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JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN 1940-087X, 06/2019, Issue 148
Described is a simple, in vitro, dye dilution-based method for measuring antigen-specific CD4( )(+)T cell proliferation in human peripheral blood mononuclear... 
Issue 148 | CD4 | proliferation | MULTIDISCIPLINARY SCIENCES | Immunology and Infection | CFSE | sensitive | human | autoantigen | PBMC | cloning
Journal Article
Proceedings of the Royal Society B: Biological Sciences, ISSN 0962-8452, 05/2006, Volume 273, Issue 1590, pp. 1165 - 1171
Journal Article
Journal Article
Journal Article
by Tuijnenburg, Paul and Lango Allen, Hana and Burns, Siobhan O and Burns, Siobhan and Greene, Daniel and Jansen, Machiel H and Staples, Emily and Stephens, Jonathan and Carss, Keren J and Carss, Keren and Biasci, Daniele and Baxendale, Helen and Thomas, Moira and Chandra, Anita and Kiani-Alikhan, Sorena and Longhurst, Hilary and Longhurst, Hilary J and Seneviratne, Suranjith and Seneviratne, Suranjith L and Oksenhendler, Eric and Simeoni, Ilenia and de Bree, Godelieve J and Tool, Anton T.J and van Leeuwen, Ester M.M and Ebberink, Eduard H.T.M and Meijer, Alexander B and Tuna, Salih and Whitehorn, Deborah and Brown, Matthew and Turro, Ernest and Thrasher, Adrian and Thrasher, Adrian J and Smith, Kenneth G.C and Thaventhiran, James E and Thaventhiran, James and Kuijpers, Taco W and Kuijpers, Taco and Adhya, Zoe and Alachkar, Hana and Anantharachagan, Ariharan and Antrobus, Richard and Arumugakani, Gururaj and Bacchelli, Chiara and Bethune, Claire and Bibi, Shahnaz and Boardman, Barbara and Booth, Claire and Browning, Michael and Brownlie, Mary and Clifford, Hayley and Cooper, Nichola and Davies, Sophie and Dempster, John and Devlin, Lisa and Doffinger, Rainer and Drewe, Elizabeth and Edgar, David and Egner, William and El-Shanawany, Tariq and Gaspar, Bobby and Ghurye, Rohit and Gilmour, Kimberley and Goddard, Sarah and Gordins, Pavel and Grigoriadou, Sofia and Hackett, Scott and Hague, Rosie and Harper, Lorraine and Hayman, Grant and Herwadkar, Archana and Hughes, Stephen and Huissoon, Aarnoud and Jolles, Stephen and Jones, Julie and Kelleher, Peter and Klein, Nigel and Kumararatne, Dinakantha and Laffan, James and Lear, Sara and Lorenzo, Lorena and Maimaris, Jesmeen and Manson, Ania and McDermott, Elizabeth and Millar, Hazel and Mistry, Anoop and Morrisson, Valerie and Murng, Sai and Nasir, Iman and Nejentsev, Sergey and Noorani, Sadia and Ponsford, Mark and Qasim, Waseem and Quinn, Ellen and Quinti, Isabella and Richter, Alex and Samarghitean, Crina and Sargur, Ravishankar and Savic, Sinisa and Sewall, Carrock and Shackley, Fiona and ... and NIHR BioResource-Rare Dis and NIHR BioResource–Rare Diseases Consortium
The Journal of Allergy and Clinical Immunology, ISSN 0091-6749, 10/2018, Volume 142, Issue 4, pp. 1285 - 1296
Journal Article
Journal Article
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2019, Volume 1899, pp. 119 - 128
Myeloid-derived suppressor cells (MDSCs) are myeloid precursors of macrophages, dendritic cells, and neutrophils with a prominent role in the regulation of... 
CD4+ T-cell isolation | In vitro proliferation assay | CD4 | Myeloid-derived suppressor cells | Cell sorting | T-cell isolation | Carboxyfluorescein succinimidyl ester (CFSE)
Journal Article
Journal of Hepatology, ISSN 0168-8278, 2012, Volume 57, Issue 5, pp. 1044 - 1051
Background & Aims IL-17 secreting CD4 (Th17) and CD8 (Tc17) T cells have been implicated in immune-mediated liver diseases, but the molecular basis for their... 
Gastroenterology and Hepatology | Chemokine | Hepatitis | Liver | Bile ducts | Tc17 cells | Chemokine receptor | Interleukin-17 | Concanavalin A | Th17 cells | HEPATOCYTES | AUTOIMMUNE INFLAMMATION | INTERLEUKIN-22 | IL-17 | REGULATORY T-CELLS | DIFFERENTIATION | GASTROENTEROLOGY & HEPATOLOGY | LYMPHOCYTES | Chemokine CCL20 - metabolism | Receptors, CXCR3 - metabolism | Humans | Mice, Inbred C57BL | Cells, Cultured | Liver Diseases - pathology | Hepatitis - pathology | Cell Movement - physiology | Biliary Tract - pathology | Phenotype | Animals | Th17 Cells - metabolism | Biliary Tract - metabolism | Hepatitis - metabolism | Receptors, CCR6 - metabolism | Mice | Liver Diseases - metabolism | Disease Models, Animal | Th17 Cells - pathology | Employee recruitment | Interleukins | Carbon tetrachloride | Biological response modifiers | Hepatitis C virus | Hepatitis C | T cells | Mitogens | Cells | Interferon gamma | Endothelium | Th17, interleukin-17 secreting CD4 T helper cells | NASH, non-alcoholic steato-hepatitis | CCL4, carbon tetrachloride | IFN-γ, interferon gamma | Tc17, interleukin-17 secreting CD8 T helper cells | LIL, liver infiltrating lymphocytes | NL, normal liver | HSEC, hepatic sinusoidal endothelial cell | TNF-α, tumour necrosis factor-α | CFSE, carboxyfluorescein succinimidyl ester | NANB, non-A non-B acute hepatitis | ALD, alcoholic liver disease | RORC, retinoic acid-related orphan receptor c | PBC, primary biliary cirrhosis | BEC, biliary epithelial cells | HCV, chronic hepatitis C | ConA, concanavalin A | AIH, autoimmune hepatitis
Journal Article
Gastroenterology, ISSN 0016-5085, 2017, Volume 153, Issue 1, pp. 233 - 248.e16
Background & Aims There is growing interest in the use of bone marrow cells to treat liver fibrosis, however, little is known about their antifibrotic efficacy... 
Gastroenterology and Hepatology | Sphingolipid | Immune Cell Localization | Mouse Model | CCR2 | SURVIVAL | REGRESSION | HEPATOCYTES | MACROPHAGES | RESOLUTION | TRANSPLANT | TRAFFICKING | BONE-MARROW-CELLS | SPHINGOSINE | GASTROENTEROLOGY & HEPATOLOGY | EXPRESSION | Lysophospholipids - metabolism | Liver - pathology | Humans | Immunosuppressive Agents - therapeutic use | Actins - metabolism | Male | Macrophages | Lymph - metabolism | Hematopoietic Stem Cells - physiology | Female | Sphingosine - metabolism | Aldehyde-Lyases - genetics | Liver Cirrhosis - genetics | Chemical and Drug Induced Liver Injury, Chronic - complications | Fingolimod Hydrochloride - therapeutic use | Cell Line | Liver Cirrhosis - etiology | Gene Expression | Phosphoric Monoester Hydrolases - genetics | Collagen Type I - metabolism | Liver Cirrhosis - prevention & control | Lysophospholipids - antagonists & inhibitors | Membrane Proteins - genetics | Sphingosine - antagonists & inhibitors | Liver - metabolism | Neutrophils | Hematopoietic Stem Cell Transplantation | Phosphotransferases (Alcohol Group Acceptor) - genetics | Phosphotransferases (Alcohol Group Acceptor) - metabolism | Cell Movement - drug effects | Monocytes | Sphingosine - analogs & derivatives | Animals | Liver Cirrhosis - pathology | Mice | Prevention | Phosphates | Medical research | Liver diseases | Immunotherapy | Liver | Fibrosis | Sphingosine | Medicine, Experimental | Research institutes | Hematopoietic stem cells | Analysis | Bone marrow | Lipids | Transplantation | Liver cirrhosis | APC, allophycocyanin | Original Research | CFSE, carboxyfluorescein succinimidyl ester | α-SMA, α-smooth muscle actin | CCL, chemokine (C-C motif) ligand | HPC-7, hematopoietic progenitor cell line | DiR, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide | CMP, common myeloid progenitors | BM, bone marrow | CLP, common lymphoid progenitors | KSL, c-kit+ sca-1+ and lineageneg | HSC, hematopoietic stem cell | PSR, picrosirius red | S1P, sphingosine 1-phosphate | SGPL1, sphingosine-1-phosphate lyase | SGPP1, sphingosine-1-phosphate phosphatase | MMP, matrix metalloproteinase | SphK1, sphingosine kinase 1
Journal Article
FEBS Letters, ISSN 0014-5793, 05/2014, Volume 588, Issue 9, pp. 1749 - 1754
Journal Article