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Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1817, pp. 123 - 135
The in vitro long-term expansion of primary intestinal epithelial cells has been hampered by the inability to maintain an immature stem cell population. Recent... 
Intestine tissue | Organoid | Lgr5 | Long-term culture | Intestinal epithelial cell | Crypt isolation | Adult Stem Cells - cytology | Animals | Cryopreservation | Intercellular Signaling Peptides and Proteins - pharmacology | Organoids - cytology | Mice | Culture Media - chemistry | Tissue Culture Techniques - methods | Intestines - cytology
Journal Article
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2019, Volume 1576, pp. 171 - 181
Colonic organoids, three-dimensional colonic crypts grown in vitro that show realistic microanatomy, have many potential applications for studying physiology,... 
In vitro primary culture | Niche-independent crypt expansion | Stem cells
Journal Article
Equine Veterinary Journal, ISSN 0425-1644, 03/2018, Volume 50, Issue 2, pp. 241 - 248
Summary Background Intestinal epithelial stem cells are critical to epithelial repair following gastrointestinal injury. The culture of intestinal stem cells... 
horse | enteroid | 3D culture | intestine | stem cell | organoid | ANIMALS | SYSTEM | PHYSIOLOGY | MARKER | NICHE | POPULATIONS | COLON | ORGANOIDS | IN-VITRO | DISEASE | VETERINARY SCIENCES | Intestinal Mucosa - cytology | Animals | Models, Biological | Cell Culture Techniques - veterinary | Horses | Female | Male | Stem Cells - physiology | Cell Differentiation | Stem cell research | Stem cells | Euthanasia
Journal Article
Molecular Cell, ISSN 1097-2765, 12/2016, Volume 64, Issue 5, pp. 900 - 912
Journal Article
by Li, J and Li, J and Zhang, S Y and Li, R X and Lin, X and Mi, Y L and Zhang, C Q
Poultry Science, ISSN 0032-5791, 05/2018, Volume 97, Issue 5, pp. 1536 - 1543
Abstract The integrity and normal function of the small intestinal epithelium depends critically on the rapid renewal of epithelial cells from basal stem... 
Organoid | Chicken | Crypt | Intestine | Culture | chicken | STEM-CELLS | AGRICULTURE, DAIRY & ANIMAL SCIENCE | intestine | TRANSPORTER | CANCER CELLS | MARKER | crypt | DEVELOPMENTAL REGULATION | IDENTIFICATION | ORGANOIDS | organoid | IN-VITRO | EPITHELIUM | EMBRYO | culture
Journal Article
Cytotechnology, ISSN 0920-9069, 6/2018, Volume 70, Issue 3, pp. 1085 - 1095
Recently organoids have become widely used in vitro models of many tissue and organs. These type of structures, originated from embryonic or adult mammalian... 
Biomedicine, general | Biochemistry, general | Biotechnology | Three-dimensional cell culture | Chemistry | Enterocytes | Organoids | Intestine | Chicken epithelial cells | Spheroids | STEM-CELLS | MOUSE | NICHE | CELL BIOLOGY | IN-VITRO | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | VILLUS | CRYPT | LGR5 | Proteins | Analysis | Stem cells | Method in Cell Science
Journal Article
PLoS ONE, ISSN 1932-6203, 06/2013, Volume 8, Issue 6, p. e66465
Significant advances in intestinal stem cell biology have been made in murine models; however, anatomical and physiological differences between mice and humans... 
SMALL-BOWEL RESECTION | IN-VITRO | MOUSE SMALL INTESTINE | BRUSH-BORDER | SECRETORY-CELL | MULTIDISCIPLINARY SCIENCES | GLUCAGON-LIKE PEPTIDE-2 | GASTROINTESTINAL-TRACT | BARRIER FUNCTION | PANETH CELLS | GROWTH-FACTOR | Biomarkers - metabolism | Cell Proliferation | Intestinal Mucosa - ultrastructure | Cells, Cultured | Epithelial Cells - ultrastructure | Intestinal Mucosa - physiology | Cell Lineage | Epithelial Cells - physiology | Goblet Cells - ultrastructure | Regeneration | Animals | Cell Cycle | Goblet Cells - physiology | Stem Cells - physiology | Cell Differentiation | Cell Culture Techniques | Sus scrofa | Apoptosis | Viral antibodies | Stem cell research | Swine | Mucins | Stem cells | Gastrin | Antibodies | Gene expression | Cell culture | Carbonic anhydrase II | Animal models | Disease | Epithelial cells | Sox9 protein | Bromodeoxyuridine | Absorptivity | Small intestine | Ischemia | Intestine | Rodents | Proliferating cell nuclear antigen | Cell cycle | Physiology | Bioindicators | Candidate species | Somatostatin | Biomedical engineering | Translation | Goblet cells | Mortality | Cell division | Cell lineage | Permeability | Electron microscopy | Epithelium | Medicine | Studies | Hogs | Enterocytes | Transmission electron microscopy | Mucin | Carbonic anhydrase | Crypts | Reagents | Cells (biology) | Biomarkers | Mice | Histone H3
Journal Article
Drug Metabolism and Disposition, ISSN 0090-9556, 07/2017, Volume 45, Issue 7, p. 748
The gastrointestinal tract is enriched with xenobiotic processing proteins that play important roles in xenobiotic bioactivation, metabolism, and... 
Cell culture | Camptothecin | Animal models | Toxicity | Epithelial cells | Genes | Cytotoxicity | Pregnenolone | Proteins | Metabolites | Organoids | Intestine | Rodents | Drug metabolism | Biocompatibility | Genetic modification | Cyp3a11 gene | Detoxification | Cultures | Gastrointestinal tract | Metabolism | Gene expression | Polymerase chain reaction | Crypts | Mice | Three dimensional models | Transporter | Apoptosis
Journal Article
Anticancer research, ISSN 0250-7005, 2014, Volume 34, Issue 8, pp. 4551 - 4555
Background: We have previously reported the crucial roles of oncogenic Kirsten rat sarcoma viral oncogene homolog (KRAS) in inhibiting apoptosis and disrupting... 
Spheroid | 3D culture | Luminal apoptosis | Colorectal cancer | Resveratrol | colorectal cancer | MIR-200C | PROLIFERATION | spheroid | PDE4 INHIBITORS | ROLIPRAM | DISEASES | ACTIVATED KI-RAS | ONCOLOGY | GROWTH | KRAS | EXPRESSION | COLONIC-CRYPT MODEL | luminal apoptosis | Stilbenes - pharmacology | Phosphodiesterase 4 Inhibitors - pharmacology | Apoptosis - drug effects | HCT116 Cells | Humans
Journal Article