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Angewandte Chemie, ISSN 0044-8249, 07/2013, Volume 125, Issue 29, pp. 7743 - 7746
Bloß nicht loslassen: Die Co3+‐vermittelte Wechselwirkung zwischen Nitrilotriessigsäure und His6‐Tag ist so stabil und inert gegen Ligandensubstitution, dass... 
Reaktionskinetik | Nitrilotriessigsäure | His‐Tag | Cobalt | Proteinmodifikationen
Journal Article
by Xie, H and Yang, C and Chen, LE
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, ISSN 1000-3282, 10/2009, Volume 36, Issue 10, pp. 1364 - 1369
Using gene fusion technology, polypeptide fusion tags can be engineered into target proteins at the genetic level. The resultant recombinant proteins may... 
fusion proteins | ONE-STEP PURIFICATION | MECHANISM | BIOCHEMISTRY & MOLECULAR BIOLOGY | ESCHERICHIA-COLI | AFFINITY TAGS | intein | ENTEROKINASE CLEAVAGE | HIS-TAGS | DEHYDROGENASE | polypeptide fusion tags | protease | tag removal | BIOPHYSICS | RECOMBINANT PROTEINS | MINI-INTEIN | EXPRESSION
Journal Article
分析化学, ISSN 0253-3820, 2009, Volume 37, Issue A02, pp. 13 - 13
His-Tag标签蛋白是目前最常见的一种融合蛋白表达方式,其纯化主要采用金属螯合亲和色谱法(MCAC)。方法具有配基简单、分离条件温和、通用性强等优点。但也有操作时间较长,富集效率低等不足。本文采用水相合成法制备了Fe3O4/Au核/壳型纳米材料, 
Fe3O4 | 简便 | 融合蛋白表达 | 纳米材料 | 分离条件 | 氮川三乙酸 | His-Tag | 组氨酸
Journal Article
by Zhu, ZY and Bo, T and Zhang, WH and Zhang, HJ
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, ISSN 1000-3282, 07/2004, Volume 31, Issue 7, pp. 655 - 658
His-tagged Chinese hamster dihydrofolate reductase ( DHFR) expression in pET vector system has been reported very low. A newly modified pET protein expression... 
pET-DB vector | PROTEIN | BIOPHYSICS | BIOCHEMISTRY & MOLECULAR BIOLOGY | His-tag | protein expression | dihydrofolate reductase
Journal Article
D-型胺基酸氧化酶(D-amino acid oxidase;EC 1.4.3.3;DAO)為一種以FAD為輔基之氧化還原酵素,能專一性催化D-型胺基酸進行氧化脫氨作用,產生相對之α-keto... 
D-型胺基酸氧化酶 | DAO | His-tag
Dissertation
本研究中以E. coli BL21(DE3) 作為表達重組蛋白his-tag... 
卵白素 | his-tag streptavidin | purification | his-tag鏈黴卵白素 | 純化 | Avidin | 最適條件 | optimal conditions
Dissertation
福井大学医学部研究雑誌, ISSN 1348-8562, 12/2004, Volume 5, pp. 1 - 5
By TSKgel BioAssist Co^<2+>-Chelate Chromatography using a linear gradient of imidazole, His-tag-NusG protein was purified as a monomer, dimer, trimer and... 
Journal Article
鯉魚(Cyprinus carpio)是日常生活中常見可以生活在渾濁水池的魚 ,為了瞭解其視覺生理,將已被篩選到的鯉魚視紫質 (rhodopsin) cDNA 以PCR 轉錄區接入大腸桿菌表現載體,構築成pET32-Rho。這個重組視紫 質的胺端與thioredoxin 接成融合蛋白,銜接處並有6個組胺酸(... 
rhodopsin | his-tag | 視紫質 | 鯉魚 | baculovirus | 桿狀病毒 | 大腸桿菌 | common carp | 組胺酸標識 | E. coli
Dissertation
本論文於酸性條件下,將三區塊共聚高分子界面活性劑P123當作模板,利用氫鍵的作用力與帶正電荷的矽源四氧乙基矽酯(tetraethyl... 
組胺酸標籤 | 中孔洞分子篩 | protein purification | mesoporous seieve | SBA-15 | 蛋白質純化 | 6×-His tag
Dissertation
本論文之研究重點在於探討親和標籤對重組DCase ( N-carbamoyl-D-amino acid amidohydrolase ) 酵素之生產表現及活性之影響。在此研究中,以基因工程技術,將先前本實驗室所建構的DCase2重組酵素C端之6 ×His tag金屬親和標籤移除;或將6 ×His... 
金屬親和標籤 | 6 ×His tag | N-carbamoyl-D-amino acid amidohydrolase
Dissertation
日本イオン交換学会誌, ISSN 0915-860X, 2008, Volume 19, Issue 2, pp. 101 - 106
An immobilized metal affinity porous sheet was prepared by radiation-induced graft polymerization and subsequent chemical modifications. A 2-mm thick porous... 
ニッケルイオン | タンパク質精製 | 多孔性シート | 固定化金属アフィニティ | His-tag融合タンパク質
Journal Article
粒細胞增生因子(G-CSF)是血球形成過程當中不可或缺的細胞激素,它可以刺激嗜中性白血球(neutrophil)的增生及分化且具有高度的專一性。基因重組的粒細胞增生因子在今日已廣泛的使用在治療各種原因所引發的嗜中性白血球缺乏症以及需要接受骨髓移植的病人身上,是目前市面上最成功的基因重組蛋白質藥物之一。... 
粒細胞增生因子 | pET表現系統 | His-tag | 大腸桿菌 | pET expression system | enterokinase | T7 promoter | T7 啟動子 | 腸激酶 | G-CSF | E. coli
Dissertation
日本蠶絲學雜誌, ISSN 0037-2455, 04/2005, Volume 74, Issue 1, pp. 15 - 20
Journal Article
日本蚕糸学雑誌, ISSN 0037-2455, 2005, Volume 74, Issue 1, pp. 15 - 20
Silkworm acidic ribosomal proteins P0, P1 and P2 (P proteins) were fused with His-tag at the C-terminal end. Each protein was expressed in BmN4 cells... 
ribosomal P proteins | BmN 4 cells | transfection | His-tag
Journal Article
SPR是一新型光學感測器,近年來被廣泛應用於生物科技來研究生物分子間的相互作用。本研究利用德州儀器(TI)出品價格較便宜的SPR探討各類生物分子間的作用情形,由於TI之SPR感測金面上僅有一金膜,因此在固定化探針前必須將感測面加以修飾,增加表面上之官能基以利於探針之固定化,本論文首先研究在硫醇修飾層及在Con... 
thrombin | self assembled monolayer(SAM) | Con A | 抗凝血蛋白酵素 | 光學感測器 | Anti-his tag antibody | 核酸配體 | 半刀豆球蛋白 | SPR | GM-CSF | aptamer | 抗組胺酸標籤抗體 | 自我聚集單層 | dextran | 葡聚糖
Dissertation
Journal Article
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