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Applied and Environmental Microbiology, ISSN 0099-2240, 04/2018, Volume 84, Issue 12, p. e00270-18
ABSTRACTInEscherichia coli, many recombinant proteins are produced in the periplasm. To direct these proteins to this compartment, they are equipped with an... 
Journal Article
Journal of Molecular Biology, ISSN 0022-2836, 02/2018, Volume 430, Issue 3, pp. 389 - 403
Tail-anchored membrane proteins (TAMPs) are relatively simple membrane proteins characterized by a single transmembrane domain (TMD) at their C-terminus.... 
membrane protein | membrane targeting | membrane insertion | tail-anchored | E. coli | Crosslinked polymers | Genetic translation | Escherichia coli | Membrane proteins
Journal Article
Cellular and molecular life sciences : CMLS, 03/2017, Volume 74, Issue 5, p. 891
Escherichia coli has been widely used for the production of recombinant proteins. To improve protein production yields in E. coli, directed engineering... 
Biological Evolution | Recombinant Proteins - biosynthesis | DNA, Bacterial - metabolism | Mutagenesis | Mutation - genetics | Escherichia coli - isolation & purification
Journal Article
JOURNAL OF MOLECULAR BIOLOGY, ISSN 0022-2836, 02/2018, Volume 430, Issue 3, pp. 389 - 403
Tail-anchored membrane proteins (TAMPs) are relatively simple membrane proteins characterized by a single transmembrane domain (TMD) at their C-terminus.... 
membrane targeting | INSERTION | COMPLEX | SECYEG | RECOGNITION | TRIGGER FACTOR | BIOCHEMISTRY & MOLECULAR BIOLOGY | tail-anchored | MEMBER | membrane protein | membrane insertion | SECRETION | E. coli | Biological Sciences | Naturvetenskap | Biologiska vetenskaper | Natural Sciences
Journal Article
Cell Reports, 03/2015, Volume 10, Issue 10, pp. 1758 - 1766
The well-established E.coli protein production strain C41(DE3) was isolated from the T7 RNA polymerase-based BL21(DE3) strain for its ability to produce... 
Journal Article
EMBO Journal, ISSN 0261-4189, 2000, Volume 19, Issue 4, pp. 542 - 549
Journal Article
FEBS Letters, ISSN 0014-5793, 07/2001, Volume 501, Issue 1, pp. 1 - 5
This review focuses on a novel, evolutionarily conserved mediator of membrane protein assembly in bacteria, mitochondria and chloroplasts. This factor is... 
Membrane protein | Translocon | Alb3 | YidC | Oxa1p
Journal Article
Microbial Cell Factories, ISSN 1475-2859, 2015, Volume 14, Issue 1, pp. 1 - 11
BACKGROUND: For membrane protein production, the Escherichia coli T7 RNA polymerase (T7 RNAP)-based protein production strain BL21(DE3) in combination with... 
Journal Article
Applied and Environmental Microbiology, ISSN 0099-2240, 06/2018, Volume 84, Issue 12, p. e00270-18
In Escherichia coli, many recombinant proteins are produced in the periplasm. To direct these proteins to this compartment, they are equipped with an... 
Translocation | Energy metabolism | Membrane vesicles | Chains | Gene expression | Metabolism | Membrane proteins | Proteins | E coli | Protein folding | Proteomics | Bacteria | Yield | Periplasm | Protein transport | Cytoplasm | Recombinant
Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 09/2008, Volume 105, Issue 38, pp. 14371 - 14376
A simple generic method for optimizing membrane protein overexpression in Escherichia coli is still lacking. We have studied the physiological response of the... 
Cytometry | Messenger RNA | RNA | Escherichia coli | Genes | Cell aggregates | Fluorescence | Genetic mutation | Western blotting | Membrane proteins | Engineering | Systems biotechnology | Proteomics | SYSTEM | systems biotechnology | CLONED GENES | MUTANTS | proteomics | MULTIDISCIPLINARY SCIENCES | PURIFICATION | engineering | BACTERIOPHAGE-T7 RNA-POLYMERASE | EXPRESSION | Gene Expression | Membrane Proteins - genetics | Escherichia coli Proteins - metabolism | Lac Operon - genetics | Membrane Fusion Proteins - biosynthesis | Escherichia coli - cytology | Promoter Regions, Genetic - genetics | Viral Proteins - metabolism | Membrane Fusion Proteins - metabolism | Membrane Proteins - biosynthesis | Membrane Transport Proteins - biosynthesis | Escherichia coli - genetics | Membrane Fusion Proteins - genetics | Membrane Transport Proteins - genetics | Escherichia coli Proteins - biosynthesis | Escherichia coli - metabolism | Escherichia coli Proteins - genetics | Membrane Transport Proteins - metabolism | Escherichia coli - growth & development | Kinetics | DNA-Directed RNA Polymerases - metabolism | Gene Expression Regulation, Bacterial | Proteome - metabolism | Genetic aspects | Research | Biotechnological microorganisms | Gene expression | Properties | Microbial biotechnology | Index Medicus | Biological Sciences | Chemistry | Naturvetenskap | Kemi | Natural Sciences | Organisk kemi | Chemical Sciences | Organic Chemistry
Journal Article
Journal of Molecular Biology, ISSN 0022-2836, 11/2012, Volume 423, Issue 4, pp. 648 - 659
BL21(DE3) is widely used to overexpress proteins. In this overexpression host, the gene encoding the target protein is located on a plasmid and is under... 
membrane protein biogenesis | membrane protein functional/structural studies | T7 RNA polymerase‐based overexpression | membrane protein production | optimization of protein expression | T7 RNA polymerase-based overexpression | SYSTEM | BIOCHEMISTRY & MOLECULAR BIOLOGY | 17 RNA polymerase-based overexpression | HOSTS | COUPLED-RECEPTORS | BACTERIOPHAGE-T7 | ENHANCE | GENES | PURIFICATION | EXPRESSION | RNA-POLYMERASE | Recombinant Fusion Proteins - biosynthesis | Promoter Regions, Genetic | Gene Expression | Bacteriophage T7 - genetics | Membrane Proteins - genetics | Viral Proteins - genetics | DNA-Directed RNA Polymerases - genetics | N-Acetylmuramoyl-L-alanine Amidase - genetics | Viral Proteins - metabolism | Membrane Proteins - biosynthesis | Escherichia coli - genetics | Escherichia coli - metabolism | Recombinant Fusion Proteins - genetics | Membrane Proteins - metabolism | DNA-Directed RNA Polymerases - metabolism | Gene Expression Regulation, Bacterial | RNA | Genes | Escherichia coli | Fluorescence | Biosynthesis | RNA polymerases | Lysozyme | Membrane proteins | Protein binding | Side effects | Plasmids | Rhamnose | Cytoplasmic membranes | Chromosomes | DNA-directed RNA polymerase | Promoters | Structure-function relationships | Biological Sciences | Naturvetenskap | Biokemi och molekylärbiologi | Biologiska vetenskaper | Biochemistry and Molecular Biology | Natural Sciences
Journal Article