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Nature Communications, ISSN 2041-1723, 12/2018, Volume 9, Issue 1, pp. 1855 - 8
LbCpf1, derived from Lachnospiraceae bacterium ND2006, is a CRISPR RNA-guided endonuclease and holds promise for therapeutic applications. Here we show that... 
CELLS | SPECIFICITIES | ENDONUCLEASE | EFFICACY | INJECTION | MULTIDISCIPLINARY SCIENCES | CPF1 | CRISPR-CAS | EXPRESSION | ENDOTHELIAL GROWTH-FACTOR | NUCLEASES | Dependovirus - genetics | Endonucleases - genetics | Genetic Vectors - administration & dosage | Intravitreal Injections | RNA, Guide - genetics | Humans | Choroidal Neovascularization - therapy | Choroidal Neovascularization - etiology | Male | Vascular Endothelial Growth Factor A - genetics | Retinal Pigment Epithelium - pathology | Choroidal Neovascularization - pathology | Endonucleases - administration & dosage | Retinal Pigment Epithelium - blood supply | Clostridiales - genetics | Retinal Pigment Epithelium - radiation effects | Clustered Regularly Interspaced Short Palindromic Repeats - genetics | Disease Models, Animal | Lasers - adverse effects | Macular Degeneration - etiology | Cell Line | Specific Pathogen-Free Organisms | Hypoxia-Inducible Factor 1, alpha Subunit - genetics | Mice, Inbred C57BL | Bacterial Proteins - genetics | Treatment Outcome | Bacterial Proteins - administration & dosage | Genetic Vectors - genetics | Animals | Macular Degeneration - genetics | Macular Degeneration - therapy | Gene Editing - methods | Mice | Choroidal Neovascularization - genetics | Genetic Therapy - methods | Macular Degeneration - pathology | CRISPR | Therapeutic applications | Medical treatment | Viruses | Retina | Genomes | Ribonucleic acid--RNA | Epithelium | Age related diseases | Retinal pigment epithelium | Vascularization | Macular degeneration | Angiogenesis | Gene disruption | Hypoxia | Endonuclease | Vascular endothelial growth factor | Genetic modification | Age
Journal Article
Molecular Cell, ISSN 1097-2765, 08/2017, Volume 67, Issue 4, pp. 633 - 645.e3
The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA) and cleaves the double-stranded DNA target complementary to the crRNA... 
CRISPR-Cas | Cpf1 | crystal structure | protospacer adjacent motif | Cas12a | SYSTEM | COMPLEX | ENDONUCLEASE | DUAL-RNA | CRYSTAL-STRUCTURE | BIOCHEMISTRY & MOLECULAR BIOLOGY | CPF1 | GUIDE RNA | CAS9 | TARGET DNA RECOGNITION | REVEALS | CELL BIOLOGY | CRISPR-Associated Proteins - chemistry | Endonucleases - genetics | Nucleic Acid Heteroduplexes - genetics | RNA, Guide - genetics | RNA, Guide - metabolism | Humans | Bacterial Proteins - chemistry | Crystallography, X-Ray | Structure-Activity Relationship | Endonucleases - metabolism | Nucleic Acid Heteroduplexes - metabolism | RNA, Guide - chemistry | Clustered Regularly Interspaced Short Palindromic Repeats | HEK293 Cells | Clostridiales - genetics | Acidaminococcus - genetics | Nucleic Acid Conformation | Binding Sites | Escherichia coli - enzymology | CRISPR-Associated Proteins - genetics | Bacterial Proteins - genetics | Models, Molecular | Acidaminococcus - enzymology | DNA - metabolism | Endonucleases - chemistry | Nucleic Acid Heteroduplexes - chemistry | DNA - genetics | DNA - chemistry | Escherichia coli - genetics | CRISPR-Associated Proteins - metabolism | CRISPR-Cas Systems | Protein Binding | Bacterial Proteins - metabolism | Protein Conformation | Clostridiales - enzymology | Nucleases | DNA binding proteins | RNA | Structure | Genomics | Crystals | Enzymes
Journal Article
Nature Medicine, ISSN 1078-8956, 10/2018, Volume 24, Issue 10, pp. 1526 - 1531
Trillions of microorganisms inhabit the human gut and are regarded as potential key factors for health(1,2). Characteristics such as diet, lifestyle, or... 
MEDICINE, RESEARCH & EXPERIMENTAL | DIET | GLUCOSE-METABOLISM | GENETICS | BIOCHEMISTRY & MOLECULAR BIOLOGY | DIVERSITY | HEALTH | AGE | CELL BIOLOGY | Genetics, Population | Africa - epidemiology | Clostridiales - classification | Humans | Netherlands - epidemiology | Risk Factors | Male | Gastrointestinal Microbiome - genetics | Bacteroides - genetics | Feces - microbiology | Ethnic Groups - genetics | Microbiota - genetics | Ethnic Groups - classification | Clostridiales - genetics | RNA, Ribosomal, 16S - genetics | Female | Clostridiales - isolation & purification | Bacteroides - classification | Bacteroides - isolation & purification | Ethnicity | Composition | Microbiota (Symbiotic organisms) | Demographic aspects | Urban population | Physiological aspects | Health aspects | Geography | rRNA 16S | Digestive system | Microbiomes | Ribonucleic acid--RNA | Minority & ethnic groups | Ethnic factors | Gene sequencing | Microorganisms | Microbiota | Diet | Intestine | Genetics | Intestinal microflora | Digestive tract | Life Sciences | Santé publique et épidémiologie | Food and Nutrition | disease | Gastroenterologi | health | glucose-metabolism | enterotypes | Cell Biology | Medicine | Biochemistry & Molecular Biology | Research & Experimental | diversity | genetics | diet | markers | Gastroenterology and Hepatology | age
Journal Article
Journal Article
Applied Microbiology and Biotechnology, ISSN 0175-7598, 12/2016, Volume 100, Issue 24, pp. 10417 - 10428
The reductase component (MhpP) of the Sulfobacillus acidophilus TPY multicomponent phenol hydroxylase exhibits only 40 % similarity to Pseudomonas sp. strain... 
Life Sciences | Biotechnology | Microbiology | Sulfobacillus acidophilus TPY | Microbial Genetics and Genomics | NADPH-dependent reductase | Multicomponent phenol hydroxylase | Heterologous expression | BACTERIAL MULTICOMPONENT MONOOXYGENASES | METHYLOCOCCUS-CAPSULATUS BATH | SOLUBLE METHANE MONOOXYGENASE | PSEUDOMONAS SP STRAIN-CF600 | TOLUENE/O-XYLENE MONOOXYGENASE | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | PURIFICATION | COMPONENT | FERREDOXIN REDUCTASE | NUCLEOTIDE-SEQUENCE | PHTHALATE DIOXYGENASE REDUCTASE | Iron-Sulfur Proteins - genetics | Substrate Specificity | Mixed Function Oxygenases - metabolism | Flavin-Adenine Dinucleotide - analysis | Mixed Function Oxygenases - chemistry | Iron-Sulfur Proteins - chemistry | Oxidoreductases - chemistry | Recombinant Proteins - isolation & purification | Coenzymes - metabolism | Oxidoreductases - isolation & purification | Pseudomonas - enzymology | Clostridiales - genetics | Conserved Sequence | Mixed Function Oxygenases - isolation & purification | NADP - metabolism | Spectrum Analysis | Pseudomonas - genetics | Recombinant Proteins - metabolism | Gene Expression | Mutagenesis, Site-Directed | Oxidoreductases - metabolism | Oxidoreductases - genetics | Mutant Proteins - genetics | Recombinant Proteins - chemistry | Mutant Proteins - metabolism | Recombinant Proteins - genetics | Iron-Sulfur Proteins - isolation & purification | Amino Acid Motifs | Sequence Homology, Amino Acid | Escherichia coli - genetics | Iron-Sulfur Proteins - metabolism | Clostridiales - enzymology | Mixed Function Oxygenases - genetics | Physiological aspects | Bacillus (Bacteria) | Genetic aspects | Gene expression | Observations | NADP (Coenzyme) | Studies | Bacteria | Research | E coli | Peptides
Journal Article
Endocrinology, ISSN 0013-7227, 04/2016, Volume 157, Issue 4, pp. 1630 - 1643
Journal Article
PLOS ONE, ISSN 1932-6203, 05/2017, Volume 12, Issue 5, p. e0177735
Plant-derived protein sources are the most relevant substitutes for fishmeal in aquafeeds. Nevertheless, the effects of plant based diets on the intestinal... 
REPLACEMENT | MULTIPLE CONTRAST TESTS | ZEBRAFISH | PROTEIN | CARP | FISH-MEAL | GLUCOSE-METABOLISM | INTESTINAL MICROBIOTA | MULTIDISCIPLINARY SCIENCES | PLANT-BASED DIET | SALMO-SALAR | Gastrointestinal Microbiome - physiology | Fusobacterium - genetics | Gastrointestinal Microbiome - genetics | Alteromonadaceae - genetics | Bacteroides - genetics | Alteromonadaceae - isolation & purification | Animals | Diet | Oncorhynchus mykiss - microbiology | Bacillales - genetics | Clostridiales - genetics | Bacillales - isolation & purification | Clostridiales - isolation & purification | Bacteroides - isolation & purification | Animal Feed | DNA, Ribosomal - genetics | Fusobacterium - isolation & purification | Microbiota (Symbiotic organisms) | Trout | Analysis | Physiological aspects | Research | Health aspects | Cell culture | Carnivores | High resolution | Liver | Cellulose | Eggs | Proteins | Microorganisms | Alterations | Animal breeding | Physiological factors | Genetics | Physiology | Inhibition | Species richness | Assembly | Community structure | Carbohydrates | Enzymes | Pathogens | Starvation | Breeding | Salmon | Amino acid composition | Carbohydrate metabolism | Zebrafish | Chitin | Plants | Breeding of animals | Fermentation | Metabolism | Studies | Nutrition research | Fish diseases | Documents | Animal protein | Animal welfare | Aquaculture | Biology | Polysaccharides | Immunology | Intestine | Network analysis | Bacteria | Fish | Digestive tract | Local government | Nutrition | Histology | Feeding | Vertebrates | Amylases | Utilization | Plant breeding | Cellulase | Feeds | Colonization
Journal Article
Mikrobiologiia, ISSN 0026-3656, 07/2016, Volume 85, Issue 4, p. 472
Molecular genetic techniques (NGS sequencing and quantitative PCR) were used to determine the composition of the cecal bacterial community of broiler chickens... 
Clostridiales - classification | Lactobacillaceae - genetics | Burkholderiaceae - genetics | Cecum - microbiology | Bifidobacterium - isolation & purification | Enterococcus - isolation & purification | Staphylococcus - metabolism | Bacteria - classification | Clostridiales - genetics | Burkholderiaceae - metabolism | Bacteroidetes - isolation & purification | Bacteria - metabolism | Pasteurellaceae - genetics | Bifidobacterium - classification | Bifidobacterium - genetics | Burkholderiaceae - isolation & purification | Fermentation | Clostridiales - metabolism | Bacteroidetes - metabolism | Staphylococcus - isolation & purification | Chickens | RNA, Ribosomal, 16S - genetics | Pasteurellaceae - classification | Enterococcus - genetics | Cellulose - metabolism | Burkholderiaceae - classification | Weight Gain - physiology | Lactobacillaceae - isolation & purification | Animal Feed - analysis | Bifidobacterium - metabolism | Polymerase Chain Reaction | Rumen - microbiology | Microbial Consortia - genetics | Staphylococcus - genetics | Pasteurellaceae - isolation & purification | Staphylococcus - classification | Meat - analysis | Pasteurellaceae - metabolism | Lactobacillaceae - classification | Bacteroidetes - classification | Gastrointestinal Microbiome - genetics | Bacteria - genetics | Bacteria - isolation & purification | Animals | Clostridiales - isolation & purification | Enterococcus - metabolism | High-Throughput Nucleotide Sequencing | Bacteroidetes - genetics | Enterococcus - classification | Lactobacillaceae - metabolism
Journal Article
Journal Article