Glycoconjugate Journal, ISSN 0282-0080, 10/2017, Volume 34, Issue 5, pp. 643 - 649
There is a need for degradative enzymes in the study of glycosaminoglycans. Many of these enzymes are currently available either in their natural or...
Life Sciences | Biochemistry, general | Keratan sulfate, glycosaminoglycan | Pathology | Protein engineering | Hydrolase | Keratanase II | Mass spectrometry | Keratan sulfate | SULFATE | BIOCHEMISTRY & MOLECULAR BIOLOGY | PURIFICATION | glycosaminoglycan | BINDING | Bacterial Proteins - chemistry | Isoenzymes - chemistry | Structure-Activity Relationship | Acetylglucosaminidase - genetics | Keratan Sulfate - chemistry | Acetylglucosaminidase - metabolism | Isoenzymes - metabolism | Cloning, Molecular | Escherichia coli - metabolism | Protein Engineering | Recombinant Proteins - metabolism | Amino Acid Sequence | Gene Expression | Isoenzymes - genetics | Bacterial Proteins - genetics | Enzyme Stability | Keratan Sulfate - metabolism | Recombinant Proteins - chemistry | Bacillus - genetics | Recombinant Proteins - genetics | Plasmids - metabolism | Hydrolysis | Bacillus - enzymology | Escherichia coli - genetics | Acetylglucosaminidase - chemistry | Plasmids - chemistry | Bacterial Proteins - metabolism | Kinetics | Glycosaminoglycans | Sulfates | Escherichia coli | Resveratrol | Biomedical engineering | Biodegradation | Enzymes | N-Acetylglucosaminidase | E coli | Bacteria | Shelf life | Sulfate | Thermal stability | Mutants | Recombinant
Life Sciences | Biochemistry, general | Keratan sulfate, glycosaminoglycan | Pathology | Protein engineering | Hydrolase | Keratanase II | Mass spectrometry | Keratan sulfate | SULFATE | BIOCHEMISTRY & MOLECULAR BIOLOGY | PURIFICATION | glycosaminoglycan | BINDING | Bacterial Proteins - chemistry | Isoenzymes - chemistry | Structure-Activity Relationship | Acetylglucosaminidase - genetics | Keratan Sulfate - chemistry | Acetylglucosaminidase - metabolism | Isoenzymes - metabolism | Cloning, Molecular | Escherichia coli - metabolism | Protein Engineering | Recombinant Proteins - metabolism | Amino Acid Sequence | Gene Expression | Isoenzymes - genetics | Bacterial Proteins - genetics | Enzyme Stability | Keratan Sulfate - metabolism | Recombinant Proteins - chemistry | Bacillus - genetics | Recombinant Proteins - genetics | Plasmids - metabolism | Hydrolysis | Bacillus - enzymology | Escherichia coli - genetics | Acetylglucosaminidase - chemistry | Plasmids - chemistry | Bacterial Proteins - metabolism | Kinetics | Glycosaminoglycans | Sulfates | Escherichia coli | Resveratrol | Biomedical engineering | Biodegradation | Enzymes | N-Acetylglucosaminidase | E coli | Bacteria | Shelf life | Sulfate | Thermal stability | Mutants | Recombinant
Journal Article
Glycoconjugate Journal, ISSN 0282-0080, 12/2017, Volume 34, Issue 6, pp. 789 - 795
Recently, we established a mouse monoclonal antibody specific to hiPS/ hES cells, R-10G, which recognizes a type of keratan sulfate. Keratan sulfates (KS)...
Life Sciences | Biochemistry, general | Pathology | Induced pluripotent stem (iPS) cells | R-10G | Keratan sulfates | Keratanase II | TRA-1-60 | N -acetyllactosamine tetrasaccharides | N-acetyllactosamine tetrasaccharides | SERIES | BIOCHEMISTRY & MOLECULAR BIOLOGY | SULFATE PROTEOGLYCAN | ANTIGENS | DIGESTION | 3 MONOCLONAL-ANTIBODIES | HUMAN TERATOCARCINOMA CELLS | EMBRYONAL CARCINOMA-CELLS | Glycosaminoglycans | Polyethylene glycol | Monoclonal antibodies | Cell differentiation | Sulfates | Polyols | Cells | Antigenic determinants | Binding | Polyethylene | Keratan sulfate | N-acetyllactosamine | Oligosaccharides | Avidin | Substrates | Glycan | Sulfation | Substrate specificity | Rodents | Sulfate | Chemical synthesis | Enzyme-linked immunosorbent assay | Binding sites
Life Sciences | Biochemistry, general | Pathology | Induced pluripotent stem (iPS) cells | R-10G | Keratan sulfates | Keratanase II | TRA-1-60 | N -acetyllactosamine tetrasaccharides | N-acetyllactosamine tetrasaccharides | SERIES | BIOCHEMISTRY & MOLECULAR BIOLOGY | SULFATE PROTEOGLYCAN | ANTIGENS | DIGESTION | 3 MONOCLONAL-ANTIBODIES | HUMAN TERATOCARCINOMA CELLS | EMBRYONAL CARCINOMA-CELLS | Glycosaminoglycans | Polyethylene glycol | Monoclonal antibodies | Cell differentiation | Sulfates | Polyols | Cells | Antigenic determinants | Binding | Polyethylene | Keratan sulfate | N-acetyllactosamine | Oligosaccharides | Avidin | Substrates | Glycan | Sulfation | Substrate specificity | Rodents | Sulfate | Chemical synthesis | Enzyme-linked immunosorbent assay | Binding sites
Journal Article
Carbohydrate Research, ISSN 0008-6215, 02/2018, Volume 456, pp. 61 - 68
The reaction specificity of the transglycosylation catalyzed by keratanase II from KsT202 (KSase II) was studied by using the oxazoline derivatives having...
Enzymatic glycosylation | Subsite mapping | Keratanase II | Sugar oxazolines | Substrate specificity | Keratan sulfate | CHONDROITIN | CATALYSIS | PROTEOGLYCANS | BIOCHEMISTRY & MOLECULAR BIOLOGY | CHEMISTRY, ORGANIC | ENZYMATIC-SYNTHESIS | OLIGOMERS | BETA-N-ACETYLGLUCOSAMINIDASE | CELL-SURFACE GLYCANS | ORGANIC-SOLVENTS | CHEMISTRY, APPLIED | BINDING | GLYCOSYL HYDROLASES | Carbohydrate Sequence | Carbohydrates - chemistry | Acetylglucosaminidase - metabolism | Substrate Specificity | Glycosylation | Keratan Sulfate - chemistry | Oxazoles - chemistry
Enzymatic glycosylation | Subsite mapping | Keratanase II | Sugar oxazolines | Substrate specificity | Keratan sulfate | CHONDROITIN | CATALYSIS | PROTEOGLYCANS | BIOCHEMISTRY & MOLECULAR BIOLOGY | CHEMISTRY, ORGANIC | ENZYMATIC-SYNTHESIS | OLIGOMERS | BETA-N-ACETYLGLUCOSAMINIDASE | CELL-SURFACE GLYCANS | ORGANIC-SOLVENTS | CHEMISTRY, APPLIED | BINDING | GLYCOSYL HYDROLASES | Carbohydrate Sequence | Carbohydrates - chemistry | Acetylglucosaminidase - metabolism | Substrate Specificity | Glycosylation | Keratan Sulfate - chemistry | Oxazoles - chemistry
Journal Article
Clinical Biochemistry, ISSN 0009-9120, 08/2015, Volume 48, Issue 12, pp. 796 - 802
Morquio A syndrome (mucopolysaccharidosis IVA; MPS IVA) is an autosomal recessive lysosomal storage disorder caused by deficient...
Keratanase | Morquio syndrome | LC/MS–MS | Recombinant enzyme | Mucopolysaccharidosis IV | LC/MS-MS | DOMAIN | PROTEIN | DISACCHARIDES | CELL-SURFACE | ROLES | GROWTH | BACILLUS-ANTHRACIS | MEDICAL LABORATORY TECHNOLOGY | MUCOPOLYSACCHARIDOSIS IVA | Biomarkers - urine | Humans | Child, Preschool | Acetylglucosaminidase - genetics | Case-Control Studies | Bioengineering - methods | Young Adult | Acetylglucosaminidase - metabolism | Cattle | Keratan Sulfate - urine | Cloning, Molecular | Adult | Acetylglucosaminidase - biosynthesis | Catalysis | Child | Tandem Mass Spectrometry - methods | Mucopolysaccharidosis IV - urine | Protein Structure, Tertiary | Escherichia coli - enzymology | Bacillus - genetics | Animals | Bacillus - enzymology | Escherichia coli - genetics | Acetylglucosaminidase - chemistry | Adolescent | Chromatography, Liquid - methods | Measurement | Enzymes | Glycosaminoglycans | Sugars
Keratanase | Morquio syndrome | LC/MS–MS | Recombinant enzyme | Mucopolysaccharidosis IV | LC/MS-MS | DOMAIN | PROTEIN | DISACCHARIDES | CELL-SURFACE | ROLES | GROWTH | BACILLUS-ANTHRACIS | MEDICAL LABORATORY TECHNOLOGY | MUCOPOLYSACCHARIDOSIS IVA | Biomarkers - urine | Humans | Child, Preschool | Acetylglucosaminidase - genetics | Case-Control Studies | Bioengineering - methods | Young Adult | Acetylglucosaminidase - metabolism | Cattle | Keratan Sulfate - urine | Cloning, Molecular | Adult | Acetylglucosaminidase - biosynthesis | Catalysis | Child | Tandem Mass Spectrometry - methods | Mucopolysaccharidosis IV - urine | Protein Structure, Tertiary | Escherichia coli - enzymology | Bacillus - genetics | Animals | Bacillus - enzymology | Escherichia coli - genetics | Acetylglucosaminidase - chemistry | Adolescent | Chromatography, Liquid - methods | Measurement | Enzymes | Glycosaminoglycans | Sugars
Journal Article
ChemBioChem, ISSN 1439-4227, 09/2007, Volume 8, Issue 14, pp. 1710 - 1720
Keratan sulfate (KS) oligomers with well‐defined structures were synthesized by keratanase II (KSase II)‐catalyzed transglycosylation. N‐Acetyllactosamine...
keratan sulfate | oxazolines | ring‐opening polymerization | enzyme catalysis | keratanase II | Ring-opening polymerization | Keratanase II | Enzyme catalysis | Keratan sulfate | Oxazolines | CORNEAL | CHONDROITIN SULFATE | ENDO-BETA-GALACTOSIDASE | IN-VITRO | CHEMISTRY, MEDICINAL | CELL-SURFACE GLYCANS | BIOCHEMISTRY & MOLECULAR BIOLOGY | POLYMERIZATION | PROTEOGLYCAN | CELLULOSE | HYBRID POLYSACCHARIDE | HYALURONIDASE | Carbohydrate Sequence | Models, Chemical | Chromatography - methods | Amino Acids - chemistry | Molecular Sequence Data | Substrate Specificity | Keratan Sulfate - chemistry | Oxazoles - chemistry | Amino Acid Motifs | Hydrolysis | Spectrometry, Mass, Electrospray Ionization | Time Factors | Acetylglucosaminidase - chemistry | Biochemistry - methods | Catalysis | Carbohydrate Conformation | Hydrogen-Ion Concentration
keratan sulfate | oxazolines | ring‐opening polymerization | enzyme catalysis | keratanase II | Ring-opening polymerization | Keratanase II | Enzyme catalysis | Keratan sulfate | Oxazolines | CORNEAL | CHONDROITIN SULFATE | ENDO-BETA-GALACTOSIDASE | IN-VITRO | CHEMISTRY, MEDICINAL | CELL-SURFACE GLYCANS | BIOCHEMISTRY & MOLECULAR BIOLOGY | POLYMERIZATION | PROTEOGLYCAN | CELLULOSE | HYBRID POLYSACCHARIDE | HYALURONIDASE | Carbohydrate Sequence | Models, Chemical | Chromatography - methods | Amino Acids - chemistry | Molecular Sequence Data | Substrate Specificity | Keratan Sulfate - chemistry | Oxazoles - chemistry | Amino Acid Motifs | Hydrolysis | Spectrometry, Mass, Electrospray Ionization | Time Factors | Acetylglucosaminidase - chemistry | Biochemistry - methods | Catalysis | Carbohydrate Conformation | Hydrogen-Ion Concentration
Journal Article
Glycobiology, ISSN 0959-6658, 2001, Volume 11, Issue 10, pp. 779 - 790
Many tissues contain glycoproteins and proteoglycans, which are substituted with N-or O-linked keratan sulfate, a glycosaminoglycan in which the lactosamine...
Keratanases | Glycosaminoglycans | Proteoglycans | Keratan sulfate | Sulfation | glycosaminoglycans | proteoglycans | CHONDROITIN SULFATE | BOVINE ARTICULAR-CARTILAGE | BIOCHEMISTRY & MOLECULAR BIOLOGY | keratanases | NONREDUCING CHAIN TERMINI | FUCOSE RESIDUES | BACTEROIDES-FRAGILIS | ASSISTED CARBOHYDRATE ELECTROPHORESIS | ESCHERICHIA-FREUNDII | keratan sulfate | sulfation | MONOCLONAL-ANTIBODIES | LARGER OLIGOSACCHARIDES | N-ACETYLNEURAMINIC ACID | Carbohydrate Sequence | Chromatography, Ion Exchange | Muscle, Skeletal - enzymology | Cornea - enzymology | Humans | Child, Preschool | Molecular Sequence Data | Chromatography, High Pressure Liquid | Cartilage, Articular - enzymology | Hydrolysis | Animals | Glycoside Hydrolases | Acetylglucosaminidase - metabolism | Cattle | Acetylglucosaminidase - chemistry | Adolescent | beta-Galactosidase - metabolism | Nuclear Magnetic Resonance, Biomolecular | Aged | beta-Galactosidase - chemistry | Electrophoresis - methods
Keratanases | Glycosaminoglycans | Proteoglycans | Keratan sulfate | Sulfation | glycosaminoglycans | proteoglycans | CHONDROITIN SULFATE | BOVINE ARTICULAR-CARTILAGE | BIOCHEMISTRY & MOLECULAR BIOLOGY | keratanases | NONREDUCING CHAIN TERMINI | FUCOSE RESIDUES | BACTEROIDES-FRAGILIS | ASSISTED CARBOHYDRATE ELECTROPHORESIS | ESCHERICHIA-FREUNDII | keratan sulfate | sulfation | MONOCLONAL-ANTIBODIES | LARGER OLIGOSACCHARIDES | N-ACETYLNEURAMINIC ACID | Carbohydrate Sequence | Chromatography, Ion Exchange | Muscle, Skeletal - enzymology | Cornea - enzymology | Humans | Child, Preschool | Molecular Sequence Data | Chromatography, High Pressure Liquid | Cartilage, Articular - enzymology | Hydrolysis | Animals | Glycoside Hydrolases | Acetylglucosaminidase - metabolism | Cattle | Acetylglucosaminidase - chemistry | Adolescent | beta-Galactosidase - metabolism | Nuclear Magnetic Resonance, Biomolecular | Aged | beta-Galactosidase - chemistry | Electrophoresis - methods
Journal Article
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, ISSN 0915-7352, 09/2019, Volume 31, Issue 183, pp. E129 - E136
Keratan sulfate is a sulfated polysaccharide classified as a glycosaminoglycan, which has the structurally unique characteristics of diversity in the linker...
OLIGOSACCHARIDES | II DIGESTION | BIOCHEMISTRY & MOLECULAR BIOLOGY | N-ACETYLLACTOSAMINE | keratanase | ENZYMATIC POLYMERIZATION | SULFOTRANSFERASE | ACETYLGLUCOSAMINE | transglycosylation | CELL-SURFACE GLYCANS | BIOSYNTHESIS | keratan sulfate | sugar oxazolines | MOLECULAR-CLONING | sulfo-sugars
OLIGOSACCHARIDES | II DIGESTION | BIOCHEMISTRY & MOLECULAR BIOLOGY | N-ACETYLLACTOSAMINE | keratanase | ENZYMATIC POLYMERIZATION | SULFOTRANSFERASE | ACETYLGLUCOSAMINE | transglycosylation | CELL-SURFACE GLYCANS | BIOSYNTHESIS | keratan sulfate | sugar oxazolines | MOLECULAR-CLONING | sulfo-sugars
Journal Article
Neuroscience Letters, ISSN 0304-3940, 04/2015, Volume 593, pp. 13 - 18
Functional recovery after neuronal injuries relies on neuronal network reconstruction which involves many repair processes, such as sealing of injured axon...
Chondroitinase ABC | Keratanase II | Chondroitin sulfate | Rehabilitation | Spinal cord injury | Keratan sulfate | FUNCTIONAL RECOVERY | REGENERATION | NEUROSCIENCES | Animals | Spinal Cord Injuries - drug therapy | Neuronal Plasticity | Female | Spinal Cord Injuries - rehabilitation | Spinal Cord Injuries - physiopathology | Chondroitin Sulfates - therapeutic use | Rats, Sprague-Dawley | Keratan Sulfate - therapeutic use | Sulfates | Spinal cord injuries
Chondroitinase ABC | Keratanase II | Chondroitin sulfate | Rehabilitation | Spinal cord injury | Keratan sulfate | FUNCTIONAL RECOVERY | REGENERATION | NEUROSCIENCES | Animals | Spinal Cord Injuries - drug therapy | Neuronal Plasticity | Female | Spinal Cord Injuries - rehabilitation | Spinal Cord Injuries - physiopathology | Chondroitin Sulfates - therapeutic use | Rats, Sprague-Dawley | Keratan Sulfate - therapeutic use | Sulfates | Spinal cord injuries
Journal Article
FEBS JOURNAL, ISSN 1742-464X, 12/2005, Volume 272, Issue 24, pp. 6276 - 6286
Chondroitin and dermatan sulfate (CS and DS) chains were isolated from bovine tracheal cartilage and pig intestinal mucosal preparations and fragmented by...
GLYCOSAMINOGLYCANS | BOVINE ARTICULAR-CARTILAGE | chondroitin sulfate | LINKAGE REGION | BIOCHEMISTRY & MOLECULAR BIOLOGY | HYBRID CHAINS | KERATANASE-II DIGESTION | dermatan sulfate | CAENORHABDITIS-ELEGANS | SPECTROSCOPIC CHARACTERIZATION | BINDING ACTIVITIES | sulfation | HEPARAN-SULFATE | NMR spectroscopy | glycosaminoglycan
GLYCOSAMINOGLYCANS | BOVINE ARTICULAR-CARTILAGE | chondroitin sulfate | LINKAGE REGION | BIOCHEMISTRY & MOLECULAR BIOLOGY | HYBRID CHAINS | KERATANASE-II DIGESTION | dermatan sulfate | CAENORHABDITIS-ELEGANS | SPECTROSCOPIC CHARACTERIZATION | BINDING ACTIVITIES | sulfation | HEPARAN-SULFATE | NMR spectroscopy | glycosaminoglycan
Journal Article
Analytical Biochemistry, ISSN 0003-2697, 1988, Volume 170, Issue 2, pp. 293 - 300
The quantitation of individual glycosaminoglycans in mixtures of polyanions using the dimethylmethylene blue (DMB) method described by R. W. Farndale, D. J....
glycosaminoglycan quantitation | keratanase | dimethylmethylene blue | endo-β- d-galactosidase | keratan sulfates I and II | endo-β-d-galactosidase | Glycosaminoglycans - analysis | Chondroitin Sulfate Proteoglycans - analysis | Electrophoresis, Polyacrylamide Gel | Humans | Structure-Activity Relationship | Methylene Blue - pharmacology | Methylene Blue - analogs & derivatives | Intervertebral Disc - analysis | Pseudomonas - enzymology | beta-Galactosidase - pharmacology | Glycoside Hydrolases | Escherichia - enzymology | Lumican | Galactosidases - pharmacology | Keratan Sulfate - analysis | Cornea - analysis
glycosaminoglycan quantitation | keratanase | dimethylmethylene blue | endo-β- d-galactosidase | keratan sulfates I and II | endo-β-d-galactosidase | Glycosaminoglycans - analysis | Chondroitin Sulfate Proteoglycans - analysis | Electrophoresis, Polyacrylamide Gel | Humans | Structure-Activity Relationship | Methylene Blue - pharmacology | Methylene Blue - analogs & derivatives | Intervertebral Disc - analysis | Pseudomonas - enzymology | beta-Galactosidase - pharmacology | Glycoside Hydrolases | Escherichia - enzymology | Lumican | Galactosidases - pharmacology | Keratan Sulfate - analysis | Cornea - analysis
Journal Article
Glycoconjugate Journal, ISSN 0282-0080, 10/1995, Volume 12, Issue 5, pp. 651 - 659
The repeat region and chain caps of the N-linked keratan sulphates attached to bovine tracheal cartilage fibromodulin were fragmented by digestion with...
small proteoglycan | sulphation | keratan sulphate | fibromodulin | keratanase II | glycosaminoglycan | SULFATE CHAINS | SULFATION | PROTEOGLYCANS | PROTEIN | ACID | FIBROMODULIN | ARTICULAR-CARTILAGE | BIOCHEMISTRY & MOLECULAR BIOLOGY | KERATANASE II | SMALL PROTEOGLYCAN | KERATAN SULFATE | GLYCOSAMINOGLYCAN | Carbohydrate Sequence | Fibromodulin | Chromatography, Ion Exchange | Acetylglucosaminidase | Proteoglycans | Magnetic Resonance Spectroscopy | Molecular Sequence Data | Keratan Sulfate - isolation & purification | Glycosaminoglycans - chemistry | Keratan Sulfate - chemistry | Oligosaccharides - isolation & purification | Glycosaminoglycans - isolation & purification | Animals | Cattle | Extracellular Matrix Proteins | Carrier Proteins - isolation & purification | Oligosaccharides - chemistry | Cartilage - chemistry | Carrier Proteins - chemistry | Carbohydrate Conformation | Trachea | Hydrogen-Ion Concentration | Chain stores
small proteoglycan | sulphation | keratan sulphate | fibromodulin | keratanase II | glycosaminoglycan | SULFATE CHAINS | SULFATION | PROTEOGLYCANS | PROTEIN | ACID | FIBROMODULIN | ARTICULAR-CARTILAGE | BIOCHEMISTRY & MOLECULAR BIOLOGY | KERATANASE II | SMALL PROTEOGLYCAN | KERATAN SULFATE | GLYCOSAMINOGLYCAN | Carbohydrate Sequence | Fibromodulin | Chromatography, Ion Exchange | Acetylglucosaminidase | Proteoglycans | Magnetic Resonance Spectroscopy | Molecular Sequence Data | Keratan Sulfate - isolation & purification | Glycosaminoglycans - chemistry | Keratan Sulfate - chemistry | Oligosaccharides - isolation & purification | Glycosaminoglycans - isolation & purification | Animals | Cattle | Extracellular Matrix Proteins | Carrier Proteins - isolation & purification | Oligosaccharides - chemistry | Cartilage - chemistry | Carrier Proteins - chemistry | Carbohydrate Conformation | Trachea | Hydrogen-Ion Concentration | Chain stores
Journal Article
Carbohydrate Research, ISSN 0008-6215, 2003, Volume 338, Issue 10, pp. 1133 - 1138
Highly sulphated keratan di- and tetrasaccharides were prepared from keratan sulphate (KS) of shark cartilage by enzymatic digestion with keratanase II and...
Desulphation | Keratanase II | Methylation analysis | Oligosaccharide | Keratan sulphate | ENDO-BETA-GALACTOSIDASE | PROTEOGLYCANS | keratan sulphate | OLIGOSACCHARIDES | CUCUMBER STICHOPUS-JAPONICUS | BIOCHEMISTRY & MOLECULAR BIOLOGY | CHEMISTRY, ORGANIC | oligosaccharide | keratanase II | desulphation | HEPARAN-SULFATE | DISACCHARIDE | CHEMISTRY, APPLIED | methylation analysis | Sulfates - metabolism | Keratan Sulfate - metabolism | Sharks | Oligosaccharides - metabolism | Keratan Sulfate - chemistry | Animals | Time Factors | Acetylglucosaminidase - metabolism | Oligosaccharides - chemistry | Cartilage - chemistry | Hydrochloric Acid - chemistry | Cartilage - enzymology | Methanol - chemistry | Hydrogen-Ion Concentration
Desulphation | Keratanase II | Methylation analysis | Oligosaccharide | Keratan sulphate | ENDO-BETA-GALACTOSIDASE | PROTEOGLYCANS | keratan sulphate | OLIGOSACCHARIDES | CUCUMBER STICHOPUS-JAPONICUS | BIOCHEMISTRY & MOLECULAR BIOLOGY | CHEMISTRY, ORGANIC | oligosaccharide | keratanase II | desulphation | HEPARAN-SULFATE | DISACCHARIDE | CHEMISTRY, APPLIED | methylation analysis | Sulfates - metabolism | Keratan Sulfate - metabolism | Sharks | Oligosaccharides - metabolism | Keratan Sulfate - chemistry | Animals | Time Factors | Acetylglucosaminidase - metabolism | Oligosaccharides - chemistry | Cartilage - chemistry | Hydrochloric Acid - chemistry | Cartilage - enzymology | Methanol - chemistry | Hydrogen-Ion Concentration
Journal Article
Glycobiology, ISSN 0959-6658, 1997, Volume 7, Issue 3, pp. 405 - 412
We have previously shown that chondroitin 6-sulfotransferase (C6ST) catalyzes transfer of sulfate not only to position 6 of GalNAc residue of chondroitin but...
L-selectin | Sialyl lactosamine | Sialyl Lewis x | Keratan sulfate | Sulfotransferase | sialyl Lewis x | ENDOTHELIAL-DERIVED LIGAND | GLYCAM-1 | MOLECULE | BIOCHEMISTRY & MOLECULAR BIOLOGY | sulfotransferase | CHROMATOGRAPHY | IDENTIFICATION | KERATANASE-II DIGESTION | CARTILAGE | K CQ BIOCHEMISTRY & MOLECULAR BIOLOGY | LEWIS-X | PURIFICATION | keratan sulfate | sialyl lactosamine | Carbohydrate Sequence | Recombinant Proteins - metabolism | Animals | Sulfates - metabolism | Borohydrides - pharmacology | Chickens | beta-Galactosidase - metabolism | Molecular Sequence Data | Neuraminidase - metabolism | Sulfotransferases - metabolism | Chromatography, High Pressure Liquid | Oligosaccharides - metabolism
L-selectin | Sialyl lactosamine | Sialyl Lewis x | Keratan sulfate | Sulfotransferase | sialyl Lewis x | ENDOTHELIAL-DERIVED LIGAND | GLYCAM-1 | MOLECULE | BIOCHEMISTRY & MOLECULAR BIOLOGY | sulfotransferase | CHROMATOGRAPHY | IDENTIFICATION | KERATANASE-II DIGESTION | CARTILAGE | K CQ BIOCHEMISTRY & MOLECULAR BIOLOGY | LEWIS-X | PURIFICATION | keratan sulfate | sialyl lactosamine | Carbohydrate Sequence | Recombinant Proteins - metabolism | Animals | Sulfates - metabolism | Borohydrides - pharmacology | Chickens | beta-Galactosidase - metabolism | Molecular Sequence Data | Neuraminidase - metabolism | Sulfotransferases - metabolism | Chromatography, High Pressure Liquid | Oligosaccharides - metabolism
Journal Article
Glycoconjugate Journal, ISSN 0282-0080, 7/1997, Volume 14, Issue 5, pp. 651 - 660
The small keratan sulphate proteoglycan, fibromodulin, has been isolated from pooled human articular cartilage. The main chain repeat region and the chain caps...
Life Sciences | Biochemistry, general | small proteoglycan | Pathology | sulphation | keratan sulphate | fibromodulin | keratanase II | glycosaminoglycan | human | Fibromodulin | Human | Keratan sulphate | Sulphation | Glycosaminoglycan | Keratanase II | Small proteoglycan | PROTEOGLYCANS | COLLAGEN | OLIGOSACCHARIDES | II DIGESTION | BIOCHEMISTRY & MOLECULAR BIOLOGY | IDENTIFICATION | CHROMATOGRAPHY | SULFATE CHAINS | K CQ BIOCHEMISTRY & MOLECULAR BIOLOGY | CARBOHYDRATE DIFFERENTIATION ANTIGENS | BOVINE TRACHEAL CARTILAGE | N-ACETYLNEURAMINIC ACID | Carbohydrate Sequence | Chromatography, Ion Exchange | Femur | Acetylglucosaminidase | Proteoglycans | Species Specificity | Humans | Molecular Sequence Data | Femur Head | Keratan Sulfate - chemistry | Animals | Cattle | Extracellular Matrix Proteins | Oligosaccharides - chemistry | Horses | Carrier Proteins - chemistry | N-Acetylneuraminic Acid - analysis | Carbohydrate Conformation | Chromatography, Gel | Cartilage, Articular - chemistry | Chain stores | Cartilage | Nuclear magnetic resonance--NMR
Life Sciences | Biochemistry, general | small proteoglycan | Pathology | sulphation | keratan sulphate | fibromodulin | keratanase II | glycosaminoglycan | human | Fibromodulin | Human | Keratan sulphate | Sulphation | Glycosaminoglycan | Keratanase II | Small proteoglycan | PROTEOGLYCANS | COLLAGEN | OLIGOSACCHARIDES | II DIGESTION | BIOCHEMISTRY & MOLECULAR BIOLOGY | IDENTIFICATION | CHROMATOGRAPHY | SULFATE CHAINS | K CQ BIOCHEMISTRY & MOLECULAR BIOLOGY | CARBOHYDRATE DIFFERENTIATION ANTIGENS | BOVINE TRACHEAL CARTILAGE | N-ACETYLNEURAMINIC ACID | Carbohydrate Sequence | Chromatography, Ion Exchange | Femur | Acetylglucosaminidase | Proteoglycans | Species Specificity | Humans | Molecular Sequence Data | Femur Head | Keratan Sulfate - chemistry | Animals | Cattle | Extracellular Matrix Proteins | Oligosaccharides - chemistry | Horses | Carrier Proteins - chemistry | N-Acetylneuraminic Acid - analysis | Carbohydrate Conformation | Chromatography, Gel | Cartilage, Articular - chemistry | Chain stores | Cartilage | Nuclear magnetic resonance--NMR
Journal Article
Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology, ISSN 1073-6085, 1996, Volume 6, Issue 3, pp. 241 - 265
NMR spectroscopy has played a developing role in the study of polysaccharide structures for over 30 years. Many new bacterial polysaccharide repeat unit...
Polysaccharides: lipopolysaccharides: bacterial polysaccharides | Glycosaminoglycans | Polysaccharide conformation | Nuclear magnetic resonance (NMR) | Plant polysaccharides | polysaccharides | glycosaminoglycans | O-SPECIFIC POLYSACCHARIDE | lipopolysaccharides | bacterial polysaccharides | BOVINE ARTICULAR-CARTILAGE | BIOCHEMISTRY & MOLECULAR BIOLOGY | NUCLEAR-MAGNETIC-RESONANCE | CELL-WALL POLYSACCHARIDES | ESCHERICHIA-COLI O6-K54-H10 | KERATANASE-II DIGESTION | PORCINE INTESTINAL HEPARIN | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | nuclear magnetic resonance (NMR) | CHEMICALLY-MODIFIED HEPARINS | polysaccharide conformation | C-13-H-1 COUPLING-CONSTANTS | N-ACETYLNEURAMINIC ACID | plant polysaccharides | Carbohydrate Sequence | Protons | Animals | Magnetic Resonance Spectroscopy | Molecular Sequence Data | Polysaccharides - chemistry | Molecular Structure | Carbon
Polysaccharides: lipopolysaccharides: bacterial polysaccharides | Glycosaminoglycans | Polysaccharide conformation | Nuclear magnetic resonance (NMR) | Plant polysaccharides | polysaccharides | glycosaminoglycans | O-SPECIFIC POLYSACCHARIDE | lipopolysaccharides | bacterial polysaccharides | BOVINE ARTICULAR-CARTILAGE | BIOCHEMISTRY & MOLECULAR BIOLOGY | NUCLEAR-MAGNETIC-RESONANCE | CELL-WALL POLYSACCHARIDES | ESCHERICHIA-COLI O6-K54-H10 | KERATANASE-II DIGESTION | PORCINE INTESTINAL HEPARIN | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | nuclear magnetic resonance (NMR) | CHEMICALLY-MODIFIED HEPARINS | polysaccharide conformation | C-13-H-1 COUPLING-CONSTANTS | N-ACETYLNEURAMINIC ACID | plant polysaccharides | Carbohydrate Sequence | Protons | Animals | Magnetic Resonance Spectroscopy | Molecular Sequence Data | Polysaccharides - chemistry | Molecular Structure | Carbon
Journal Article
Polymer Preprints, Japan, 2005, Volume 54, Issue 1, p. 2156
Conference Proceeding
EUROPEAN JOURNAL OF BIOCHEMISTRY, ISSN 0014-2956, 03/2001, Volume 268, Issue 5, pp. 1181 - 1189
Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and chondroitin ACII lyase; both disaccharide and tetrasaccharide...
PROTEOGLYCANS | BOVINE ARTICULAR-CARTILAGE | NMR-SPECTROSCOPY | LINKAGE REGION | BIOCHEMISTRY & MOLECULAR BIOLOGY | KERATANASE-II DIGESTION | ADHESION | GLUCURONIC-ACID | sulfation | HEPARAN-SULFATE | NMR spectroscopy | ANTICOAGULANT ACTION | glycosaminoglycan | ASSIGNMENTS
PROTEOGLYCANS | BOVINE ARTICULAR-CARTILAGE | NMR-SPECTROSCOPY | LINKAGE REGION | BIOCHEMISTRY & MOLECULAR BIOLOGY | KERATANASE-II DIGESTION | ADHESION | GLUCURONIC-ACID | sulfation | HEPARAN-SULFATE | NMR spectroscopy | ANTICOAGULANT ACTION | glycosaminoglycan | ASSIGNMENTS
Journal Article
Journal of Biological Chemistry, ISSN 0021-9258, 07/2003, Volume 278, Issue 28, pp. 25766 - 25772
Keratan sulfate (KS) is degraded by various enzymes including endo-beta-galactosidase, keratanase, and keratanase II, which are used for the structural...
LIGANDS | BIOCHEMISTRY & MOLECULAR BIOLOGY | E-SELECTIN | CELL-ADHESION | DEFICIENT MICE | SIALYL-LEX | PROTEOGLYCAN | PEANUT AGGLUTININ | GALACTOSIDASE | Temperature | Sharks | Amino Acids - chemistry | Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - biosynthesis | Molecular Sequence Data | Substrate Specificity | Chromatography, High Pressure Liquid | Electrophoresis, Capillary | Time Factors | Cattle | Oligosaccharides - chemistry | Cloning, Molecular | Protein Denaturation | beta-Galactosidase - metabolism | Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - genetics | Protein Structure, Tertiary | Peptides - chemistry | Electrophoresis, Polyacrylamide Gel | Carbohydrate Metabolism | Hyaluronic Acid - chemistry | Keratan Sulfate - metabolism | Chondroitin Sulfates - chemistry | Cartilage - metabolism | Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - chemistry | Chromatography | Hydrolysis | Animals | Glycoside Hydrolases | Bacillus - enzymology | Heparitin Sulfate - chemistry | Hydrogen-Ion Concentration | Keratan sulfate | keratanase | Endo- beta -N-acetylglucosaminidase
LIGANDS | BIOCHEMISTRY & MOLECULAR BIOLOGY | E-SELECTIN | CELL-ADHESION | DEFICIENT MICE | SIALYL-LEX | PROTEOGLYCAN | PEANUT AGGLUTININ | GALACTOSIDASE | Temperature | Sharks | Amino Acids - chemistry | Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - biosynthesis | Molecular Sequence Data | Substrate Specificity | Chromatography, High Pressure Liquid | Electrophoresis, Capillary | Time Factors | Cattle | Oligosaccharides - chemistry | Cloning, Molecular | Protein Denaturation | beta-Galactosidase - metabolism | Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - genetics | Protein Structure, Tertiary | Peptides - chemistry | Electrophoresis, Polyacrylamide Gel | Carbohydrate Metabolism | Hyaluronic Acid - chemistry | Keratan Sulfate - metabolism | Chondroitin Sulfates - chemistry | Cartilage - metabolism | Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - chemistry | Chromatography | Hydrolysis | Animals | Glycoside Hydrolases | Bacillus - enzymology | Heparitin Sulfate - chemistry | Hydrogen-Ion Concentration | Keratan sulfate | keratanase | Endo- beta -N-acetylglucosaminidase
Journal Article
Histochemical Journal, ISSN 0018-2214, 1996, Volume 28, Issue 5, pp. 341 - 351
The type and distribution of mineral binding and collagenous matrix-associated chondroitin sulphate and dermatan sulphate proteoglycans in rabbit alveolar bone...
D-GALACTOSIDASE KERATANASE | MINERAL-BINDING PROTEOGLYCANS | BOVINE BONE | DEMINERALIZATION | SULFATE PROTEOGLYCAN | MONOCLONAL-ANTIBODY | MATRIX PROTEINS | CALCIFYING CONNECTIVE TISSUES | SIALOPROTEIN-II | COLLAGEN FIBRIL INTERACTIONS | CELL BIOLOGY | Immunohistochemistry | Rabbits | Animals | Chondroitin Sulfates - metabolism | Proteoglycans - metabolism | Chromatography, Gel | Mandible - ultrastructure | Blotting, Western | Mandible - metabolism
D-GALACTOSIDASE KERATANASE | MINERAL-BINDING PROTEOGLYCANS | BOVINE BONE | DEMINERALIZATION | SULFATE PROTEOGLYCAN | MONOCLONAL-ANTIBODY | MATRIX PROTEINS | CALCIFYING CONNECTIVE TISSUES | SIALOPROTEIN-II | COLLAGEN FIBRIL INTERACTIONS | CELL BIOLOGY | Immunohistochemistry | Rabbits | Animals | Chondroitin Sulfates - metabolism | Proteoglycans - metabolism | Chromatography, Gel | Mandible - ultrastructure | Blotting, Western | Mandible - metabolism
Journal Article