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fasp (89) 89
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by Gu, X and Xing, L and Shi, G and Liu, Z and Wang, X and Qu, Z and Wu, X and Dong, Z and Gao, X and Liu, G and Yang, L and Xu, Y
Cell Death and Differentiation, ISSN 1350-9047, 03/2012, Volume 19, Issue 3, pp. 397 - 405
Journal Article
Journal of Proteome Research, ISSN 1535-3893, 01/2019, Volume 18, Issue 1, pp. 217 - 224
Quantification of individual proteins is an essential task in understanding biological processes. For example, determination of concentrations of proteins... 
ADME | total protein approach (TPA) | FASP | MED FASP | stable isotope labeling | quantitative analysis | drug transporter | drug metabolizing enzymes | BIOCHEMICAL RESEARCH METHODS | PROTOCOL | QUANTITATIVE-ANALYSIS | MASS-SPECTROMETRY | TOTAL PROTEIN | PLASMA | ASSAYS | PROTEOMIC ANALYSIS | DEPTH | RULER
Journal Article
Proteomics - Clinical Applications, ISSN 1862-8346, 04/2013, Volume 7, Issue 3-4, pp. 225 - 233
Archival formalin-fixed and paraffin-embedded clinical samples represent a very diverse source of material for proteomic investigation of diseases, often with... 
FASP | Colon cancer | Adenoma | FFPE-tissue | Microdissection | Enzymes | Development and progression | Detergents, Synthetic | Peptides | Formaldehyde
Journal Article
Journal of Proteome Research, ISSN 1535-3893, 08/2018, Volume 17, Issue 8, pp. 2744 - 2754
As a popular sample preparation approach, filter-aided sample preparation (FASP) has been widely used in proteomic analysis. However, several limitations have... 
peptide sequencing | SCAD | LFQ | FASP | Protein extraction | breast cancer | iBAQ | protein identification | enzymatic digestion | urea | PEPTIDE IDENTIFICATION RATES | FASP EFASP | SHOTGUN PROTEOMICS | PROTEIN-ANALYSIS | COVERAGE | QUANTIFICATION | BIOCHEMICAL RESEARCH METHODS | SAMPLE RECOVERY | SPECTROMETRY-BASED PROTEOMICS | MASS-SPECTROMETRY | BREAST-CANCER | peptide sequencing LFQ
Journal Article
Journal of Proteome Research, ISSN 1535-3893, 03/2015, Volume 14, Issue 3, pp. 1637 - 1642
Journal Article
Methods in Enzymology, ISSN 0076-6879, 2017, Volume 585, pp. 15 - 27
Filter-aided sample preparation (FASP) is a versatile and efficient way of processing protein extracts for bottom-up proteomic analysis. The method repurposes... 
Total protein assay | WF-assay | MED-FASP | Protein digestion | Proteomic sample preparation | Filter-aided sample preparation | TOTAL PROTEIN | NUMBER | BIOCHEMISTRY & MOLECULAR BIOLOGY | BIOCHEMICAL RESEARCH METHODS | MULTIENZYME DIGESTION FASP | DEPTH | MASS-SPECTROMETRY | REVEALS | DNA - chemistry | Proteome - analysis | Proteomics - methods | RNA - chemistry
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1841, pp. 3 - 10
Quantitative protein extraction and high-yield generation of peptides from biological samples are the prerequisite for successful bottom-up type proteomic... 
Sample preparation | Multienzyme digestion FASP | Filter-aided sample preparation (FASP) | Protein digestion | Proteomic sample preparation | Bottom-up proteomics | Proteomic reactor
Journal Article
Analytical Chemistry, ISSN 0003-2700, 04/2017, Volume 89, Issue 7, pp. 3840 - 3846
Protein ubiquitination regulates key cellular functions, including protein homeostasis and signal transduction. The digestion of ubiquitinated proteins with... 
DIGESTION | CHEMISTRY, ANALYTICAL | UNIVERSAL | PEPTIDE IDENTIFICATION | PROTEOME | QUANTITATIVE ASSESSMENT | TRYPSIN | FASP | SITES | GLOBAL ANALYSIS | PROTEASE
Journal Article
Methods in Enzymology, ISSN 0076-6879, 2017, Volume 585, pp. 159 - 176
Journal Article
Biological Procedures Online, ISSN 1480-9222, 06/2016, Volume 18, Issue 1, p. 13
Background: Satisfactory sample preparation for mass spectrometry-based analysis is a critical step in the proteomics workflow. The quality and reproducibility... 
IMAC | Phosphoproteomics | Proteomics | FASP | SILAC | Acute myeloid leukemia | Mass spectrometry | CELLS | MASS-SPECTROMETER | PROTEIN-ANALYSIS | QUANTIFICATION | PERFORMANCE | BIOCHEMICAL RESEARCH METHODS | CLASSIFICATION | MULTIENZYME DIGESTION FASP | IDENTIFICATION | WORLD-HEALTH-ORGANIZATION | AML BLASTS | Filtration | Research | Methods
Journal Article
Canadian Journal of Chemical Engineering, ISSN 0008-4034, 2019, Volume 97, Issue 8, pp. 2299 - 2308
Journal Article
Journal of Proteome Research, ISSN 1535-3893, 09/2018, Volume 17, Issue 9, pp. 2917 - 2924
The success of shotgun proteomic analysis depends largely on how samples are prepared. Current approaches (such as those that are gel-, solution-, or filter... 
Sample preparation | Tuberculosis | Proteomics | Sputum | FASP | Suspension trapping | Klebsiella pneumoniae | WORKFLOWS | proteomics | PROTEIN-ANALYSIS | suspension trapping | tuberculosis | BIOCHEMICAL RESEARCH METHODS | sputum | PLATES | sample preparation | BIOMARKERS | DIGESTION | ACTIVE TUBERCULOSIS | DATABASE | URINE | INFECTION
Journal Article
Clinical Proteomics, ISSN 1542-6416, 11/2017, Volume 14, Issue 1, pp. 38 - 8
Background: It is well known that fibrin network binds a large variety of proteins, including inhibitors and activators of fibrinolysis, which may affect clot... 
Fibrin | MED FASP | Clot | Venous thromboembolism | Proteomics | RISK-FACTORS | THROMBOSIS | BIOCHEMICAL RESEARCH METHODS | MULTIENZYME DIGESTION FASP | IDENTIFICATION | PATHOPHYSIOLOGY | TOTAL PROTEIN | CARDIOVASCULAR-DISEASE | CIRCULATING MICROPARTICLES | DEPTH | FIBRINOGEN | Chymotrypsin | Peptides | Analysis | Blood lipids | Thromboembolism | Protein binding
Journal Article
by Kim, H and Dan, K and Shin, H and Lee, J and Wang, JI and Han, D
ANALYTICAL METHODS, ISSN 1759-9660, 09/2019, Volume 11, Issue 36, pp. 4693 - 4698
In-depth proteome profiling still relies widely on extensive off-line peptide fractionation to reduce the complexity of the proteome. However, the... 
CHEMISTRY, ANALYTICAL | SPECTROSCOPY | DRAFT | STRATEGY | FOOD SCIENCE & TECHNOLOGY | ENRICHMENT | FASP | CHROMATOGRAPHY | COMBINATION
Journal Article
Journal Article
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