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Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1772, pp. 477 - 490
Transcription activator-like effectors (TALEs) contain programmable DNA-binding domains that can be fused to various effectors to manipulate genetic sequences... 
Plasmid | TAL effector | Gibson assembly | TALEN | Mammalian | Genetic Engineering - methods | Transcription, Genetic - genetics | Gene Library | Transcription Activator-Like Effectors - genetics | DNA-Binding Proteins - genetics | Plasmids - genetics
Journal Article
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1798, pp. 265 - 275
Journal Article
Journal of Biotechnology, ISSN 0168-1656, 01/2020, Volume 307, pp. 193 - 201
Commonly used and well accepted approaches are lacking for site-directed modification of adenoviral vectors. Here, we attempt to introduce an easy-to-implement... 
Gibson assembly | Restriction-ligation cloning | Replication competent | Site-directed mutagenesis | Adenoviral vector | SYSTEM | GENE DELIVERY | PROTECTION | ONRAB(R) | REPLICATION | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | ORAL VACCINATION | PCR
Journal Article
Virus Research, ISSN 0168-1702, 01/2020, Volume 276, p. 197790
(ASGV) belongs to the genus within the family . In this work, we described the construction of full-length infectious cDNA clones of ASGV isolate jilin-shaguo... 
Full-length cDNA clone | Apple stem grooving virus | Gibson cloning | Host range test
Journal Article
ACS Synthetic Biology, ISSN 2161-5063, 05/2018, Volume 7, Issue 5, pp. 1477 - 1480
The ability to rapidly design, build, and test prototypes is of key importance to every engineering discipline. DNA assembly often serves as a rate limiting... 
Gibson assembly | genetic circuits | assembly methods | Golden Gate assembly | DESIGN | BIOCHEMICAL RESEARCH METHODS
Journal Article
Molecular Plant Pathology, ISSN 1464-6722, 10/2019, Volume 20, Issue 10, pp. 1453 - 1462
Summary Transcription Activator‐Like effectors (TALes) represent the largest family of type III effectors among pathogenic bacteria and play a critical role in... 
Gibson assembly | TAL effectors | rice | Xanthomonas | Genetic research | Analysis | Cloning | Genes | Genomics | Methods
Journal Article
Journal of General Virology, ISSN 0022-1317, 11/2018, Volume 99, Issue 11, pp. 1522 - 1527
Beet chlorosis virus (genus Polerovirus, family Luteoviridae), which is persistently transmitted by the aphid Myzus persicae, is part of virus yellows in sugar... 
Gibson assembly | Beet chlorosis virus | Infectious cDNA full-length clone | Polerovirus | SUPPRESSOR | DOMAIN | PROTEIN | APHID TRANSMISSION | MOSAIC-VIRUS | Gibson Assembly | SYSTEMIC INFECTION | VIROLOGY | polerovirus | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | CONSTRUCTION | infectious cDNA full-length clone | WESTERN YELLOWS LUTEOVIRUS | SUGAR-BEET | POTATO-LEAFROLL-VIRUS
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2018, Volume 1671, pp. 203 - 209
Gibson one-step, isothermal assembly method (Gibson assembly) can be used to efficiently assemble large DNA molecules by in vitro recombination involving a... 
Gibson assembly | Streptomyces | High GC content | Antibiotic biosynthetic gene cluster
Journal Article
Molecular & Biochemical Parasitology, ISSN 0166-6851, 11/2016, Volume 210, Issue 1-2, pp. 13 - 21
Journal Article
BMC Biotechnology, ISSN 1472-6750, 06/2018, Volume 18, Issue 1, pp. 37 - 14
Journal Article
Journal of Virological Methods, ISSN 0166-0934, 01/2019, Volume 263, pp. 20 - 23
Viruses belonging to the genus (family ) have circular single-strand DNA genomes encapsidated into quasi-icosahedral particles, and are transmitted by... 
Infectious clones | Seamless cloning | Gibson Assembly | Geminiviridae | Begomovirus | REPLICATION | VIROLOGY | RNA | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | BIOCHEMICAL RESEARCH METHODS | Enzymes | Legumes | Beans | Genomics | Genetic research | Aleyrodidae | Circular DNA | Mimosaceae
Journal Article
Methods in Molecular Biology, ISSN 1064-3745, 2017, Volume 1498, pp. 359 - 366
Site-directed mutagenesis is a commonly used molecular biology technique to manipulate biological sequences, and is especially useful for studying sequence... 
Polymerase chain reaction | Gibson assembly | Escherichia coli | Site-directed mutagenesis | DNA assembly | DNA - genetics | DNA Restriction Enzymes - metabolism | Escherichia coli - genetics | Cloning, Molecular - methods | Mutagenesis - genetics | Mutagenesis, Site-Directed - methods | Polymerase Chain Reaction - methods
Journal Article
ACS SYNTHETIC BIOLOGY, ISSN 2161-5063, 07/2019, Volume 8, Issue 7, pp. 1691 - 1697
Synthetic gene circuits emerge from iterative design-build-test cycles. Most commonly, the time-limiting step is the circuit construction process. Here, we... 
LIBRARIES | synthetic biology | CRISPR | DNA | CONSTRUCTION | BIOCHEMICAL RESEARCH METHODS | Gibson assembly | gene circuit | cloning | TAGS | DNA assembly
Journal Article
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