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detergent-free isolation (48) 48
biochemistry & molecular biology (33) 33
index medicus (33) 33
membrane proteins (31) 31
solubilization (25) 25
lipids (24) 24
proteins (22) 22
membrane-proteins (19) 19
biophysics (18) 18
reconstitution (18) 18
protein (17) 17
detergent-free (16) 16
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styrene (15) 15
detergents (14) 14
maleic acid (13) 13
membranes (13) 13
nanoparticles (13) 13
chemistry, multidisciplinary (12) 12
maleates - chemistry (12) 12
membrane lipids (12) 12
analysis (11) 11
animals (11) 11
detergents, synthetic (11) 11
lipid bilayers - chemistry (11) 11
polymers (11) 11
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membrane proteins - chemistry (10) 10
nanodiscs (10) 10
cholesterol (9) 9
copolymer (9) 9
humans (9) 9
phospholipid-bilayer nanodiscs (9) 9
article (8) 8
detergens (8) 8
lipide (8) 8
maleic-acid (8) 8
maleinsäure (8) 8
membran (8) 8
model (8) 8
polystyrenes - chemistry (8) 8
protein conformation (8) 8
sma (8) 8
smalp (8) 8
solubility (8) 8
styrol (8) 8
animal and vegetable oils, fats, fatty substances and waxes (7) 7
apparatus using free vortex flow, e.g. cyclones (7) 7
bilayers (7) 7
binding (7) 7
candles (7) 7
cell biology (7) 7
centrifugal apparatus or machines for carrying-out physical orchemical processes (7) 7
chemistry (7) 7
copolymers (7) 7
doppelschicht (7) 7
essential oils (7) 7
fatty acids therefrom (7) 7
lipodisq (7) 7
metallurgy (7) 7
performing operations (7) 7
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phospholipids (7) 7
polymer (7) 7
producing, refining and preservingfats, fatty substances (7) 7
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caveolae (6) 6
detergent-free method (6) 6
detergents - chemistry (6) 6
membrane protein (6) 6
membrane proteins - metabolism (6) 6
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smalps (6) 6
styrenes (6) 6
usage (6) 6
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biochemical research methods (5) 5
cell membrane - metabolism (5) 5
free purification (5) 5
lipid bilayer (5) 5
lipid bilayers - metabolism (5) 5
lipid rafts (5) 5
multidisciplinary sciences (5) 5
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solid-state nmr (5) 5
styrene maleic acid (5) 5
amphipols (4) 4
bilayer nanodiscs (4) 4
channel (4) 4
detergent (4) 4
electron microscopy (4) 4
escherichia coli - metabolism (4) 4
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functional reconstitution (4) 4
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Acta biomaterialia, ISSN 1742-7061, 9/2018, Volume 77, pp. 116 - 126
Preservation of tissue structure is often a primary goal when optimizing tissue and organ decellularization methods. Many current protocols nonetheless rely on... 
Decellularization | peripheral nerve graft | apoptosis | detergent-free
Journal Article
Chemical Communications, ISSN 1359-7345, 2017, Volume 53, Issue 78, pp. 10824 - 10826
Polymer lipid nanodiscs have enabled some exciting structural biology and nanobiotechnology applications. The use of a small molecular weight polymer (SMA-EA)... 
NANOPARTICLES | NMR | SOLUBILIZATION | SIZE | DETERGENT-FREE ISOLATION | MODEL | MEMBRANE-PROTEINS | RECONSTITUTION | CHEMISTRY, MULTIDISCIPLINARY
Journal Article
CURRENT OPINION IN BIOTECHNOLOGY, ISSN 0958-1669, 08/2017, Volume 46, pp. 51 - 56
A plethora of polymer-based scaffolds have been designed to facilitate biochemical and biophysical investigation of membrane proteins, with a common goal to... 
CHANNEL | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | SMALPS | BIOCHEMICAL RESEARCH METHODS | DETERGENT-FREE ISOLATION | RECONSTITUTION | EXPRESSION | AMPHIPOLS
Journal Article
Biochemical Society Transactions, ISSN 0300-5127, 08/2016, Volume 44, Issue 4, pp. 1011 - 1018
Journal Article
NATURE COMMUNICATIONS, ISSN 2041-1723, 03/2019, Volume 10
Of the four separate PE biosynthetic pathways in eukaryotes, one occurs in the mitochondrial inner membrane (IM) and is executed by phosphatidylserine... 
CARDIOLIPIN | IMPORT | INTERMEMBRANE-SPACE | PROTEIN | MULTIDIMENSIONAL MASS-SPECTROMETRY | MULTIDISCIPLINARY SCIENCES | DETERGENT-FREE ISOLATION | C PEROXIDASE | IDENTIFICATION | SACCHAROMYCES-CEREVISIAE | POLYPEPTIDE
Journal Article
Journal of the American Chemical Society, ISSN 0002-7863, 12/2017, Volume 139, Issue 51, pp. 18657 - 18663
There is a growing interest in the use of lipid bilayer nanodiscs for various biochemical and biomedical applications. Among the different types of nanodiscs,... 
PHOSPHOLIPID-BILAYER NANODISCS | LIPODISQ | SOLID-STATE NMR | DETERGENT-FREE ISOLATION | ISLET AMYLOID POLYPEPTIDE | MEMBRANE-PROTEINS | FRAGMENTATION | RECONSTITUTION | CHEMISTRY, MULTIDISCIPLINARY | PEPTIDE | ACID COPOLYMER | Styrene | Chemical properties | Index Medicus
Journal Article
Chemical Communications, ISSN 1359-7345, 2018, Volume 54, Issue 69, pp. 9615 - 9618
Although there is a growing interest in using polymer lipid-nanodiscs, the polymer charge poses limitations for studies on membrane proteins. Here, we... 
NANOPARTICLES | PHOSPHOLIPID-BILAYER NANODISCS | SOLUBILIZATION | MALEIC ACID COPOLYMER | NMR-SPECTROSCOPY | DETERGENT-FREE ISOLATION | CYTOCHROME-P450 3A4 | CHEMISTRY, MULTIDISCIPLINARY | LIPID-BILAYER | INSIGHTS | Proteins | Cytochrome | Cytochromes | Polymers
Journal Article
International Journal of Biomedicine, ISSN 2158-0510, 06/2019, Volume 9, Issue Suppl_1, pp. S21 - S21
Background: The membrane-embedded 2:2 sensory rhodopsin II/transducer complex, NpSRII/NpHtrII, plays a key role in the photophobic response of the halophilic... 
detergent-free reconstitution | photoreceptor/transducer complex | nanolipoprotein particles
Journal Article
International Journal of Biomedicine, ISSN 2158-0510, 06/2019, Volume 9, Issue Suppl_1, pp. S18 - S18
Background: Now there are several approaches to isolate membrane proteins without detergents. Such approaches are promising as they guarantee the preservation... 
detergent-free solubilization methods | ion channels | membrane proteins
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2017, Volume 7, Issue 1, pp. 11517 - 14
Styrene/maleic acid copolymers (SMA) have recently attracted great interest for in vitro studies of membrane proteins, as they self-insert into and fragment... 
SOLUBILIZATION | SURFACTANTS | MULTIDISCIPLINARY SCIENCES | PURIFICATION | DETERGENT-FREE ISOLATION | BILAYERS | MEMBRANE-PROTEINS | Differential scanning calorimetry | Spectroscopy | Nuclear magnetic resonance--NMR | Light scattering | Lipids | Phospholipids | pH effects | Membrane proteins | Magnetic resonance spectroscopy | Copolymers | Acids | Maleic acid | Styrene
Journal Article
Methods in Enzymology, ISSN 0076-6879, 2019, Volume 617, pp. 1 - 27
Numerous biosynthetic pathways have been shown to assemble at the surface of cellular membranes into efficient dynamic supramolecular assemblies termed... 
Styrene maleic acid copolymer | Detergent free | Microsome preparation | Styrene maleic acid lipid particles | Metabolon | Dhurrin metabolon | Metabolon isolation | Cytochromes P450 | NADPH-dependent cytochrome P450 oxidoreductase
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2019, Volume 9, Issue 1, pp. 1813 - 10
Biological characterisation of membrane proteins lags behind that of soluble proteins. This reflects issues with the traditional use of detergents for... 
SOLUBILIZATION | BIOSYNTHESIS | MULTIDISCIPLINARY SCIENCES | ESCHERICHIA-COLI | Z-RING | DETERGENT-FREE ISOLATION | FUNCTIONAL RECONSTITUTION | INSIGHT | LIPID INTERACTIONS | BINDING | MASS-SPECTROMETRY | Proteins | Maleic acid | Styrene | Lipids | Bacteria | Phosphatidylglycerols | Phospholipids | Detergents | Membrane proteins | Structure-function relationships
Journal Article
Chemistry and Physics of Lipids, ISSN 0009-3084, 07/2019, Volume 221, pp. 30 - 38
Most membrane-solubilising amphiphilic copolymers such as diisobutylene/maleic acid (DIBMA) and styrene/maleic acid (SMA) carry high negative charge densities.... 
Coulombic repulsion | Membrane solubilization | Divalent cations | Polymer nanodiscs | Lipid exchange | SOLUBILIZATION | LIPID-BILAYER NANODISCS | BIOPHYSICS | BIOCHEMISTRY & MOLECULAR BIOLOGY | DETERGENT-FREE ISOLATION | MEMBRANE-PROTEINS
Journal Article
Structure, ISSN 0969-2126, 02/2017, Volume 25, Issue 2, pp. 384 - 392
Journal Article
Chemistry and Physics of Lipids, ISSN 0009-3084, 01/2019, Volume 218, pp. 85 - 90
Styrene-maleic acid (SMA) copolymers are increasingly gaining attention in the membrane protein field due to their ability to solubilize lipid membranes into... 
Native nanodiscs | Hydrolysis reaction | SMAnh | Autoclave | SMA | SMALPs | SOLUBILIZATION | BIOCHEMISTRY & MOLECULAR BIOLOGY | DETERGENT-FREE ISOLATION | MODEL | NMR | BIOPHYSICS | MEMBRANE-PROTEINS | Hydrolysis | Methods | Maleic anhydride
Journal Article
Journal Article
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, ISSN 0005-2728, 03/2018, Volume 1859, Issue 3, pp. 215 - 225
Intracytoplasmic vesicles (chromatophores) in the photosynthetic bacterium Rhodobacter sphaeroides represent a minimal structural and functional unit for... 
SMA | BIOCHEMISTRY & MOLECULAR BIOLOGY | DETERGENT-FREE ISOLATION | Synechocystis | CORE COMPLEXES | ORGANIZATION | Cytochrome bf | REACTION CENTERS | BIOPHYSICS | PHOTOSYNTHETIC ELECTRON-TRANSPORT | PURIFICATION | CHARGE SEPARATION | ENERGY-TRANSFER | Rba. sphaeroides | Cytochrome bc | Quinone pool | PHOTOSYSTEM-I | MEMBRANE-PROTEINS
Journal Article
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