsive control in microbiological orenzymological processes (5) 5
dna (5) 5
embryonic stem cells (5) 5
fungi (5) 5
identification (5) 5
mammalian-cells (5) 5
measuring or testing processes involving enzymes, nucleicacids or microorganisms (5) 5
mice, knockout (5) 5
mutation - genetics (5) 5
processes of preparing such compositions (5) 5
properties (5) 5
proteins (5) 5
ribonucleic acid--rna (5) 5
rodents (5) 5
science (5) 5
adult (4) 4
analysis (4) 4
bioinformatics (4) 4
biology (4) 4
biotechnology (4) 4
cell & tissue engineering (4) 4
cell differentiation (4) 4
chromatin (4) 4
clustered regularly interspaced short palindromic repeats - genetics (4) 4
crispr-cas systems (4) 4
more...
Language Language
Language Language
X
Sort by Item Count (A-Z)
Filter by Count
English (116) 116
French (14) 14
German (7) 7
Japanese (6) 6
Chinese (4) 4
Korean (1) 1
Portuguese (1) 1
more...
Publication Date Publication Date
Click on a bar to filter by decade
Slide to change publication date range


Nature Protocols, ISSN 1754-2189, 2013, Volume 8, Issue 10, pp. 2061 - 2078
  I report here a detailed protocol for seamless genome editing using the piggyBac transposon in human pluripotent stem cells (hPSCs). Recent advances in... 
Journal Article
Microbiology Spectrum, 2015, Volume 3, Issue 2, p. MDNA3
The piggyBac transposon was originally isolated from the cabbage looper moth, Trichoplusia ni, in the 1980s. Despite its early discovery and dissimilarity to... 
DNA - genetics | Mammals - genetics | Animals | Models, Biological | Recombination, Genetic | DNA - metabolism | Insecta - genetics | DNA Transposable Elements
Journal Article
Nature Methods, ISSN 1548-7091, 2009, Volume 6, Issue 5, pp. 363 - 369
Induced pluripotent stem cells (iPSCs) have been generated from somatic cells by transgenic expression of Oct4 (Pou5f1), Sox2, Klf4 and Myc. A major difficulty... 
SYSTEM | BLOCKS | BIOCHEMICAL RESEARCH METHODS | HUMAN FIBROBLASTS | INDUCTION | DIFFERENTIATION | HUMAN SOMATIC-CELLS | EXPRESSION | EFFICIENCY | RNA-Binding Proteins - genetics | Gene Expression - genetics | Homeodomain Proteins - metabolism | Humans | Alkaline Phosphatase - metabolism | Valproic Acid - pharmacology | Embryo, Mammalian - metabolism | SOXB1 Transcription Factors - metabolism | Moths - genetics | Octamer Transcription Factor-3 - genetics | Chimera - embryology | Transfection | SOXB1 Transcription Factors - genetics | Kruppel-Like Transcription Factors - metabolism | Transposases - genetics | DNA Transposable Elements - genetics | Transgenes | Cell Differentiation - physiology | Nanog Homeobox Protein | Pluripotent Stem Cells - cytology | Cell Dedifferentiation - drug effects | Repressor Proteins - genetics | Proto-Oncogene Proteins c-myc - metabolism | Genetic Vectors - genetics | Pluripotent Stem Cells - metabolism | Teratoma - pathology | Animals | Embryo, Mammalian - cytology | Fibroblasts - drug effects | Octamer Transcription Factor-3 - metabolism | Pluripotent Stem Cells - transplantation | Fibroblasts - cytology | Mice | Proto-Oncogene Proteins c-myc - genetics | Cell Dedifferentiation - genetics | Chimera - metabolism | Kruppel-Like Transcription Factors - genetics | RNA-Binding Proteins - metabolism | Physiological aspects | Transposons | Nuclear reprogramming | Research | Gene expression | Genetic engineering | Rodents | Research methodology | Stem cells | Index Medicus
Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 1/2011, Volume 108, Issue 4, pp. 1531 - 1536
Journal Article
Scientific reports, ISSN 2045-2322, 04/2018, Volume 8, Issue 1, pp. 6136 - 1
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. 
CRISPR | gRNA
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2017, Volume 7, Issue 1, pp. 7384 - 10
Genome-wide CRISPR-based knockout (CRISPR-KO) screening is an emerging technique which enables systematic genetic analysis of a cellular or molecular phenotype... 
SYSTEM | VULNERABILITIES | DNA CLEAVAGE | HUMAN GENOME | RNA | TARGET SITES | HUMAN-CELLS | MULTIDISCIPLINARY SCIENCES | GENES | SGRNA DESIGN | CAS9 | CRISPR | Genomes | Ribonucleic acid--RNA | gRNA | Genetic analysis
Journal Article
PLoS Genetics, ISSN 1553-7390, 04/2016, Volume 12, Issue 4, pp. e1005932 - e1005932
The accuracy of replicating the genetic code is fundamental. DNA repair mechanisms protect the fidelity of the genome ensuring a low error rate between... 
Young Adult | Cell Lineage | Humans | Middle Aged | Cells, Cultured | Adult | Male | Polymorphism, Single Nucleotide | Mutation | Induced Pluripotent Stem Cells - cytology | Gene mutations | Stem cells | Genetic aspects | Nucleotide sequencing | Observations | Methods | DNA sequencing | Genes | Fibroblasts | Genomes | Experiments | DNA repair | Deoxyribonucleic acid--DNA | Cancer | Index Medicus | Deoxyribonucleic acid | DNA
Journal Article
Cell Reports, ISSN 2211-1247, 07/2018, Volume 24, Issue 2, pp. 489 - 502
The genetic basis of naive pluripotency maintenance and loss is a central question in embryonic stem cell biology. Here, we deploy CRISPR-knockout-based... 
CRISPR | screening | exit from pluripotency | GATOR1 | Nprl2 | Tsc2 | Akt | naive pluripotency | mTORC2 | mTORC1 | GAP ACTIVITY | TRANSITION | TARGET | COMPLEX | GROUND-STATE PLURIPOTENCY | EMBRYONIC STEM-CELLS | GENETIC SCREENS | SELF-RENEWAL | DIFFERENTIATION | MTORC1 PATHWAY | CELL BIOLOGY
Journal Article
Journal Article
Genome Research, ISSN 1088-9051, 06/2018, Volume 28, Issue 6, pp. 846 - 858
In mouse embryonic stem cells (mESCs), the expression of provirus and endogenous retroelements is epigenetically repressed. Although many cellular factors... 
CHROMATIN | BIOCHEMISTRY & MOLECULAR BIOLOGY | TRANSPOSABLE ELEMENTS | SELF-RENEWAL | ENDOGENOUS RETROVIRUSES | ESSENTIAL GENES | HUMAN GENOME | REPRESSION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GENETICS & HEREDITY | HETEROCHROMATIN FORMATION | MORC2 | HUSH COMPLEX | Research
Journal Article