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1. Full Text Nanoparticles Reveal That Human Cervicovaginal Mucus Is Riddled with Pores Larger than Viruses
by Samuel K. Lai and Ying-Ying Wang and Kaoru Hida and Richard Cone and Justin Hanes and Robert Langer
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 1/2010, Volume 107, Issue 2, pp. 598 - 603
The mechanisms by which mucus helps prevent viruses from infecting mucosal surfaces are not well understood. We engineered non-mucoadhesive nanoparticles of... 
Nanoparticles | Bacterial vaginosis | Particle diffusion | Adhesives | Gels | Particle interactions | Mucins | Viruses | Mucus | Cervical mucus | Hydrophobic interactions | Microstructure | Cervicovaginal tract | Particle tracking | Herpes simplex virus | MULTIPLE-PARTICLE TRACKING | GASTRIC MUCIN | microstructure | MULTIDISCIPLINARY SCIENCES | GLYCOPROTEINS | particle tracking | AIRWAY MUCUS | BACTERIAL VAGINOSIS | herpes simplex virus | HERPES-SIMPLEX-VIRUS | HUMAN CERVICAL-MUCUS | hydrophobic interactions | VAGINAL MICROBICIDES | DIFFUSION | CYSTIC-FIBROSIS | cervicovaginal tract | Viscosity | Simplexvirus - physiology | Polyethylene Glycols | Cervix Mucus - virology | Humans | Mucus - virology | Elasticity | Simplexvirus - ultrastructure | Cervix Uteri - ultrastructure | Vagina - ultrastructure | Cell Adhesion | Ovulation | Mucins - ultrastructure | Biological Transport | Cervix Uteri - physiology | Vagina - physiology | Female | Cervix Mucus - physiology | Physiological aspects | Usage | Research | Mucous membrane | Body fluids | Lipids | Reproductive system | Index Medicus | Biological Sciences
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2. Full Text Rapid Transport of Large Polymeric Nanoparticles in Fresh Undiluted Human Mucus
by Samuel K. Lai and D. Elizabeth O'Hanlon and Suzanne Harrold and Stan T. Man and Ying-Ying Wang and Richard Cone and Justin Hanes
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 1/2007, Volume 104, Issue 5, pp. 1482 - 1487
Nanoparticles larger than the reported mesh-pore size range (10-200 nm) in mucus have been thought to be much too large to undergo rapid diffusional transport... 
Nanoparticles | Particle diffusion | Particle interactions | Medical treatment | Mucins | Mucus | Particle tracks | Particle trajectories | Polymers | Controlled release | Drug delivery | PEG | Mucosal tissues | Particle tracking | ANTIBODIES | mucosal tissues | MULTIPLE-PARTICLE TRACKING | MUCIN | MICROSPHERES | DRUG-DELIVERY | MULTIDISCIPLINARY SCIENCES | particle tracking | drug delivery | AIRWAY MUCUS | HUMAN CERVICAL-MUCUS | NANOSPHERES | SUSTAINED-RELEASE | CARCINOMA | Water | Nanoparticles - chemistry | Humans | Mucus - drug effects | Polyethylene Glycols - chemistry | Drug Carriers | Drug Delivery Systems | Particle Size | Biological Transport | Time Factors | Cervix Mucus - metabolism | Female | Mucus - metabolism | Polymers - chemistry | Diffusion | Drugs | Drug delivery systems | Research | Mucous membrane | Vehicles | Body fluids | Reproductive system | Index Medicus | Biological Sciences
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3. Full Text The Inner of the Two Muc2 Mucin-Dependent Mucus Layers in Colon Is Devoid of Bacteria
by Malin E. V. Johansson and Mia Phillipson and Joel Petersson and Anna Velcich and Lena Holm and Gunnar C. Hansson and Medicinska fakulteten and Medicinska och farmaceutiska vetenskapsområdet and Uppsala universitet and Institutionen för medicinsk cellbiologi and Integrativ Fysiologi
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 9/2008, Volume 105, Issue 39, pp. 15064 - 15069
We normally live in symbiosis with ∼10¹³ bacteria present in the colon. Among the several mechanisms maintaining the bacteria/host balance, there is limited... 
Proteins | Intestines | Gels | Protease inhibitors | Epithelial cells | Mucins | Bacteria | Goblets | Mucus | Mice | Colon cancer | Commensal bacteria | Large intestine | Proteomics | Ulcerative colitis | proteomics | colon cancer | MULTIDISCIPLINARY SCIENCES | GLYCOPROTEINS | SMALL-INTESTINE | GLYCOSYLATION | IDENTIFICATION | GEL | TERMINUS | COLITIS | ulcerative colitis | ENDOPLASMIC-RETICULUM | MICE | commensal bacteria | large intestine | LS 174T CELLS | Colon - cytology | Intestinal Mucosa - metabolism | Colitis - genetics | Rats | Colon - immunology | Intestinal Mucosa - microbiology | Rats, Sprague-Dawley | Colon - metabolism | Mucin-2 | Mucus - microbiology | Symbiosis - genetics | Intestinal Mucosa - cytology | Mucus - cytology | Animals | Colitis - microbiology | Intestinal Mucosa - immunology | Mice, Mutant Strains | Mucus - metabolism | Colon - microbiology | Mucus - immunology | Colitis - immunology | Mucins - physiology | Mucins - genetics | Development and progression | Microbiota (Symbiotic organisms) | Health aspects | Colon | Rodents | Cells | Index Medicus | Biological Sciences | Medical and Health Sciences | Medicin och hälsovetenskap | MEDICIN | MEDICINE
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4. Full Text Studies of mucus in mouse stomach, small intestine, and colon. I. Gastrointestinal mucus layers have different properties depending on location as well as over the Peyer's patches
by Ermund, Anna and Schütte, André and Johansson, Malin E.V and Gustafsson, Jenny K and Hansson, Gunnar C and Sahlgrenska akademin and Göteborgs universitet and Gothenburg University and Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi and Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology and Sahlgrenska Academy
American Journal of Physiology - Gastrointestinal and Liver Physiology, ISSN 0193-1857, 09/2013, Volume 305, Issue 5, pp. G341 - G347
Colon has been shown to have a two-layered mucus system where the inner layer is devoid of bacteria. However, a complete overview of the mouse gastrointestinal... 
Bacteria | Mucus thickness | Goblet cells | Mucin | Mucus adhesiveness | mucus adhesiveness | PHYSIOLOGY | bacteria | HOST | MICROBIOTA | M-CELLS | mucus thickness | goblet cells | EPITHELIUM | mucin | IN-VIVO | BACTERIAL OVERGROWTH | CYSTIC-FIBROSIS | SECRETION | ACCUMULATION | GASTROENTEROLOGY & HEPATOLOGY | Gastric Mucosa - microbiology | Intestinal Mucosa - metabolism | Peyer's Patches - metabolism | Colon - drug effects | Fluorescent Dyes - metabolism | Male | Microscopy, Video | Peyer's Patches - drug effects | Gastric Mucosa - metabolism | Intestinal Absorption | Intestinal Mucosa - drug effects | Time Factors | Gastric Mucosa - drug effects | Female | Mucus - metabolism | Dinoprostone - pharmacology | Adhesiveness | Intestine, Small - microbiology | Mice, Inbred C57BL | Intestinal Mucosa - microbiology | Permeability | Colon - metabolism | Mucus - microbiology | Carbachol - pharmacology | Microscopy, Confocal | Animals | Intestine, Small - drug effects | Colon - microbiology | Mice | Intestine, Small - metabolism | Physiological aspects | Mucus | Health aspects | Gastrointestinal system | Body fluids | Lipids | Physiology | Digestive system | Index Medicus | Mucosal Biology | Dinoprostone | Confocal | Gastric Mucosa | Intestine | pharmacology | Peyer's Patches | Colon | Intestinal Mucosa | Small | Cell and Molecular Biology | microbiology | drug effects | Inbred C57BL | Carbachol | Microscopy | Video | metabolism | Cell- och molekylärbiologi | Fluorescent Dyes
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5. Full Text Bacteriophage adhering to mucus provide a non-host-derived immunity
by Jeremy J. Barr and Rita Auro and Mike Furlan and Katrine L. Whiteson and Marcella L. Erb and Joe Pogliano and Aleksandr Stotland and Roland Wolkowicz and Andrew S. Cutting and Kelly S. Doran and Peter Salamon and Merry Youle and Forest Rohwer
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 6/2013, Volume 110, Issue 26, pp. 10771 - 10776
Mucosal surfaces are a main entry point for pathogens and the principal sites of defense against infection. Both bacteria and phage are associated with this... 
Bacteriophages | Symbiosis | Polysaccharides | Epithelial cells | Mucins | Cell lines | Bacteria | Mucus | Bacteriophage T4 | Pretreatment | Host-pathogen | Virus | Immunoglobulin | Immune system | immunoglobulin | MUCIN | MULTIDISCIPLINARY SCIENCES | host-pathogen | ESCHERICHIA-COLI | HUMAN GUT VIROME | OUTER CAPSID PROTEIN | virus | METAGENOMIC ANALYSIS | symbiosis | VIRAL COMMUNITIES | BACTERIAL SYMBIOSIS | IN-VIVO | immune system | CYSTIC-FIBROSIS | VIRUSES | Cell Line | Bacterial Adhesion - immunology | Bacteriophage T4 - immunology | Bacteriophage T4 - physiology | Adhesiveness | Humans | Mucus - virology | Symbiosis - immunology | Escherichia coli - immunology | Bacteriophage T4 - genetics | Bacteriophages - immunology | Host-Pathogen Interactions - immunology | Mucus - microbiology | Models, Immunological | Animals | Escherichia coli - virology | Bacteriophages - genetics | Mice | Mucus - immunology | Bacteriophages - physiology | Physiological aspects | Development and progression | Genetic aspects | Bacterial infections | Research | Epithelium | Tissue | Cell culture | Glycoproteins | Genomics | Index Medicus | Biological Sciences
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6. Full Text Helicobacter Pylori Moves through Mucus by Reducing Mucin Viscoelasticity
by Jonathan P. Celli and Bradley S. Turner and Nezam H. Afdhal and Sarah Keates and Ionita Ghiran and Ciaran P. Kelly and Randy H. Ewoldt and Gareth H. McKinley and Peter So and Shyamsunder Erramilli and Rama Bansil and Robert H. Austin
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 8/2009, Volume 106, Issue 34, pp. 14321 - 14326
The ulcer-causing gastric pathogen Helicobacter pylori is the only bacterium known to colonize the harsh acidic environment of the human stomach. H. pylori... 
Torque | Gels | Helicobacter pylori | Rheology | Mucins | pH | Gastric mucin | Bacteria | Mucus | Flagella | H. pylori | Bacterial motility | Gelation | gelation | COMPLEX FLUIDS | rheology | GNOTOBIOTIC PIGLETS | MULTIDISCIPLINARY SCIENCES | SOL-GEL TRANSITION | ESCHERICHIA-COLI | GASTROINTESTINAL MUCUS | VISCOUS ENVIRONMENT | PIG GASTRIC MUCUS | bacterial motility | FLAGELLAR MOTOR | BACTERIAL SWIMMING SPEED | ROTATION RATE | Viscosity | Fluoresceins - chemistry | Gastric Mucosa - microbiology | Elasticity | Gastric Mucins - chemistry | Gastric Mucosa - metabolism | Urea - chemistry | Mucus - microbiology | Gastric Mucins - metabolism | Animals | Helicobacter pylori - enzymology | Swine | Bacterial Proteins - metabolism | Mucus - metabolism | Urea - metabolism | Urease - metabolism | Helicobacter pylori - physiology | Microscopy, Fluorescence | Hydrogen-Ion Concentration | Physiological aspects | Motility | Research | Pathogens | Stomach | Translation | Fluorescent indicators | Urease | pH effects | Hydrolysis | Ammonia | Urea | mucin | viscoelasticity | Index Medicus | Biological Sciences | Physical Sciences
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7. Full Text Mucins and Their Role in Shaping the Functions of Mucus Barriers
by Wagner, C.E and Wheeler, K.M and Ribbeck, K
Annual Review of Cell and Developmental Biology, ISSN 1081-0706, 10/2018, Volume 34, Issue 1, pp. 189 - 215
We review what is currently understood about how the structure of the primary solid component of mucus, the glycoprotein mucin, gives rise to the mechanical... 
microbiological interactions | rheology | mucin | structure | permeability | mucus | COMPLEX FLUIDS | DRUG-DELIVERY | GASTROINTESTINAL MUCUS | DEVELOPMENTAL BIOLOGY | PIG GASTRIC MUCIN | CELL BIOLOGY | FIBROSIS AIRWAY SECRETIONS | IN-VITRO | HUMAN CERVICAL-MUCUS | CYSTIC-FIBROSIS | SALIVARY MUCINS | NANOPARTICLE DIFFUSION | Glycoproteins - genetics | Animals | Rheology | Humans | Mucin-1 - chemistry | Mucus - metabolism | Glycosylation | Mucus - chemistry | Structure-Activity Relationship | Permeability | Glycoproteins - chemistry | Mucin-1 - genetics | Physiological aspects | Mucins
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8. Full Text Biodegradable Polymer Nanoparticles That Rapidly Penetrate the Human Mucus Barrier
by Benjamin C. Tang and Michelle Dawson and Samuel K. Lai and Ying-Ying Wang and Jung Soo Suk and Ming Yang and Pamela Zeitlin and Michael P. Boyle and Jie Fu and Justin Hanes
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 11/2009, Volume 106, Issue 46, pp. 19268 - 19273
Protective mucus coatings typically trap and rapidly remove foreign particles from the eyes, gastrointestinal tract, airways, nasopharynx, and female... 
Nanoparticles | Biodegradation | Particle diffusion | Secretion | Sputum | Mucus | Coatings | Diffusion coefficient | Polymers | Latex | Mucus-penetrating particle | Cystic fibrosis | Drug delivery | Gene therapy | Mucosa | CLEARANCE | BIOADHESIVE | DEPOSITION | RETENTION | MULTIDISCIPLINARY SCIENCES | gene therapy | mucus-penetrating particle | CONTROLLED DRUG-DELIVERY | drug delivery | CERVICAL-MUCUS | PEGYLATED POLY(ANHYDRIDE) NANOPARTICLES | TRANSPORT | cystic fibrosis | PARTICLE TRACKING | IN-VIVO | mucosa | Polyethylene Glycols - metabolism | Anhydrides - chemistry | Drug Carriers - metabolism | Anhydrides - metabolism | Cervix Mucus - metabolism | Cystic Fibrosis - metabolism | Humans | Female | Polyethylene Glycols - chemistry | Drug Carriers - chemistry | Sputum - metabolism | Properties | Viscosity | Human subjects | Lungs | Biopolymers | Mucous membrane | Index Medicus | Biological Sciences
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9. Full Text Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis
by Le, Doan Thanh Lam and Tran, Thi-Ly and Duviau, Marie-Pierre and Meyrand, Mickael and Guérardel, Yann and Castelain, Mickaël and Loubière, Pascal and Chapot-Chartier, Marie-Pierre and Dague, Etienne and Mercier-Bonin, Muriel
PLoS ONE, ISSN 1932-6203, 11/2013, Volume 8, Issue 11, pp. e79850 - e79850
Adhesion of bacteria to mucus may favor their persistence within the gut and their beneficial effects to the host. Interactions between pig gastric mucin (PGM)... 
GRAM-POSITIVE BACTERIA | HUMAN INTESTINAL MUCUS | IN-VITRO | FORCE SPECTROSCOPY | FLOW CHAMBER | MULTIDISCIPLINARY SCIENCES | GLYCOPROTEINS | HUMAN COLONIC MUCIN | LACTOBACILLI | COLONIZATION | IDENTIFICATION | Gastric Mucins - metabolism | Animals | Carrier Proteins - metabolism | Bacterial Adhesion - physiology | Swine | Surface Properties | Lactococcus lactis - metabolism | Mucus - metabolism | Membrane Proteins - metabolism | Mucus - physiology | Mucus - microbiology | Atomic force microscopy | Protein binding | Lactococcus lactis | Shear flow | Genes | Gram-positive bacteria | Mucus | Defects | Streptococcus infections | Proteins | Microbiota | Adhesive strength | Bacteria | Binding | Bacterial infections | Oligosaccharides | Sedimentation | Low level | Adhesion | Glycan | Mutants | Flow | Probiotics | Detachment | Adhesives | Mucin | Pili | Microscopy | Index Medicus | Life Sciences
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10. Full Text Nanoparticle diffusion in respiratory mucus from humans without lung disease
by Schuster, Benjamin S and Suk, Jung Soo and Woodworth, Graeme F and Hanes, Justin
Biomaterials, ISSN 0142-9612, 2013, Volume 34, Issue 13, pp. 3439 - 3446
Abstract A major role of respiratory mucus is to trap inhaled particles, including pathogens and environmental particulates, to limit body exposure. Despite... 
Advanced Basic Science | Dentistry | Inhaled nanoparticles | Multiple particle tracking | Mucus-penetrating particles | Rheology | Respiratory mucus | DRUG | MULTIPLE-PARTICLE TRACKING | MATERIALS SCIENCE, BIOMATERIALS | PULMONARY DELIVERY | ENGINEERING, BIOMEDICAL | BARRIER | PENETRATION | RAPID-TRANSPORT | AIRWAY SURFACTANT | CYSTIC-FIBROSIS SPUTUM | MICROSTRUCTURE | Viscosity | Microscopy, Electron, Scanning | Lung Diseases - metabolism | Biological Transport | Nanoparticles - chemistry | Time Factors | Humans | Mucus - metabolism | Lung - metabolism | Lung Diseases - pathology | Diffusion | Air pollution | Polyols | Lung diseases | Biomedical engineering | Index Medicus | Human | Nanoparticles | Lungs | Polyethylene glycol | Particulates | Rheometers | Mucus | Nanostructure
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