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Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 8/2007, Volume 104, Issue 35, pp. 13936 - 13941
Eukaryotic membrane proteins are often difficult to produce in large quantities, which is a significant obstacle for further structural and biochemical... 
Proteins | Yeasts | Gels | Plasmids | Fluorescence | Cell membranes | Detergents | Solubilization | Membrane proteins | P branes | Structural genomics | GFP-based fusion technology | structural genomics | CRYSTAL-STRUCTURE | MULTIDISCIPLINARY SCIENCES | ESCHERICHIA-COLI | RECEPTOR | IDENTIFICATION | YEAST | COPPER UPTAKE | FUNCTIONAL EXPRESSION | PICHIA-PASTORIS | ENDOPLASMIC-RETICULUM | HETEROLOGOUS PROTEINS | Gene Expression Regulation, Fungal | Recombinant Proteins - metabolism | Membrane Proteins - isolation & purification | Membrane Proteins - genetics | Recombinant Proteins - genetics | Saccharomyces cerevisiae Proteins - genetics | Recombinant Fusion Proteins - metabolism | Recombinant Proteins - isolation & purification | Saccharomyces cerevisiae - metabolism | Microscopy, Confocal | Saccharomyces cerevisiae Proteins - metabolism | Saccharomyces cerevisiae Proteins - isolation & purification | Membrane Proteins - metabolism | Genes, Reporter | Evaluation | Eukaryotes | Genomics | Physiological aspects | Genetic aspects | Research | Chemical properties | Brewer's yeast | Structure | Biochemistry | Organisms | Yeast | Gene expression | Index Medicus | Biological Sciences | Membrane Proteins/genetics/isolation & purification/metabolism | Saccharomyces cerevisiae/metabolism | Microscopy; Confocal | Saccharomyces cerevisiae Proteins/genetics/isolation & purification/metabolism | Recombinant Fusion Proteins/metabolism | Recombinant Proteins/genetics/isolation & purification/metabolism | Gene Expression Regulation; Fungal | Genes; Reporter
Journal Article
Journal of Biological Chemistry, ISSN 0021-9258, 05/2002, Volume 277, Issue 22, pp. 19304 - 19314
Journal Article
Nature, ISSN 0028-0836, 05/2016, Volume 534, Issue 7605, pp. 133 - 137
Ribosome biogenesis is a highly complex process in eukaryotes, involving temporally and spatially regulated ribosomal protein (r-protein) binding and ribosomal... 
60S MATURATION | RNA | BIOGENESIS | PARTICLE | NASCENT RIBOSOMES | MULTIDISCIPLINARY SCIENCES | CRYO-EM | ELECTRON-MICROSCOPY | SACCHAROMYCES-CEREVISIAE | ASSOCIATION | SUBUNIT | RNA, Fungal - ultrastructure | GTP Phosphohydrolases - ultrastructure | Saccharomyces cerevisiae - ultrastructure | DNA, Ribosomal Spacer - metabolism | RNA, Ribosomal - genetics | GTP Phosphohydrolases - chemistry | Saccharomyces cerevisiae - metabolism | Cell Nucleus - metabolism | Ribosome Subunits, Large, Eukaryotic - metabolism | DNA, Ribosomal Spacer - genetics | Base Sequence | DNA, Ribosomal Spacer - ultrastructure | Ribosome Subunits, Large, Eukaryotic - chemistry | Models, Molecular | Ribonucleoproteins - metabolism | Nuclear Proteins - chemistry | Saccharomyces cerevisiae - chemistry | RNA, Ribosomal - ultrastructure | Saccharomyces cerevisiae - cytology | Ribosomal Proteins - isolation & purification | GTP Phosphohydrolases - metabolism | Saccharomyces cerevisiae Proteins - metabolism | GTP-Binding Proteins - metabolism | Saccharomyces cerevisiae Proteins - chemistry | Ribosomal Proteins - chemistry | Ribosome Subunits, Large, Eukaryotic - ultrastructure | Nuclear Proteins - ultrastructure | Molecular Sequence Data | Cytoplasm - metabolism | Ribosomal Proteins - metabolism | Ribosomal Proteins - ultrastructure | Saccharomyces cerevisiae Proteins - isolation & purification | RNA, Fungal - genetics | Active Transport, Cell Nucleus | Ribonucleoproteins - chemistry | Saccharomyces cerevisiae Proteins - ultrastructure | Catalytic Domain | GTP-Binding Proteins - chemistry | RNA, Ribosomal - metabolism | Nuclear Proteins - metabolism | GTP-Binding Proteins - ultrastructure | DNA, Ribosomal Spacer - chemistry | Cell Nucleus - ultrastructure | Rotation | RNA, Fungal - metabolism | Cryoelectron Microscopy | Protein Binding | Cell Nucleus - chemistry | Ribonucleoproteins - ultrastructure | Nucleolus organizer region | Protein research | Research | Ribosomes | Protein binding | Genetic research | Proteins | Polypeptides | Molecular structure | Ribonucleic acid--RNA | Index Medicus
Journal Article
Science, ISSN 0036-8075, 1/2004, Volume 303, Issue 5658, pp. 672 - 676
RNA interference (RNAi) is a widespread silencing mechanism that acts at both the posttranscriptional and transcriptional levels. Here, we describe the... 
Gene silencing | Heterochromatin | Chromatin | RNA | Gels | DNA | Small interfering RNA | Reporter genes | Genetic loci | Reports | Methylation | H3 LYSINE-9 METHYLATION | FISSION YEAST | DOMAIN | EPIGENETIC CONTROL | GENE | HISTONE H3 | MULTIDISCIPLINARY SCIENCES | TRANSCRIPTION | TETRAHYMENA | INTERFERENCE | Endoribonucleases - chemistry | Schizosaccharomyces pombe Proteins - chemistry | Endoribonucleases - genetics | Endoribonucleases - isolation & purification | Molecular Sequence Data | Centromere - metabolism | RNA-Binding Proteins | Cell Cycle Proteins - chemistry | Schizosaccharomyces - genetics | Heterochromatin - metabolism | RNA Interference | Chromosomes, Fungal - metabolism | Mass Spectrometry | Schizosaccharomyces pombe Proteins - metabolism | Cell Cycle Proteins - genetics | Genes, Reporter | Amino Acid Sequence | Cell Cycle Proteins - isolation & purification | Ribonuclease III - metabolism | Schizosaccharomyces pombe Proteins - genetics | Endoribonucleases - metabolism | Cell Cycle Proteins - metabolism | Precipitin Tests | RNA, Fungal - metabolism | Schizosaccharomyces pombe Proteins - isolation & purification | Schizosaccharomyces - metabolism | Argonaute Proteins | Protein Binding | Models, Genetic | Mutation | RNA, Small Interfering - metabolism | Proteins | Yeast | Ribonucleic acid--RNA | Genes | Ago1 protein | RITS complex | Chp1 protein | Dicer protein | Tas3 protein | epigenetics | ribonuclease | Index Medicus | Chromosomes, Fungal | Schizosaccharomyces pombe Proteins | Life Sciences | Centromere | Ribonuclease III | RNA, Fungal | Genetics | RNA, Small Interfering | Cell Cycle Proteins | Schizosaccharomyces
Journal Article
Molecular Cell, ISSN 1097-2765, 02/2013, Volume 49, Issue 4, pp. 583 - 590
Journal Article
Molecular Cell, ISSN 1097-2765, 2002, Volume 10, Issue 3, pp. 457 - 468
The target of rapamycin (TOR) proteins in Saccharomyces cerevisiae, TOR1 and TOR2, redundantly regulate growth in a rapamycin-sensitive manner. TOR2... 
WD-REPEAT PROTEIN | TRANSCRIPTION FACTORS | TARGET | FISSION YEAST | SIGNALING PATHWAYS | BIOCHEMISTRY & MOLECULAR BIOLOGY | CONSERVED SERINE | HEAT REPEATS | ACTIN CYTOSKELETON | TRYPTOPHAN PERMEASE | SACCHAROMYCES-CEREVISIAE | CELL BIOLOGY | Humans | Actins - metabolism | Saccharomyces cerevisiae - drug effects | Tacrolimus Binding Proteins - metabolism | Phosphatidylinositol 3-Kinases | Recombinant Fusion Proteins - metabolism | Tissue Distribution | Glycogen - metabolism | Saccharomyces cerevisiae Proteins - isolation & purification | Fungal Proteins - isolation & purification | Drug Resistance, Fungal | Genes, Reporter | Antifungal Agents - pharmacology | Cell Line | Saccharomyces cerevisiae - physiology | Phosphotransferases (Alcohol Group Acceptor) - isolation & purification | Phosphotransferases (Alcohol Group Acceptor) - genetics | Fungal Proteins - genetics | Saccharomyces cerevisiae Proteins - genetics | Sirolimus - pharmacology | Macromolecular Substances | Phosphotransferases (Alcohol Group Acceptor) - metabolism | Tacrolimus Binding Proteins - genetics | Animals | Saccharomyces cerevisiae Proteins - metabolism | Cytoskeleton - metabolism | Protein Binding | Recombinant Fusion Proteins - genetics | Signal Transduction - physiology | Cell Cycle Proteins | Saccharomyces cerevisiae - growth & development | Fungal Proteins - metabolism | Index Medicus
Journal Article
Biochemistry, ISSN 0006-2960, 05/2017, Volume 56, Issue 19, pp. 2435 - 2445
The formation of the pre-replicative complex (pre-RC) during the G1 phase, which is also called the licensing of DNA replication, is the initial and essential... 
IN-VITRO | ATOMIC-FORCE MICROSCOPY | BUDDING YEAST | CRYOELECTRON MICROSCOPY | BIOCHEMISTRY & MOLECULAR BIOLOGY | REPLICATIVE HELICASE | S-PHASE | SACCHAROMYCES-CEREVISIAE | ORIGIN DNA | AQUEOUS-SOLUTION | PROTEIN COMPLEX | Transcription Factors - chemistry | Minichromosome Maintenance Proteins - metabolism | Protein Multimerization | Origin Recognition Complex - chemistry | DNA, Fungal - chemistry | DNA, Fungal - isolation & purification | Origin Recognition Complex - metabolism | Minichromosome Maintenance Proteins - chemistry | Recombinant Fusion Proteins - metabolism | DNA-Binding Proteins - metabolism | Saccharomyces cerevisiae - metabolism | DNA, Fungal - biosynthesis | Osmolar Concentration | Microscopy, Atomic Force | Minichromosome Maintenance Proteins - genetics | Saccharomyces cerevisiae Proteins - isolation & purification | Nucleic Acid Conformation | Origin Recognition Complex - genetics | Recombinant Proteins - metabolism | Minichromosome Maintenance Proteins - isolation & purification | Models, Molecular | Recombinant Proteins - chemistry | DNA Replication | Recombinant Fusion Proteins - chemistry | Saccharomyces cerevisiae Proteins - genetics | Transcription Factors - genetics | DNA-Binding Proteins - genetics | Origin Recognition Complex - isolation & purification | DNA-Binding Proteins - chemistry | Transcription Factors - metabolism | DNA, Fungal - metabolism | Saccharomyces cerevisiae Proteins - metabolism | Saccharomyces cerevisiae Proteins - chemistry | Atomic force microscopy | Protein biosynthesis | Usage | Research | DNA | Index Medicus
Journal Article
PLoS ONE, ISSN 1932-6203, 09/2017, Volume 12, Issue 9, pp. e0184236 - e0184236
The ATP binding cassette (ABC) transporters Pdr11p and its paralog Aus1p are expressed under anaerobic growth conditions at the plasma membrane of the yeast... 
CELLS | CHOLESTEROL | MULTIDISCIPLINARY SCIENCES | HYDROLYSIS | NUCLEOTIDE-BINDING | ABC TRANSPORTERS | MULTIDRUG-RESISTANCE | PROTEINS | STEROL UPTAKE | P-GLYCOPROTEIN | LIPIDS | Gene Expression | Saccharomyces cerevisiae - genetics | Recombinant Proteins - chemistry | Saccharomyces cerevisiae Proteins - biosynthesis | Cell Membrane - genetics | Recombinant Proteins - genetics | Recombinant Proteins - biosynthesis | Saccharomyces cerevisiae Proteins - genetics | Vanadates - chemistry | Saccharomyces cerevisiae - chemistry | ATP-Binding Cassette Transporters - biosynthesis | ATP-Binding Cassette Transporters - isolation & purification | Cell Membrane - chemistry | Recombinant Proteins - isolation & purification | Saccharomyces cerevisiae - metabolism | ATP-Binding Cassette Transporters - chemistry | Liposomes - chemistry | ATP-Binding Cassette Transporters - genetics | Protein Domains | Saccharomyces cerevisiae Proteins - isolation & purification | Cell Membrane - metabolism | Saccharomyces cerevisiae Proteins - chemistry | Physiological aspects | Genetic aspects | Cell membranes | Research | Carrier proteins | Adenosine triphosphatase | Plasma | Yeast | Laboratories | Protein purification | Fluorescence | Lipids | Biochemistry | Genomes | Orthovanadate | Saccharomyces | pH effects | Proteins | Sterols | Physiology | ABC transporters | Phosphatidylserine | Binding | Growth conditions | Purification | Methionine | Anaerobic conditions | Cholesterol | Environmental science | Mutagenesis | Lysine | Green fluorescent protein | Scientific imaging | Liposomes | ATP | Mass spectrometry | Saccharomyces cerevisiae | Transporter | Index Medicus
Journal Article
Nature Biotechnology, ISSN 1087-0156, 02/2003, Volume 21, Issue 2, pp. 163 - 170
A nonimmune library of 10(9) human antibody scFv fragments has been cloned and expressed on the surface of yeast, and nanomolar-affinity scFvs routinely... 
B-CELLS | FRAGMENTS | PERIPLASMIC EXPRESSION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | HIGH-AFFINITY | PHAGE DISPLAY | GENE REPERTOIRE | T-CELL RECEPTOR | SELECTION | PROTEINS | DIRECTED EVOLUTION | Flow Cytometry - methods | Microchemistry - methods | Saccharomyces cerevisiae - genetics | Humans | Immunoglobulin Variable Region - metabolism | Male | Immunoglobulin Variable Region - immunology | Peptide Library | Feasibility Studies | Polymerase Chain Reaction - methods | Recombinant Proteins - isolation & purification | Saccharomyces cerevisiae - metabolism | Microspheres | Cloning, Molecular | Female | Membrane Proteins - metabolism | Gene Expression Regulation, Fungal | Recombinant Proteins - metabolism | Membrane Proteins - isolation & purification | Saccharomyces cerevisiae - immunology | Membrane Proteins - genetics | Nanotechnology - methods | Cells, Cultured | Membrane Proteins - immunology | Recombinant Proteins - genetics | Immunoglobulin Fragments - immunology | Immunoglobulin Variable Region - isolation & purification | Recombinant Proteins - immunology | Immunoglobulin Fragments - isolation & purification | Immunoglobulin Fragments - genetics | Immunoglobulin Variable Region - genetics | Saccharomyces cerevisiae - isolation & purification | Index Medicus | ANTIBODIES | STABILITY | SACCHAROMYCES CEREVISIAE | b-cells | ANTIGENS | gene repertoire | 60 APPLIED LIFE SCIENCES | T-cell receptor | selection | AFFINITY | proteins | PURIFICATION | YEASTS | SORTING | periplasmic expression | fragments | phage display, high-affinity | directed evolution
Journal Article
Journal of Applied Microbiology, ISSN 1364-5072, 06/2018, Volume 124, Issue 6, pp. 1454 - 1468
Aims: Isolate and characterize a laccase-encoding gene (lac I) of Phlebia brevispora BAFC 633, as well as cloning and expressing cDNA of lac I in Pichia... 
Phlebia brevispora | heterologous expression | gene isolation | characterization | laccase | TRAMETES-VERSICOLOR | SEQUENCES | WHITE-ROT | PLEUROTUS-SAJOR-CAJU | MICROBIOLOGY | COPPER | INDUCTION | RESPONSE ELEMENT | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | MOLECULAR-CLONING | SUPEROXIDE-DISMUTASE | TRANSCRIPTION FACTOR | Fungal Proteins - chemistry | Recombinant Proteins - metabolism | Gene Expression | Polyporales - enzymology | Enzyme Stability | Recombinant Proteins - chemistry | Laccase - chemistry | Recombinant Proteins - genetics | Fungal Proteins - genetics | Polyporales - chemistry | Polyporales - genetics | Protein Sorting Signals | Laccase - genetics | Laccase - metabolism | Laccase - isolation & purification | Pichia - metabolism | Lignin - metabolism | Cloning, Molecular | Pichia - genetics | Polymerase Chain Reaction | Kinetics | Fungal Proteins - isolation & purification | Fungal Proteins - metabolism | Oxidases | Antioxidants | Fungi | Lignin | Peptides | Genes | Genetic research | Genetic transcription | Recombinant proteins | Dyes | Transcription | Gene regulation | Decoloring | Amino acids | Regulatory sequences | Pichia pastoris | Degradation | Proteins | Decolorization | Laccase | Recombinant | Biodegradation | Solvents | Secretion | Cloning | Sodium chloride | Fermentation | Gene expression | Heavy metals | Textile industry wastewaters | Heat shock | Index Medicus
Journal Article