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1. Full Text Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts
by Barbosa, Oveimar and Ortiz, Claudia and Berenguer-Murcia, Ángel and Torres, Rodrigo and Rodrigues, Rafael C and Fernandez-Lafuente, Roberto
Biotechnology Advances, ISSN 0734-9750, 09/2015, Volume 33, Issue 5, pp. 435 - 456
In this review, we detail the efforts performed to couple the purification and the immobilization of industrial enzymes in a single step. The use of... 
Multimeric enzymes | IMAC | Controlled immobilization | Chimeric proteins | Covalent attachment | Enzyme stabilization | Ionic exchange | PENICILLIN-G ACYLASE | MULTIPOINT COVALENT ATTACHMENT | REVERSIBLE IMMOBILIZATION | SITE-DIRECTED MUTAGENESIS | SALT-INDUCED IMMOBILIZATION | PANCREATIC RIBONUCLEASE-A | SELECTIVE ADSORPTION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | GLYOXYL-AGAROSE | ION AFFINITY-CHROMATOGRAPHY | CHITIN-BINDING DOMAIN | Biotechnology | Enzymes, Immobilized - chemistry | Enzymes, Immobilized - metabolism | Chromatography, Ion Exchange | Proteins - metabolism | Enzyme Stability | Enzymes, Immobilized - isolation & purification | Proteins - chemistry | Proteins - isolation & purification | Enzymes | Viral antibodies | Antibodies | Index Medicus | Covalence | Immobilization | Strategy | Affinity | Joining | Silicates
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2. Full Text Modifying enzyme activity and selectivity by immobilization
by Rodrigues, Rafael C and Ortiz, Claudia and Berenguer-Murcia, Ángel and Torres, Rodrigo and Fernández-Lafuente, Roberto
Chemical Society Reviews, ISSN 0306-0012, 08/2013, Volume 42, Issue 15, pp. 6290 - 6307
Immobilization of enzymes may produce alterations in their observed activity, specificity or selectivity. Although in many cases an impoverishment of the... 
INHIBITION | KINETICALLY CONTROLLED SYNTHESIS | PENICILLIN-G | STABILIZATION | PERFORMANCE | SUPPORTS | CANDIDA-ANTARCTICA | CHEMICAL-MODIFICATION | LIPASE-B | CHEMISTRY, MULTIDISCIPLINARY | INTERFACIAL ACTIVATION | Enzymes - chemistry | Biocatalysis | Models, Molecular | Protein Conformation | Molecular Structure | Enzyme Activation | Enzymes - metabolism
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3. Modifying enzyme activity and selectivity by immobilization
by Rodrigues, Rafael C and Ortiz, Claudia and Berenguer-Murcia, Angel and Torres, Rodrigo and Fernandez-Lafuente, Roberto
Chemical Society Reviews, ISSN 0306-0012, 07/2013, Volume 42, Issue 15, pp. 6290 - 6307
Immobilization of enzymes may produce alterations in their observed activity, specificity or selectivity. Although in many cases an impoverishment of the... 
Enzymes | Alterations | Enzyme activity | Immobilization | Distortion | Activation | Selectivity | Agglomeration
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4. Full Text Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance
by Hernandez, Karel and Fernandez-Lafuente, Roberto
Enzyme and Microbial Technology, ISSN 0141-0229, 2011, Volume 48, Issue 2, pp. 107 - 122
Mutagenesis and immobilization are usually considered to be unrelated techniques with potential applications to improve protein properties. However, there are... 
Enzyme stabilization | Heterofunctional supports | Multipoint covalent attachment | Controlled immobilization | Site-directed rigidification | PENICILLIN-G ACYLASE | ONE-STEP PURIFICATION | CELLULOSE-BINDING-DOMAIN | ENGINEERED CYSTEINE RESIDUE | REVERSIBLE IMMOBILIZATION | ENZYME IMMOBILIZATION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | ION AFFINITY-CHROMATOGRAPHY | DIRECT ELECTRON-TRANSFER | MODIFIED GOLD ELECTRODES | Biotechnology - methods | Enzymes | Enzymes, Immobilized - chemistry | Enzymes, Immobilized - metabolism | Immobilized Proteins - chemistry | Mutagenesis, Site-Directed | Enzymes, Immobilized - genetics | Immobilized Proteins - genetics | Biosensing Techniques | Enzyme Stability | Immobilized Proteins - metabolism
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5. Full Text Immobilization of N‑Heterocyclic Carbene Compounds: A Synthetic Perspective
by Zhong, Rui and Lindhorst, Anja C and Groche, Florian J and Kühn, Fritz E
Chemical Reviews, ISSN 0009-2665, 02/2017, Volume 117, Issue 3, pp. 1970 - 2058
Over the course of the past 15 years the success story of N-heterocyclic carbene (NHC) compounds in organic, inorganic, and organometallic chemistry has been... 
PD-NHC COMPLEXES | CONTROLLED REVERSIBLE IMMOBILIZATION | SELF-ASSEMBLED MONOLAYERS | CROSS-COUPLING REACTION | RING-CLOSING METATHESIS | TAGGED RUTHENIUM COMPLEX | 2ND-GENERATION HOVEYDA-GRUBBS | METAL-ORGANIC FRAMEWORK | HYBRID SILICA MATERIALS | SUPPORTED PALLADIUM CATALYSTS | CHEMISTRY, MULTIDISCIPLINARY
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6. Full Text Immobilisation and application of lipases in organic media
by Adlercreutz, Patrick and Bioteknik and Lund University and Biotechnology and Lunds universitet
Chemical Society Reviews, ISSN 0306-0012, 7/2013, Volume 42, Issue 15, pp. 646 - 6436
Different methods of preparing lipases for use in organic media are critically reviewed. Solid lipase preparations can be made by typical immobilisation... 
FATTY-ACID ESTERS | CONTROLLED WATER ACTIVITY | SURFACTANT-COATED LIPASE | SUPERCRITICAL CARBON-DIOXIDE | RHIZOMUCOR-MIEHEI LIPASE | LINKED ENZYME CRYSTALS | BURKHOLDERIA-CEPACIA LIPASE | CHEMISTRY, MULTIDISCIPLINARY | CANDIDA-ANTARCTICA LIPASE | DYNAMIC KINETIC RESOLUTION | THERMOMYCES-LANUGINOSA LIPASE | Enzymes, Immobilized - chemistry | Enzymes, Immobilized - metabolism | Organic Chemicals - chemistry | Hydrophobic and Hydrophilic Interactions | Models, Molecular | Organic Chemicals - metabolism | Protein Conformation | Lipase - metabolism | Lipase - chemistry | Enrichment | Enzymes | Media | Crosslinking | Activation | Surfactants | Lipases | Mass transfer | Industrial Biotechnology | Engineering and Technology | Industriell bioteknik | Teknik
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7. Full Text Chitosan–halloysite hybrid-nanotubes: Horseradish peroxidase immobilization and applications in phenol removal
by Zhai, Rui and Zhang, Bing and Wan, Yazhen and Li, Congcong and Wang, Jingtao and Liu, Jindun
Chemical Engineering Journal, ISSN 1385-8947, 01/2013, Volume 214, pp. 304 - 309
► Assembling chitosan onto the halloysite to form hybrid-nanotubes. ► High enzyme loading through covalent binding method. ► About 100% retention rate of... 
Horseradish peroxidase (HRP) | Halloysite nanotubes | Chitosan | Phenol removal | CLAY NANOTUBES | FE3O4 NANOPARTICLES | CONTROLLED-RELEASE | ENZYME IMMOBILIZATION | CARBON NANOTUBES | AQUEOUS-SOLUTION | ENGINEERING, CHEMICAL | ENGINEERING, ENVIRONMENTAL | BIOCATALYST | HYDROGEN-PEROXIDE | LIPASE | SILICA | Phenols | Peroxidase
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8. Full Text Progress in enzyme immobilization in ordered mesoporous materials and related applications
by Zhou, Zhou and Zhou, Zhou and Hartmann, Martin and Hartmann, Martin
Chemical Society Reviews, ISSN 0306-0012, 04/2013, Volume 42, Issue 9, pp. 3894 - 3912
A short time after the discovery of ordered mesoporous materials, which possess unique features such as high specific surface area and pore volume, highly... 
CYTOCHROME-C | THIN-FILMS | CARBON MOLECULAR-SIEVES | ELECTRON-TRANSFER | SILICA NANOPARTICLES | CONTROLLED-RELEASE | CATALYTIC ACTIVITY | NANOPOROUS CARBON | DIRECT ELECTROCHEMISTRY | BETA-GLUCOSIDASE | CHEMISTRY, MULTIDISCIPLINARY | Enzymes - chemistry | Particle Size | Enzymes, Immobilized - chemistry | Enzymes, Immobilized - metabolism | Biocatalysis | Surface Properties | Porosity | Enzymes - metabolism | Carriers | Enzymes | Pore size | Specific surface | Immobilization | Trends
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