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index medicus (138) 138
differential scanning fluorometry (85) 85
biochemistry & molecular biology (74) 74
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differential scanning fluorimetry (38) 38
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differential scanning calorimetry (31) 31
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circular dichroism (15) 15
crystallography, x-ray (15) 15
protein folding (15) 15
amino acid sequence (14) 14
crystallization (14) 14
enzymes (14) 14
fluorimetry (14) 14
hot temperature (14) 14
nanoparticles (14) 14
protein conformation (14) 14
thermal shift assays (14) 14
affinity (13) 13
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calorimetry, differential scanning - methods (13) 13
medicine (13) 13
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proteins - chemistry (13) 13
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high-throughput screening assays - methods (12) 12
inhibitors (12) 12
research article (12) 12
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methods (11) 11
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research (11) 11
spectrometry, fluorescence (11) 11
thermal shift assay (11) 11
diagnosis, differential (10) 10
differential pulse voltammetry (10) 10
dna (10) 10
fluorescent dyes - chemistry (10) 10
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protein structure, secondary (10) 10
science (10) 10
structure-activity relationship (10) 10
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electrochemistry (9) 9
enzyme stability (9) 9
high-throughput screening assays (9) 9
hydrogen-ion concentration (9) 9
mice (9) 9
molecular biology (9) 9
protein unfolding (9) 9
thermal stability (9) 9
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food science & technology (8) 8
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magnetic resonance spectroscopy (8) 8
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1. Full Text Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization
by Simeonov, Anton
Expert Opinion on Drug Discovery, ISSN 1746-0441, 09/2013, Volume 8, Issue 9, pp. 1071 - 1082
Introduction: Despite tremendous advances in the application of biophysical methods in drug discovery, the preponderance of instruments and techniques still... 
chemical chaperones | pharmacological chaperones | inhibitor mechanism of action | fluorescence thermal shift | thermal stabilization | differential scanning fluorometry | Inhibitor mechanism of action | Thermal stabilization | Pharmacological chaperones | Chemical chaperones | Differential scanning fluorometry | Fluorescence thermal shift | LIGAND-BINDING | STABILITY | DRUG DISCOVERY | MONOCLONAL-ANTIBODY | IDENTIFICATION | THERMAL SHIFT ASSAYS | THERMODYNAMICS | FLUORESCENCE | AFFINITY | PHARMACOLOGY & PHARMACY | INHIBITORS | Fluorometry - methods | Proteins - chemistry | Drug Discovery | Humans
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2. Full Text High-Throughput Evaluation Method for Drug Association with Pregnane X Receptor (PXR) Using Differential Scanning Fluorometry
by Sekiguchi, Mitsuhiro and Kobashigawa, Yoshihiro and Moriguchi, Hiroyuki and Kawasaki, Masashi and Yuda, Masamichi and Teramura, Toshio and Inagaki, Fuyuhiko
Journal of Biomolecular Screening, ISSN 1087-0571, 10/2013, Volume 18, Issue 9, pp. 1084 - 1091
The pregnane xenobiotic receptor (PXR) is a key transcriptional regulator of cytochrome P450 (CYP) 3A, a crucial enzyme in the metabolism and detoxification of... 
differential scanning calorimeter | DSC | DSF | pregnane X receptor | PXR | reporter gene assay | differential scanning fluorometry | CHEMISTRY, ANALYTICAL | ACTIVATION | PROTEIN | BIOCHEMICAL RESEARCH METHODS | CYP3A4 INDUCTION | XENOBIOTIC RECEPTOR | THERMAL SHIFT ASSAYS | STRUCTURAL DETERMINANTS | DISCOVERY | HUMAN HEPATOCYTES | CAR | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | Receptors, Steroid - metabolism | Humans | Anticholesteremic Agents - chemistry | Fluorometry - methods | Nuclear Receptor Coactivator 1 - chemistry | Recombinant Fusion Proteins - chemistry | Recombinant Fusion Proteins - metabolism | Nuclear Receptor Coactivator 1 - metabolism | Receptors, Steroid - chemistry | Thermodynamics | Nuclear Receptor Coactivator 1 - genetics | High-Throughput Screening Assays | Cytochrome P-450 CYP3A - genetics | Receptors, Steroid - genetics | Cytochrome P-450 CYP3A - metabolism | Escherichia coli - genetics | Diphosphonates - chemistry | Cytochrome P-450 CYP3A - chemistry | Escherichia coli - metabolism | Protein Binding | Recombinant Fusion Proteins - genetics | Calorimetry, Differential Scanning | Kinetics | Fluorescent Dyes | Xenobiotics | Calorimetry | Analysis
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3. Full Text Selective protein unfolding: A universal mechanism of action for the development of irreversible inhibitors
by Askin, Samuel and Bond, Thomas E. H and Sorenson, Alanna E and Moreau, Morgane J. J and Antony, Helma and Davis, Rohan A and Schaeffer, Patrick M
Chemical Communications, ISSN 1359-7345, 2018, Volume 54, Issue 14, pp. 1738 - 1741
High-throughput differential scanning fluorimetry of GFP-tagged proteins (HT-DSF-GTP) was applied for the identification of novel enzyme inhibitors acting by a... 
CYTOCHROME-C | TARGET | LIGAND-BINDING | ASSAY | NUCLEOPROTEIN | DRUG DISCOVERY | ANTIBACTERIAL | DEGRADATION | DIFFERENTIAL SCANNING FLUOROMETRY | FUNCTIONAL-CHARACTERIZATION | CHEMISTRY, MULTIDISCIPLINARY
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4. Full Text Investigating the Impact of Polymer Functional Groups on the Stability and Activity of Lysozyme–Polymer Conjugates
by Lucius, Melissa and Falatach, Rebecca and McGlone, Cameron and Makaroff, Katherine and Danielson, Alex and Williams, Cameron and Nix, Jay C and Konkolewicz, Dominik and Page, Richard C and Berberich, Jason A
Biomacromolecules, ISSN 1525-7797, 03/2016, Volume 17, Issue 3, pp. 1123 - 1134
Polymers are often conjugated to proteins to improve stability; however, the impact of polymer chain length and functional groups on protein structure and... 
POLYMER SCIENCE | PROTEIN | ENZYME-ACTIVITY | STABILIZATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | CHEMISTRY, ORGANIC | GRAFTING-FROM | ATRP | SUBSTRATE | SURFACE | DIFFERENTIAL SCANNING FLUOROMETRY | RESPONSIVE POLYMERS | PEGYLATION | Muramidase - pharmacology | Cell Wall - drug effects | Muramidase - chemistry | Acrylic Resins - chemistry | Micrococcus - drug effects | Enzyme Stability
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5. Full Text Fluorescence-based investigations of RNA-small molecule interactions
by McGovern-Gooch, Kayleigh R and Baird, Nathan J
Methods, ISSN 1046-2023, 09/2019, Volume 167, pp. 54 - 65
Interrogating non-coding RNA structures and functions with small molecules is an area of rapidly increasing interest among biochemists and chemical biologists.... 
DISPLACEMENT ASSAY | LIGAND-BINDING | 2-AMINOPURINE FLUORESCENCE | LONG NONCODING RNAS | RIBOSWITCH APTAMER | SINGLE-MOLECULE | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | TRIPLE-HELIX | DIFFERENTIAL SCANNING FLUOROMETRY | RIBOSOMAL-RNA | INDUCED CONFORMATIONAL-CHANGES | Fluorescence | RNA | Analysis | Investigations
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6. Full Text Isothermal Analysis of ThermoFluor Data can readily provide Quantitative Binding Affinities
by Bai, Nan and Roder, Heinrich and Dickson, Alex and Karanicolas, John
Scientific Reports, ISSN 2045-2322, 12/2019, Volume 9, Issue 1, pp. 2650 - 15
Differential scanning fluorimetry (DSF), also known as ThermoFluor or Thermal Shift Assay, has become a commonly-used approach for detecting protein-ligand... 
LIGAND-BINDING | CIRCULAR-DICHROISM | THERMODYNAMICS | INHIBITION | PROTEIN-STABILITY | MULTIDISCIPLINARY SCIENCES | DENATURATION | DIFFERENTIAL SCANNING FLUOROMETRY | THERMAL SHIFT ASSAYS | DETECT | HEAT-CAPACITY | Proteins | Temperature effects | Protein folding | Isoforms | Data processing | Ligands | Maltose-binding protein | Maltose
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7. Full Text Determining the Protein Stability of Alzheimer’s Disease Protein, Amyloid Precursor Protein
by Delport, Alexandré and Hewer, Raymond
The Protein Journal, ISSN 1572-3887, 8/2019, Volume 38, Issue 4, pp. 419 - 424
Determining protein thermal stability is integral in biomedical research. Here, with the use of two thermal stability assays, we show the melting temperature... 
Biochemistry, general | Chemistry | Cellular thermal shift assay | Alzheimer’s disease | Differential scanning fluorometry | Animal Anatomy / Morphology / Histology | Bioorganic Chemistry | Amyloid precursor protein | Organic Chemistry | Proteins | Temperature | Profiling | Melting | Precursors | Melt temperature | Fluorometry | Fluorimetry | Alzheimer's disease | Thermal stability
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8. Full Text The ReFOLD assay for protein formulation studies and prediction of protein aggregation during long-term storage
by Svilenov, Hristo and Winter, Gerhard
European Journal of Pharmaceutics and Biopharmaceutics, ISSN 0939-6411, 04/2019, Volume 137, pp. 131 - 139
The formulation of novel therapeutic proteins is a challenging task which aims at finding formulation conditions that will minimize protein degradation during... 
Proteins | Protein aggregation | Protein folding | Storage stability | Protein unfolding | Monoclonal antibody | Protein formulation | Stability prediction | MECHANISM | ISOTHERMAL CHEMICAL DENATURATION | MONOCLONAL-ANTIBODY | IGG2 | DEPENDENCE | IMMUNOGENICITY | PHARMACOLOGY & PHARMACY | DIFFERENTIAL SCANNING FLUOROMETRY | CONFORMATIONAL STABILITY | TOOL | Monoclonal antibodies | Proteolysis | Denaturation
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9. Selective protein unfolding: a universal mechanism of action for the development of irreversible inhibitors
by Samuel Askin and Thomas E H Bond and Alanna E Sorenson and Morgane J J Moreau and Helma Antony and Rohan A Davis and Patrick M Schaeffer
Chemical Communications : Chem Comm, ISSN 1359-7345, 01/2018, Volume 54, Issue 14, pp. 1738 - 1741
High-throughput differential scanning fluorimetry of GFP-tagged proteins (HT-DSF-GTP) was applied for the identification of novel enzyme inhibitors acting by a... 
Proteins | Target recognition | Inhibitors | Workflow | Biotin | Fluorimetry | Biocides
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10. Full Text Comparative functional analysis of ribonuclease 1 homologs: Molecular insights into evolving vertebrate physiology
by Lomax, Jo E and Eller, Chelcie H and Raines, Ronald T
Biochemical Journal, ISSN 0264-6021, 07/2017, Volume 474, Issue 13, pp. 2219 - 2233
Pancreatic-type ribonucleases (ptRNases) comprise a class of highly conserved secretory endoribonucleases in vertebrates. The prototype of this enzyme family... 
RNASE | PROTEIN | EVOLUTION | GENE | HIGH-THROUGHPUT | ACTIVE-SITE | BIOCHEMISTRY & MOLECULAR BIOLOGY | HUMAN PANCREATIC RIBONUCLEASE | DIFFERENTIAL SCANNING FLUOROMETRY | INNATE IMMUNITY | A SUPERFAMILY | Amino Acid Sequence | Cats | Humans | Vertebrates - physiology | Phylogeny | Ribonucleases - metabolism | Sequence Homology, Amino Acid | Animals | Cattle | Chickens | Horses | Mice | Ranidae | Evolution, Molecular | RNA - metabolism
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11. Full Text Strategies for Biophysical Characterization of Protein–Polymer Conjugates
by Williams, Cameron and Dougherty, Melissa L and Makaroff, Katherine and Stapleton, Jacob and Konkolewicz, Dominik and Berberich, Jason A and Page, Richard C
Methods in Enzymology, ISSN 0076-6879, 2017, Volume 590, pp. 93 - 114
Protein–polymer conjugates are increasingly viewed as promising avenues to producing industrial enzymes with high activity capable of withstanding potentially... 
Thermodynamic parameters | Tryptophan fluorescence | Biophysical assay | Protein–polymer conjugate | Differential scanning fluorimetry | CIRCULAR-DICHROISM | NANOCONSTRUCTS | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | DIFFERENTIAL SCANNING FLUOROMETRY | DIVERSITY | STABILIZING PROTEINS | CONFORMATION | GUANIDINIUM CHLORIDE | RAFT POLYMERIZATION | GRAFTING-FROM | POLYPEPTIDE | Thermodynamics | Immobilized Proteins - chemistry | Spectrometry, Fluorescence | Tryptophan - chemistry | Calorimetry, Differential Scanning | Polymers - chemistry | Protein Stability | Proteins - chemistry
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12. Full Text Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription
by Bütepage, Mareike and Preisinger, Christian and Von Kriegsheim, Alexander and Scheufen, Anja and Lausberg, Eva and Li, Jinyu and Kappes, Ferdinand and Feederle, Regina and Ernst, Sabrina and Eckei, Laura and Krieg, Sarah and Müller-Newen, Gerhard and Rossetti, Giulia and Feijs, Karla L. H and Verheugd, Patricia and Lüscher, Bernhard
Scientific Reports, ISSN 2045-2322, 12/2018, Volume 8, Issue 1, pp. 1 - 17
Macrodomains are conserved protein folds associated with ADP-ribose binding and turnover. ADP-ribosylation is a posttranslational modification catalyzed... 
MACRODOMAINS | RNA-BINDING | PROTEOME-WIDE IDENTIFICATION | GENOTOXIC STRESS | POLY(ADP-RIBOSE) GLYCOHYDROLASE | MULTIDISCIPLINARY SCIENCES | SARS CORONAVIRUS | DIFFERENTIAL SCANNING FLUOROMETRY | TUMOR-CELLS | PROTEINS | MASS-SPECTROMETRY | Transcription factors | Transcription | rRNA | DNA damage | Ribosomes | Poly(ADP-ribose) polymerase | Nucleoli | Poly(ADP-ribose) Polymerase 1 | Ribose | Quality control | Ribosylation | RNA processing | Deoxyribonucleic acid--DNA | ADP-ribosylation
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13. Full Text A Glycosaminoglycan Extract from Portunus pelagicus Inhibits BACE1, the beta Secretase Implicated in Alzheimer's Disease
by Mycroft-West, CJ and Cooper, LC and Devlin, AJ and Procter, P and Guimond, SE and Guerrini, M and Fernig, DG and Lima, MA and Yates, EA and Skidmore, MA
MARINE DRUGS, ISSN 1660-3397, 05/2019, Volume 17, Issue 5
Therapeutic options for Alzheimer's disease, the most common form of dementia, are currently restricted to palliative treatments. The glycosaminoglycan... 
amyloid- beta | CHEMISTRY, MEDICINAL | Portunus pelagicus | MEMORY DEFICITS | FUCOSYLATED CHONDROITIN SULFATE | BACE1 | heparin | SHRIMP | CIRCULAR-DICHROISM | GLUCURONIC-ACID | beta-secretase | MOLECULAR-WEIGHT HEPARIN | MOUSE MODEL | heparan sulphate | DIFFERENTIAL SCANNING FLUOROMETRY | Alzheimer's disease | glycosaminoglycan | BINDING | BRAIN
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14. Full Text Substituted anthraquinones represent a potential scaffold for DNA methyltransferase 1-specific inhibitors
by Switzer, RL and Medrano, J and Reedel, DA and Weiss, J
PLOS ONE, ISSN 1932-6203, 07/2019, Volume 14, Issue 7, p. e0219830
In humans, the most common epigenetic DNA modification is methylation of the 5-carbon of cytosines, predominantly in CpG dinucleotides. DNA methylation is an... 
METHYLATION | DIFFERENTIAL SCANNING FLUOROMETRY | DNMT3A MUTATIONS | CANCER | MULTIDISCIPLINARY SCIENCES | ANTISENSE | Research | Methylation | Gene expression | Anthraquinones | Competition | Incorporation | Leukemia | Biochemistry | Biology | Selectivity | Substitutes | Anthraquinone | Aromatic compounds | Cell cycle | DNA methylation | Tumorigenesis | Deoxyribonucleic acid--DNA | CpG islands | Enzymes | DNMT1 protein | Breast cancer | Mammals | Inhibitors | Natural products | Epigenetics | DNA methyltransferase | Mutation | Disruption | Endonuclease | Scaffolds | Deoxyribonucleic acid | DNA
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15. Full Text Food-grade Pickering stabilizers obtained from a protein-rich lupin cultivar (AluProt-CGNA®): Chemical characterization and emulsifying properties
by Burgos-Díaz, César and Wandersleben, Traudy and Olivos, Marcos and Lichtin, Nicole and Bustamante, Mariela and Solans, Conxita
Food Hydrocolloids, ISSN 0268-005X, 02/2019, Volume 87, pp. 847 - 857
Pickering stabilizers exhibit unique interfacial properties; however, only limited types of these particles are available for the food industry. Therefore, the... 
Protein-rich | Pickering emulsions | AluProt-CGNA | Lupin protein isolate | Pickering stabilizers | Emulsifying properties | SOY PROTEIN | PARTICLES | FOOD SCIENCE & TECHNOLOGY | HEAT-DENATURED WHEY | SALT ADDITION | AluProt-CGNA (R) | ALUPROT-CGNA | POTENTIAL APPLICATIONS | HIGH-PRESSURE HOMOGENIZATION | DIFFERENTIAL SCANNING FLUOROMETRY | CHEMISTRY, APPLIED | IONIC-STRENGTH | IN-WATER EMULSIONS | Proteins | Biotechnology | Genetically engineered foods | Food
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16. Full Text Screening and Evaluation of Small Organic Molecules as CIpB Inhibitors and Potential Antimicrobials
by Martin, I and Underhaug, J and Celaya, G and Moro, F and Teigen, K and Martinez, A and Muga, A
JOURNAL OF MEDICINAL CHEMISTRY, ISSN 0022-2623, 09/2013, Volume 56, Issue 18, pp. 7177 - 7189
Inhibition of CIpB, the bacterial representative of the heat-shock protein 100 family that is associated with virulence of several pathogens, could be an... 
CHEMISTRY, MEDICINAL | ASSAY | DNAK | HIGH-THROUGHPUT SCREEN | ESCHERICHIA-COLI | DRUG DISCOVERY | DIFFERENTIAL SCANNING FLUOROMETRY | STRESS TOLERANCE | CHAPERONE | SHOCK-PROTEIN CLPB | AGGREGATION
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17. Full Text A methodological approach for the thermal stability and stress exposure studies of a model antibody
by Coussot, Gaëlle and Faye, Clément and Le Postollec, Aurélie and Dobrijevic, Michel
Analytical Biochemistry, ISSN 0003-2697, 05/2018, Volume 548, pp. 23 - 31
The anti-horseradish peroxidase (HRP) antibody is conventionally used in immunohistochemistry. More recently, it has been used as the key element in a gold... 
Stress exposures | Horseradish peroxidase | A2HRP method | Antibody stability | Freeze-drying | Quality control | CHEMISTRY, ANALYTICAL | ASSAY | STABILIZATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | MONOCLONAL-ANTIBODY | CRITICAL REAGENTS | PEROXIDASE | Horseradish percoddase | RECOMMENDATIONS | FUNCTIONALITY | DIFFERENTIAL SCANNING FLUOROMETRY | PROTEIN FORMULATIONS | MELT CURVES | Immunohistochemistry | Viral antibodies | Antibodies | Peroxidase | Analysis
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