Articles

Objective: To study the effects of vascular endothelial growth factor C small interfering RNA and endostatin on esophageal squamous cell carcinoma-related ring... 
Ring formation | Esophageal cancer-associated lymphatic endothelial cells | VEGF-C | Proliferation | Esophageal squamous carcinoma cells | ring formation | NODE METASTASIS | proliferation | ONCOLOGY | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | LYMPHANGIOGENESIS | EXPRESSION | esophageal squamous carcinoma cells | esophageal cancer-associated lymphatic endothelial cells | Genetic aspects | Research | Vascular endothelial growth factor | Analysis | Esophageal cancer | Endothelium | Care and treatment | Squamous cell carcinoma | Development and progression | Gene expression | Health aspects | Diabetic retinopathy | Lymphatic system | Laboratories | Brain cancer | Metastasis | Kinases | Design | Angiogenesis | Plasmids | Fibroblasts | Diabetes | Prostate | the alive cells quantity in blank control group were the largest | Endostatin group was for the esophageal cancer cells conditional medium of optimum concentration endostatin processed | SG1 plus endostatin group and SG2 plus endostatin group. There was no statistical significance between the comparison of SG1 group and SG2 group | SG1 group | SG1 plus endostatin group and SG2 plus endostatin group (P >0.05). The comparison between rest groups had statistical significance (P | negative plus endostatin group | the three dimensional culture results of negative control group was the highest | of which were the negative plus endostatin group | SG2 group was for the conditional medium of SG2 plasmid transfected esophageal cancer cells | then followed by blank control group | endostatin group | Negative control group was for the conditional medium of empty plasmid transfected esophageal cancer cells | Objective To investigate the effects of VEGF-C inRNA eukaryotic expression vector and expression of endostatin on esophageal squamous carcinoma-related ring formation in vitro and proliferation of lymphatic endothelial cells. Methods KYSE150 cells were subjected to analysis of cell transfection and endostatin operation. The conditional medium was prepared as well. Blank control group was for the conditional medium of esophageal cancer cells without transfection | negative plus endostatin group did not have statistical significance (P>0.05) | SG1 group and negative plus endostatin group | SG1 plus endostatin group and SG2 plus endostatin group. The esophageal cancer related microlymphatic endothelial cells were three-dimensional cultured and was calculated for the various cells formed tube-like structures. CCK-8 assay was employed to detect the cell proliferation condition. Result Generally comparison | SG2 plus endostatin group. The comparsion between SG1 plus endostatin group and SG2 endostatin group did not have statistical significance (P>0.05) | SG2 group and endostation group | SG2 group and negative plus endostatin group | There were also additional three groups | SG2 group | SG1 group was for the conditional medium of SG1 plasmid transfected with esophageal cancer cells | SG1 group and endostation group | the comparison between rest groups had statistical significance (P | then followed by negative control group | the comparsion between every two of SG1 group | SG1 plus endostatin group