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1. Full Text Correlated light and electron microscopy: Ultrastructure lights up
by De Boer, Pascal and Hoogenboom, Jacob P and Giepmans, Ben N.G
Nature Methods, ISSN 1548-7091, 05/2015, Volume 12, Issue 6, pp. 503 - 513
Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek observed living microorganisms and cells in 1674 using his light... 
SPATIAL PRECISION | FLUORESCENCE MICROSCOPY | SUPERRESOLUTION FLUORESCENCE | HIGH-RESOLUTION | INTEGRATED LIGHT | BIOCHEMICAL RESEARCH METHODS | CRYOELECTRON TOMOGRAPHY | EUKARYOTIC CELLS | MAMMALIAN-CELLS | CRYO-FLUORESCENCE | FIDUCIAL MARKERS | Cells - ultrastructure | Animals | Microscopy, Electron - methods | Microscopy, Fluorescence - methods | Humans | Staining and Labeling | Electron microscopy | Comparative analysis | Methods | Fluorescence microscopy | Microscopy | Biology
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2. Full Text Cryo-electron microscopy shapes up
by Baker, Monya
Nature, ISSN 0028-0836, 09/2018, Volume 561, Issue 7724, pp. 565 - 567
[...]many veteran crystallographers are giving up on crystals and freezing proteins for cryo-EM instead. CRITERIA CREATION The surge in cryo-EM is largely a... 
Microscopy | Structural biology | Biological techniques | MODELS | CRYO-EM MAPS | MULTIDISCIPLINARY SCIENCES | Electron microscopy | Innovations | Proteins | Medical research | Maps | Researchers | Noise | Biology | Molecular biology | Crystallography | Biologists | Freezing
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3. Full Text A Primer to Single-Particle Cryo-Electron Microscopy
by Cheng, Yifan and Grigorieff, Nikolaus and Penczek, Pawel A and Walz, Thomas
Cell, ISSN 0092-8674, 04/2015, Volume 161, Issue 3, pp. 438 - 449
Cryo-electron microscopy (cryo-EM) of single-particle specimens is used to determine the structure of proteins and macromolecular complexes without the need... 
3-DIMENSIONAL RECONSTRUCTION | MICROGRAPHS | BIOCHEMISTRY & MOLECULAR BIOLOGY | ESCHERICHIA-COLI | RESOLUTION | HIV-1 ENVELOPE GLYCOPROTEINS | BEAM-INDUCED MOTION | CRYO-EM IMAGES | CRYOMICROSCOPY | MOLECULAR ELECTRON-MICROSCOPY | CONTRAST TRANSFER | CELL BIOLOGY | Cryoelectron Microscopy - instrumentation | Algorithms | Image Processing, Computer-Assisted | Molecular Conformation | Models, Molecular | Protein Conformation | Proteins - chemistry | Proteins - isolation & purification | Cryoelectron Microscopy - methods | Proteins - ultrastructure | Proteins | Electron microscopy | Analysis | Cells | Atoms
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4. Full Text MotionCor2: Anisotropic correction of beam-induced motion for improved cryo-electron microscopy
by Zheng, Shawn Q and Palovcak, Eugene and Armache, Jean-Paul and Verba, Kliment A and Cheng, Yifan and Agard, David A
Nature Methods, ISSN 1548-7091, 2017, Volume 14, Issue 4, pp. 331 - 332
CRYO-EM | BIOCHEMICAL RESEARCH METHODS | Electron microscopy
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5. Full Text Unravelling biological macromolecules with cryo-electron microscopy
by Fernandez-Leiro, Rafael and Scheres, Sjors H.W
Nature, ISSN 0028-0836, 09/2016, Volume 537, Issue 7620, pp. 339 - 346
Knowledge of the three-dimensional structures of proteins and other biological macromolecules often aids understanding of how they perform complicated tasks in... 
RNA-POLYMERASE-II | ANGSTROM RESOLUTION | U4/U6.U5 TRI-SNRNP | ELECTRON CRYOMICROSCOPY | ATOMIC-STRUCTURE | MULTIDISCIPLINARY SCIENCES | HIV-1 ENVELOPE TRIMER | MOLECULAR ARCHITECTURE | BEAM-INDUCED MOTION | MITOCHONDRIAL RIBOSOME | CRYO-EM STRUCTURE | Multiprotein Complexes - ultrastructure | Multiprotein Complexes - chemistry | Membrane Proteins - chemistry | Membrane Proteins - ultrastructure | Humans | Models, Molecular | Cryoelectron Microscopy - trends | Biological research | Research | Molecular biology | Cryoelectron microscopy | Methods | Biology, Experimental | Proteins | Molecules | Algorithms | Nuclear magnetic resonance--NMR | Microscopy | Biology | Crystallography
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6. Full Text New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography
by Schorb, Martin and Gaechter, Leander and Avinoam, Ori and Sieckmann, Frank and Clarke, Mairi and Bebeacua, Cecilia and Bykov, Yury S and Sonnen, Andreas F.-P and Lihl, Reinhard and Briggs, John A.G
Journal of Structural Biology, ISSN 1047-8477, 02/2017, Volume 197, Issue 2, pp. 83 - 93
Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an electron micrograph of the same... 
Correlative light and electron microscopy | Cryo-electron tomography | Cryo-electron microscopy | Cryo-fluorescence microscopy | TOMOGRAPHY | SUPERRESOLUTION | VISUALIZATION | CELLS | SPATIAL PRECISION | BIOCHEMISTRY & MOLECULAR BIOLOGY | SENSITIVITY | SECTIONS | CrIfo-fluorescence microscopy | ELECTRON-MICROSCOPY | CELL BIOLOGY | STRUCTURAL BIOLOGY | BIOPHYSICS | LIGHT-MICROSCOPY | Microscopy - methods | Cryoelectron Microscopy - instrumentation | Microscopy, Fluorescence - methods | Microscopy - instrumentation | Electron Microscope Tomography - methods | Software | Electron Microscope Tomography - instrumentation | Cryoelectron Microscopy - methods | Microscopy, Fluorescence - instrumentation | Fluorescence | Electron microscopy | Molecular biology | Fluorescence microscopy | POI, position(s) of interest | FOV, field of view | LN, liquid nitrogen | NA, numerical aperture | CEMOVIS, cryo-electron microscopy of vitrified sections | TEM, transmission electron microscopy | EM, electron microscopy | WD, working distance | FM, fluorescence microscopy | ET, electron tomography | CLEM, correlative light and electron microscopy
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7. Full Text Outcome of the First Electron Microscopy Validation Task Force Meeting
by Henderson, Richard and Sali, Andrej and Baker, Matthew L and Carragher, Bridget and Devkota, Batsal and Downing, Kenneth H and Egelman, Edward H and Feng, Zukang and Frank, Joachim and Grigorieff, Nikolaus and Jiang, Wen and Ludtke, Steven J and Medalia, Ohad and Penczek, Pawel A and Rosenthal, Peter B and Rossmann, Michael G and Schmid, Michael F and Schröder, Gunnar F and Steven, Alasdair C and Stokes, David L and Westbrook, John D and Wriggers, Willy and Yang, Huanwang and Young, Jasmine and Berman, Helen M and Chiu, Wah and Kleywegt, Gerard J and Lawson, Catherine L
Structure, ISSN 0969-2126, 02/2012, Volume 20, Issue 2, pp. 205 - 214
This Meeting Review describes the proceedings and conclusions from the inaugural meeting of the Electron Microscopy Validation Task Force organized by the... 
ESCHERICHIA-COLI RIBOSOME | RESOLUTION DENSITY MAPS | CRYOELECTRON MICROSCOPY | SINGLE-PARTICLE | BIOCHEMISTRY & MOLECULAR BIOLOGY | CRYO-EM | GROUP-II CHAPERONIN | INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR | CELL BIOLOGY | 3-DIMENSIONAL STRUCTURE | HERPES-SIMPLEX-VIRUS | BIOPHYSICS | MACROMOLECULAR ASSEMBLIES | Microscopy, Electron - standards | Guidelines as Topic | Animals | Macromolecular Substances - chemistry | Microscopy, Electron - methods | Humans | Molecular Conformation | Molecular Sequence Annotation | Databases, Factual - statistics & numerical data | Information Storage and Retrieval | Models, Molecular | Meeting Review
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8. Full Text Multi-pass transmission electron microscopy
by Juffmann, Thomas and Koppell, Stewart A and Klopfer, Brannon B and Ophus, Colin and Glaeser, Robert M and Kasevich, Mark A and Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Scientific Reports, ISSN 2045-2322, 12/2017, Volume 7, Issue 1, pp. 1699 - 7
Feynman once asked physicists to build better electron microscopes to be able to watch biology at work. While electron microscopes can now provide atomic... 
CRYO-EM | LIMIT | PHASE-CONTRAST | RADIATION-DAMAGE | PLATE | MULTIDISCIPLINARY SCIENCES | Electron microscopes | Transmission electron microscopy | Microscopy | Microscopes | OTHER INSTRUMENTATION | cryoelectron microscopy | transmission electron microscopy | BASIC BIOLOGICAL SCIENCES | quantum metrology
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9. Full Text Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer
by Walls, Alexana C and Tortorici, M Alejana and Bosch, Berend-Jan and Frenz, Brandon and Rottier, Peter J M and DiMaio, Frank and Rey, Félix A and Veesler, David
Nature, ISSN 0028-0836, 03/2016, Volume 531, Issue 7592, pp. 114 - 117
The tremendous pandemic potential of coronaviruses was demonstrated twice in the past few decades by two global outbreaks of deadly pneumonia. Entry of... 
electron microscopy | antibodies | viral vaccines | Coronavirus spike glycoprotein | MURINE CORONAVIRUS | MEMBRANE-FUSION | ELECTRON CRYOMICROSCOPY | PARTICLE CRYO-EM | CRYSTAL-STRUCTURE | MULTIDISCIPLINARY SCIENCES | FUSION PROTEIN | EM STRUCTURE DETERMINATION | HUMAN RECEPTOR | S2 DOMAIN | RESPIRATORY SYNDROME CORONAVIRUS | Protein Structure, Tertiary | Amino Acid Sequence | Cell Line | Protein Multimerization | Models, Molecular | Molecular Sequence Data | Coronavirus Infections - immunology | Virus Internalization | Cryoelectron Microscopy | Murine hepatitis virus - immunology | Antibodies, Neutralizing - immunology | Murine hepatitis virus - chemistry | Animals | Coronavirus Infections - virology | Viral Vaccines - chemistry | Spike Glycoprotein, Coronavirus - immunology | Mice | Drosophila melanogaster | Spike Glycoprotein, Coronavirus - chemistry | Viral Vaccines - immunology | Murine hepatitis virus - ultrastructure | Spike Glycoprotein, Coronavirus - ultrastructure | Physiological aspects | Glycoproteins | Coronaviruses | Electron microscopy | Structure | Methods | Immunoglobulins | Peptides | Microscopy | Respiratory diseases | Viruses | Biochemistry | Cristallography | Chemical Sciences
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10. Full Text Fibril structure of amyloid-beta(1-42) by cryo-electron microscopy
by Gremer, Lothar and Schoelzel, Daniel and Schenk, Carla and Reinartz, Elke and Labahn, Joerg and Ravelli, Raimond B. G and Tusche, Markus and Lopez-Iglesias, Carmen and Hoyer, Wolfgang and Heise, Henrike and Willbold, Dieter and Schroeder, Gunnar F
Science, ISSN 0036-8075, 10/2017, Volume 358, Issue 6359, pp. 116 - 119
Amyloids are implicated in neurodegenerative diseases. Fibrillar aggregates of the amyloid-beta protein (A beta) are the main component of the senile plaques... 
CRYO-EM | POLYMORPHISM | ATOMIC-RESOLUTION STRUCTURE | ALZHEIMERS-DISEASE | BETA-AMYLOID FIBRILS | DIMER | MULTIDISCIPLINARY SCIENCES | AMYLOID-BETA FIBRILS | Cryoelectron Microscopy | Peptide Fragments - ultrastructure | Peptide Fragments - chemistry | Protein Structure, Secondary | Amyloid beta-Peptides - ultrastructure | X-Ray Diffraction | Humans | Protein Multimerization | Nuclear Magnetic Resonance, Biomolecular | Amyloid beta-Peptides - chemistry
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11. Full Text Time-resolved cryo-electron microscopy: Recent progress
by Frank, Joachim
Journal of Structural Biology, ISSN 1047-8477, 12/2017, Volume 200, Issue 3, pp. 303 - 306
Time-resolved cryo-electron microscopy (cryo-EM) combines the known advantages of single-particle cryo-EM in visualizing molecular structure with the ability... 
Short-lived states | Cryo-EM | Microfluidics | RRF | Ribosome recycling | ELECTRON CRYOMICROSCOPY | BIOPHYSICS | BIOCHEMISTRY & MOLECULAR BIOLOGY | DYNAMICS | RIBOSOME | SUBUNIT | CELL BIOLOGY | Models, Molecular | Ribosomes - metabolism | Cryoelectron Microscopy - instrumentation | Cryoelectron Microscopy - methods | Equipment Design | Ribosomes - chemistry | Electron microscopy | Molecular biology
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12. Scanning electron microscopy
by Fischer, Elizabeth R and Hansen, Bryan T and Nair, Vinod and Hoyt, Forrest H and Dorward, David W
Current Protocols in Microbiology, ISSN 1934-8525, 05/2012, Volume Chapter 2, Issue 25, p. Unit 2B.2.
Scanning electron microscopy (SEM) remains distinct in its ability to allow topographical visualization of structures. Key elements to consider for successful... 
Immune-labeling | Scanning electron microscopy | Critical-point drying | EM specimen preparation | Microwave-processing | Cryo-SEM quantum dots | Sputter coating | Specimen fracturing | Biomedical Research - methods | Microscopy, Electron, Scanning - methods | Image Processing, Computer-Assisted - methods | Microscopy, Electron, Scanning - instrumentation | Biomedical Research - instrumentation | Specimen Handling - methods
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13. Full Text Quantitative characterization of electron detectors for transmission electron microscopy
by Ruskin, Rachel S and Yu, Zhiheng and Grigorieff, Nikolaus
Journal of Structural Biology, ISSN 1047-8477, 12/2013, Volume 184, Issue 3, pp. 385 - 393
A new generation of direct electron detectors for transmission electron microscopy (TEM) promises significant improvement over previous detectors in terms of... 
Direct electron detector | DQE | Transmission electron microscopy | MTF | CCD CAMERAS | PERFORMANCE | BIOCHEMISTRY & MOLECULAR BIOLOGY | RESOLUTION | CRYO-EM | CLASSIFICATION | BEAM-INDUCED MOTION | CRYOMICROSCOPY | CELL BIOLOGY | HETEROGENEITY | BIOPHYSICS | NOISE TRANSFER | EFFICIENCY | Microscopy, Electron, Transmission - instrumentation | Software | Image Processing, Computer-Assisted - methods | Microscopy, Electron, Transmission - methods | Electrons | Electron microscopy | Detectors | direct electron detector | transmission electron microscopy
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