Methods, ISSN 1046-2023, 11/2008, Volume 46, Issue 3, pp. 143 - 151
Our understanding of the underlying mechanisms of Ca signaling as well as our appreciation for its ubiquitous role in cellular processes has been rapidly...
Chemical calcium indicators | Microscopy | CONFOCAL MICROSCOPY | INTRACELLULAR CALCIUM | CA2+ RELEASE | BIOCHEMISTRY & MOLECULAR BIOLOGY | CARDIAC MYOCYTES | BIOCHEMICAL RESEARCH METHODS | SARCOPLASMIC-RETICULUM | RYANODINE RECEPTOR | IN-VIVO | FLUORESCENT INDICATORS | SKELETAL-MUSCLE FIBERS | ENDOPLASMIC-RETICULUM | Glycine - analogs & derivatives | Anesthesia - methods | Calcium - metabolism | Fluorescent Dyes - analysis | Imidazoles - chemistry | Calcium - analysis | Benzofurans - chemistry | Cell Compartmentation | Glycine - chemistry | Indicators and Reagents - pharmacology | Fura-2 - chemistry | Parietal Lobe - physiology | Fluoresceins - chemistry | Fluorescent Dyes - chemistry | Xanthenes - chemistry | Organic Chemicals - chemistry | Fura-2 - analogs & derivatives | Astrocytes - physiology | Animals | Calcium Signaling - drug effects | Heterocyclic Compounds, 3-Ring - chemistry | Cytosol - metabolism | Mice | Aniline Compounds - chemistry | Anesthesia - veterinary | Indoles - chemistry | Dyes and dyeing
Chemical calcium indicators | Microscopy | CONFOCAL MICROSCOPY | INTRACELLULAR CALCIUM | CA2+ RELEASE | BIOCHEMISTRY & MOLECULAR BIOLOGY | CARDIAC MYOCYTES | BIOCHEMICAL RESEARCH METHODS | SARCOPLASMIC-RETICULUM | RYANODINE RECEPTOR | IN-VIVO | FLUORESCENT INDICATORS | SKELETAL-MUSCLE FIBERS | ENDOPLASMIC-RETICULUM | Glycine - analogs & derivatives | Anesthesia - methods | Calcium - metabolism | Fluorescent Dyes - analysis | Imidazoles - chemistry | Calcium - analysis | Benzofurans - chemistry | Cell Compartmentation | Glycine - chemistry | Indicators and Reagents - pharmacology | Fura-2 - chemistry | Parietal Lobe - physiology | Fluoresceins - chemistry | Fluorescent Dyes - chemistry | Xanthenes - chemistry | Organic Chemicals - chemistry | Fura-2 - analogs & derivatives | Astrocytes - physiology | Animals | Calcium Signaling - drug effects | Heterocyclic Compounds, 3-Ring - chemistry | Cytosol - metabolism | Mice | Aniline Compounds - chemistry | Anesthesia - veterinary | Indoles - chemistry | Dyes and dyeing
Journal Article
Science, ISSN 0036-8075, 9/2011, Volume 333, Issue 6051, pp. 1888 - 1891
Engineered fluorescent protein (FP) chimeras that modulate their fluorescence in response to changes in calcium ion (Ca²⁺) concentration are powerful tools for...
Wavelengths | Neurons | Emission spectra | Imaging | REPORTS | Fluorescence | HeLa cells | Libraries | Chromophores | Histamines | Genetic screening | GCAMP2 | MULTIDISCIPLINARY SCIENCES | GREEN FLUORESCENT PROTEINS | Animals, Genetically Modified | Humans | Molecular Sequence Data | Rats | Spectrometry, Fluorescence | Calcium - analysis | Directed Molecular Evolution | Green Fluorescent Proteins - genetics | Recombinant Fusion Proteins - chemistry | Caenorhabditis elegans | Animals | Transfection | Adenosine Triphosphate - metabolism | Protein Engineering | Fluorescence Resonance Energy Transfer | Luminescent Proteins - chemistry | Luminescent Proteins - genetics | Neurons - metabolism | HeLa Cells | Green Fluorescent Proteins - chemistry | Calcium Signaling | Signal transduction | Ions | Calcium | Cellular biology | Molecular biology
Wavelengths | Neurons | Emission spectra | Imaging | REPORTS | Fluorescence | HeLa cells | Libraries | Chromophores | Histamines | Genetic screening | GCAMP2 | MULTIDISCIPLINARY SCIENCES | GREEN FLUORESCENT PROTEINS | Animals, Genetically Modified | Humans | Molecular Sequence Data | Rats | Spectrometry, Fluorescence | Calcium - analysis | Directed Molecular Evolution | Green Fluorescent Proteins - genetics | Recombinant Fusion Proteins - chemistry | Caenorhabditis elegans | Animals | Transfection | Adenosine Triphosphate - metabolism | Protein Engineering | Fluorescence Resonance Energy Transfer | Luminescent Proteins - chemistry | Luminescent Proteins - genetics | Neurons - metabolism | HeLa Cells | Green Fluorescent Proteins - chemistry | Calcium Signaling | Signal transduction | Ions | Calcium | Cellular biology | Molecular biology
Journal Article
Chemical Reviews, ISSN 0009-2665, 05/2008, Volume 108, Issue 5, pp. 1550 - 1564
NERVOUS-SYSTEM | GREEN FLUORESCENT PROTEIN | DYNAMIC-RANGE | STRUCTURAL BASIS | CARDIAC TROPONIN-C | IN-VIVO | CA2+ BINDING | NEURAL ACTIVITY | HIPPOCAMPAL-NEURONS | CHEMISTRY, MULTIDISCIPLINARY | TRANSGENIC MICE | Calcium-Binding Proteins - metabolism | Animals | Calcium - metabolism | Humans | Indicators and Reagents | Models, Genetic | Neurons - metabolism | Kinetics | Neuroplasticity | Fluorescence | Chemical properties | Research | Calcium-binding proteins | Analysis
Journal Article
Nature Neuroscience, ISSN 1097-6256, 09/2016, Volume 19, Issue 9, pp. 1142 - 1153
Experimental efforts to understand how the brain represents, stores and processes information require high-fidelity recordings of multiple different forms of...
ACTION-POTENTIALS | VOLTAGE-SENSING DOMAIN | CALCIUM IMAGING DATA | CAENORHABDITIS-ELEGANS | CELLULAR RESOLUTION | LIGHT-SHEET MICROSCOPY | IN-VIVO | NEURAL ACTIVITY | NEUROSCIENCES | CA2+ INDICATORS | GREEN FLUORESCENT PROTEINS | Neurotransmitter Agents - genetics | Synaptic Vesicles - metabolism | Animals | Calcium Signaling - physiology | Humans | Neurons - physiology | Neurotransmitter Agents - metabolism | Fluorescent Dyes - metabolism | Fluorescent Dyes - pharmacology | Brain - physiology | Synaptic Vesicles - genetics | Physiological aspects | Neurosciences | Usage | Research | Magnetic resonance imaging | Neurons
ACTION-POTENTIALS | VOLTAGE-SENSING DOMAIN | CALCIUM IMAGING DATA | CAENORHABDITIS-ELEGANS | CELLULAR RESOLUTION | LIGHT-SHEET MICROSCOPY | IN-VIVO | NEURAL ACTIVITY | NEUROSCIENCES | CA2+ INDICATORS | GREEN FLUORESCENT PROTEINS | Neurotransmitter Agents - genetics | Synaptic Vesicles - metabolism | Animals | Calcium Signaling - physiology | Humans | Neurons - physiology | Neurotransmitter Agents - metabolism | Fluorescent Dyes - metabolism | Fluorescent Dyes - pharmacology | Brain - physiology | Synaptic Vesicles - genetics | Physiological aspects | Neurosciences | Usage | Research | Magnetic resonance imaging | Neurons
Journal Article
Current Opinion in Neurobiology, ISSN 0959-4388, 2011, Volume 22, Issue 1, pp. 18 - 23
Highlights ► Recording activity from identified populations of neurons is a central goal of neuroscience. ► Genetically encoded fluorescent indicators are...
Neurology | Psychiatry | DYNAMIC-RANGE | VOLTAGE | FLUORESCENT INDICATORS | IN-VIVO | SIGNALS | FRET | PROTEINS | CALCIUM INDICATOR | PROBE | NEUROSCIENCES | CA2+ INDICATORS | Animals | Microscopy, Fluorescence, Multiphoton - trends | Voltage-Sensitive Dye Imaging - methods | Humans | Biomarkers - analysis | Neurons - physiology | Microscopy, Fluorescence, Multiphoton - methods | Neurosciences | Action potential
Neurology | Psychiatry | DYNAMIC-RANGE | VOLTAGE | FLUORESCENT INDICATORS | IN-VIVO | SIGNALS | FRET | PROTEINS | CALCIUM INDICATOR | PROBE | NEUROSCIENCES | CA2+ INDICATORS | Animals | Microscopy, Fluorescence, Multiphoton - trends | Voltage-Sensitive Dye Imaging - methods | Humans | Biomarkers - analysis | Neurons - physiology | Microscopy, Fluorescence, Multiphoton - methods | Neurosciences | Action potential
Journal Article
BMC BIOLOGY, ISSN 1741-7007, 09/2019, Volume 17, Issue 1, pp. 71 - 12
As a "holy grail" of neuroscience, optical imaging of membrane potential could enable high resolution measurements of spiking and synaptic activity in neuronal...
ACTION-POTENTIALS | NEURONAL-ACTIVITY | SENSITIVE FLUORESCENCE | PHOTOINDUCED ELECTRON-TRANSFER | IN-VIVO | CALCIUM | BIOLOGY | SYNAPTIC POTENTIALS | OPTICAL ELECTROPHYSIOLOGY | BRIGHT | MOLECULES | Protein engineering | Usage | Interdisciplinary research | Cell membranes | Research | Membranes | Firing pattern | Neurosciences | Populations | Research & development--R&D | Nervous system | Genetic code | Voltage indicators | Plasma membranes | Voltage | Membrane potential | Invertebrates | Indicators
ACTION-POTENTIALS | NEURONAL-ACTIVITY | SENSITIVE FLUORESCENCE | PHOTOINDUCED ELECTRON-TRANSFER | IN-VIVO | CALCIUM | BIOLOGY | SYNAPTIC POTENTIALS | OPTICAL ELECTROPHYSIOLOGY | BRIGHT | MOLECULES | Protein engineering | Usage | Interdisciplinary research | Cell membranes | Research | Membranes | Firing pattern | Neurosciences | Populations | Research & development--R&D | Nervous system | Genetic code | Voltage indicators | Plasma membranes | Voltage | Membrane potential | Invertebrates | Indicators
Journal Article
eLife, ISSN 2050-084X, 03/2016, Volume 5, Issue 2016
Genetically encoded calcium indicators (GEC's) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of...
CAENORHABDITIS-ELEGANS | 2-PHOTON | IN-VIVO | BIOLOGY | MOTOR CORTEX | FLUORESCENT PROTEINS | MOUSE VISUAL-CORTEX | CA2+ INDICATORS | CRE-RECOMBINASE | BRAIN | ORANGE | Usage | Neural circuitry | Physiological aspects | Genetic aspects | Diagnostic imaging | Calmodulin | Methods | Peptides | Neurons | Calcium imaging | Optics | Proteins | Insects | Mutagenesis | Plasmids | Light | Information processing | Phototoxicity | Genetics | Software | Mutation | Cytoplasm
CAENORHABDITIS-ELEGANS | 2-PHOTON | IN-VIVO | BIOLOGY | MOTOR CORTEX | FLUORESCENT PROTEINS | MOUSE VISUAL-CORTEX | CA2+ INDICATORS | CRE-RECOMBINASE | BRAIN | ORANGE | Usage | Neural circuitry | Physiological aspects | Genetic aspects | Diagnostic imaging | Calmodulin | Methods | Peptides | Neurons | Calcium imaging | Optics | Proteins | Insects | Mutagenesis | Plasmids | Light | Information processing | Phototoxicity | Genetics | Software | Mutation | Cytoplasm
Journal Article
Science, ISSN 0036-8075, 05/2019, Volume 364, Issue 6440, p. 598
Journal Article
BBA - Molecular Cell Research, ISSN 0167-4889, 07/2013, Volume 1833, Issue 7, pp. 1787 - 1797
Genetically encoded calcium ion (Ca ) indicators have become very useful and widely used tools for Ca imaging, not only in cellular models, but also in living...
Ca2 + imaging | FRET | Fluorescent proteins | Genetically encoded calcium ion indicators | Ratiometric | Intensiometric | imaging | MECHANISM | BIOCHEMISTRY & MOLECULAR BIOLOGY | SIGNALS | CALCIUM CONCENTRATIONS | GREEN FLUORESCENT PROTEINS | CELL BIOLOGY | Ca2+ imaging | IN-VIVO | PHOTOPROTEIN AEQUORIN | TRANSIENTS | NEURAL ACTIVITY | DYNAMICS | LIVING CELLS
Ca2 + imaging | FRET | Fluorescent proteins | Genetically encoded calcium ion indicators | Ratiometric | Intensiometric | imaging | MECHANISM | BIOCHEMISTRY & MOLECULAR BIOLOGY | SIGNALS | CALCIUM CONCENTRATIONS | GREEN FLUORESCENT PROTEINS | CELL BIOLOGY | Ca2+ imaging | IN-VIVO | PHOTOPROTEIN AEQUORIN | TRANSIENTS | NEURAL ACTIVITY | DYNAMICS | LIVING CELLS
Journal Article
Current Opinion in Chemical Biology, ISSN 1367-5931, 08/2017, Volume 39, pp. 1 - 10
Membrane voltages are ubiquitous throughout cell biology. Voltage is most commonly associated with excitable cells such as neurons and cardiomyocytes, although...
ACTION-POTENTIALS | ALL-OPTICAL ELECTROPHYSIOLOGY | BIOPHYSICS | ABSOLUTE MEMBRANE VOLTAGE | BIOCHEMISTRY & MOLECULAR BIOLOGY | IN-VIVO | CALCIUM | MICROBIAL RHODOPSIN | OPTOGENETIC EXCITATION | ELECTRICAL-ACTIVITY | PLASMA-MEMBRANE | SENSITIVE FLUORESCENT PROTEIN | Molecular Imaging - methods | Animals | Genetic Engineering | Electrophysiological Phenomena | Humans | Neurons | Resveratrol
ACTION-POTENTIALS | ALL-OPTICAL ELECTROPHYSIOLOGY | BIOPHYSICS | ABSOLUTE MEMBRANE VOLTAGE | BIOCHEMISTRY & MOLECULAR BIOLOGY | IN-VIVO | CALCIUM | MICROBIAL RHODOPSIN | OPTOGENETIC EXCITATION | ELECTRICAL-ACTIVITY | PLASMA-MEMBRANE | SENSITIVE FLUORESCENT PROTEIN | Molecular Imaging - methods | Animals | Genetic Engineering | Electrophysiological Phenomena | Humans | Neurons | Resveratrol
Journal Article
Angewandte Chemie International Edition, ISSN 1433-7851, 08/2018, Volume 57, Issue 34, pp. 10873 - 10878
Adenosine triphosphate (ATP) provides energy for the regulation of multiple cellular processes in living organisms. Capturing the spatiotemporal dynamics of...
protein engineering | live cell imaging | brown adipocytes | ATP | fluorescent probes | ACTIVATION | ENERGY-EXPENDITURE | INSULIN-SECRETION | BIOSENSOR | CHEMISTRY, MULTIDISCIPLINARY | LIVING CELLS | Proteins | Physiological aspects | Fluorescence | Cyclic adenylic acid | Energy metabolism | Calcium | Intracellular signalling | Fluorescent indicators | Metabolism | Organelles | Plant cells | Mitochondria | Dynamics | Indicators | Adenosine triphosphate | Cytoplasm | Calcium ions
protein engineering | live cell imaging | brown adipocytes | ATP | fluorescent probes | ACTIVATION | ENERGY-EXPENDITURE | INSULIN-SECRETION | BIOSENSOR | CHEMISTRY, MULTIDISCIPLINARY | LIVING CELLS | Proteins | Physiological aspects | Fluorescence | Cyclic adenylic acid | Energy metabolism | Calcium | Intracellular signalling | Fluorescent indicators | Metabolism | Organelles | Plant cells | Mitochondria | Dynamics | Indicators | Adenosine triphosphate | Cytoplasm | Calcium ions
Journal Article
Earth-Science Reviews, ISSN 0012-8252, 04/2015, Volume 143, pp. 161 - 186
Seagrass meadows are marine habitats with high ecological importance. Their detection in the fossil record will contribute to our understanding of the...
Faunal association | Proxy | Marine angiosperms | Seagrass meadow | (Paleo)ecology | Fossil record | POSIDONIA-OCEANICA | SPECIES COMPOSITION | CORALLINE RED ALGAE | FORAMINIFERAL ASSEMBLAGES | MIDDLE MIOCENE | CALCIUM-CARBONATE PRODUCTION | COMMUNITY STRUCTURE | GEOSCIENCES, MULTIDISCIPLINARY | MARINE GRASS BANKS | MARGINOPORA-VERTEBRALIS | GREAT-BARRIER-REEF | Fossilization | Marine | Habitats | Constrictions | Organisms | Assessments | Indicators | Deposition | Fossils
Faunal association | Proxy | Marine angiosperms | Seagrass meadow | (Paleo)ecology | Fossil record | POSIDONIA-OCEANICA | SPECIES COMPOSITION | CORALLINE RED ALGAE | FORAMINIFERAL ASSEMBLAGES | MIDDLE MIOCENE | CALCIUM-CARBONATE PRODUCTION | COMMUNITY STRUCTURE | GEOSCIENCES, MULTIDISCIPLINARY | MARINE GRASS BANKS | MARGINOPORA-VERTEBRALIS | GREAT-BARRIER-REEF | Fossilization | Marine | Habitats | Constrictions | Organisms | Assessments | Indicators | Deposition | Fossils
Journal Article