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Nature Methods, ISSN 1548-7091, 05/2013, Volume 10, Issue 5, pp. 407 - 409
We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma... 
RED | LIFETIMES | CORAL | VARIANTS | BIOCHEMICAL RESEARCH METHODS | ORANGE | Molecular Sequence Data | Green Fluorescent Proteins - metabolism | Chordata - metabolism | Animals | Stochastic Processes | Proteins | Physiological aspects | Fluorescence | Research | Genetics | Cloning | Index Medicus
Journal Article
Nature Structural and Molecular Biology, ISSN 1545-9993, 01/2012, Volume 19, Issue 1, pp. 117 - 122
The use of genetic mutations to study protein functions in vivo is a central paradigm of modern biology. Recent advances in reverse genetics such as RNA... 
TARGET | CELLS | SIGNALING PATHWAYS | BIOCHEMISTRY & MOLECULAR BIOLOGY | TRAP | DROSOPHILA | NONMUSCLE MYOSIN | CELL BIOLOGY | UBIQUITINATION | BIOPHYSICS | GENE | CELLULAR-PROTEINS | EFFICIENT | Drosophila melanogaster - embryology | Cytoskeletal Proteins - genetics | Homeodomain Proteins - metabolism | Humans | Embryo, Nonmammalian - metabolism | Molecular Sequence Data | Embryo, Nonmammalian - embryology | Green Fluorescent Proteins - genetics | Drosophila Proteins - metabolism | Drosophila melanogaster - genetics | Recombinant Fusion Proteins - metabolism | Drosophila melanogaster - metabolism | POU Domain Factors - genetics | Proteolysis | Base Sequence | Antibodies - immunology | Cytoskeletal Proteins - metabolism | POU Domain Factors - metabolism | Wings, Animal - embryology | Amino Acid Sequence | Green Fluorescent Proteins - metabolism | Animals, Genetically Modified | Gene Knockout Techniques | Blotting, Western | Homeodomain Proteins - genetics | Microscopy, Confocal | Wings, Animal - metabolism | Animals | Green Fluorescent Proteins - immunology | Histones - genetics | Models, Biological | Recombinant Fusion Proteins - genetics | Luminescent Proteins - genetics | Drosophila Proteins - genetics | HeLa Cells | Histones - metabolism | Luminescent Proteins - metabolism | Ubiquitin | Physiological aspects | Research | Structure | Green fluorescent protein | Proteins | Genetics | Eukaryotes | Mutation | Insects | Molecular biology | Index Medicus
Journal Article
Journal Article
Nature Communications, ISSN 2041-1723, 03/2016, Volume 7, Issue 1, pp. 11046 - 11046
In addition to the popular method of fluorescent protein fusion, live cell protein imaging has now seen more and more application of epitope tags. The small... 
GFP | SYSTEM | YEAST | LOCALIZATION | MULTIDISCIPLINARY SCIENCES | GLOBAL ANALYSIS | SECRETION | MICROSCOPY | EXPRESSION | LIVING CELLS | Green Fluorescent Proteins - metabolism | Cell Survival | Humans | Protein Multimerization | Staining and Labeling | Fluorescence | Recombinant Fusion Proteins - metabolism | Index Medicus
Journal Article
Nature Cell Biology, ISSN 1465-7392, 07/2015, Volume 17, Issue 7, pp. 893 - 906
LC3-associated phagocytosis (LAP) is a process wherein elements of autophagy conjugate LC3 to phagosomal membranes. We characterize the molecular requirements... 
NONCANONICAL AUTOPHAGY | REGULATE AUTOPHAGY | IMMUNITY | CROHN-DISEASE | INFLAMMATION | MICE | NADPH OXIDASE ACTIVATION | CELLULAR DEFENSE | BINDING | GENOME-WIDE ASSOCIATION | CELL BIOLOGY | Phagosomes - microbiology | Autophagy-Related Proteins | Phosphatidylinositol Phosphates - metabolism | Reactive Oxygen Species - metabolism | Membrane Glycoproteins - metabolism | Microtubule-Associated Proteins - genetics | Microtubule-Associated Proteins - metabolism | Vesicular Transport Proteins - metabolism | NADPH Oxidases - metabolism | Immunoblotting | Male | Green Fluorescent Proteins - genetics | Intracellular Signaling Peptides and Proteins - metabolism | Autophagy | RNA Interference | Tumor Suppressor Proteins - genetics | Aspergillus fumigatus - physiology | NADPH Oxidases - genetics | Female | Intracellular Signaling Peptides and Proteins - genetics | Macrophages - microbiology | Cell Line | Green Fluorescent Proteins - metabolism | Tumor Suppressor Proteins - metabolism | Mice, Inbred C57BL | Phagosomes - metabolism | Vesicular Transport Proteins - genetics | Class III Phosphatidylinositol 3-Kinases - metabolism | Mice, Transgenic | NADPH Oxidase 2 | Membrane Glycoproteins - genetics | Mice, Knockout | Host-Pathogen Interactions | Microscopy, Confocal | Macrophages - metabolism | Animals | Phagocytosis | Cellular proteins | Development and progression | Genetic aspects | Properties | Communicable diseases | Index Medicus
Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 9/2009, Volume 106, Issue 37, pp. 15651 - 15656
Adenosine 5'-triphosphate (ATP) is the major energy currency of cells and is involved in many cellular processes. However, there is no method for real-time... 
Proteins | Pollutant emissions | Mitochondria | Ratios | HeLa cells | Fluorescence | Glycolysis | Dynamic range | Cytoplasm | Oligomycins | Fluorescent indicator | FRET | Live imaging | Oxidative phosphorylation | CANCER-CELLS | F-1-ATPASE BINDS ATP | MITOCHONDRIAL | MULTIDISCIPLINARY SCIENCES | THERMOPHILIC F-1-ATPASE | oxidative phosphorylation | live imaging | MICRODOMAINS | fluorescent indicator | ISOLATED EPSILON-SUBUNIT | CA2 | DYNAMICS | PROTEINS | Humans | Bacterial Proteins - chemistry | Fluorescent Dyes - metabolism | Green Fluorescent Proteins - genetics | Recombinant Fusion Proteins - metabolism | Cell Compartmentation | Adenosine Triphosphate - metabolism | Luminescent Proteins - chemistry | Green Fluorescent Proteins - chemistry | Fluorescent Dyes - chemistry | Green Fluorescent Proteins - metabolism | Bacterial Proton-Translocating ATPases - chemistry | Bacterial Proton-Translocating ATPases - genetics | Bacterial Proton-Translocating ATPases - metabolism | Bacterial Proteins - genetics | Oxidative Phosphorylation | Models, Molecular | Recombinant Fusion Proteins - chemistry | Recombinant Fusion Proteins - genetics | Bacterial Proteins - metabolism | Fluorescence Resonance Energy Transfer - methods | Luminescent Proteins - genetics | HeLa Cells | Microscopy, Fluorescence | Luminescent Proteins - metabolism | Physiological aspects | Research | Adenosine triphosphate | ATP synthases | Glucose | Adenosine triphosphatase | Cells | Nutrition | fluorescence resonance energy transfer | oligomycin A | ATP | Nuclei | Index Medicus | Biological Sciences
Journal Article
Journal Article
Nature Cell Biology, ISSN 1465-7392, 08/2009, Volume 11, Issue 8, pp. 973 - 979
Cellular senescence suppresses cancer by stably arresting the proliferation of damaged cells. Paradoxically, senescent cells also secrete factors that alter... 
TUMOR SUPPRESSION | HUMAN-CELLS | HUMAN FIBROBLASTS | GROWTH-FACTOR | ONCOGENE-INDUCED SENESCENCE | CELLULAR SENESCENCE | CANCER | TUMORIGENESIS | P53 | TELOMERES | CELL BIOLOGY | RNA, Small Interfering - genetics | Humans | Male | Green Fluorescent Proteins - genetics | Tumor Suppressor Protein p53 - genetics | DNA-Binding Proteins - metabolism | Telomerase - genetics | Transfection | Tumor Suppressor Proteins - genetics | Cell Cycle Proteins - genetics | Telomerase - metabolism | Nuclear Proteins - genetics | Protein-Serine-Threonine Kinases - metabolism | Fibroblasts - metabolism | Telomere - genetics | Cell Line | Green Fluorescent Proteins - metabolism | Tumor Suppressor Proteins - metabolism | Enzyme-Linked Immunosorbent Assay | Cell Cycle Proteins - metabolism | Cells, Cultured | Cytokines - secretion | Protein-Serine-Threonine Kinases - genetics | Tumor Suppressor Protein p53 - metabolism | Cellular Senescence - physiology | Cyclin-Dependent Kinase Inhibitor p16 - genetics | Nuclear Proteins - metabolism | Ataxia Telangiectasia Mutated Proteins | DNA-Binding Proteins - genetics | Interleukin-6 - secretion | Blotting, Western | Fibroblasts - radiation effects | Checkpoint Kinase 2 | Cyclin-Dependent Kinase Inhibitor p16 - metabolism | Signal Transduction - physiology | Fibroblasts - cytology | DNA Damage | Microscopy, Fluorescence | Physiological aspects | Cellular signal transduction | Genetic aspects | Research | Cytokines | DNA damage | Index Medicus
Journal Article