FEMS Microbiology Ecology, ISSN 0168-6496, 12/2012, Volume 82, Issue 3, pp. 666 - 677
Abstract With recent methodological advances, molecular markers are increasingly used for semi-quantitative analyses of fungal communities. The aim to preserve...
barcoding | diversity | PCR bias | fungal communities | Fungal communities | Barcoding | Diversity | BIAS | DNA BARCODE | ECOLOGY | MICROBIOLOGY | PCR | DNA, Fungal - analysis | DNA, Fungal - genetics | DNA, Ribosomal Spacer | Fungi - classification | Fungi - isolation & purification | DNA Primers - genetics | Fungi - genetics | Mycological Typing Techniques | Genes | Genetics | Microbiology | Skogsvetenskap | Agricultural Science | Mikrobiologi | Ecology | Jordbruksvetenskap | Forest Science | Ekologi
barcoding | diversity | PCR bias | fungal communities | Fungal communities | Barcoding | Diversity | BIAS | DNA BARCODE | ECOLOGY | MICROBIOLOGY | PCR | DNA, Fungal - analysis | DNA, Fungal - genetics | DNA, Ribosomal Spacer | Fungi - classification | Fungi - isolation & purification | DNA Primers - genetics | Fungi - genetics | Mycological Typing Techniques | Genes | Genetics | Microbiology | Skogsvetenskap | Agricultural Science | Mikrobiologi | Ecology | Jordbruksvetenskap | Forest Science | Ekologi
Journal Article
BMC bioinformatics, ISSN 1471-2105, 2012, Volume 13, Issue 1, pp. 134 - 134
Background: Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of...
QUANTITATIVE PCR | SEQUENCES | SEARCH | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | BIOCHEMICAL RESEARCH METHODS | MATHEMATICAL & COMPUTATIONAL BIOLOGY | Carrier Proteins - genetics | Algorithms | Introns | Humans | Molecular Sequence Data | DNA Primers - genetics | Polymorphism, Single Nucleotide | Software | Polymerase Chain Reaction - methods | Polymerase chain reaction | Explosions | Single nucleotide polymorphisms | Genetic algorithms | Design | Biomedical research | C plus plus | Deoxyribonucleic acid--DNA | Programming languages | Data bases | Databases | Nucleotide sequence | software | Exons | Primers | Single-nucleotide polymorphism | Bioinformatics | Computer programs
QUANTITATIVE PCR | SEQUENCES | SEARCH | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | BIOCHEMICAL RESEARCH METHODS | MATHEMATICAL & COMPUTATIONAL BIOLOGY | Carrier Proteins - genetics | Algorithms | Introns | Humans | Molecular Sequence Data | DNA Primers - genetics | Polymorphism, Single Nucleotide | Software | Polymerase Chain Reaction - methods | Polymerase chain reaction | Explosions | Single nucleotide polymorphisms | Genetic algorithms | Design | Biomedical research | C plus plus | Deoxyribonucleic acid--DNA | Programming languages | Data bases | Databases | Nucleotide sequence | software | Exons | Primers | Single-nucleotide polymorphism | Bioinformatics | Computer programs
Journal Article
Plant Pathology, ISSN 0032-0862, 06/2010, Volume 59, Issue 3, pp. 453 - 464
Several published polymerase chain reaction (PCR) primers to identify Pseudomonas syringae pv. actinidiae, the causal organism of bacterial canker of...
multilocus sequence analysis | biosecurity | quarantine | Actinidia spp | bacterial canker | argK gene | Quarantine | Biosecurity | ArgK gene | Bacterial canker | Multilocus sequence analysis | AGRONOMY | NEW-ZEALAND | ERWINIA-AMYLOVORA | CANKER | BLIGHT | ARGK | IDENTIFICATION | PHASEOLICOLA | PLANT SCIENCES | SYRINGOMYCIN | RELATEDNESS | KIWIFRUIT | Polymerase chain reaction | Genetic research | Analysis | DNA | Fertilizers
multilocus sequence analysis | biosecurity | quarantine | Actinidia spp | bacterial canker | argK gene | Quarantine | Biosecurity | ArgK gene | Bacterial canker | Multilocus sequence analysis | AGRONOMY | NEW-ZEALAND | ERWINIA-AMYLOVORA | CANKER | BLIGHT | ARGK | IDENTIFICATION | PHASEOLICOLA | PLANT SCIENCES | SYRINGOMYCIN | RELATEDNESS | KIWIFRUIT | Polymerase chain reaction | Genetic research | Analysis | DNA | Fertilizers
Journal Article
植物学报:英文版, ISSN 1672-9072, 2015, Volume 57, Issue 7, pp. 613 - 617
The clustered regularly interspaced short palin- dromic repeats (CRiSPR)-associated system enables biologists to edit genomes precisely and provides a powerful...
引物设计 | 成像系统 | 染色体 | Cas系统 | 基因组结构 | 多参数分析 | 基因敲除 | 设计工具 | knockout | CRISPR | software | primer | Chromosome imaging | Primer | Software | Knockout | RNA | HUMAN-CELLS | BIOCHEMISTRY & MOLECULAR BIOLOGY | MODEL | NUCLEASES | PLANT SCIENCES | ENDONUCLEASE | PLANTS | TARGETED MUTAGENESIS | MULTIPLEX | TOOL | DNA Primers - metabolism | Clustered Regularly Interspaced Short Palindromic Repeats - genetics | Chromosomes, Plant - genetics | Gene Knockout Techniques | Imaging, Three-Dimensional | CRISPR-Cas Systems - genetics
引物设计 | 成像系统 | 染色体 | Cas系统 | 基因组结构 | 多参数分析 | 基因敲除 | 设计工具 | knockout | CRISPR | software | primer | Chromosome imaging | Primer | Software | Knockout | RNA | HUMAN-CELLS | BIOCHEMISTRY & MOLECULAR BIOLOGY | MODEL | NUCLEASES | PLANT SCIENCES | ENDONUCLEASE | PLANTS | TARGETED MUTAGENESIS | MULTIPLEX | TOOL | DNA Primers - metabolism | Clustered Regularly Interspaced Short Palindromic Repeats - genetics | Chromosomes, Plant - genetics | Gene Knockout Techniques | Imaging, Three-Dimensional | CRISPR-Cas Systems - genetics
Journal Article
PLoS Computational Biology, ISSN 1553-734X, 02/2010, Volume 6, Issue 2, p. e1000667
Metagenomics is a discipline that enables the genomic study of uncultured microorganisms. Faster, cheaper sequencing technologies and the ability to sequence...
DNA-SEQUENCES | DATA-MANAGEMENT | FUNCTIONAL DIVERSITY | MICROBIAL DIVERSITY | VIRAL COMMUNITIES | MINIMUM INFORMATION | BIOCHEMICAL RESEARCH METHODS | MATHEMATICAL & COMPUTATIONAL BIOLOGY | PHYLOGENETIC CLASSIFICATION | MARINE ECOSYSTEMS | NUCLEOTIDE-SEQUENCE | 16S RIBOSOMAL-RNA | Metagenomics | Sequence Analysis, DNA | Computational Biology | Microbial genetics | Genomes | Research | Nucleotide sequencing | Identification and classification | Methods | Proteins | Earth | Microbiology | Genomics | Deoxyribonucleic acid | DNA | Cloning | Genetics
DNA-SEQUENCES | DATA-MANAGEMENT | FUNCTIONAL DIVERSITY | MICROBIAL DIVERSITY | VIRAL COMMUNITIES | MINIMUM INFORMATION | BIOCHEMICAL RESEARCH METHODS | MATHEMATICAL & COMPUTATIONAL BIOLOGY | PHYLOGENETIC CLASSIFICATION | MARINE ECOSYSTEMS | NUCLEOTIDE-SEQUENCE | 16S RIBOSOMAL-RNA | Metagenomics | Sequence Analysis, DNA | Computational Biology | Microbial genetics | Genomes | Research | Nucleotide sequencing | Identification and classification | Methods | Proteins | Earth | Microbiology | Genomics | Deoxyribonucleic acid | DNA | Cloning | Genetics
Journal Article
Nucleic Acids Research, ISSN 0305-1048, 01/2013, Volume 41, Issue 1, pp. e1 - e1
16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The...
ARB | OCEAN SAMPLING EXPEDITION | DESIGN | COMMUNITY ANALYSIS | DATABASE | BACTERIAL DIVERSITY | BIOCHEMISTRY & MOLECULAR BIOLOGY | DEEP-SEA | MICROBIOME | ENVIRONMENT | ONLINE RESOURCE | Genetic Variation | Computer Simulation | Polymerase Chain Reaction | RNA, Ribosomal, 16S - genetics | Archaea - genetics | Genes, rRNA | High-Throughput Nucleotide Sequencing | Biodiversity | DNA Primers | Bacteria - genetics | Metagenome | Sequence Analysis, DNA | Methods Online
ARB | OCEAN SAMPLING EXPEDITION | DESIGN | COMMUNITY ANALYSIS | DATABASE | BACTERIAL DIVERSITY | BIOCHEMISTRY & MOLECULAR BIOLOGY | DEEP-SEA | MICROBIOME | ENVIRONMENT | ONLINE RESOURCE | Genetic Variation | Computer Simulation | Polymerase Chain Reaction | RNA, Ribosomal, 16S - genetics | Archaea - genetics | Genes, rRNA | High-Throughput Nucleotide Sequencing | Biodiversity | DNA Primers | Bacteria - genetics | Metagenome | Sequence Analysis, DNA | Methods Online
Journal Article
The New England Journal of Medicine, ISSN 0028-4793, 05/2010, Volume 362, Issue 21, pp. 2001 - 2011
The practice of medicine is increasingly informed by genomic discovery. This review article describes the fundamental bases of genetic and genomic discovery...
Genome-Wide Association Study | Molecular Biology | Oligonucleotide Array Sequence Analysis | Epigenesis, Genetic | Genomics | Humans | Polymorphism, Single Nucleotide | Genetic Testing - methods | Sequence Analysis, DNA - methods
Genome-Wide Association Study | Molecular Biology | Oligonucleotide Array Sequence Analysis | Epigenesis, Genetic | Genomics | Humans | Polymorphism, Single Nucleotide | Genetic Testing - methods | Sequence Analysis, DNA - methods
Journal Article
2003, 2nd., ISBN 9780879696542, xi, 520
Book
Clinica Chimica Acta, ISSN 0009-8981, 06/2013, Volume 421, pp. 208 - 212
PCR-based technology is indispensable for genetic diagnosis. On the other hand, allele dropout is one significant cause of genotyping errors. Most allele...
Allele dropout | Non-primer-site SNV | Apparent homozygosity | Next generation primer design algorithm | FAH gene | Hairpin formation | DIAGNOSIS | ASSAY | POLYMORPHISM | IDENTIFICATION | GENE | MUTATIONS | MEDICAL LABORATORY TECHNOLOGY | Hydrolases - genetics | Humans | Genotyping Techniques - standards | Molecular Sequence Data | Genotype | Tyrosinemias - genetics | DNA Primers | Tyrosinemias - diagnosis | Artifacts | Homozygote | Algorithms | Pedigree | Alleles | Polymerase Chain Reaction - standards | Polymorphism, Single Nucleotide | Software | Nucleic Acid Conformation | Inverted Repeat Sequences
Allele dropout | Non-primer-site SNV | Apparent homozygosity | Next generation primer design algorithm | FAH gene | Hairpin formation | DIAGNOSIS | ASSAY | POLYMORPHISM | IDENTIFICATION | GENE | MUTATIONS | MEDICAL LABORATORY TECHNOLOGY | Hydrolases - genetics | Humans | Genotyping Techniques - standards | Molecular Sequence Data | Genotype | Tyrosinemias - genetics | DNA Primers | Tyrosinemias - diagnosis | Artifacts | Homozygote | Algorithms | Pedigree | Alleles | Polymerase Chain Reaction - standards | Polymorphism, Single Nucleotide | Software | Nucleic Acid Conformation | Inverted Repeat Sequences
Journal Article
2000, ISBN 9780415300070, x, 263
Book
1995, ISBN 9780879694470, viii, 714
Book
Nature Protocols, ISSN 1754-2189, 11/2015, Volume 10, Issue 11, pp. 1643 - 1669
Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPEAPEAPE) chemistries exploit small electrophilic reagents that react with 2'-hydroxyl groups...
REAGENT | BIOCHEMICAL RESEARCH METHODS | ARCHITECTURE | SINGLE-NUCLEOTIDE RESOLUTION | SECONDARY STRUCTURE | PARTITION-FUNCTION | REVEALS | Computational Biology - methods | RNA Processing, Post-Transcriptional | Models, Molecular | Mutation | Nucleic Acid Conformation | RNA - chemistry | Molecular Biology - methods | Acylation | Hydroxides | RNA | Gene mutations | Analysis | DNA polymerases | Influence | Research | Methods | motif discovery | mutational profiling | structure modeling | chemical probing
REAGENT | BIOCHEMICAL RESEARCH METHODS | ARCHITECTURE | SINGLE-NUCLEOTIDE RESOLUTION | SECONDARY STRUCTURE | PARTITION-FUNCTION | REVEALS | Computational Biology - methods | RNA Processing, Post-Transcriptional | Models, Molecular | Mutation | Nucleic Acid Conformation | RNA - chemistry | Molecular Biology - methods | Acylation | Hydroxides | RNA | Gene mutations | Analysis | DNA polymerases | Influence | Research | Methods | motif discovery | mutational profiling | structure modeling | chemical probing
Journal Article