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1. Full Text Saturation fluorescence labeling of proteins for proteomic analyses
by Pretzer, Elizabeth and Wiktorowicz, John E
Analytical biochemistry, ISSN 0003-2697, 2008, Volume 374, Issue 2, pp. 250 - 262
Saturation fluorescence labeling | Cys labeling | Two-dimensional electrophoresis | Proteomics | Biochemistry & Molecular Biology | Physical Sciences | Chemistry | Life Sciences & Biomedicine | Biochemical Research Methods | Chemistry, Analytical | Science & Technology | Staining and Labeling - methods | Fluorescent Dyes - chemistry | Humans | Fluorescent Dyes - analysis | Solubility | Electrophoresis, Gel, Two-Dimensional | Linear Models | Proteins - analysis | Thiourea - chemistry | Animals | Phosphines - chemistry | Time Factors | Cattle | Sensitivity and Specificity | Sulfhydryl Compounds - chemistry | Proteins - chemistry | Proteomics - methods | Maleimides - chemistry | Hydrogen-Ion Concentration | Iodoacetamide - chemistry | Proteins | Fluorescence | Index Medicus | saturation fluorescence labeling | two-dimensional electrophoresis | proteomics | cysteine-labeling
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2. Full Text Application of fluorescence difference gel electrophoresis saturation labelling for the analysis of microdissected precursor lesions of pancreatic ductal adenocarcinoma
by Sitek, Barabara and Lüttges, Jutta and Marcus, Katrin and Klöppel, Günter and Schmiegel, Wolff and Meyer, Helmut E and Hahn, Stephan A and Stühler, Kai
Proteomics (Weinheim), ISSN 1615-9853, 07/2005, Volume 5, Issue 10, pp. 2665 - 2679
Fluorescence dye | Pancreatic intra‐epithelial neoplasias | Tissue staining | Microdissection | Pancreatic cancer | Fluorescence difference gel electrophoresis | Differential proteome analysis | Tumour marker | Pancreatic intra-epithelial neoplasias | Fundamental and applied biological sciences. Psychology | Proteins | Gastroenterology. Liver. Pancreas. Abdomen | Miscellaneous | Analytical, structural and metabolic biochemistry | Liver. Biliary tract. Portal circulation. Exocrine pancreas | Biological and medical sciences | Medical sciences | Tumors | Pancreatic Neoplasms - chemistry | Adenocarcinoma - pathology | Carcinoma, Ductal - chemistry | Pancreatic Neoplasms - metabolism | Enzymes - isolation & purification | Humans | Pancreatic Neoplasms - pathology | Electrophoresis, Gel, Two-Dimensional | Neoplasm Proteins - chemistry | Proteome | Adenocarcinoma - metabolism | Neoplasm Proteins - isolation & purification | Sensitivity and Specificity | Cell Line, Tumor | Carcinoma, Ductal - metabolism | Dissection | Adenocarcinoma - chemistry | Index Medicus
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3. Full Text Establishment of “one-piece” large-gel 2-DE for high-resolution analysis of small amounts of sample using difference gel electrophoresis saturation labelling
by Sitek, B and Sipos, B and Pfeiffer, K and Grzendowski, M and Poschmann, G and Hawranke, E and Köper, K and Klöppel, G and Meyer, H E and Stühler, K
Analytical and bioanalytical chemistry, ISSN 1618-2642, 5/2008, Volume 391, Issue 1, pp. 361 - 365
Saturation DIGE | Fluorescence dye | Chemistry | Analytical Chemistry | Biomarker | Food Science | Ecotoxicology | Microdissection | Physical Chemistry | Inorganic Chemistry | Differential proteome analysis | Large two-dimensional electrophoresis (2-DE) | Biochemistry & Molecular Biology | Physical Sciences | Life Sciences & Biomedicine | Biochemical Research Methods | Chemistry, Analytical | Science & Technology | Fluorescent Dyes - chemistry | Proteome - analysis | Proteome - chemistry | Electrophoresis, Gel, Two-Dimensional - methods | Index Medicus
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4. Full Text Novel approaches to analyse glomerular proteins from smallest scale murine and human samples using DIGE saturation labelling
by Sitek, Barbara and Potthoff, Sebastian and Schulenborg, Thomas and Stegbauer, Johannes and Vinke, Tobias and Rump, Lars‐Christian and Meyer, Helmut E and Vonend, Oliver and Stühler, Kai
Proteomics (Weinheim), ISSN 1615-9853, 08/2006, Volume 6, Issue 15, pp. 4337 - 4345
Glomerulus | Laser capture microdissection | DIGE | Differential proteome analysis | Kidney cortex | Biochemistry & Molecular Biology | Life Sciences & Biomedicine | Biochemical Research Methods | Science & Technology | Fundamental and applied biological sciences. Psychology | Proteins | Biological and medical sciences | Miscellaneous | Analytical, structural and metabolic biochemistry | Fluorescent Dyes - chemistry | Kidney Cortex - metabolism | Humans | Proteomics - instrumentation | Fluorescence | Electrophoresis, Gel, Two-Dimensional | Proteins - analysis | Microspheres | Microdissection - methods | Animals | Kidney Glomerulus - metabolism | Mice | Proteins - chemistry | Proteomics - methods | Index Medicus
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5. Full Text Difference gel electrophoresis
by Minden, Jonathan S and Dowd, Susan R and Meyer, Helmut E and Stühler, Kai
Electrophoresis, ISSN 0173-0835, 06/2009, Volume 30, Issue S1, pp. S156 - S161
Biomarker | Fluorescence dye saturation labeling | Microdissection | Differential proteome analysis | 2‐D‐Difference gel electrophoresis | 2-D-Difference gel electrophoresis | Biochemistry & Molecular Biology | Physical Sciences | Chemistry | Life Sciences & Biomedicine | Biochemical Research Methods | Chemistry, Analytical | Science & Technology | Proteins - genetics | Animals | Proteins - metabolism | Humans | Electrophoresis, Gel, Two-Dimensional - methods | Fluorescent Dyes - analysis | Proteins - analysis | Proteomics - methods | Index Medicus
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6. Full Text Two-Dimensional Difference Gel Electrophoresis (2D DIGE)
by Minden, Jonathan S
Methods in cell biology, ISSN 0091-679X, 01/2012, Volume 112, Issue C, pp. 111 - 141
Cy dyes | DIGE | Fluorescence imaging | Two-dimensional electrophoresis | Difference gel electrophoresis | Image analysis | Fluorescent labeling | Minimal labeling | Isoelectric focusing | Saturation labeling | Mass spectrometry | Spot cutting | SDS PAGE
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7. Full Text Application of Fluorescence Dye Saturation Labeling for Differential Proteome Analysis of 1,000 Microdissected Cells from Pancreatic Ductal Adenocarcinoma Precursor Lesions
by Sitek, Barbara and Sipos, Bence and Klöppel, Günter and Schmiegel, Wolff and Hahn, Stephan A and Meyer, Helmut E and Stühler, Kai
2008, Methods in Molecular Biology, ISBN 1603272097, Volume 425
panin, tumor marker | DIGE | fluorescence dye saturation labeling | Difference gel electrophoresis | pancreatic ductal adenocarcinoma | two-dimensional gel electrophoresis | Fluorescent Dyes - chemistry | Proteome | Adenocarcinoma - pathology | Humans | Pancreatic Neoplasms - pathology | Electrophoresis, Gel, Two-Dimensional - methods | Index Medicus
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8. Full Text Identification of crystallin modifications in the human lens cortex and nucleus using laser capture microdissection and CyDye labeling
by Asomugha, C.O and Gupta, R and Srivastava, O.P
Molecular vision, ISSN 1090-0535, 2010, Volume 16, Issue 54-57, pp. 476 - 494
Biochemistry & Molecular Biology | Ophthalmology | Life Sciences & Biomedicine | Science & Technology | Crystallins - metabolism | Crystallins - isolation & purification | Humans | Fluorescence | Lens, Crystalline - metabolism | Electrophoresis, Gel, Two-Dimensional | Microdissection - methods | Cell Nucleus - metabolism | Spectrometry, Mass, Electrospray Ionization | Lasers | Staining and Labeling | Aged | Protein Processing, Post-Translational | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | Lens, Crystalline - cytology | Crystallins - chemistry | Fluorescent Dyes | Index Medicus
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9. Full Text Fluorescent labeling of tetracysteine-tagged proteins in intact cells
by Hoffmann, Carsten and Tsien, Roger Y and Ellisman, Mark H and Gaietta, Guido and Lohse, Martin J and Zürn, Alexander and Adams, Stephen R and Terrillon, Sonia
Nature protocols, ISSN 1754-2189, 09/2010, Volume 5, Issue 10, pp. 1666 - 1677
Biochemistry & Molecular Biology | Life Sciences & Biomedicine | Biochemical Research Methods | Science & Technology | Staining and Labeling - methods | Amino Acid Sequence | Fluoresceins - chemistry | Fluorescent Dyes - chemistry | Humans | Cells, Cultured | Proteins - analysis | Animals | Arsenicals - chemistry | Fluorescence Resonance Energy Transfer | Protein Binding | Organometallic Compounds - chemistry | Proteins - chemistry | Binding Sites | Proteins | Biological research | Fluorescence | Research | Identification and classification | Methods | Biology, Experimental | Index Medicus
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10. Full Text Use of Semiconductor Quantum Dots for Photostable Immunofluorescence Labeling of Cryptosporidium parvum
by Lai Yoke Lee and Say Leong Ong and Jiang Yong Hu and Wun Jern Ng and Yaoyu Feng and Xiaolan Tan and Shih Wei Wong
Applied and Environmental Microbiology, ISSN 0099-2240, 10/2004, Volume 70, Issue 10, pp. 5732 - 5736
Life Sciences & Biomedicine | Microbiology | Biotechnology & Applied Microbiology | Science & Technology | Fundamental and applied biological sciences. Psychology | Biological and medical sciences | Cryptosporidium parvum - pathogenicity | Semiconductors | Water - parasitology | Cryptosporidium parvum - isolation & purification | Fluorescent Antibody Technique - methods | Oocysts - isolation & purification | Antibodies, Protozoan | Cryptosporidium parvum - immunology | Antibodies, Monoclonal | Animals | Fluorescein-5-isothiocyanate | Quantum Dots | Streptavidin | Fluorescent Dyes | Photobleaching | Coccidia | Usage | Research | Quantum theory | Luminescence | Fluorescence | Drinking water | Parasites | Index Medicus | Methods
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11. Full Text Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes
by Bray, Mark-Anthony and Singh, Shantanu and Han, Han and Davis, Chadwick T and Borgeson, Blake and Hartland, Cathy and Kost-Alimova, Maria and Gustafsdottir, Sigrun M and Gibson, Christopher C and Carpenter, Anne E
Nature protocols, ISSN 1754-2189, 09/2016, Volume 11, Issue 9, pp. 1757 - 1774
Biochemistry & Molecular Biology | Life Sciences & Biomedicine | Biochemical Research Methods | Science & Technology | Staining and Labeling - methods | Image Processing, Computer-Assisted | Cell Shape | Humans | Cell Line, Tumor | Fluorescent Dyes - metabolism | Cell Size | Molecular Imaging - methods | Cell culture | Usage | Innovations | Biological assay | Fluorescence microscopy | Methods | Dyes and dyeing | Index Medicus
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12. Full Text A naturally monomeric infrared fluorescent protein for protein labeling in vivo
by Yu, Dan and Baird, Michelle A and Allen, John R and Howe, Elizabeth S and Klassen, Matthew P and Reade, Anna and Makhijani, Kalpana and Song, Yuanquan and Liu, Songmei and Murthy, Zehra and Zhang, Shao-Qing and Weiner, Orion D and Kornberg, Thomas B and Jan, Yuh-Nung and Davidson, Michael W and Shu, Xiaokun
Nature methods, ISSN 1548-7091, 07/2015, Volume 12, Issue 8, pp. 763 - 765
Biochemistry & Molecular Biology | Life Sciences & Biomedicine | Biochemical Research Methods | Science & Technology | Amino Acid Sequence | Fluorescent Dyes - chemistry | Animals, Genetically Modified | Humans | Protein Multimerization | Molecular Sequence Data | Zebrafish | Recombinant Fusion Proteins - chemistry | Plasmids - metabolism | DNA - chemistry | Animals | Transfection | Infrared Rays | Luminescent Proteins - chemistry | Protein Conformation | Mice | Neurons - metabolism | HeLa Cells | Mutation | Developmental Biology | Green Fluorescent Proteins - chemistry | Histidine - chemistry | Drosophila melanogaster | Protein research | Research | Fluorescent proteins | Methods | Fluorescence microscopy | Proteins | Fluorescence | Scientific imaging | Cellular biology | Index Medicus
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