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NATURE COMMUNICATIONS, ISSN 2041-1723, 07/2019, Volume 10, Issue 1, pp. 3144 - 10
Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed, high-throughput screening strategy that minimizes library size without sacrificing gene targeting efficiency... 
DESIGN | SPECIFICITIES | RNA | MULTIDISCIPLINARY SCIENCES | Gene Library | RNA, Guide - chemistry | Humans | Clustered Regularly Interspaced Short Palindromic Repeats - genetics | CRISPR-Cas Systems - genetics | Gene Editing | CRISPR-Associated Protein 9 - genetics | Multiplexing | CRISPR | Screening | Gene targeting | Genomics | High-throughput screening | Libraries | Genomes
Journal Article
Nature Communications, ISSN 2041-1723, 12/2018, Volume 9, Issue 1, pp. 5416 - 15
The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference (CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene function... 
RAF INHIBITION | VULNERABILITIES | TRANSCRIPTIONAL ACTIVATION | DESIGN | CRISPR/CAS9 | SPECIFICITY | HUMAN-CELLS | MULTIDISCIPLINARY SCIENCES | KNOCKOUT | EXPRESSION | CANCER | Genomic Library | Streptococcus pyogenes | CRISPR-Associated Protein 9 | CRISPR-Cas Systems | CRISPR | RNA-mediated interference | Genes | Positive selection | Interrogation | Libraries | Genomes | Negative selection | Open reading frames | Perturbation | Genetic screening | Geckos
Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 1/2017, Volume 114, Issue 4, pp. 722 - 727
.... However, because genome-wide CRISPR libraries are mostly constructed in lentiviral vectors, in vivo applications are severely limited as a result of difficulties in delivery... 
piggyBac transposon | Liver cancer | Screening | Tumorigenesis | CRISPR/Cas9 | screening | MUTAGENESIS | MULTIDISCIPLINARY SCIENCES | MOUSE | CANCER | GENOME | CHROMOSOMAL REARRANGEMENTS | PLURIPOTENT STEM-CELLS | GENES | LIVER | TUMOR-SUPPRESSOR | liver cancer | GENERATION | tumorigenesis | Biological Sciences | CRISPR | Cas9
Journal Article
Glycobiology, ISSN 0959-6658, 05/2018, Volume 28, Issue 5, pp. 295 - 305
Over 200 glycosyltransferases are involved in the orchestration of the biosynthesis of the human glycome, which is comprised of all glycan structures found on... 
gRNA design | glycosylation | IDAA | glycoengineering | gene editing | glycome | CELLS | DESIGN | BIOCHEMISTRY & MOLECULAR BIOLOGY | CRISPR-CAS9 | DISCOVERY | GLYCOPROTEOME | NOMENCLATURE | CADHERINS | O-MANNOSYLATION | SCREENS
Journal Article
Cancer Communications, ISSN 2523-3548, 12/2016, Volume 35, Issue 1, pp. 1 - 3
...) library with a dual‐fluorescence‐based protein stability reporter and high‐throughput sequencing to screen for regulators of protein stability... 
Protein stability | Ubiquitination | Acetylation | CRISPR–Cas9 screening | Cdc25A | CRISPR-Cas9 screening | ONCOLOGY | HUMAN-CELLS | Protein Stability | Gene Library | Genome | Proteolysis | Clustered Regularly Interspaced Short Palindromic Repeats | Fluorescence | Genomics | Proteins | Researchers | Rodents | Cell cycle | Cell division | Physiology | Regulation | Genomes | Tumorigenesis
Journal Article
Frontiers in Genetics, ISSN 1664-8021, 2015, Volume 6
... endogenous gene. In principle, any genomic region can be targeted, and recent efforts have successfully generated pooled guide RNA libraries for coding and regulatory... 
High-throughput screening | CRISPR | Knockdown | High-content imaging | SiRNA | Cas9/sgRNA | OFF-TARGET SITES | SEQUENCE-SPECIFIC CONTROL | HUMAN-CELLS | CRISPR-CAS9 SYSTEM | ENDOTHELIAL-CELLS | IMAGE-ANALYSIS | GENETICS & HEREDITY | GENE-EXPRESSION | CAS SYSTEMS | ENDONUCLEASE CAS9 | RNA-INTERFERENCE | Genomics | High-Throughput Screening | siRNA | knockdown | high-content imaging
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2019, Volume 9, Issue 1, pp. 3780 - 9
.... Here, we developed a method to obtain responsive cells used in bioassay of proteins. After screening of a CRISPR/Cas9 library, we obtained a responsive cell line that grew faster in the presence of rhEGF... 
SCALE CRISPR-CAS9 KNOCKOUT | BIOACTIVITY DETERMINATION | PROTEIN | MULTIDISCIPLINARY SCIENCES | REPORTER GENE ASSAY | GROWTH | GLAND | FUNCTIONAL GENOMICS | Proteins | CRISPR | Animal models | Epidermal growth factor | Biological assays | Bioassays
Journal Article