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2003, 2nd ed., ISBN 0123617766, xviii, 316
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of... 
Affinage | Protéines membranaires | Purification | Membrane proteins | Biology, life sciences
Book
1994, Separation, detection, and characterization of biological macromolecules, ISBN 9780127255507, xvii, 166
Book
Methods in Molecular Biology, ISSN 1064-3745, 08/2016, Volume 1432, pp. 261 - 267
Membrane proteins are localized within a lipid bilayer; in order to purify them for functional and structural studies the first step must involve solubilizing... 
Nanodisc | Detergent | Purification | Polymer | Solubilization | SMALP | Membrane proteins | Lipid Bilayers - analysis | Membrane Proteins - isolation & purification | Chromatography, Affinity | Maleates - chemistry | Membrane Proteins - chemistry | Polystyrenes - chemistry
Journal Article
Advances in Experimental Medicine and Biology, ISSN 0065-2598, 2016, Volume 922, pp. 13 - 28
Journal Article
2016, Advances in Experimental Medicine and Biology, Volume 922, 16
Detergents play a significant role in structural and functional characterisation of integral membrane proteins (IMPs). IMPs reside in the biological membranes... 
MEDICINE, RESEARCH & EXPERIMENTAL | Crystallisation | SOLUBILIZATION | MECHANISM | Protein purification | STABILIZATION | STABILITY | Lipids | BARRIERS | FACIAL AMPHIPHILES | Detergents | Membrane proteins | MIXTURES | LIPID-BILAYERS | Micelles | BIOLOGY | LIPOSOMES
Book Chapter
PLoS ONE, ISSN 1932-6203, 05/2017, Volume 12, Issue 5, p. e0178246
There are many strategies to purify recombinant proteins of interest, and affinity purification utilizing monoclonal antibody that targets a linear epitope... 
COMPLEX | RECOMBINANT PROTEINS | CRYSTAL-STRUCTURE | MULTIDISCIPLINARY SCIENCES | ESCHERICHIA-COLI | COUPLED RECEPTOR | ANTIBODY | STEP PURIFICATION | AFFINITY TAG | IMMOBILIZATION | PEPTIDE | Cricetulus | Deubiquitinating Enzyme CYLD | Humans | Immunoblotting | Epitopes - immunology | Recombinant Proteins - isolation & purification | MCF-7 Cells | HEK293 Cells | Membrane Proteins - metabolism | Sequence Tagged Sites | Antibodies, Monoclonal - immunology | Chromatography, Affinity - methods | CHO Cells | Rabbits | Membrane Proteins - isolation & purification | Tumor Suppressor Proteins - metabolism | Epitopes - genetics | Receptors, Dopamine D1 - immunology | Animals | Carrier Proteins - metabolism | Cell-Free System | Carrier Proteins - isolation & purification | Tumor Suppressor Proteins - isolation & purification | HeLa Cells | Amino Acid Substitution | Receptors, Dopamine D1 - genetics | Proteins | Monoclonal antibodies | Usage | Separation | Research | Methods | Cell culture | Peptides | Biochemistry | Nucleotides | Assaying | Crystallography | Amino acid substitution | Sample preparation | Mathematical analysis | Assembly | Deoxyribonucleic acid--DNA | Medical research | Enzymes | Antigens | Elution | Construction | Stability | Protein biosynthesis | Embryos | Membrane proteins | Discrimination | Mutagenesis | Protein synthesis | Human performance | Stem cells | Affinity | Wheat | Health care | Calcium | Science | Antibodies | Amino acids | Biology | Suicide | Engineering | Ethylenediaminetetraacetic acids | Clonal deletion | Affinity chromatography | pH | Attenuation | Ion exchange | Technology utilization | Expression vectors | Crystal structure | Dopamine | Purification | Extinction | Research & development--R&D | Cloning | C-Terminus | Chromatography | Medicine | Buffers (chemistry) | Acids | Plasmids | Reagents | Proteomics | Tagging | Adrenergic receptors | Genetic engineering | Research & development | R&D | Deoxyribonucleic acid | DNA
Journal Article
Advances in experimental medicine and biology, ISSN 0065-2598, 2016, Volume 922, p. 13
Detergents play a significant role in structural and functional characterisation of integral membrane proteins (IMPs). IMPs reside in the biological membranes... 
Detergents - pharmacology | Membrane Proteins - isolation & purification | Humans | Molecular Conformation | Crystallization | Solubility | Crystallography, X-Ray | Membrane Lipids - chemistry | Animals | Lipid Bilayers | Membrane Proteins - chemistry | Micelles | Chemical Phenomena | Hydrophobic and Hydrophilic Interactions
Journal Article
Journal of Chromatography A, ISSN 0021-9673, 2011, Volume 1218, Issue 17, pp. 2396 - 2401
Approximately 25 cm × 25 cm large sheets of crosslinked highly porous poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate-co-ethylhexyl methacrylate)... 
Membranes | PolyHIPE | Poly(glycidyl methacrylate) | Protein purification | CHEMISTRY, ANALYTICAL | MONOLITHS | VOLUME | SIZE | BIOCHEMICAL RESEARCH METHODS | ION-EXCHANGE | ADSORBERS | SEPARATION | SCAFFOLDS | Albumin | Chromatography
Journal Article
Applied Surface Science, ISSN 0169-4332, 07/2014, Volume 308, pp. 155 - 160
Journal Article
Journal of Chromatography A, ISSN 0021-9673, 08/2014, Volume 1355, pp. 143 - 148
Journal Article
Biotechnology and Bioengineering, ISSN 0006-3592, 10/2014, Volume 111, Issue 10, pp. 2019 - 2026
ABSTRACT We describe a new affinity purification tag called Car9 that confers proteins to which it is fused micromolar affinity for unmodified silica. When... 
fusion protein | affinity tag | proteolytic cleavage | purification | silica | Purification | Affinity tag | Fusion protein | Silica | Proteolytic cleavage | ESCHERICHIA-COLI | OMPT | MEMBRANE-PROTEIN | ADSORPTION BEHAVIOR | STRATEGIES | PEPTIDES | REMOVAL | BIOTECHNOLOGY & APPLIED MICROBIOLOGY | FLUORESCENT PROTEIN | EXPRESSION | SURFACES | Recombinant Fusion Proteins - isolation & purification | Peptide Hydrolases - genetics | Peptides - genetics | Green Fluorescent Proteins - genetics | Recombinant Fusion Proteins - metabolism | Silicon Dioxide - metabolism | Peptides - metabolism | Maltose-Binding Proteins - isolation & purification | Escherichia coli - metabolism | Maltose-Binding Proteins - genetics | Bacterial Outer Membrane Proteins - genetics | Chromatography, Affinity - methods | Peptide Hydrolases - metabolism | Green Fluorescent Proteins - isolation & purification | Green Fluorescent Proteins - metabolism | Escherichia coli Proteins - metabolism | Maltose-Binding Proteins - metabolism | Hydrolysis | Peptides - isolation & purification | Bacterial Outer Membrane Proteins - metabolism | Escherichia coli - genetics | Escherichia coli Proteins - genetics | Protein Binding | Recombinant Fusion Proteins - genetics | Luminescent Proteins - genetics | Luminescent Proteins - isolation & purification | Luminescent Proteins - metabolism | Proteins | Adsorption | Proteases | Proteolysis | Escherichia coli | Liquid chromatography | Sugars | Protein binding | Pressure drop | Affinity | Silica gel | Devices | Silicon dioxide
Journal Article
Journal of Chromatography A, ISSN 0021-9673, 10/2017, Volume 1521, pp. 19 - 26
Fabricating membrane adsorbers with high adsorption capacity and appreciable throughput for the separation and purification of protein products is challenging... 
Microfiltration | Membrane chromatography | Alginate dialdehyde | Protein purification | Lysozyme | CLEARANCE | CHEMISTRY, ANALYTICAL | STEP-PURIFICATION | DESIGN | VIRUS | BIOCHEMICAL RESEARCH METHODS | MONOCLONAL-ANTIBODY | CHROMATOGRAPHY | BEADS | CRYOGEL | SEPARATION | Adsorption | Sulfites | Nylon | Chemical tests and reagents | Chromatography
Journal Article
Chemistry of Materials, ISSN 0897-4756, 02/2018, Volume 30, Issue 4, pp. 1265 - 1276
Hydrogels are promising materials as membrane selective layers due to their fouling-resistant nature, tunable mesh size, and functionalizability. These... 
FILM COMPOSITE MEMBRANES | PHASE-SEPARATION | OIL/WATER SEPARATION | MATERIALS SCIENCE, MULTIDISCIPLINARY | ULTRAFILTRATION MEMBRANES | CROSS-LINKING | CHEMISTRY, PHYSICAL | UF MEMBRANES | MICROFILTRATION MEMBRANES | POLY(ETHYLENE OXIDE) | DRINKING-WATER | ACTIVE LAYERS
Journal Article