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Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 6/2010, Volume 107, Issue 26, pp. 12034 - 12039
Site-directed mutagenesis in higher plants remains a significant technical challenge for basic research and molecular breeding. Here, we demonstrate... 
Polymerase chain reaction | Mutagenesis | DNA | Genomics | Site directed mutagenesis | Genomes | Plants | Genetic mutation | Zinc | Seedlings | Nonhomologous end joining | Ku80 | Mutation | dsDNA breaks | SEQUENCES | MULTIDISCIPLINARY SCIENCES | DOUBLE-STRAND BREAKS | RESTRICTION ENZYMES | PLANT HORMONE ABA | CLEAVAGE | SUGAR | mutation | REPAIR | GENE | nonhomologous end joining | HOMOLOGOUS RECOMBINATION | RICE | Recombinant Proteins - metabolism | Amino Acid Sequence | Promoter Regions, Genetic | Arabidopsis Proteins - genetics | Mutagenesis, Site-Directed | Genes, Plant | Molecular Sequence Data | Recombinant Proteins - genetics | DNA Repair - genetics | Transcription Factors - genetics | DNA, Plant - metabolism | DNA Breaks, Double-Stranded | Deoxyribonucleases - metabolism | Arabidopsis - metabolism | Arabidopsis - genetics | Arabidopsis Proteins - metabolism | DNA Helicases - metabolism | Deoxyribonucleases - genetics | Base Sequence | Plants, Genetically Modified | Protein Engineering | DNA Helicases - genetics | DNA, Plant - genetics | Arabidopsis thaliana | Gene mutations | Plant genetics | Genetic aspects | Zinc finger proteins | Research | Properties | Identification and classification | Proteins | Flowers & plants | Gene expression | DNA repair | Cells | Index Medicus | Data processing | ABI4 gene | Glucose | Gene deletion | Promoters | Homozygotes | Plant breeding | Somatic cells | Site-directed mutagenesis | Point mutation | Nuclease | Biological Sciences
Journal Article
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), ISSN 0302-9743, 2018, Volume 11088, pp. 219 - 230
Conference Proceeding
Nucleic acids research, ISSN 0305-1048, 2004, Volume 32, Issue 14, pp. e115 - e115
We have developed a new primer design method based on the QuickChange(TM) site-directed mutagenesis protocol, which significantly improves the PCR... 
EVOLUTION | PROTEIN | BIOCHEMISTRY & MOLECULAR BIOLOGY | PCR | Mutagenesis, Site-Directed | Gene Library | Protein Engineering - methods | Electrophoresis, Agar Gel | Polymerase Chain Reaction | Directed Molecular Evolution | DNA Primers | Index Medicus | NAR Methods Online
Journal Article
Journal Article
Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, 1/2007, Volume 104, Issue 2, pp. 491 - 494
Journal Article
ACS Catalysis, ISSN 2155-5435, 03/2019, Volume 9, Issue 3, pp. 2068 - 2078
In this work, we extended the generic approach for the site directed immobilization of enzymes based on maleimide \ thiol coupling of engineered enzymes to the... 
site-directed mutagenesis | bilirubin oxidase | immobilization | maleimide | oxygen reduction | direct electron transfer | MYROTHECIUM-VERRUCARIA | OXIDATION | CHEMISTRY, PHYSICAL | CARBON NANOTUBES | MULTICOPPER OXIDASES | COPPER CENTERS | ENZYME | DIRECT ELECTROCHEMISTRY | DIRECT ELECTRON-TRANSFER | PROTON-TRANSFER | VOLTAMMETRY | Material chemistry | Chemical Sciences | Catalysis
Journal Article
MOLECULES, ISSN 1420-3049, 01/2019, Volume 24, Issue 1
The -glycoside hydrolases (LXYL-P1-1 and LXYL-P1-2) from Lentinula edodes (strain M95.33) can specifically hydrolyze 7--xylosyl-10-deacetyltaxol (XDT) to form... 
site-directed mutagenesis | XYLANASE | glucosidase | xylosidase | STABILITY | STRATEGY | BIOCHEMISTRY & MOLECULAR BIOLOGY | CHEMISTRY, MULTIDISCIPLINARY | BIOCATALYSIS | molecular docking | EVOLUTION | 7-BETA-XYLOSYL-10-DEACETYLTAXOL | GEOBACILLUS-STEAROTHERMOPHILUS | BACILLUS-SUBTILIS | TAXOL | catalytic efficiency
Journal Article
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, ISSN 1422-0067, 02/2019, Volume 20, Issue 4, p. 916
Journal Article
Journal Article