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detergent-free isolation (48) 48
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Protein Expression and Purification, ISSN 1046-5928, 12/2018, Volume 152, pp. 146 - 154
Parkinson's disease (PD) is multifactorial, likely resulting from an intricate relationship of genetic and environmental factors affecting fundamental cellular... 
Mild purification | Tetrameric α-synuclein | Dynamic protein | Noncovalent multimer | N-terminal acetylation | Detergent-free | CELLS | PROTEIN | TERMINAL ACETYLATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | MEMBRANES | FORMS | IN-VITRO | IMPACT | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | DISEASE | BINDING | Tetrameric alpha-synuclein | AGGREGATION
Journal Article
Journal Article
Turkish Journal of Biochemistry, ISSN 0250-4685, 06/2017, Volume 42, Issue 3, pp. 287 - 297
Objective: The membranes of plant and animal cells contain lipid-protein microdomains (LPMs) in their structure. We aimed to compare biochemical and... 
Tonoplast | Detergent technique | Lipid-protein mikro alanları | Deterjan içermeyen teknik | Lipid-protein microdomains | Detergent-free technique | Deterjan tekniği | Gravimetrik yöntem | Gravimetric method | PLASMA-MEMBRANES | BIOCHEMISTRY & MOLECULAR BIOLOGY | PLANT-CELLS | MODEL | RAFTS | METABOLISM | PURIFICATION | PHOSPHATIDIC-ACID
Journal Article
Chemical Communications, ISSN 1359-7345, 2017, Volume 53, Issue 78, pp. 10824 - 10826
Polymer lipid nanodiscs have enabled some exciting structural biology and nanobiotechnology applications. The use of a small molecular weight polymer (SMA-EA)... 
NANOPARTICLES | NMR | SOLUBILIZATION | SIZE | DETERGENT-FREE ISOLATION | MODEL | MEMBRANE-PROTEINS | RECONSTITUTION | CHEMISTRY, MULTIDISCIPLINARY
Journal Article
CURRENT OPINION IN BIOTECHNOLOGY, ISSN 0958-1669, 08/2017, Volume 46, pp. 51 - 56
A plethora of polymer-based scaffolds have been designed to facilitate biochemical and biophysical investigation of membrane proteins, with a common goal to... 
CHANNEL | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | SMALPS | BIOCHEMICAL RESEARCH METHODS | DETERGENT-FREE ISOLATION | RECONSTITUTION | EXPRESSION | AMPHIPOLS
Journal Article
NATURE COMMUNICATIONS, ISSN 2041-1723, 03/2019, Volume 10
Of the four separate PE biosynthetic pathways in eukaryotes, one occurs in the mitochondrial inner membrane (IM) and is executed by phosphatidylserine... 
CARDIOLIPIN | IMPORT | INTERMEMBRANE-SPACE | PROTEIN | MULTIDIMENSIONAL MASS-SPECTROMETRY | MULTIDISCIPLINARY SCIENCES | DETERGENT-FREE ISOLATION | C PEROXIDASE | IDENTIFICATION | SACCHAROMYCES-CEREVISIAE | POLYPEPTIDE
Journal Article
Journal Article
Journal of the American Chemical Society, ISSN 0002-7863, 12/2017, Volume 139, Issue 51, pp. 18657 - 18663
There is a growing interest in the use of lipid bilayer nanodiscs for various biochemical and biomedical applications. Among the different types of nanodiscs,... 
PHOSPHOLIPID-BILAYER NANODISCS | LIPODISQ | SOLID-STATE NMR | DETERGENT-FREE ISOLATION | ISLET AMYLOID POLYPEPTIDE | MEMBRANE-PROTEINS | FRAGMENTATION | RECONSTITUTION | CHEMISTRY, MULTIDISCIPLINARY | PEPTIDE | ACID COPOLYMER | Styrene | Chemical properties | Index Medicus
Journal Article
Chemical Communications, ISSN 1359-7345, 2018, Volume 54, Issue 69, pp. 9615 - 9618
Although there is a growing interest in using polymer lipid-nanodiscs, the polymer charge poses limitations for studies on membrane proteins. Here, we... 
NANOPARTICLES | PHOSPHOLIPID-BILAYER NANODISCS | SOLUBILIZATION | MALEIC ACID COPOLYMER | NMR-SPECTROSCOPY | DETERGENT-FREE ISOLATION | CYTOCHROME-P450 3A4 | CHEMISTRY, MULTIDISCIPLINARY | LIPID-BILAYER | INSIGHTS | Proteins | Cytochrome | Cytochromes | Polymers
Journal Article
Methods in Enzymology, ISSN 0076-6879, 2019, Volume 617, pp. 1 - 27
Numerous biosynthetic pathways have been shown to assemble at the surface of cellular membranes into efficient dynamic supramolecular assemblies termed... 
Styrene maleic acid copolymer | Detergent free | Microsome preparation | Styrene maleic acid lipid particles | Metabolon | Dhurrin metabolon | Metabolon isolation | Cytochromes P450 | NADPH-dependent cytochrome P450 oxidoreductase
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2017, Volume 7, Issue 1, pp. 11517 - 14
Styrene/maleic acid copolymers (SMA) have recently attracted great interest for in vitro studies of membrane proteins, as they self-insert into and fragment... 
SOLUBILIZATION | SURFACTANTS | MULTIDISCIPLINARY SCIENCES | PURIFICATION | DETERGENT-FREE ISOLATION | BILAYERS | MEMBRANE-PROTEINS | Differential scanning calorimetry | Spectroscopy | Nuclear magnetic resonance--NMR | Light scattering | Lipids | Phospholipids | pH effects | Membrane proteins | Magnetic resonance spectroscopy | Copolymers | Acids | Maleic acid | Styrene
Journal Article
Biophysical Journal, ISSN 0006-3495, 08/2018, Volume 115, Issue 3, pp. 494 - 502
Experimental characterization of membrane proteins often requires solubilization. A recent approach is to use styrene-maleic acid (SMA) copolymers to isolate... 
DYNAMICS SIMULATIONS | ALL-ATOM | BIOPHYSICS | BILAYER NANODISCS | COARSE-GRAINED MODEL | DETERGENT-FREE ISOLATION | FUNCTIONAL RECONSTITUTION | VERSATILE TOOL | FORCE-FIELD | PORE FORMATION | MEMBRANE-PROTEINS | Molecular dynamics | Thermodynamics | Membrane lipids | Membrane proteins | Index Medicus | Membranes
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2019, Volume 9, Issue 1, pp. 1813 - 10
Biological characterisation of membrane proteins lags behind that of soluble proteins. This reflects issues with the traditional use of detergents for... 
SOLUBILIZATION | BIOSYNTHESIS | MULTIDISCIPLINARY SCIENCES | ESCHERICHIA-COLI | Z-RING | DETERGENT-FREE ISOLATION | FUNCTIONAL RECONSTITUTION | INSIGHT | LIPID INTERACTIONS | BINDING | MASS-SPECTROMETRY | Proteins | Maleic acid | Styrene | Lipids | Bacteria | Phosphatidylglycerols | Phospholipids | Detergents | Membrane proteins | Structure-function relationships
Journal Article
Chemistry and Physics of Lipids, ISSN 0009-3084, 07/2019, Volume 221, pp. 30 - 38
Most membrane-solubilising amphiphilic copolymers such as diisobutylene/maleic acid (DIBMA) and styrene/maleic acid (SMA) carry high negative charge densities.... 
Coulombic repulsion | Membrane solubilization | Divalent cations | Polymer nanodiscs | Lipid exchange | SOLUBILIZATION | LIPID-BILAYER NANODISCS | BIOPHYSICS | BIOCHEMISTRY & MOLECULAR BIOLOGY | DETERGENT-FREE ISOLATION | MEMBRANE-PROTEINS
Journal Article
Structure, ISSN 0969-2126, 02/2017, Volume 25, Issue 2, pp. 384 - 392
Journal Article
Chemistry and Physics of Lipids, ISSN 0009-3084, 01/2019, Volume 218, pp. 85 - 90
Styrene-maleic acid (SMA) copolymers are increasingly gaining attention in the membrane protein field due to their ability to solubilize lipid membranes into... 
Native nanodiscs | Hydrolysis reaction | SMAnh | Autoclave | SMA | SMALPs | SOLUBILIZATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | DETERGENT-FREE ISOLATION | MODEL | NMR | BIOPHYSICS | MEMBRANE-PROTEINS | Hydrolysis | Methods | Maleic anhydride
Journal Article