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1. Full Text A phenotypic comparison of osteoblast cell lines versus human primary osteoblasts for biomaterials testing
by Czekanska, E. M and Stoddart, M. J and Ralphs, J. R and Richards, R. G and Hayes, J. S
Journal of Biomedical Materials Research Part A, ISSN 1549-3296, 08/2014, Volume 102, Issue 8, pp. 2636 - 2643
Immortalized cell lines are used more frequently in basic and applied biology research than primary bone‐derived cells because of their ease of access and... 
differentiation | osteoblast | cell line | primary cell | In vitro cell model | MOUSE CALVARIA | MATERIALS SCIENCE, BIOMATERIALS | ENGINEERING, BIOMEDICAL | REPRESENTATIVE MODELS | MC3T3-E1 CELLS | PROLIFERATION | CULTURE | MG-63 OSTEOSARCOMA CELLS | OSTEOGENESIS IN-VITRO | MINERALIZATION | BONE | Cell Line | Cell Proliferation | Calcification, Physiologic - drug effects | Osteoblasts - enzymology | Cell Count | Humans | Osteoblasts - drug effects | Alkaline Phosphatase - metabolism | Biocompatible Materials - pharmacology | Gene Expression Regulation - drug effects | Materials Testing - methods | Phenotype | Animals | Models, Biological | Mice | Osteoblasts - cytology | Comparative analysis | Biological products | Human | Surgical implants | Biotechnology | Biomedical materials | Analogies | Biocompatibility | In vitro testing | Magnesium | Biomaterials
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2. Full Text Viscoelastic properties of human mesenchymally-derived stem cells and primary osteoblasts, chondrocytes, and adipocytes
by Darling, Eric M and Topel, Matthew and Zauscher, Stefan and Vail, Thomas P and Guilak, Farshid
Journal of Biomechanics, ISSN 0021-9290, 2007, Volume 41, Issue 2, pp. 454 - 464
Abstract The mechanical properties of single cells play important roles in regulating cell-matrix interactions, potentially influencing the process of... 
Physical Medicine and Rehabilitation | Atomic force microscopy | Adipocyte | Chondrocyte | ADAS cell | Osteoblast | Mechanotransduction | MSC | Cell mechanics | MATRIX | MECHANOBIOLOGY | ENGINEERING, BIOMEDICAL | cell mechanics | mechanotransduction | osteoblast | atomic force microscopy | MECHANICAL-PROPERTIES | CULTURE | DEFORMATION | CARTILAGE | chondrocyte | ATOMIC-FORCE MICROSCOPY | BIOPHYSICS | adipocyte | ARTICULAR CHONDROCYTES | DIFFERENTIATION | Viscosity | Humans | Middle Aged | Osteoblasts - physiology | Cells, Cultured | Elasticity | Male | Adipocytes - physiology | Compressive Strength | Chondrocytes - physiology | Adult | Female | Aged | Mesenchymal Stromal Cells - physiology | Biomechanics | Signal transduction | Genotype & phenotype | Stem cells | Bone marrow | Biomarkers | Mechanical properties | Gene therapy | Joint replacement surgery | Methods
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3. Full Text Nodule formation and mineralisation of human primary osteoblasts cultured on a porous bioactive glass scaffold
by Gough, Julie Elizabeth and Jones, Julian R and Hench, Larry L
Biomaterials, ISSN 0142-9612, 2004, Volume 25, Issue 11, pp. 2039 - 2046
The aim of this study was to analyse human osteoblast responses to a porous bioactive glass scaffold. It was hypothesised that osteoblasts would attach,... 
Osteoblast | Bioactive glass | Ion release | Mineralisation | Apoptosis | VITRO | MATERIALS SCIENCE, BIOMATERIALS | ENGINEERING, BIOMEDICAL | apoptosis | PROLIFERATION | osteoblast | bioactive glass | DISSOLUTION | BONE-FORMATION | mineralisation | CALCIUM | INTRACELLULAR ACIDIFICATION | DIFFERENTIATION | ion release | IN-VIVO EVALUATION | IONIC PRODUCTS | Tissue Engineering - methods | Biocompatible Materials - chemistry | Humans | Osteoblasts - physiology | Cells, Cultured | Calcification, Physiologic - physiology | Materials Testing | Glass - chemistry | Cell Culture Techniques - methods | Cell Division - physiology | Cell Adhesion - physiology | Surface Properties | Apoptosis - physiology | Osteoblasts - cytology | Porosity
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4. Full Text BiodentineTM is cytocompatible with human primary osteoblasts
by Scelza, Miriam Zaccaro and Nascimento, Joyce Costa and Silva, Licinio Esmeraldo da and Gameiro, Vinícius Shott and DE Deus, Gustavo and Alves, Gutemberg
Brazilian oral research, ISSN 1806-8324, 09/2017, Volume 31, p. e81
Calcium silicate-based materials have been widely studied due to their resemblance to, and similar applicability of, mineral trioxide aggregate (MTA). Among... 
Cell Survival - drug effects | Reproducibility of Results | Cell Count | Humans | Osteoblasts - drug effects | Cells, Cultured | Materials Testing | Biocompatible Materials - pharmacology | Oxides - pharmacology | Time Factors | Statistics, Nonparametric | Aluminum Compounds - pharmacology | Cell Proliferation - drug effects | Pulp Capping and Pulpectomy Agents - pharmacology | Drug Combinations | Calcium Compounds - pharmacology | Silicates - pharmacology | Osteoblasts | Dentin, In Vitro Techniques
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5. Full Text Biocompatibility of polypyrrole with human primary osteoblasts and the effect of dopants
by Fahlgren, A and Bratengeier, C and Gelmi, A and Semeins, C.M and Klein-Nulend, J and de Jager, E.W.H and Bakker, A.D and Medicinska fakulteten and Institutionen för fysik, kemi och biologi and Linköpings universitet and Institutionen för klinisk och experimentell medicin and Tekniska fakulteten and Biosensorer och bioelektronik and Avdelningen för cellbiologi
PLoS ONE, ISSN 1932-6203, 07/2015, Volume 10, Issue 7, p. e0134023
Polypyrrole (PPy) is a conducting polymer that enables controlled drug release upon electrical stimulation. We characterized the biocompatibility of PPy with... 
ELECTROCHEMISTRY | CHONDROITIN SULFATE | NANOPARTICLES | INHIBITION | OSTEOCALCIN | MULTIDISCIPLINARY SCIENCES | SURFACE | TOPOGRAPHY | DIFFERENTIATION | EXPRESSION | CONDUCTING POLYMERS | Pyrroles - pharmacology | Wettability | Cell Proliferation | Humans | Osteoblasts - drug effects | Surface Properties | Cells, Cultured | Polymers - pharmacology | Biocompatible Materials | Osteoblasts - cytology | Cell Adhesion | Muscle proteins | Polymers | Gene expression | Sulfates | Health aspects | Alkaline phosphatase | Surgical implants | Drug delivery systems | Transplants & implants | Cytology | Biology | Joint surgery | Osteoblasts | Cell morphology | Electrodes | Dental restorative materials | Biomedical materials | Bone growth | Filaments | Actin | Topography | Biocompatibility | Polypyrroles | Controlled release | Gold | Osteocalcin | Toluene | Polymerization | Osteopontin | Contact angle | Dental prosthetics | Electrical stimuli | Conducting polymers | Antibiotics | Cell number | Vinculin | Stem cells | Dopants | Chondroitin sulfate | Sulfate | Dental implants | Biosensors | Osteogenesis | Cell and Molecular Biology | Basic Medicine | Medical and Health Sciences | Medicin och hälsovetenskap | Cell- och molekylärbiologi | Medicinska och farmaceutiska grundvetenskaper
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6. Full Text The use of nanoscale topography to modulate the dynamics of adhesion formation in primary osteoblasts and ERK/MAPK signalling in STRO-1+ enriched skeletal stem cells
by Biggs, Manus J.P and Richards, R. Geoff and Gadegaard, Nikolaj and Wilkinson, Chris D.W and Oreffo, Richard O.C and Dalby, Matthew J
Biomaterials, ISSN 0142-9612, 2009, Volume 30, Issue 28, pp. 5094 - 5103
Abstract The physiochemical characteristics of a material with in vivo applications are critical for the clinical success of the implant and regulate both... 
Advanced Basic Science | Dentistry | Nanotopography | Focal adhesions | ERK/MAPK | Osteoblasts | Mesenchymal stem cells | MATERIALS SCIENCE, BIOMATERIALS | ACTIVATED PROTEIN-KINASE | FLUID-FLOW | FIBROBLAST | ENGINEERING, BIOMEDICAL | OSTEOGENIC DIFFERENTIATION | TRANSDUCTION PATHWAYS | MECHANICAL STRAIN | BONE-FORMATION | GROWTH | GENE-EXPRESSION | EXTRACELLULAR-MATRIX | Mesenchymal Stromal Cells - cytology | Nanotechnology - methods | Humans | Cells, Cultured | Mesenchymal Stromal Cells - metabolism | Nanostructures - chemistry | Osteoblasts - cytology | Osteoblasts - metabolism | Cell Adhesion | Microtechnology | MAP Kinase Signaling System | Biological products | Actin | Stem cells | Muscle proteins | Nanotechnology | Topographical drawing | Integrins
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7. Full Text MET overexpression turns human primary osteoblasts into osteosarcomas
by Patanè, Salvatore and Avnet, Sofia and Coltella, Nadia and Costa, Barbara and Sponza, Simone and Olivero, Martina and Vigna, Elisa and Naldini, Luigi and Baldini, Nicola and Ferracini, Riccardo and Corso, Simona and Giordano, Silvia and Comoglio, Paolo M and Di Renzo, Maria Flavia
Cancer Research, ISSN 0008-5472, 05/2006, Volume 66, Issue 9, pp. 4750 - 4757
The MET oncogene was causally involved in the pathogenesis of a rare tumor, i.e., the papillary renal cell carcinoma, in which activating mutations, either... 
LYMPH-NODE METASTASES | INVASIVE GROWTH | ONCOLOGY | PAPILLARY RENAL-CARCINOMAS | HEPATOCYTE GROWTH-FACTOR | MET/HGF RECEPTOR | MUSCULOSKELETAL TUMORS | FACTOR RECEPTOR GENE | SOMATIC MUTATIONS | HGF RECEPTOR | KINASE DOMAIN | Humans | Proto-Oncogene Proteins - biosynthesis | Bone Neoplasms - pathology | Bone Neoplasms - metabolism | Receptors, Growth Factor - genetics | Cell Transformation, Neoplastic - genetics | Female | Bone Neoplasms - genetics | Oncogenes | Osteosarcoma - metabolism | Gene Expression | Osteoblasts - physiology | Proto-Oncogene Proteins c-met | Proto-Oncogene Proteins - genetics | Mice, SCID | Cell Transformation, Neoplastic - metabolism | Osteoblasts - pathology | Animals | Receptors, Growth Factor - biosynthesis | Cell Line, Tumor | Mice | Osteosarcoma - genetics | Cell Transformation, Neoplastic - pathology | Osteoblasts - metabolism | Osteosarcoma - pathology
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8. Full Text Strontium ranelate treatment of human primary osteoblasts promotes an osteocyte-like phenotype while eliciting an osteoprotegerin response
by Atkins, G J and Atkins, G J and Welldon, K J and Welldon, K J and Halbout, P and Halbout, P and Findlay, D M and Findlay, D M
Osteoporosis International, ISSN 0937-941X, 4/2009, Volume 20, Issue 4, pp. 653 - 664
The effect of strontium ranelate (SR) on human osteoblast differentiation was tested. SR induced osteoblastic proliferation, in vitro mineralization, and... 
Medicine & Public Health | Gynecology | Orthopedics | Rheumatology | OPG | Osteoblast | Strontium ranelate | DMP-1 | Sclerostin | Osteocyte | Endocrinology | VERTEBRAL FRACTURE | RESORPTION IN-VITRO | DENTIN MATRIX PROTEIN-1 | MESSENGER-RNA | BONE-FORMATION | OSTEOCLAST FORMATION | ENDOCRINOLOGY & METABOLISM | MECHANICAL STIMULATION | RECEPTOR ACTIVATOR | MARROW STROMAL CELLS | KAPPA-B LIGAND | Calcification, Physiologic - genetics | Osteocytes - drug effects | Calcification, Physiologic - drug effects | Osteocytes - metabolism | Apoptosis - drug effects | Humans | Osteoblasts - drug effects | Osteoprotegerin - biosynthesis | Cells, Cultured | Thiophenes - pharmacology | Bone Density Conservation Agents - pharmacology | Dose-Response Relationship, Drug | Gene Expression Regulation - drug effects | Phenotype | Cell Differentiation - drug effects | Female | Cell Proliferation - drug effects | Osteoblasts - cytology | Osteoblasts - metabolism | Organometallic Compounds - pharmacology | Osteoporosis | Care and treatment | Medical examination | Physiological aspects | Postmenopausal women | Genetic aspects | Research | Osteoblasts | Diseases | Drug therapy | Womens health | Menopause | DMP
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9. Full Text CXCL8 and CCL20 enhance osteoclastogenesis via modulation of cytokine production by human primary osteoblasts
by Pathak, J.L and Bakker, A.D and Verschueren, P and Lems, W.F and Luyten, F.P and Klein-Nulend, J and Bravenboer, N
PLoS ONE, ISSN 1932-6203, 2015, Volume 10, Issue 6, p. e0131041
Generalized osteoporosis is common in patients with inflammatory diseases, possibly because of circulating inflammatory factors that affect osteoblast and... 
GENERALIZED BONE LOSS | CELLS | IN-VITRO | CROHNS-DISEASE | MECHANICAL-STRESS | MULTIDISCIPLINARY SCIENCES | NITRIC-OXIDE | NECROSIS-FACTOR-ALPHA | RHEUMATOID-ARTHRITIS PATIENTS | EXPRESSION | INTERLEUKIN-8 IL-8 | Interleukin-6 - antagonists & inhibitors | Extracellular Matrix Proteins - biosynthesis | Humans | Middle Aged | Culture Media, Conditioned - pharmacology | Interleukin-8 - physiology | Male | Osteoclasts - cytology | Arthritis, Rheumatoid - complications | Osteoporosis - etiology | Bone Remodeling - drug effects | Chemokine CCL20 - pharmacology | Chemokine CCL20 - physiology | Receptors, CCR6 - metabolism | Female | Interleukin-8 - pharmacology | Osteoporosis - physiopathology | Arthritis, Rheumatoid - physiopathology | Bone Resorption - physiopathology | Cytokines - metabolism | Interleukin-6 - genetics | Extracellular Matrix Proteins - genetics | Osteoblasts - drug effects | Cells, Cultured | Cell Division - drug effects | Gene Expression Regulation - drug effects | Tumor Necrosis Factor-alpha - pharmacology | Cell Differentiation - drug effects | Receptors, Interleukin-8A - metabolism | Interleukin-6 - biosynthesis | Aged | Osteoblasts - metabolism | Proteins | Rheumatoid factor | Osteoporosis | Cytokines | Gene expression | Analysis | Osteoprogenitor cells | Disease | Pathogenesis | Biology | Arthritis | Cell interactions | Osteoblasts | Nuclei | Inflammatory diseases | Interleukin 6 | Protein folding | Rodents | CCL20 protein | Tumor necrosis factor-TNF | Biocompatibility | Conditioning | Cloning | Inflammation | Bone turnover | Dentistry | Metabolism | Serum levels | Osteoclastogenesis | Osteoblastogenesis | Engineering research | Rheumatoid arthritis | Ligands | Osteoclasts | Bone | Chemokines
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10. Full Text Pellet culture model for human primary osteoblasts
by Jähn, K and Richards, R.G and Archer, C.W and Stoddart, M.J
European Cells and Materials, ISSN 1473-2262, 2010, Volume 20, pp. 149 - 161
In vitro monolayer culture of human primary osteoblasts (hOBs) often shows unsatisfactory results for extracellular matrix deposition, maturation and... 
Osteoblast | 3D | Differentiation | Pellet culture | Osteocyte | OSTEOCYTES | 3-DIMENSIONAL CULTURES | MATERIALS SCIENCE, BIOMATERIALS | differentiation | BIOCHEMISTRY & MOLECULAR BIOLOGY | HUMAN-BONE | PROLIFERATION | osteocyte | CELL-LINES | pellet culture | IN-VITRO | OSTEOCALCIN | EXTRACELLULAR-MATRIX | EXPRESSION | Humans | Middle Aged | Cells, Cultured | Extracellular Matrix - metabolism | Male | RNA, Messenger - metabolism | Cell Culture Techniques - methods | Phenotype | Cell Division | Female | Cell Differentiation | Osteoblasts - cytology | Osteoblasts - metabolism
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11. Full Text Type V OI Primary Osteoblasts Display Increased Mineralization Despite Decreased COL1A1 Expression
by Reich, Adi and Bae, Alison S and Barnes, Aileen M and Cabral, Wayne A and Hinek, Aleksander and Stimec, Jennifer and Hill, Suvimol C and Chitayat, David and Marini, Joan C
The Journal of Clinical Endocrinology & Metabolism, ISSN 0021-972X, 02/2015, Volume 100, Issue 2, pp. E325 - E332
Context: Patients with type V osteogenesis imperfecta (OI) are heterozygous for a dominant IFITM5 c.-14C>T mutation, which adds five residues to the N terminus... 
IMPERFECTA TYPE-V | IFITM5 MUTATION | 5'-UTR | DEPOSITION | SERPINF1 | ENDOCRINOLOGY & METABOLISM | PHENOTYPIC VARIABILITY | OSTEOGENESIS-IMPERFECTA | I COLLAGEN | HYPERPLASTIC CALLUS | DEFICIENCY | Calcinosis - genetics | Collagen Type I - metabolism | Osteogenesis Imperfecta - metabolism | Membrane Proteins - genetics | Humans | Middle Aged | Child, Preschool | Osteogenesis Imperfecta - genetics | Male | Young Adult | Osteoblasts - pathology | Collagen Type I - genetics | Adult | Female | Osteogenesis Imperfecta - pathology | Aged | Membrane Proteins - metabolism | Mutation | Calcinosis - metabolism | Calcinosis - pathology | Osteoblasts - metabolism | Advances in Genetics | JCEM Online
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