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science & technology (23) 23
life sciences & biomedicine (22) 22
animals (18) 18
cho cells (17) 17
biotechnology & applied microbiology (16) 16
cricetinae (15) 15
cricetulus (14) 14
biotechnology (13) 13
cell culture techniques - methods (10) 10
humans (10) 10
biochemistry & molecular biology (9) 9
cell culture (8) 8
cho-dg44 (8) 8
dg44 (8) 8
biological and medical sciences (7) 7
fundamental and applied biological sciences. psychology (7) 7
dihydrofolate reductase (6) 6
gene expression (6) 6
proteins (6) 6
recombinant proteins (6) 6
recombinant proteins - genetics (6) 6
biochemistry, general (5) 5
cho (5) 5
life sciences (5) 5
recombinant proteins - biosynthesis (5) 5
suspension culture (5) 5
transfection (5) 5
analysis (4) 4
biotechnology - methods (4) 4
cho dg44 cells (4) 4
apoptosis (3) 3
biochemical research methods (3) 3
bioreactors (3) 3
cell biology (3) 3
cell growth (3) 3
cell lines (3) 3
cell survival (3) 3
cells, cultured (3) 3
cellular biology (3) 3
cho dg44 (3) 3
cho-dg44 cells (3) 3
dg44 cell (3) 3
dhfr (3) 3
mammalian cell culture (3) 3
mammalian cells (3) 3
microbiology (3) 3
protein expression (3) 3
research (3) 3
serum-free medium (3) 3
tetrahydrofolate dehydrogenase - deficiency (3) 3
tetrahydrofolate dehydrogenase - genetics (3) 3
transient gene expression (3) 3
zellwachstum (3) 3
animal cells (2) 2
antibody (2) 2
applied microbiology (2) 2
biochemistry (2) 2
bioreactor (2) 2
calcium (2) 2
cell line (2) 2
cell proliferation (2) 2
cell-growth (2) 2
cells (2) 2
chemistry (2) 2
cho cells - metabolism (2) 2
cho‐dg44 (2) 2
cho‐dg44 cells (2) 2
cloning, molecular (2) 2
comparative analysis (2) 2
culture media (2) 2
culture media - chemistry (2) 2
culture media, serum-free (2) 2
dg44 cells (2) 2
dg44细胞 (2) 2
electrophoresis, polyacrylamide gel (2) 2
fed-batch (2) 2
flp-in (2) 2
gene amplification (2) 2
genes (2) 2
genetic engineering (2) 2
genetic research (2) 2
genetic vectors (2) 2
genexpression (2) 2
growth (2) 2
hamsters (2) 2
hek293 cells (2) 2
lec 3.2.8.1 (2) 2
mammals (2) 2
methotrexate (2) 2
mice (2) 2
monoclonal antibodies (2) 2
optimization (2) 2
ovarian cancer (2) 2
packed cell volume (2) 2
q1 (2) 2
recombinant (2) 2
recombinant protein (2) 2
recombinant protein expression (2) 2
recombinant proteins - metabolism (2) 2
sera:from-blood-separated-fluids (2) 2
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1. Full Text Generation of Flp-in tm -ready DG44 and Lec 3.2.8.1 CHO cell lines for quick and easy constitutive protein expression
by Soler, D.C and Young, A.E and Vahedi-Faridi, A and McCormick, T.S
BioTechniques, ISSN 0736-6205, 07/2018, Volume 65, Issue 1, pp. 41 - 46
Flp-in | Lec 3.2.8.1 | Protein expression | CHO | DG44
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2. Full Text Generation of Flp-in
by Soler, DC and Young, AE and Vahedi-Faridi and McCormick, TS
BioTechniques, ISSN 0736-6205, 07/2018, Volume 65, Issue 1, pp. 41 - 46
Lec 3.2.8.1 | Flp-in | CHO | DG44 | protein expression
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3. Full Text Orbital shaker technology for the cultivation of mammalian cells in suspension
by Muller, Natalie and Girard, Philippe and Hacker, David L and Jordan, Martin and Wurm, Florian M
Biotechnology and bioengineering, ISSN 0006-3592, 02/2005, Volume 89, Issue 4, pp. 400 - 406
cultivation system | serum‐free medium | CHO‐DG44 cells | square bottles | orbital shaker | HEK‐293 EBNA cells | HEK-293 EBNA cells | CHO-DG44 cells | Serum-free medium | Cultivation system | Orbital shaker | Square bottles | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Fundamental and applied biological sciences. Psychology | Biotechnology | Establishment of new cell lines, improvement of cultural methods, mass cultures | Methods. Procedures. Technologies | Biological and medical sciences | Eukaryotic cell cultures | Animal cells | Biotechnology - methods | Gene Expression | Animals | Transfection | Cell Culture Techniques - economics | Humans | Cells, Cultured | Biotechnology - economics | Cell Culture Techniques - methods | Suspensions | Gene expression | Cell culture | Index Medicus | Q1 | Agitation | Precipitation | Suspension culture | Reporter gene | Calcium | Gene transfer | Mammalian cells
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4. Full Text Anti‐cell death engineering of CHO cells: Co‐overexpression of Bcl‐2 for apoptosis inhibition, Beclin‐1 for autophagy induction
by Lee, Jae Seong and Ha, Tae Kwang and Park, Jin Hyoung and Lee, Gyun Min
Biotechnology and bioengineering, ISSN 0006-3592, 08/2013, Volume 110, Issue 8, pp. 2195 - 2207
Bcl‐2 | apoptosis | autophagy | Beclin‐1 | CHO‐DG44 | Bcl-2 | Beclin-1 | Autophagy | CHO-DG44 | Apoptosis | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Apoptosis Regulatory Proteins - biosynthesis | Biotechnology - methods | Animals | Cricetulus | Cell Survival | Apoptosis Regulatory Proteins - genetics | Cell Culture Techniques - methods | Proto-Oncogene Proteins c-bcl-2 - biosynthesis | Proto-Oncogene Proteins c-bcl-2 - genetics | CHO Cells | Esters | Biochemistry | Ovarian cancer | Proteins | Genetic engineering | Index Medicus
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5. Full Text Enhanced cell culture performance using inducible anti‐apoptotic genes E1B‐19K and Aven in the production of a monoclonal antibody with Chinese hamster ovary cells
by Figueroa, Bruno and Ailor, Eric and Osborne, Douglas and Hardwick, J. Marie and Reff, Mitchell and Betenbaugh, Michael J
Biotechnology and bioengineering, ISSN 0006-3592, 07/2007, Volume 97, Issue 4, pp. 877 - 892
AVEN | fed‐batch | CHO‐DG44 | bioreactors mammalian cell culture | monoclonal antibody production | E1B‐19K | apoptosis | Chinese Hamster Ovary cells | Bcl‐2 family | caspases | glucose deprivation | Bioreactors mammalian cell culture | Glucose deprivation | Monoclonal antibody production | Caspases | Fed-batch | CHO-DG44 | E1B-19K | Apoptosis | Bcl-2 family | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Fundamental and applied biological sciences. Psychology | Biotechnology | Biological and medical sciences | Geum - genetics | Cricetinae | Cricetulus | Cell Survival - genetics | Apoptosis - genetics | Culture Media, Serum-Free | Cell Culture Techniques - methods | Antibodies, Monoclonal - genetics | Animals | Bioreactors | Models, Biological | Glucose - metabolism | Genetic Enhancement - methods | Adenovirus E1B Proteins - genetics | CHO Cells | Studies | Monoclonal antibodies | Cell culture | Mammals | Gene expression | Index Medicus
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6. Full Text Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells
by Hacker, David L and Kiseljak, Divor and Rajendra, Yashas and Thurnheer, Sarah and Baldi, Lucia and Wurm, Florian M
Protein expression and purification, ISSN 1046-5928, 11/2013, Volume 92, Issue 1, pp. 67 - 76
Transient gene expression | Polyethylenimine | Suspension culture | Transfection | HEK-293 | Recombinant protein | Orbital shaking | CHO-DG44 | Mammalian cells | Biochemistry & Molecular Biology | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Biochemical Research Methods | Science & Technology | Cricetinae | Drug Carriers - metabolism | Polyethyleneimine - metabolism | Viruses - genetics | Cell Culture Techniques - instrumentation | Cricetulus | Genetic Vectors - administration & dosage | Humans | Recombinant Proteins - genetics | Equipment Design | Animals | Transfection - methods | HEK293 Cells | CHO Cells | Gene expression | Genes | Cells | Index Medicus
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7. Full Text Optimized dual assay for the transgenes selection and screening in CHO cell line development for recombinant protein production
by Beketova, Elena V and Ibneeva, Liliia R and Abdulina, Yulia A and Dergousova, Elena A and Filatov, Vladimir L and Kozlovsky, Stanislav V and Shilov, Evgeny S and Datskevich, Petr N and Rozov, Fedor N
Biotechnology letters, ISSN 0141-5492, 9/2019, Volume 41, Issue 8, pp. 929 - 939
Life Sciences | Biochemistry, general | DHFR | Biotechnology | Microbiology | Selection | Applied Microbiology | CHO DG44 | Resazurin | Recombinant mAbs | Recombinant proteins | Optimization | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Measurement | Usage | Epithelial cells | Analysis | Biological assay | Comparative analysis | Production processes | Genetic screening | Methods | Dyes and dyeing | Proteins | Productivity | Heterogeneity | Screening | Immunoassay | Transfection | Growth rate | Cloning | Workflow | Protein expression | Transgenes | Deoxyribonucleic acid--DNA | Index Medicus
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8. Full Text Identifying the CHO Secretome using Mucin-type O‑Linked Glycosylation and Click-chemistry
by Slade, Peter G and Hajivandi, Mahbod and Bartel, Cheryl Moody and Gorfien, Stephen F
Journal of proteome research, ISSN 1535-3893, 12/2012, Volume 11, Issue 12, pp. 6175 - 6186
click-chemistry | CHO-S | host cell proteins | GalNAz | O-linked glycosylation | CHO | DG44 | secretome | Biochemistry & Molecular Biology | Life Sciences & Biomedicine | Biochemical Research Methods | Science & Technology | Cricetinae | Reproducibility of Results | Secretory Pathway | Click Chemistry - methods | Cytoplasm - metabolism | Nuclear Proteins - metabolism | Glycosylation | Proteome - analysis | Culture Media - metabolism | Galactosamine - analogs & derivatives | Polysaccharides - metabolism | Galactosamine - metabolism | Animals | Cell Nucleus - metabolism | Time Factors | Staining and Labeling | Mucins - metabolism | Proteomics - methods | Proteome - metabolism | CHO Cells | Index Medicus
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9. Full Text GLP-1-Fc融合蛋白在DG44细胞中的表达及其质量分析
by 骆海燕 and 谭婉珑 and 张亚楠 and 洪厚胜
南京工业大学学报（自然科学版）, ISSN 1671-7627, 2017, Volume 39, Issue 6, pp. 74 - 81
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10. Culture process for fully human anti-VEGF165 monoclonal antibody
by Li, Xinran and Liu, Rui and Liu, Fangkun and Liu, Yu
Journal of China Pharmaceutical University, ISSN 1000-5048, 12/2015, Volume 46, Issue 6, pp. 734 - 739
Fully human monoclonal antibody | Bioreactor | Process optimization | Suspension culture | DG44 cells
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11. Full Text Low‐temperature pausing of cultivated mammalian cells
by Hunt, Lisa and Hacker, David L and Grosjean, Frédéric and De Jesus, Maria and Uebersax, Lorenz and Jordan, Martin and Wurm, Florian M
Biotechnology and bioengineering, ISSN 0006-3592, 01/2005, Volume 89, Issue 2, pp. 157 - 163
HEK293E cells | cell storage | IgG antibody | CHO‐DG44 cells | hypothermia | green fluorescent protein | CHO-DG44 cells | Cell storage | Green fluorescent protein | Hypothermia | Life Sciences & Biomedicine | Biotechnology & Applied Microbiology | Science & Technology | Fundamental and applied biological sciences. Psychology | Biotechnology | Biological and medical sciences | Kidney - physiology | Cell Line | Cricetinae | Temperature | Cell Proliferation | Cricetulus | Humans | Cell Culture Techniques - methods | Cryopreservation - methods | Animals | Time Factors | Bioreactors | Cell Survival - physiology | CHO Cells | Mammals | Cells | Index Medicus | Q1 | Cell culture | Suspension culture | Calcium | Temperature effects | Embryos | Freezing | Mammalian cells
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12. Full Text High-level expression and preparation of recombinant human fibrinogen as biopharmaceuticals
by Hirashima, Masaki and Imamura, Takayuki and Yano, Kentaro and Kawamura, Ryoichi and Meta, Akihiro and Tokieda, Yoshiyuki and Nakashima, Toshihiro
Journal of biochemistry (Tokyo), ISSN 0021-924X, 06/2015, Volume 159, Issue 2, pp. 261 - 270
biopharmaceuticals | CHO DG44 | high-recovery preparation | high-level expression | recombinant human fibrinogen | Life Sciences & Biomedicine | Biochemistry & Molecular Biology | Science & Technology | Cricetinae | Microscopy, Electron, Scanning | Cricetulus | Electrophoresis, Polyacrylamide Gel | Humans | Recombinant Proteins - biosynthesis | Biological Products - isolation & purification | Blotting, Western | Recombinant Proteins - isolation & purification | Fibrinogen - isolation & purification | Animals | Fibrinogen - biosynthesis | Biological Products - metabolism | Thrombin - metabolism | Fibrin - metabolism | CHO Cells | Index Medicus | Regular Papers
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13. Full Text Design of a stable cell line producing a recombinant monoclonal anti-TNFα antibody based on a CHO cell line
by Voronina, E V and Seregin, Y A and Litvinova, N A and Shvets, V I and Shukurov, R R
SpringerPlus, ISSN 2193-1801, 12/2016, Volume 5, Issue 1, pp. 1 - 11
Monoclonal anti-TNFα antibody | Dihydrofolate reductase mediated gene amplification | Tumor necrosis factor alpha | Science, Humanities and Social Sciences, multidisciplinary | CHO-DG44 | Science & Technology - Other Topics | Multidisciplinary Sciences | Science & Technology
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