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Cold Spring Harbor Protocols, ISSN 1940-3402, 08/2018, Issue 8
The microinjection of DNA directly into the pronuclei of fertilized zygotes is the most extensively used method of gene transfer in the mouse. The injection of... 
Yeast | Gene transfer | Transgenic mice | Regulatory sequences | Artificial chromosomes | Gene expression | Bacterial artificial chromosomes | Yeast artificial chromosomes | Zygotes | Microinjection | Chromosomes | Deoxyribonucleic acid--DNA | Plugs
Journal Article
Journal of Microbiology and Biotechnology, ISSN 1017-7825, 05/2018, Volume 28, Issue 5, pp. 821 - 825
A copy number amplification system for yeast artificial chromosomes (YACs) was combined with simultaneous overexpression of genes integrated into a YAC. The... 
Yeast artificial chromosome | Copy number amplification | PCR-mediated chromosome splitting | Simultaneous overexpression | 생물학
Journal Article
Journal of Microbiology and Biotechnology, ISSN 1017-7825, 2018, Volume 28, Issue 5, pp. 821 - 825
A copy number amplification system for yeast artificial chromosomes (YACs) was combined with simultaneous overexpression of genes integrated into a YAC. The... 
simultaneous overexpression | Yeast artificial chromosome | copy number amplification | PCR-mediated chromosome splitting
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 08/2018, Issue 8
This protocol describes a method for concentrating yeast artificial chromosome (YAC) DNA preparations following separation of chromosomes by pulsed-field gel... 
Pulsed-field gel electrophoresis | Microinjection | Purification | Ultrafiltration | Yeast | Gel electrophoresis | Electrophoresis | Artificial chromosomes | Food processing industry | Chromosomes | Deoxyribonucleic acid--DNA | Bacterial artificial chromosomes
Journal Article
Cold Spring Harbor Protocols, ISSN 1940-3402, 08/2018, Issue 8
This protocol describes a method to purify yeast artificial chromosome (YAC) DNA for microinjection. YAC DNA solutions cannot be concentrated by standard DNA... 
Precipitation | Pulsed-field gel electrophoresis | Microinjection | Purification | Yeast | Chemical precipitation | Gel electrophoresis | DNA damage | Electrophoresis | Artificial chromosomes | Deoxyribonucleic acid--DNA | Plugs
Journal Article
PLoS ONE, ISSN 1932-6203, 08/2014, Volume 9, Issue 8, p. e104995
Journal Article
Biology of Reproduction, ISSN 0006-3363, 12/2004, Volume 71, Issue 6, pp. 1943 - 1947
Journal Article
Journal Article
Science, ISSN 0036-8075, 03/2017, Volume 355, Issue 6329, pp. eaaf4791 - eaaf4791
Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates... 
REPLICATION | TRANSFER-RNA DECAY | CLONING | PATHWAY | MULTIDISCIPLINARY SCIENCES | GENES | EXPLORATION | DUPLICATION | SEQUENCING DATA | SACCHAROMYCES-CEREVISIAE | MYCOPLASMA-GENITALIUM GENOME | Chromosomes, Artificial, Yeast - chemistry | Chromosomes, Artificial, Yeast - physiology | Chromosome Segregation | Saccharomyces cerevisiae - genetics | Transcriptome | Glycerol | DNA Replication | Sequence Analysis, DNA | Chromosomes, Artificial, Yeast - genetics | Synthetic Biology | Genome, Fungal | Proteomics | Culture Media - chemistry | Saccharomyces cerevisiae - growth & development | Regulations | Profiling | Copy number | Homologous recombination | Assaying | Gene sequencing | Complexity | Design | Cellular communication | Temperature effects | Complementation | Computer networks | Chromosomes | Carbon sources | Deoxyribonucleic acid--DNA | Construction | Translation | tRNA | Meiosis | Gene expression | Replication | Anaphase | Metabolomics | Yeast | Genes | Gene regulation | Homology | Genomes | High temperature | Defects | Clonal deletion | Metaphase | Cell cycle | Deletion | Chromosome II | Evolution | Iterative methods | Integration | Osmolarity | Colonies | Debugging | Functional analysis | Ribonucleic acid--RNA | Biological activity | DNA biosynthesis | Phenotyping | Strategy | In vivo methods and tests | Index Medicus | Origins | Segregations | Glycerols
Journal Article