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FEBS Letters, ISSN 0014-5793, 2015, Volume 589, Issue 7, pp. 789 - 797
•Site-directed mutations of AUS1 around the CuA site were generated and verified... 
Aurone synthase (AUS) | 4-Deoxyaurone | Polyphenol oxidase (PPO) | Copper binding site | Type-3 copper protein | Site-directed mutagenesis | poly(oxy-1,4-phenylsulfonyl-1,4-phenyl) | catechol oxidase from Vitis vinifera (protein) | F-AAS | PHC | liquid chromatography / mass spectrometry | aurone synthase from Coreopsis grandiflora (protein) | PPO | THC | GF-AAS | TBC | bmTYR | tyrosinase from Bacillus megaterium (protein) | polyphenol oxidase | 4-tert-butylcatechol | vvCO | 2′,3,4,4′,6′-pentahydroxychalcone | tyrosinase from Aspergillus oryzae (protein) | ultra high performance liquid chromatography–electrospray tandem mass spectrometry | GST | catechol oxidase from Ipomoea batatas (protein) | liquid chromatography | isopropyl-β-d-thiogalactopyranoside | cgAUS1 | aurone synthase from Coreopsis grandiflora (gene) | LC/MS | atomic absorption spectroscopy | AAS | ibCO | PPO-6 | AmAS1 | aoTYR | PMSF | glutathione S-transferase | nanoUHPLC–ESI-MS/MS | dandelion PPO-6 from Taraxacum officinale (protein) | 2′,4,4′,6′-tetrahydroxychalcone | IPTG | phenylmethylsulfonylfluorid | flame atomic absorption spectroscopy | aureusidin synthase from Antirrhinum majus (protein) | graphite furnace atomic absorption spectroscopy | PES | PLANT | CRYSTAL-STRUCTURE | CATECHOL OXIDASE | BIOCHEMISTRY & MOLECULAR BIOLOGY | ESCHERICHIA-COLI | ASPERGILLUS-ORYZAE | CELL BIOLOGY | TYROSINASE GENE | BIOPHYSICS | CLONING | FUNGAL | AGARICUS-BISPORUS | EXPRESSION | Chalcones - metabolism | Plant Proteins - genetics | Mutagenesis, Site-Directed | Catechol Oxidase - metabolism | Copper - metabolism | Coreopsis - genetics | Alanine - metabolism | Catechol Oxidase - genetics | Cysteine - metabolism | Plant Proteins - metabolism | Binding Sites | Coreopsis - enzymology | Oxidases | Index Medicus | PPO, polyphenol oxidase | bmTYR, tyrosinase from Bacillus megaterium (protein) | MS, liquid chromatography | GF-AAS, graphite furnace atomic absorption spectroscopy | PPO-6, dandelion PPO-6 from Taraxacum officinale (protein) | PES, poly(oxy-1,4-phenylsulfonyl-1,4-phenyl) | ibCO, catechol oxidase from Ipomoea batatas (protein) | LC, liquid chromatography | nanoUHPLC–ESI-MS | mass spectrometry | AAS, atomic absorption spectroscopy | cgAUS1, aurone synthase from Coreopsis grandiflora (protein) | AmAS1, aureusidin synthase from Antirrhinum majus (protein) | F-AAS, flame atomic absorption spectroscopy | GST, glutathione S-transferase | IPTG, isopropyl-β-d-thiogalactopyranoside | PMSF, phenylmethylsulfonylfluorid | aoTYR, tyrosinase from Aspergillus oryzae (protein) | vvCO, catechol oxidase from Vitis vinifera (protein) | MS, ultra high performance liquid chromatography–electrospray tandem mass spectrometry | PHC, 2′,3,4,4′,6′-pentahydroxychalcone | TBC, 4-tert-butylcatechol | THC, 2′,4,4′,6′-tetrahydroxychalcone | cgAUS1, aurone synthase from Coreopsis grandiflora (gene)
Journal Article
BMC biotechnology, ISSN 1472-6750, 2008, Volume 8, Issue 1, pp. 91 - 91
Journal Article
Journal Article
Journal of Biological Chemistry, ISSN 0021-9258, 02/2008, Volume 283, Issue 6, pp. 3256 - 3263
Journal Article
Angewandte Chemie (International ed.), ISSN 1433-7851, 2019, Volume 58, Issue 26, pp. 8757 - 8761
.... To test these hypotheses, we performed site‐directed mutagenesis studies and phylogenetic analyses with dandelion PPOs offering high phylogenetic diversity, the results of which refute the structure‐based hypotheses... 
oxidoreductases | enzyme engineering | mutagenesis | metalloenzymes | substrate specificity | PLANT | DIFFERENTIAL EXPRESSION | POLYPHENOL OXIDASE | CLONING | CRYSTAL-STRUCTURE | MULTIGENE FAMILY | CHEMISTRY, MULTIDISCIPLINARY | Oxidases | Phylogeny | Hydroxylation | Structure | Metalloenzymes | Crystals | PPOs | Catechol oxidase | Catechol | Diphenols | Substrates | Quinones | Hypotheses | Mutagenesis | Substrate specificity | Classification | Phylogenetics | Oxidation | Tyrosinase | Crystal structure | Index Medicus
Journal Article
Microbial cell factories, ISSN 1475-2859, 2018, Volume 17, Issue 1, pp. 141 - 13
.... Structure-based site-directed saturation mutagenesis of KsdD3 was performed with W299 mutants, which resulted in improved catalytic activities toward various steroidal substrates... 
3-ketosteroid | Rational design | Dehydrogenase | Saturation mutagenesis | Steered molecular dynamics | Substrate Specificity | Mutagenesis, Site-Directed - methods | Ketosteroids - metabolism | Oxidoreductases - metabolism | Biotransformation | Genetic aspects | Actinomycetes | Oxidoreductases | Gene mutations | Properties | Molecular dynamics | Enzymes | Catalysis | Comparative analysis | Chemical synthesis | Force and energy
Journal Article