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Applied Microbiology and Biotechnology, ISSN 0175-7598, 4/2019, Volume 103, Issue 8, pp. 3393 - 3406
Production of insulin-like growth factor 1 (IGF1) in Escherichia coli mostly results in the formation of inclusion bodies. In the present study, IGF1 was fused... 
Life Sciences | Biotechnology | Microbiology | Protein purification | Microbial Genetics and Genomics | Soluble expression | Box-Behnken design | Insulin-like growth factor 1 | HPLC | E. coli | INSULIN-LIKE-GROWTH-FACTOR-1 | E | coli | ESCHERICHIA-COLI | PARAMETERS | RECOMBINANT PROTEIN EXPRESSION | E. COLI | STRATEGIES | OVEREXPRESSION | AFFINITY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | FUSION PROTEIN | GROWTH-FACTOR-I | NIH 3T3 Cells | Molecular Weight | Insulin-Like Growth Factor I - chemistry | Protein Disulfide-Isomerases - metabolism | Humans | Insulin-Like Growth Factor I - genetics | Chromatography, High Pressure Liquid | Insulin-Like Growth Factor I - isolation & purification | Viral Proteins - metabolism | Recombinant Proteins - isolation & purification | Escherichia coli K12 - metabolism | Cysteine Endopeptidases - metabolism | Industrial Microbiology - methods | Models, Theoretical | Recombinant Proteins - metabolism | Gene Expression | Protein Structure, Secondary | Recombinant Proteins - chemistry | Escherichia coli Proteins - metabolism | Recombinant Proteins - genetics | Protein Disulfide-Isomerases - genetics | Animals | Chromatography, Affinity | Analysis of Variance | Escherichia coli Proteins - genetics | Cell Proliferation - drug effects | Mice | Escherichia coli K12 - genetics | Insulin-Like Growth Factor I - metabolism | Physiological aspects | Protein research | Research | Escherichia coli | Molecular structure | Insulin-like growth factor I | Oxidoreductase | Purity | Response surface methodology | Optimization | Molecular weight | Design optimization | Proteins | E coli | Imidazole | Inclusion bodies | Growth factors | Quality management | Purification | Liquid chromatography | Secondary structure | Sodium chloride | High-performance liquid chromatography | Insulin | Variance analysis | Biological activity | Weight | Protein expression | Yield | Protein structure
Journal Article
PLoS ONE, ISSN 1932-6203, 05/2017, Volume 12, Issue 5, p. e0176314
Journal Article
Gut, ISSN 0017-5749, 01/2016, Volume 65, Issue 1, pp. 33 - 46
Journal Article
2003, Methods in molecular biology, ISBN 1588290085, Volume 205., xi, 347 p.; ill.
Book
Journal Article
Science, ISSN 0036-8075, 4/2009, Volume 324, Issue 5924, pp. 255 - 258
Synonymous mutations do not alter the encoded protein, but they can influence gene expression. To investigate how, we engineered a synthetic library of 154... 
Messenger RNA | Genetic variation | Escherichia coli | Fluorescence | Reports | Libraries | Nucleotides | Codons | Gene expression |