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Journal Article
Journal of Microbiological Methods, ISSN 0167-7012, 2008, Volume 75, Issue 2, pp. 359 - 361
The Red recombinase system of bacteriophage Lambda has been used to inactivate chromosomal genes in bacteria using PCR products. In this study, we describe the... 
FLP recombinase | Lambda red recombinase | SGI1 | Multidrug-resistant Salmonella
Journal Article
Journal Article
GENETICS, ISSN 0016-6731, 06/2006, Volume 173, Issue 2, pp. 769 - 777
Position effects can complicate transgene analyses. This is especially true when comparing transgenes that have inserted randomly into different genomic... 
REPLACEMENT | CELLS | PHAGE PHI-C31 | P-ELEMENT INSERTIONS | CHROMOSOMAL INTEGRATION | GENETICS & HEREDITY | FLP-RECOMBINASE | TRANSGENE COPLACEMENT | EXPRESSION | LOCUS | EFFICIENT STRATEGY
Journal Article
by Hu, YB and Wang, ZW and Feng, LP and Chen, ZK and Mao, CY and Zhu, Y and Chen, SY
MOLECULAR MICROBIOLOGY, ISSN 0950-382X, 10/2016, Volume 102, Issue 1, pp. 107 - 120
Mycobacterium tuberculosis adopts various strategies to cope with oxidative stress during infection. Transcriptional regulators, including sigma factors, make... 
SURVIVAL | SMEGMATIS | TUBERCULOSIS | BIOCHEMISTRY & MOLECULAR BIOLOGY | IN-VIVO | SIGMA-FACTOR | STREPTOMYCES-COELICOLOR | GENE-EXPRESSION | MICROBIOLOGY | BINDING PROTEIN | FLP RECOMBINASE | RNA-POLYMERASE
Journal Article
Journal Article
PLOS ONE, ISSN 1932-6203, 10/2018, Volume 13, Issue 10, p. e0205538
An engineered phiC31 “Disintegrase” able to make an attP site in Drosophila out of an attR-attL pair is described. This was used to generate attP sites at... 
FLP recombinase | P elements | Drosophila | Insulators | Genomes | Recombinase | Embryos | Enhancers | Position effects | Insects | Plasmids | Genetics | Mutation | Position (location) | Binding sites | Deoxyribonucleic acid--DNA | Deoxyribonucleic acid | DNA
Journal Article
Scientific Reports, ISSN 2045-2322, 12/2017, Volume 7, Issue 1, pp. 17771 - 12
Site-specific recombination systems like those based on the Flp recombinase proved themselves as efficient tools for cell line engineering. The recent... 
CRISPR | FLP recombinase | Recombination | Clonal deletion | Protein folding | Insertion | Recombinase | Nuclease | Gene deletion | Ribonucleic acid--RNA | Index Medicus
Journal Article
Journal Article
Journal Article
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, ISSN 0099-2240, 06/2019, Volume 85, Issue 11, p. 1
The purpose of this study was to create single-copy gene expression systems for use in genomic manipulations of multidrug-resistant (MDR) and extensively... 
BACTERIA | single copy | apramycin | ANTIBIOTIC-RESISTANCE | GENETIC TOOLS | MICROBIOLOGY | ORIGIN | REPLICATION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | zeocin | MINI-TN7 INSERTION | ATTTN7 SITES | EXPRESSION | VECTORS | cloning | gene expression | FLP recombinase | Plasmids | Cloning vectors | Cloning | Apramycin | Markers | Clinical isolates | Fluorescence | Fluorescent indicators | Recombinase | Gene expression | Drug resistance
Journal Article
NATURE COMMUNICATIONS, ISSN 2041-1723, 08/2019, Volume 10, Issue 1, pp. 1 - 15
B cell development is a highly regulated process involving multiple differentiation steps, yet many details regarding this pathway remain unknown. Sequencing... 
YEAST | KEY GENES | DNA | MULTIDISCIPLINARY SCIENCES | TRANSCRIPTION | MECHANISMS | PROLIFERATION | FLP RECOMBINASE | EXPRESSION | LINEAGE | GENOME | DNA biosynthesis | Cell proliferation | Immunization | Animal models | Cell survival | Transcription | Lymphocytes B | Immunodeficiency | Mutation | Saccharomyces cerevisiae | Deoxyribonucleic acid--DNA | Index Medicus
Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 6/2000, Volume 97, Issue 12, pp. 6640 - 6645
We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the... 
Polymerase chain reaction | Biological Sciences | Scars | Plasmids | Genes | Escherichia coli | Antibiotic resistance | Operons | Bacteria | Operator regions | Genetic mutation | Red recombinase | FLP recombinase | FRT Sites | Bacterial genomics | TRANSFORMATION | REPLACEMENT | RECOMBINATION |