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1. Limiting dilution analysis of cells in the immune system
by Lefkovits, Ivan and Waldmann, Hermann
1979, ISBN 0521227712, 262
Plaque assays technique | Statistical methods | Immunology | B cells | T cells
Book
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2. Full Text Limiting dilution analysis of murine epidermal stem cells using an in vivo regeneration assay
by Strachan, Lauren R and Ghadially, Ruby
Methods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2010, Volume 585, pp. 421 - 432
Epidermal stem cells are of major importance for tissue homeostasis, wound repair, tumor initiation, and gene therapy. Here we describe an in vivo regeneration... 
Cell Lineage | Regeneration | Animals | Cell Proliferation | Epidermis - physiology | Cell Differentiation | Keratinocytes - cytology | Mice | Stem Cells - cytology | Cell Culture Techniques | Mice, SCID | Epidermis - cytology | skin | transit amplifying cell | limiting dilution | regeneration | Stem cell | epidermis
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3. Full Text Clonal analysis reveals multiple functional defects of aged murine hematopoietic stem cells
by Dykstra, Brad and Olthof, Sana and Schreuder, Jaring and Ritsema, Martha and de Haan, Gerald
Journal of Experimental Medicine, ISSN 0022-1007, 12/2011, Volume 208, Issue 13, pp. 2691 - 2703
Hematopoietic stem cell (HSC) populations change with aging, but the extent to which this is caused by qualitative versus quantitative alterations in HSC... 
HETEROGENEITY | LIFE-SPAN | MECHANISM | IN-VIVO | MOUSE | SELF-RENEWAL | DIFFERENTIATION | COMPARTMENT | FREQUENCY-ANALYSIS | UMCG Approved | LIMITING DILUTION ANALYSIS | MEDICINE, RESEARCH & EXPERIMENTAL | IMMUNOLOGY | Animals | Cell Proliferation | Cellular Senescence | Hematopoietic Stem Cells - pathology | Antigens, Differentiation - metabolism | Mice, Transgenic | Hematopoietic Stem Cells - metabolism | Mice | Hematopoietic Stem Cell Transplantation - methods | Transplantation, Homologous | Colony-Forming Units Assay - methods | 308
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4. Full Text A strategy for selective, CD4 + T cell-independent activation of virus-specific memory B cells for limiting dilution analysis
by Li, Xiaofeng and Vanitha, Daisy J and Joo, Hye Mee and He, Yuxia and Rouse, Barry T and Sangster, Mark Y
Journal of Immunological Methods, ISSN 0022-1759, 2006, Volume 313, Issue 1, pp. 110 - 118
Complete characterization of the B cell response to infection or vaccination is dependent on accurate quantitation of the memory B cell (MBC) pool. An... 
Memory B cell | Influenza virus | Limiting dilution assay | Herpes simplex virus | HELP | limiting dilution assay | memory B cell | BIOCHEMICAL RESEARCH METHODS | IMMUNOLOGY | PLASMA-CELLS | herpes simplex virus | IMMUNIZATION | HERPES-SIMPLEX-VIRUS | REPLICATION | LYMPHOID-TISSUE | influenza virus | INFECTION | MICE | DEPENDENT DIFFERENTIATION | ANTIGEN | Spleen - virology | Spleen - immunology | Cell Count | Coculture Techniques | Humans | Cercopithecus aethiops | Lymphoid Tissue - immunology | CD4-Positive T-Lymphocytes - immunology | Lymphocyte Activation - immunology | Female | Herpesvirus 1, Human - immunology | Vero Cells | Immunologic Memory - immunology | B-Lymphocytes - cytology | Mice, Inbred C57BL | Viruses - immunology | Spleen - cytology | Influenza A Virus, H3N2 Subtype - immunology | Herpes Simplex - immunology | Lymphoid Tissue - cytology | Animals | B-Lymphocytes - immunology | Influenza A Virus, H3N2 Subtype - chemistry | Viruses - chemistry | Mice | Orthomyxoviridae Infections - immunology
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5. Full Text Hematopoietic Stem Cell Subtypes Expand Differentially during Development and Display Distinct Lymphopoietic Programs
by Benz, Claudia and Copley, Michael R and Kent, David G and Wohrer, Stefan and Cortes, Adrian and Aghaeepour, Nima and Ma, Elaine and Mader, Heidi and Rowe, Keegan and Day, Christopher and Treloar, David and Brinkman, Ryan R and Eaves, Connie J
Cell Stem Cell, ISSN 1934-5909, 03/2012, Volume 10, Issue 3, pp. 273 - 283
Adult hematopoietic stem cells (HSCs) with serially transplantable activity comprise two subtypes. One shows a balanced output of mature lymphoid and myeloid... 
HETEROGENEITY | B-LYMPHOPOIESIS | IN-VIVO | REPOPULATION | POPULATIONS | AGED MICE | TERM | COMPARTMENT | LIMITING DILUTION ANALYSIS | CLONAL ANALYSIS | CELL & TISSUE ENGINEERING | CELL BIOLOGY | Pregnancy | Cell Lineage | Lymphopoiesis | Animals | Flow Cytometry | Cellular Senescence | Hematopoietic Stem Cells - cytology | Humans | Mice, Inbred C57BL | Female | Cell Differentiation | Mice
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6. Full Text Limiting dilution analysis of interleukin-2 producing helper T-cell frequencies as a tool in allogeneic hematopoietic cell transplantation
by Petersen, Søren L and Sidorov, Igor A and Russell, Charlotte A and Dickmeiss, Ebbe and Vindeløv, Lars L
Transplantation, ISSN 0041-1337, 09/2005, Volume 80, Issue 5, pp. 573 - 581
Background. A reliable in vitro test that estimates the level of ongoing alloreactivity would be valuable in allogeneic hematopoietic cell transplantation... 
Graft-versus-host disease | Nonmyeloablative conditioning regimen | Hematopoietic cell transplantation | Regulatory cells | Limiting dilution analysis | SURGERY | BONE-MARROW-TRANSPLANTATION | ELIMINATION | PREDICTIVE VALUE | IMMUNOLOGY | VERSUS-HOST-DISEASE | PRECURSOR FREQUENCY | regulatory cells | TRANSPLANTATION | IN-VITRO | hematopoietic cell transplantation | limiting dilution analysis | HLA-IDENTICAL SIBLINGS | MALIGNANCIES | OPTIMIZATION | graft-versus-host disease | nonmyeloablative conditioning regimen | BLOOD | Hematologic Neoplasms - therapy | Interleukin-2 - metabolism | T-Lymphocytes, Helper-Inducer - metabolism | Humans | Graft vs Tumor Effect - immunology | Bone Marrow Transplantation - methods | Graft vs Host Disease - immunology | Transplantation, Homologous | Bone Marrow Transplantation - immunology | Models, Immunological | Feedback, Physiological - immunology | T-Lymphocytes, Helper-Inducer - immunology | Immunoassay - methods | Graft vs Host Disease - prevention & control | Indicator Dilution Techniques | In Vitro Techniques
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7. Full Text Mechanisms that determine plasma cell lifespan and the duration of humoral immunity
by Amanna, Ian J and Slifka, Mark K
Immunological Reviews, ISSN 0105-2896, 07/2010, Volume 236, Issue 1, pp. 125 - 138
Humoral immunity following vaccination or infection is mainly derived from two types of cells: memory B cells and plasma cells. Memory B cells do not actively... 
antibody | plasma cell | immunological memory | memory B cell | Memory B cell | Immunological memory | Antibody | Plasma cell | BONE-MARROW | LYMPH NODE CELLS | IMMUNOLOGY | ANTIBODY-PRODUCING CELLS | LIMITING DILUTION ANALYSIS | VIRAL-INFECTION | MEMORY B-CELLS | FOLLICULAR DENDRITIC CELLS | SMALLPOX VACCINATION | GERMINAL-CENTERS | T-CELLS | Models, Immunological | Animals | Time Factors | Immunity, Humoral - immunology | Humans | Vaccination | Antibodies - immunology | Antigens - immunology | Cell Survival - immunology | Plasma Cells - immunology | Immunoglobulins | Analysis
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8. Full Text Limiting dilution analysis of virus-specific memory B cells by an ELISPOT assay
by Slifka, Mark K and Ahmed, Rafi
Journal of Immunological Methods, ISSN 0022-1759, 1996, Volume 199, Issue 1, pp. 37 - 46
Many acute viral infections induce long-term, sometimes even life-long humoral immunity. To characterize this immune response, accurate quantitation of memory... 
Memory B cell | Lymphocytic choriomeningitis virus | Limiting dilution analysis | ELISPOT | ELISA | IMMUNE-RESPONSE | CLEARANCE | ACTIVATION | BONE-MARROW | BIOCHEMICAL RESEARCH METHODS | T-CELL | IMMUNOLOGY | SPLEENS | VIRAL-INFECTION | ANTIGEN-SPECIFIC MEMORY | INVIVO | limiting dilution analysis | lymphocytic choriomeningitis virus | Enzyme-Linked Immunosorbent Assay - methods | Lymphocytic choriomeningitis virus - immunology | Antibody Specificity | Animals | B-Lymphocytes - immunology | Cell Separation | Cell Count | Antibodies, Viral - chemistry | Immunologic Memory | Mice | Mice, Inbred BALB C
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9. Full Text Irradiation of platelet components: inhibition of lymphocyte proliferation assessed by limiting‐dilution analysis
by Luban, N.L.C and Drothler, D and Moroff, G and Quinones, R
Transfusion, ISSN 0041-1132, 03/2000, Volume 40, Issue 3, pp. 348 - 352
BACKGROUND: Using a limiting‐dilution analysis (LDA) assay that measures clonigenic T cells, it has been demonstrated that, with 2500 cGy, there is no T‐cell... 
LDA = limiting‐dilution analysis | WBC(s) = white cell(s) | RBC(s) = red cell(s) | TA‐GVHD = transfusion‐associated graft‐versus‐host disease | IL = interleukin | MNC(s) = mononuclear cell(s) | STORAGE | GAMMA-IRRADIATION | BONE-MARROW TRANSPLANTATION | MEDIATED GRAFT-REJECTION | HEMATOLOGY | RADIATION | VERSUS-HOST DISEASE | BLOOD COMPONENTS | Blood Platelets - radiation effects | CD8-Positive T-Lymphocytes - cytology | Gamma Rays | Lymphocyte Activation | CD4-Positive T-Lymphocytes - cytology | CD56 Antigen - analysis | Cell Count | Humans | CD4-CD8 Ratio | T-Lymphocytes - cytology | Plateletpheresis | Cell Division | Adult | Leukocytes, Mononuclear - cytology | T-Lymphocytes - immunology | Indicator Dilution Techniques | Dose-Response Relationship, Radiation
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10. CD31(-) HipOps - A Highly Osteogenic Cell Population From Mouse Bone Marrow
by McKenzie, Kristen Penny
11/2012
Multipotent mesenchymal stem cells (MSCs), found in many adult tissues, may be useful for regenerative medicine applications. Their identification and... 
CD31 | mesenchymal stem cell | CD73 | cell marker | bone marrow stromal cell | BMSC | cell sorting | negative selection | MACS | osteoblast | osteogenesis | accessory population | bone marrow | highly purified osteoprogenitor population | osteoprogenitor | magnetic bead cell sorting | platelet endothelial cell adhesion molecule | 0379 | cell culture | limiting dilution analysis | HipOp | PECAM | vascular endothelial cell
Dissertation
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11. Full Text Transfusion‐associated graft‐versus‐host disease reexamined: potential for improved prevention using a universally applied intervention
by Kleinman, Steven and Stassinopoulos, Adonis
Transfusion, ISSN 0041-1132, 11/2018, Volume 58, Issue 11, pp. 2545 - 2563
BACKGROUND Transfusion‐associated graft‐versus‐host disease (TA‐GVHD) is a rare, often fatal complication of blood transfusion that can occur in... 
WHITE CELL-REDUCTION | GAMMA-IRRADIATION | PATHOGEN REDUCTION SYSTEM | CARDIAC-SURGERY | PHOTOCHEMICAL TREATMENT | RED-CELLS | OF-THE-LITERATURE | HUMAN-IMMUNODEFICIENCY-VIRUS | LIMITING-DILUTION ANALYSIS | HEMATOLOGY | BLOOD COMPONENTS | T cells | Political corruption | Blood transfusion | Analysis | Cell proliferation | Graft-versus-host reaction | Experimental data | Deactivation | Transfusion | Case reports | Lymphocytes T | Inactivation | Blood | Radiation dosage | Gamma rays | Gamma irradiation | Dilution | Cell growth | Lymphocytes | Irradiation | Immunocompromised hosts | Inhibition | Glutathione
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12. Full Text Amotosalen/UVA treatment inactivates T cells more effectively than the recommended gamma dose for prevention of transfusion‐associated graft‐versus‐host disease
by Castro, Grace and Merkel, Patricia A and Giclas, Hannah E and Gibula, Andrew and Andersen, Gillian E and Corash, Laurence M and Lin, Jin Sying and Green, Jennifer and Knight, Vijaya and Stassinopoulos, Adonis
Transfusion, ISSN 0041-1132, 06/2018, Volume 58, Issue 6, pp. 1506 - 1515
INTRODUCTION Transfusion‐associated graft‐versus‐host disease (TA‐GVHD) is a rare complication after transfusion of components containing viable donor T cells.... 
IRRADIATION | LEUKOCYTES | REDUCTION | PHOTOCHEMICAL TREATMENT | GUIDELINES | LIGHT | ULTRAVIOLET | LIMITING-DILUTION ANALYSIS | HEMATOLOGY | RADIATION | BLOOD COMPONENTS | Gamma Rays | Lymphocyte Activation - radiation effects | Furocoumarins - pharmacology | Humans | T-Lymphocytes - radiation effects | Treatment Outcome | Transfusion Reaction - prevention & control | Furocoumarins - therapeutic use | Blood Transfusion | Ultraviolet Rays | T-Lymphocytes - cytology | Lymphocyte Activation - drug effects | T-Lymphocytes - drug effects | Graft vs Host Disease - prevention & control | Cell Proliferation - radiation effects | T cells | Political corruption | Blood transfusion | Analysis | Cell proliferation | Cell culture | Graft-versus-host reaction | Transfusion | Deactivation | U.V. radiation | Blood cells | Erythrocytes | Leukocytes (mononuclear) | Lymphocytes T | Thymidine | Kinases | Inactivation | Blood | Prevention | White blood cells | Gamma rays | Gamma irradiation | Dilution | Cell growth | Lymphocytes | Peripheral blood mononuclear cells | Irradiation | Food irradiation
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13. Full Text Analytical assessment of clonal derivation of eukaryotic/CHO cell populations
by Aebischer-Gumy, Christel and Moretti, Pierre and Little, Thomas A and Bertschinger, Martin
Journal of Biotechnology, ISSN 0168-1656, 11/2018, Volume 286, pp. 17 - 26
Cell populations that are exclusively composed by descendants from a defined single parental progenitor are referred to as “monoclonal”, “clonal” or more... 
Homogeneity | Clonality | Statistical analysis | Cell line development | Monoclonality | Clonal derivation | MAMMALIAN-CELLS | VIEW | LIMITING DILUTION ANALYSIS | LINES | STATISTICAL-ANALYSIS | IMMUNE-SYSTEM | CLONING | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | Clone Cells - cytology | Animals | Cricetulus | Plasmids - genetics | Polymerase Chain Reaction | High-Throughput Nucleotide Sequencing - methods | Transgenes | Sequence Analysis, DNA - methods | CHO Cells | Genomics | Analysis
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14. Full Text A stochastic model for cancer stem cell origin in metastatic colon cancer
by Odoux, Christine and Fohrer, Helene and Hoppo, Toshitaka and Guzik, Lynda and Stolz, Donna Beer and Lewis, Dale W and Gollin, Susanne M and Gamblin, T. Clark and Geller, David A and Lagasse, Eric
Cancer Research, ISSN 0008-5472, 09/2008, Volume 68, Issue 17, pp. 6932 - 6941
Human cancers have been found to include transformed stem cells that may drive cancer progression to metastasis. Here, we report that metastatic colon cancer... 
INITIATING CELLS | GENETIC INSTABILITY | ONCOLOGY | COLORECTAL-CANCER | MOUSE | IDENTIFICATION | MULTILINEAGE DIFFERENTIATION | CULTURE | TUMORIGENESIS | LIMITING DILUTION ANALYSIS | CHRONIC MYELOID-LEUKEMIA | Microscopy, Electron, Transmission | Neoplastic Stem Cells - cytology | Humans | Middle Aged | Male | Mice, Knockout | Neoplasm Metastasis | Animals | Stochastic Processes | Aged, 80 and over | Female | Aged | Mice | Colorectal Neoplasms - pathology | Chromosomal Instability in Cancer Stem Cells
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15. Full Text High‐Throughput, Miniaturized Clonogenic Analysis of a Limiting Dilution Assay on a Micropillar/Microwell Chip with Brain Tumor Cells
by Lee, Dong Woo and Choi, Yeon‐Sook and Seo, Yun Jee and Lee, Moo‐Yeal and Jeon, Sang Youl and Ku, Bosung and Nam, Do‐Hyun
Small, ISSN 1613-6810, 12/2014, Volume 10, Issue 24, pp. 5098 - 5105
The limiting dilution assay (LDA) is a clonogenic drug efficacy test designed to determine a value for drug efficacy based on an all‐or‐none (positive or... 
micropillar/microwell chips | tumor cells | clonogenic assays | high throughput screening | limiting dilution assays | SURVIVAL | PHYSICS, CONDENSED MATTER | STEM-CELLS | PHYSICS, APPLIED | MATERIALS SCIENCE, MULTIDISCIPLINARY | CHEMISTRY, PHYSICAL | NANOSCIENCE & NANOTECHNOLOGY | CULTURE | CHEMISTRY, MULTIDISCIPLINARY | BLOOD | High-Throughput Screening Assays | Miniaturization | Glioblastoma - pathology | Humans | Brain Neoplasms - pathology | Gliomas | Analysis | Brain tumors | Dilution | Drugs | Inhibitors | Effectiveness | Colonies | Chips | Assaying | Constraining | Tumors
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16. Full Text Immunological memories of the bone marrow
by Chang, Hyun‐Dong and Tokoyoda, Koji and Radbruch, Andreas
Immunological Reviews, ISSN 0105-2896, 05/2018, Volume 283, Issue 1, pp. 86 - 98
Summary Memory for antigens once encountered is a hallmark of the immune system of vertebrates, providing us with an immunity adapted to pathogens of our... 
bone marrow | memory T cells | tissue‐resident memory | tissue-resident memory | DENDRITIC CELLS | IMMUNOLOGY | HOMEOSTATIC PROLIFERATION | CD8(+) T-CELLS | LIMITING DILUTION ANALYSIS | HELPER LYMPHOCYTES | CUTTING EDGE | B-CELLS | SEROLOGICAL MEMORY | LIVED PLASMA-CELLS | SECRETING CELLS | T-Lymphocyte Subsets - immunology | Bone Marrow - immunology | Humans | Homeostasis | Cell Survival - immunology | Organ Specificity - immunology | Lymphocyte Activation - immunology | Animals | T-Lymphocyte Subsets - metabolism | Bone Marrow - metabolism | Bone Marrow Cells - immunology | Immunologic Memory | Plasma Cells - metabolism | Bone Marrow Cells - metabolism | Plasma Cells - immunology | T cells | Antigens | Immunological memory | Mesenchyme | Tissues | Immunity | Vertebrates | Immunology | Lymphocytes | Stromal cells | Bone marrow | Plasma cells | Niches | Immune system | Invited Reviews | Invited Review
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